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Global food production predominantly depends on a limited number of cereal crops; however, numerous other crops have the potential to support the nutrition and economy of many local communities in developing countries. The different crop species characterized as having relatively low perceived economic importance or agricultural significance are known as underutilized crops. Millet is one of the underutilized crops with significant potential to address nutrient and hunger-related challenges in many developing countries like Nepal due to its versatility and climate resilience. Little is known about the determinants of adoption, cultural importance, and nutritional benefits of millet. Therefore, this study uses data from 1988 to 2019 and examines trends in millet production and its climate resilience, employing the Dynamic Ordinary Least Square Method. Results indicate that fertilizer use, cultivated area, and rural population significantly impact millet production in Nepal, while mean temperature has a negative but insignificant effect. The findings suggest that climate change does not significantly impact millet production. Millet is well adapted to challenging environments and offers superior nutritional value, suggesting that integration of millet into modern agriculture could be a valuable tool for creating a more sustainable, equitable, resilient, and healthy agrifood system that benefits both people and the planet. This research provides valuable insights for policymakers to enhance underutilized crops such as millet and implement strategies to integrate them into central agrifood systems. It also has a more considerable socio-economic impact on local communities. [ABSTRACT FROM AUTHOR]

Bioethanol production from cereal straw is a sustainable alternative to meet the current energy demand, but its low nitrogen (N) content and high carbon to nitrogen (C/N) ratio are problematic during bioethanol processing. Intercropping cereals with legumes may increase the N content and reduce the C/N ratio in the harvested straw biomass. In order to investigate the combined production of food, feed, and suitable feedstock (low C/N ratio) for lignocellulosic bioethanol production, a field experiment on cereal-legume intercropping was carried out in southern Sweden. In the experiment, autumn-sown cereals (wheat, triticale, and rye) were grown as monocrops with two different N fertilization levels (60 and 120 kg N ha−1) or intercropped with autumn-sown or spring-sown (undersown) clover-grass, autumn-sown or spring-sown (undersown) alfalfa, or autumn-sown faba bean. Total grain dry matter yield, cereal grain protein content, straw dry matter yield, and straw biomass C/N ratio were estimated. The results showed that the intercrops produced more straw biomass and had a lower C/N ratio, but produced less grain, than cereal monocrops. Among the cereals, rye showed significantly higher grain and straw yields, but lower grain protein content, in all treatments. Among the intercrop treatments, cereal with spring-sown clover-grass produced higher grain and straw yields and had a significantly lower C/N ratio (38:1) than the other treatments. Thus, cereal with spring-sown (undersown) clover-grass is a promising cropping system for lignocellulosic bioethanol production. [ABSTRACT FROM AUTHOR]

Despite the high prevalence of anemia and malnutrition in Myanmar, the relationship between anemia and body mass index (BMI) remains underexplored. We analyzed nationally representative data from the Myanmar Demographic and Health Survey (MDHS) 2015–2016 to explore the effects of BMI on hemoglobin levels and anemia among children (n = 2,937) and women (n = 9,262). Anemia was defined as hemoglobin levels < 11 g/dl for children and pregnant women, and < 12 g/dl for nonpregnant women, with BMI categories classified according to WHO guidelines. General linear models, Pearson's correlation, and logistic regression models were used in the analysis. Mean blood hemoglobin levels were 10.69 ± 1.30 g/dl for children and 11.96 ± 1.56 g/dl for women. Increased BMI was associated with increased hemoglobin levels in women (R = 0.142, p < 0.001). Logistic regression models predicted that severely underweight (COR, 1.26; CI: 1.02–1.57) and underweight women (COR, 1.17; CI: 1.02–1.34), pregnant women (AOR, 6.85; CI: 5.20–9.02), women with poorest wealth index (COR, 1.16; CI: 1.02–1.33), and women from rural regions (AOR, 1.13; CI: 1.01–1.26) and coastal zones (AOR, 1.65; CI: 1.40–1.95) were at higher risk of anemia. This study demonstrates a positive association between BMI and blood hemoglobin levels, with a lower risk of anemia in overweight and obese women. However, no associations were observed in children. Targeted nutritional interventions for underweight and pregnant women might be beneficial in mitigating anemia risk. [ABSTRACT FROM AUTHOR]

