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In this paper, finite element calculations are used to simulate the melting flow pattern within a titanium/steel dissimilar welded joint with Cu as the transition layer under an oscillating laser welding process, and these calculations are used as a guide for bias oscillating laser welding experiments. When using 304SS side bias oscillating laser welding, welded joints with a tensile strength of 395 MPa were obtained. Compared to the linear bias welding process, it was found that the bias oscillation laser welding process can, on the one hand, generate a stirring effect on the melting pool during the welding process, forming Fe-rich structures encapsulated by Cu layers, which in turn strengthen the Cu layers, and on the other hand, effectively reduce the content of Fe-Ti brittle intermetallic compounds in the weld and obtain a Cu-Ti IMC layer with a thickness of only 10 μm, thus significantly improving the tensile strength of the weld joint and reducing the possibility of incidental fracture. [ABSTRACT FROM AUTHOR]

Phosphorus (P) limitation of ecosystem processes is widespread in terrestrial habitats. While a few auxiliary metabolic genes (AMGs) in bacteriophages from aquatic habitats are reported to have the potential to enhance P-acquisition ability of their hosts, little is known about the diversity and potential ecological function of P-acquisition genes encoded by terrestrial bacteriophages. Here, we analyze 333 soil metagenomes from five terrestrial habitat types across China and identify 75 viral operational taxonomic units (vOTUs) that encode 105 P-acquisition AMGs. These AMGs span 17 distinct functional genes involved in four primary processes of microbial P-acquisition. Among them, over 60% (11/17) have not been reported previously. We experimentally verify in-vitro enzymatic activities of two pyrophosphatases and one alkaline phosphatase encoded by P-acquisition vOTUs. Thirty-six percent of the 75 P-acquisition vOTUs are detectable in a published global topsoil metagenome dataset. Further analyses reveal that, under certain circumstances, the identified P-acquisition AMGs have a greater influence on soil P availability and are more dominant in soil metatranscriptomes than their corresponding bacterial genes. Overall, our results reinforce the necessity of incorporating viral contributions into biogeochemical P cycling. Soil viruses could have a disproportionate role in ecosystem functioning. Here, the authors combine multiple data and approaches to explore diversity and distribution of P-acquisition genes encoded by soil bacteriophages, suggesting the importance of viral auxiliary metabolism for nutrient cycling. [ABSTRACT FROM AUTHOR]

ER5183 filler wire is used as filler metal for CMT MIX + Synchropulse welding of 6063 aluminum alloy sheets. The relationships among the fusion zone (FZ) size, pore characteristics, microstructure and mechanical properties are studied. The results show that the heat and convection intensity of the molten pool increase as the heat input increases, so that more base metal is melted and the weld width increases. The porosity and chain-like pores are closely related to the FZ size. The FZ size characteristic index (k) is established to predict the porosity and chain-like pores. The lower the k value is, the more difficult chain-like pores are to generate. Properly increasing the heat input eliminates the chain-like pores without aggravating the softening. The chain-like pores enormously reduce the effective load-bearing area of the FZ, which leads to the pretty poor mechanical properties of the FZ. Considering that the high porosity weakens the fatigue life, it is recommended to properly increase the heat input to improve the service performance of aluminum alloy welded joints on the premise that the full penetration welded joint has been obtained. [ABSTRACT FROM AUTHOR]

Laser welding was applied to three types of dual-phase (DP) steel sheets (DP590, DP780, and DP980) with different martensite contents. The microstructure of laser welded joints was observed by optical microscope and scanning electron microscope, and the hardness of welded joints was measured by a microhardness tester. The tensile testing machine and the Erichsen test machine were employed to measure the tensile properties and forming properties of welded joints, respectively. The microstructure model showed that the welded joints of three different materials all contained a hardened zone (HZ) that was composed of a fusion zone, a coarse-grained heat-affected zone, and fine-grained heat-affected zone. The microstructure of the HZ contained mainly martensite such that the average martensite content of welded joints was increased by 1.9-9.8%, and the microhardness of the HZ was 1.2-1.9 times that of the base metal (BM). Moreover, the elongation of welded joints decreased with decreasing martensite content in the BM, and the effect was severe for the DP590 welded joint (21.8%) compared to the other two welded joints. During the Erichsen test, deformation failure varied among the welded joints with different martensite contents, and it was determined by the HZ and softened zone. The fracture occurred in the softened zone and ran parallel along the zone for DP980 welded joints, whereas the fracture occurred in the HZ and ran perpendicular to this zone for the other two welded joints. The Erichsen values of the DP590, DP780, and DP980 welded joints were 81.1, 91.7, and 85.0% of the BM, respectively. [ABSTRACT FROM AUTHOR]

