1. SGOL1 variant B induces abnormal mitosis and resistance to taxane in non-small cell lung cancers.
- Author
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Shun Matsuura, Tomoaki Kahyo, Kazuya Shinmura, Moriya Iwaizumi, Hidetaka Yamada, Kazuhito Funai, Jun Kobayashi, Masayuki Tanahashi, Hiroshi Niwa, Hiroshi Ogawa, Takashi Takahashi, Naoki Inui, Takafumi Suda, Kingo Chida, Yoshinori Watanabe, and Haruhiko Sugimura
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MITOSIS , *LUNG cancer , *TAXANES , *CELL cycle , *CHROMATIDS , *GENE expression - Abstract
Mitosis is the most conspicuous cell cycle phase and Shugoshin-like 1 (SGOL1) is a key protein in protecting sister chromatids from precocious separation during mitosis. We studied the role of SGOL1 and its splice variants in non-small cell lung cancer (NSCLC) using 82 frozen NSCLC tissue samples. SGOL1-B expression was prevalent in smokers, in cases with a wild-type (WT) EGFR status, and in cases with the focal copy number amplification of genes that are known to be important for defining the biological behaviors of NSCLC. The overexpression of SGOL1-B1 in an NSCLC cell line induced aberrant chromosome missegregation, precociously separated chromatids, and delayed mitotic progression. A higher level of SGOL1-B mRNA was related to taxane resistance, while the forced down-regulation of SGOL1-B increased the sensitivity to taxane. These results suggest that the expression of SGOL1-B causes abnormal mitosis and taxane resistance in NSCLC cells. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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