We provide new evidence on the economic and health impacts of government- mandated non-pharmaceutical interventions (NPIs) during the COVID-19 pandemic. Apart from labor force participation, unemployment, and hours worked, we provide novel results on work absence due to illness. We also examine the heterogeneity of these results by demographic and employment groups. We use recent innovations in the difference-in-differences methodology to capture the dynamic effects of these orders that were staggered in nature. Our findings show that states' social distancing measures increased unemployment and lowered labor market participation and hours worked. The adverse labor market effects were more pronounced for single parents and those working non-teleworkable jobs. We find some evidence that workers' health improved as absence from work due to illness significantly decreased, suggesting that NPIs protected many vulnerable workers. [ABSTRACT FROM AUTHOR]

Urban areas worldwide are increasing in size at an unprecedented rate, which is influencing local biodiversity. Most studies of urban biodiversity have focused on urban areas in developed countries, while knowledge from developing countries where much current urbanization is occurring is relatively scarce. Thus, in countries like Nepal that are undergoing significant urbanization (two-fold increase in 30 years), our understanding of the processes and impacts on biodiversity are extremely limited. In this study, we examine species richness, abundance, diversity and nesting habitat preferences of birds in Nepal’s capital Kathmandu in comparison to surrounding rural areas. Species richness, abundance and diversity were higher in rural sites than urban sites with a strong positive association with trees, which was much stronger in urban sites. Anthropogenic materials were incorporated into nests, and included plastics, wires and clothing. This behavior was observed primarily at urban sites, as was nesting on artificial substrates like power lines. Rural nesting preferences were positively associated with local vegetation and negatively with distances to buildings. Urban nesting preference were similar, but also increased with increasing distance to roads. Our results suggest that degrading urban green spaces may have severe impacts on urban avian diversity and facilitate colonization of generalist species, such as the House Crow (Corvus splendens), which was the most common species observed at urban sites. Our findings suggest that the planting and maintenance of trees throughout Kathmandu will help conserve current and future bird diversity. [ABSTRACT FROM AUTHOR]

Background and Aim: Continuous monocropping with high nitrogen (N) fertilizer input substantially increases greenhouse gas (GHG) emissions in maize-based agroecosystems in the North China Plain (NCP). Introducing soybeans as an intercrop with maize and partially substituting urea with manure might effectively decrease GHG emissions. The aim of this study was to quantify the synergistic effect of maize-soybean intercropping and manure on soil GHG emissions. Methods: A two-year field experiment with three cropping systems (maize monocrop, soybean monocrop, and maize-soybean intercrop) and four N treatments (control, urea, manure, and manure + urea) was carried out at Luancheng Agro-Ecosystem Experimental Station in the NCP. All N treatments, except the control, received 150 kg N ha−1season−1, either full dose as a basal application or two equal split applications. Results: Results showed that all treatments contributed as a net source of N2O and CO2 fluxes but acted as a net sink of CH4 fluxes. In both cropping seasons, intercrops had significantly lower N2O emissions compared to monocropping systems, with 38% and 14% less emissions than maize monocrops in 2018 and 2019, respectively. Additionally, maize monocrops had significantly higher soil CO2 emissions than other systems, while maize-soybean intercropping had 12% and 13% less CO2 emissions than maize monocrops in 2018 and 2019, respectively. Among fertilized treatments, manure-treated soils emit notably lower N2O fluxes compared to sole urea treatments. In this study, N2O and CO2 fluxes had a strong positive correlation with soil mineral N concentrations, soil temperature, and moisture content. Possibly due to more efficient N utilization, intercrop soils exhibited significantly lower NH4+ and NO3− concentrations, leading to reduced nitrification and denitrification in the system, resulting in lower N2O emissions from maize-soybean intercrops. Conclusion: Our findings indicate that intercropping maize and soybean reduces soil NH4+ and NO3– concentrations, as well as significantly decreasing soil N2O and CO2 emissions when compared to traditional maize monoculture. Therefore, due to its potential for reducing soil GHG emissions, maize-soybean intercropping can be regarded as an effective alternative cropping system to the prevailing maize-dominant monoculture to develop a sustainable agroecosystem in the NCP region. [ABSTRACT FROM AUTHOR]