The joining of 14.8 mm thick pipeline steels was achieved with developments in high-power fiber laser by laser welding or hybrid laser arc welding. However, there were few studies on the effects of single-pass welding heat input on the M-A component and impact toughness of the heat-affected zone. In this study, single-pass welding thermal cycle was performed on Gleeble 3800 thermal simulation tester; the effect of heat input on microstructure, M-A constituent, hardness, toughness and corrosion resistance of coarse-grained heat-affected zone of X100 pipeline steel was studied. The results showed: The microstructure was lath martensite with a heat input of less than 8 kJ/cm. When the heat input was 26-36 kJ/cm, the microstructure was granular bainite. The heat input had not obvious effect on hardness (only 16% hardness reduction in this heat input arrange). However, the impact toughness was strongly influenced by heat input, with an 88% hardness reduction compared to base metal, due to M-A constituent and prior austenite grain size. Few finer M-A constituent dispersing in austenite was obtained instead of necklace-like M-A constituent at the prior austenite grain boundary when the welding heat input was less than 15 kJ/cm, which was beneficial to improve the impact toughness and corrosion resistance of welded joints of X100 pipeline steels. [ABSTRACT FROM AUTHOR]

Background: As a potential tumor suppressor gene, Claudin-7 (Cldn7), which is a component of tight junctions, may play an important role in colorectal cancer occurrence and development.Aims: To generate a knockout mouse model of inducible conditional Cldn7 in the intestine and analyze the phenotype of the mice after induction with tamoxifen.Methods: We constructed Cldn7-flox transgenic mice and crossed them with Villin-CreERT2 mice. The Cldn7 inducible conditional knockout mice appeared normal and were well developed at birth. We induced Cldn7 gene deletion by injecting different dosages of tamoxifen into the mice and then conducted a further phenotypic analysis.Results: After induction for 5 days in succession at a dose of 200 µl tamoxifen in sunflower oil at 10 mg/ml per mouse every time, the mice appeared dehydrated, had a lower temperature, and displayed inactivity or death. The results of hematoxylin-eosin staining showed that the intestines of the Cldn7 inducible conditional knockout mice had severe intestinal defects that included epithelial cell sloughing, necrosis, inflammation and hyperplasia. Owing to the death of ICKO mice, we adjusted the dose of tamoxifen to a dose of 100 µl in sunflower oil at 10 mg/ml per mouse (aged more than 8 weeks old) every 4 days. And we could induce atypical hyperplasia and adenoma in the intestine. Immunofluorescent staining indicated that the intestinal epithelial structure was destroyed. Electron microscopy experimental analysis indicated that the intercellular gap along the basolateral membrane of Cldn7 inducible conditional knockout mice in the intestine was increased and that contact between the cells and matrix was loosened.Conclusions: We generated a model of intestinal Cldn7 inducible conditional knockout mice. Intestinal Cldn7 deletion induced by tamoxifen initiated inflammation and hyperplasia in mice. [ABSTRACT FROM AUTHOR]