Climate-induced increase in temperature and rainfall variability severely threaten the agricultural sector and food security in the Indian state of Odisha. Climate-smart agricultural (CSA) practices, such as crop rotation and integrated soil management, help farmers adapt to climate risk and contribute to a reduction in greenhouse gas (GHG) emissions. Therefore, this paper examines the impact of CSA practices on yield and income in vulnerable semi-arid districts of Odisha—Balangir, Kendrapara, and Mayurbhanj. We use primary survey data from 494 households collected in 2019–2020, using a multi-stage stratified sampling approach and structured questionnaire. Propensity score matching (PSM) and the two-stage least square method (2SLS) have been used to analyze the impact of CSA on income and productivity. Two instrument variables, namely distance to the extension office and percentage of adopters in a village, are used to control self-selection bias and endogeneity in our model. Both models show a positive and significant impact of the adoption of CSA on farmers’ productivity and income. The study sheds light on the significant contribution of CSA practices in fostering sustainable income growth amid environmental challenges. Overall, our results suggest that small and marginal farmers of Eastern India, a highly environmentally vulnerable area, can significantly improve their income and productivity by adopting CSA technology. Hence, policymakers should scale the adoption of CSA technology through effective extension programs. [ABSTRACT FROM AUTHOR]

Water resources assessment and management in ungauged basins are highly challenging but important task. It requires accurate simulation and estimation of streamflow characteristics. This study focuses on the simulation of rainfall-runoff processes in an ungauged Fusre River basin, a tributary of Seti River basin, Nepal using a semi-distributed hydrological model: Hydrologic Engineering Center-Hydrological Modeling System (HEC-HMS). The HEC-HMS model setup consists of a basin model, meteorological model, control specifications, and time series data. HEC-GeoHMS, an ArcGIS extension, enabled to prepare basin model parameters of the Seti river basin. Using daily streamflow data of Damauli hydrological station and precipitation data of 3 meteorological stations over the Seti basin, calibration and validation of the HEC-HMS model parameters were carried for 2007–2014 and 2015–2019 periods, respectively. Both continuous and event-based rainfall-runoff modeling were carried out to produce mean monthly and peak discharge values. Model performance was evaluated using values of NSE, RMSE, PBIAS, and R2 statistical parameters. After suitable values of these statistical parameters, the HEC-HMS model was applied for producing streamflow values in ungauged Fusre River basin. Flood-frequency analysis of simulated streamflow values projected a peak discharge of 457.3 m3/s and 534.6 m3/s for the 10- and 20-year return periods, respectively. [ABSTRACT FROM AUTHOR]

Rheumatoid arthritis (RA) is a chronic inflammatory disease characterized by an increased risk of cardiovascular events, due to the complex interplay between traditional and disease-related risk factors. Chronic inflammation and persistent disease activity are the key determinants of this risk, but despite great improvement in the disease management and prognosis, cardiovascular events are still the main cause of morbidity and mortality in RA cohorts1. In the last decades, the advent of new biological and targeted-synthetic DMARDs was accompanied by an improvement in disease activity control, but the role of each class of drugs on CVD risk is still a matter a debate. Since their approval for RA treatment, tumor necrosis factor alpha (TNFα) inhibitors have been widely investigated to better understand their effects on cardiovascular outcomes. The hypothesis that the reduction of chronic inflammation with any treatment may reduce the cardiovascular risk has been recently confuted by the direct comparison of TNFα-inhibitors and JAK inhibitors in patients with RA and coexisting risk factors for cardiovascular disease. The aim of this literature review is to add to the available evidence to analyze the relationship between TNFα-inhibitors and CVD risk in patients with RA and also provide some clinical scenarios to better explain the treatment dilemmas. In particular, while data on major cardiovascular events and thromboembolism seem consistent with an inflammation-mediated benefit with TNFα-inhibitors, there remain concerns about the use of this class of bDMARDs in patients with chronic heart failure. [ABSTRACT FROM AUTHOR]

Before becoming a cornerstone in the treatment of numerous immune-mediated diseases, mycophenolate mofetil (MMF) was first introduced as an immunosuppressive agent in transplant immunology and later received the attention of rheumatologists and clinicians involved in the management of autoimmune diseases. MMF is now a widespread immunosuppressive drug for the treatment of several conditions, including lupus nephritis, interstitial lung disease associated with systemic sclerosis, and anti-neutrophil cytoplasm antibody (ANCA)-associated vasculitis while being efficacious also as rescue therapy in various orphan diseases, including dermatomyositis and IgA-associated nephropathy. Similarly, case reports or series support a possible use of MMF in other rare autoimmune diseases. Beyond modulating lymphocyte activation, MMF acts on other immune and non-immune cells and these effects may explain the therapeutic profile of this medication. The effects of MMF are broadly characterized by the impact on the immune system and the antiproliferative and antifibrotic changes induced. In this latter case, mechanistic data on fibroblasts may in the future allow to reevaluate the use of MMF in selected patients with inflammatory arthritis or systemic sclerosis. Attention must be paid towards the possible occurrence of adverse events, such as gastrointestinal complaints and teratogenicity, while the risk of infections and cancer related to MMF needs to be further investigated. [ABSTRACT FROM AUTHOR]