Osteocytes were considered as potential sensors of mechanical loading and orchestrate the bone remodeling adapted to mechanical loading. On the other hand, osteocytes are also considered as the unloading sensors in vivo. Previous studies showed that the mechanosensation and mechanotransduction of osteocytes may play an essential role in mediating bone response to microgravity, and one of the most important molecular signaling pathway involved in the mechanotransduction is the Wnt/ ß-catenin signaling pathway. In order to investigate the effect of simulated microgravity on the Wnt/ ß-catenin signaling pathway in osteocytes, MLO-Y4 cells (an osteocyte-like cell line) were cultured under controlled rotation to simulate microgravity for 5 days. The cytoskeleton and ß-catenin nuclear translocation of MLO-Y4 cells were detected by laser scanning confocal microscope and the fluorescence intensity was quantified; the mRNA expressions of upstream and downstream key components in Wnt canonical signaling were detected with RT-PCR. Two regulators of the Wnt/ ß-catenin pathway, NMP4/CIZ and Smads, were also investigated by RT-PCR; finally the expression of Wnt target genes and Sost protein level were detected with the absence or presence of the Sclerostin antibody (Scl-AbI) under simulated microgravity. The results showed that under simulated microgravity, (1) F-actin filaments were disassembled and some short dendritic processes appeared at the cell periphery; (2) the gene expression of Wnt3a, Wnt5a, DKK1, CyclinD1, LEF-1 and CX43 in the simulated microgravity group were significantly lower whereas Wnt1 and Sost in the simulated microgravity group were significantly higher than the control group; (3) the gene and protein level of ß-catenin were reduced, and no ß-catenin nuclear translocation observed; (4) the gene expression of Smad1, Smad4 and Smad7 were significantly lower whereas NMP4/CIZ and Smad3 in the simulated microgravity were significantly higher than the control group; (5) Scl-AbI partially inhibited the down-regulation of simulated microgravity to Wnt target gene expression and Sclerostin protein expression. The results suggested that firstly the cytoskeleton was disturbed in MLO-Y4 by simulated microgravity; secondly the activity of Wnt/ ß-catenin signaling pathway was depressed, with the nuclear translocation of ß-catenin suppressed by simulated microgravity; thirdly the Wnt/ ß-catenin signaling pathway positive regulators (Smads) were decreased, while the negative regulator (NMP4/CIZ) was increased under simulated microgravity; finally Scl-AbI could partially restore the adverse effect of simulated microgravity to Wnt signaling. This study may help us to understand the mechanotransduction alteration of Wnt/ ß-catenin signaling pathway in osteocytes under simulated microgravity, and further may partly clarify the mechanism of microgravity-induced osteoporosis. [ABSTRACT FROM AUTHOR]

The effect of yield-to-tensile strength ratio ( Y/T) on failure pressure of X70 pipeline without and with corrosion defects was investigated. The stress-strain response of materials was characterized by a power-law hardening curve. Two formulas to estimate the strain hardening exponent n for a special Y/T were obtained by least squared regression method and the influence of Y/T on n was analyzed. As an application of n- Y/T expression, the analytical solutions of burst pressure for X70 pipeline without and with corrosion defects were also obtained. The results indicate that the burst pressure of defect-free X70 pipe without corrosion defects is a function of the Y/T, pipe geometry t/ D and engineering tensile strength, and increases as Y/T or t/ D increases; whilst the burst pressure of corroded X70 pipe decreases with the increase of defect depths, d/ t. Comparisons indicate that the present analytical solutions closely match available experimental and numerical data. [ABSTRACT FROM AUTHOR]

Superconducting MgB2 thick film has been prepared via hybrid physical-chemical vapor deposition method on Al2O3 (0001) substrate by using B2H6 and magnesium ingot as raw materials reacted from 730 to 830°C for 40 min under 20 to 30 kPa. Its thickness is about 40 µm. The MgB2 thick film shows Tc (onset) = 39.0 K and Tc (0) = 37.2 K. X-ray diffraction pattern shows that the film grown along (101) direction has small amount of impurities of Mg and MgO. Scanning electron microscopy and energy dispersive X-ray spectroscopy indicated that these impurities existed indeed and were Mg rich. The MgO film was formed on the surface of the MgB2 thick film to further protect the sample from oxidation. We presented a new mechanism for the formation of the thick film. [ABSTRACT FROM AUTHOR]