A Gram-stain-positive, aerobic, and non-spore-forming bacterial strain, 20TX0166T, was isolated from a diseased onion bulb in Texas, USA. Upon testing its pathogenicity on onion bulb, it produced pathogenic response which makes it first species of pathogen belonging to the phylum actinobacteria detected in onion. Phylogenetic analysis of the 16S rRNA gene sequence revealed that the strain belonged to the genus Curtobacterium and was most similar to Curtobacterium flaccumfaciens LMG 3645T (100%), C. pusillum DSM 20527T (99.5%), and C. oceanosedimentum ATCC 31317T (99.5%). The estimated genome size of the novel species was 4.0 Mbp with a G + C content of 70.8%. The orthologous ANI (orthoANIu), ANI based on blast (ANIb), and dDDH values between the novel strain and the closest relative, C. flaccumfaciens LMG 3645T, were 95.7%, 95.4%, and 63.3%, respectively. These values were below the recommended species cut-off threshold of 96% (ANI) and 70% (dDDH), suggesting the strain may be a novel species. Physiologic and phenotypic characters of this novel strain were also unique when compared with the closely related species. The major cellular fatty acids of this strain were anteiso-C15:0 and anteiso-C17:0. Using a polyphasic approach based on phenotypic and genotypic analyses, strain 20TX0166T represents a novel species of the genus Curtobacterium, and the name Curtobacterium allii sp. nov. is proposed. The type strain is 20TX0166T (= LMG 32517T = CIP112023T = NCIMB 15427T). [ABSTRACT FROM AUTHOR]

Predicting upper stem diameters and individual tree volumes is important for product quantification and can provide important information for the sustainable management of forests of important commercial tree species (Shorea robusta) in Nepal. The aim of this study was to develop a taper equation for S. robusta. Fifty-four trees were selected and felled in the southern low land of Nepal. A mixed effect modelling approach was used to evaluate 17 different taper functions. 'Leave-one-out cross-validation' was used to validate the fitted taper functions. The variable exponent taper function best fitted our data and described more than 99% of the variation in upper stem diameters. Results also showed significant effects of stand density on tree taper. Individual tree volume prediction using the local volume model developed in this study was more accurate compared to the volume predicted through the taper function and existing volume model. The taper function developed in this study provides the benefit of predicting upper stem diameter and can be used for predicting volume to any merchantable height of individual trees. It will have implications in estimates of volume, biomass, and carbon and thus may be a potential supporting tool in carbon trade and revenue generation. [ABSTRACT FROM AUTHOR]

Photodynamic therapy (PDT) research would benefit from an automated, low-cost, and easy-to-use cell culture light treatment setup capable of illuminating multiple well replicates within standard multiwell plate formats. We present an LED-array suitable for performing high-throughput cell culture PDT experiments. The setup features a water-cooling loop to keep the LED-array temperature nearly constant, thus stabilizing the output power and spectrum. The setup also features two custom-made actuator arms, in combination with a pulse-width-modulation (PWM) technique, to achieve programmable and automatic light exposures for PDT. The setup operates at ~ 690 nm (676–702 nm, spectral output full-width half-maximum) and the array module can be readily adapted to other LED wavelengths. This system provides an illumination field with adjustable irradiance up to 400 mW/cm2 with relatively high spectral and power stability comparing with previously reported LED-based setups. The light doses provided by the LED array were validated with comparison to traditional laser PDT. This open-source illumination platform (including the detailed technical description, fabrication protocols, and parts list provided here) helps to make custom light sources more accessible and of practical use for photomedicine research. [ABSTRACT FROM AUTHOR]

Purpose: The key environmentally beneficial process that substantially removes reactive nitrogen from biosphere is a complete denitrification. The science of measuring and constraining nitrous oxide (N2O) emissions has advanced significantly; however, despite several attempts, in situ dinitrogen (N2) measurement is still a great challenge and is poorly understood due to the high atmospheric N2 background. This study aimed at estimating field-scale inferred N2 emissions using data of field N2O emissions and laboratory-measured N2O/(N2O + N2) ratios and correlating those emissions with the soil-environmental factors. Materials and methods: Closed static chamber and He/O2 direct measurement methods were used at field and laboratory scale, respectively. For each treatment (varying N fertilizer rates), on each sampling date, N2O and N2 emissions were measured at laboratory and N2O emissions at field-scale, allowing the calculation of field-scale inferred N2 emissions. Results: The results demonstrate that field-scale inferred cumulative N2 emissions were 1.35, 1.48 and 1.60 times greater than laboratory-measured cumulative N2 emissions in low nitrogen level (LNL), medium nitrogen level (MNL) and high nitrogen level (HNL) treatments, respectively. This suggests that estimating N2 emissions at the field-scale in agricultural soil could give more insight on N cycling processes. Moreover, N fertilizer application rates increased linearly both field and laboratory cumulative N2O and N2 emissions. Both positive and negative relationships between soil-environmental parameters and N2O, N2 and their N2O/(N2O + N2) ratios at field and laboratory-based indicate their heterogeneous roles in N2O formation and reduction processes. Conclusion: The results provide complementary insights into field-scale N2 emissions in agricultural soil and help in closing the knowledge gap in the N balance. Linear relationships between the emissions (N2O and N2) and N fertilizer rates observed suggest that climate change mitigation options could be achieved by optimizing the N fertilization rates since N2O and N2 emissions are enhanced by increasing N inputs. As our results present the field-scale inferred N2 emissions, there is still a need to design a robust methodological approach that will enable researchers to directly quantify field N2 emissions. [ABSTRACT FROM AUTHOR]

A systematic study on the biological effects of simulated microgravity (sµg) on human pluripotent stem cells (hPSC) is still lacking. Here, we used a fast-rotating 2-D clinostat to investigate the sµg effect on proliferation, self-renewal, and cell cycle regulation of hPSCs. We observed significant upregulation of protein translation of pluripotent transcription factors in hPSC cultured in sµg compared to cells cultured in 1g conditions. In addition to a significant increase in expression of telomere elongation genes. Differentiation experiments showed that hPSC cultured in sµg condition were less susceptible to differentiation compared to cells in 1g conditions. These results suggest that sµg enhances hPSC self-renewal. Our study revealed that sµg enhanced the cell proliferation of hPSCs by regulating the expression of cell cycle-associated kinases. RNA-seq analysis indicated that in sµg condition the expression of differentiation and development pathways are downregulated, while multiple components of the ubiquitin proteasome system are upregulated, contributing to an enhanced self-renewal of hPSCs. These effects of sµg were not replicated in human fibroblasts. Taken together, our results highlight pathways and mechanisms in hPSCs vulnerable to microgravity that imposes significant impacts on human health and performance, physiology, and cellular and molecular processes. [ABSTRACT FROM AUTHOR]

Corynespora cassiicola isolates display morphological, pathogenic, and ecological diversity, inducing target spot-like diseases in more than 500 hosts worldwide, including tomato and cucumber. Nevertheless, there is a scarce number of studies about the genetic variability of Corynespora isolates in the New World. Here, we characterized a collection of 58 Corynespora isolates from tomatoes and distinct hosts in Brazil and Florida (USA). All isolates were identified as C. cassiicola according to the sequencing information of the internal transcribed spacer (ITS) region of 18S–28S nuclear ribosomal DNA as well as the translation elongation factor 1-alpha (tef-1α) and β-tubulin (tub2) genes. However, intraspecific resolution was observed in phylogenetic analyses according to the geographical and host origin of the isolates. The β-tubulin (tub2) haplotype network was in agreement with phylogenetic analyses, revealing a polyphyletic structure with three well-defined phylogenetic lineages. The concatenated trees (encompassing all three genomic regions) showed superior intraspecific resolution than the individual phylogenetic trees. Thirteen selected C. cassiicola isolates (representing all three phylogenetic lineages) displayed variability in colony morphology (color, texture, growth rate, and shape) and in conidial morphometrics. Three selected C. cassiicola isolates confirmed their pathogenicity to the original hosts and to other plant species. Novel natural and experimental host-pathogen interactions were identified, including cabbage, Commelina benghalensis, eggplant, Eruca sativa, Hibiscus sabdariffa, and melon. The diversity of C. cassiicola isolates indicates that these phylogenetic lineages may represent a complex of closely related species with distinct patterns of host and cultivar-specificity. [ABSTRACT FROM AUTHOR]

Serine Incorporator 5 (SERINC5), a cellular multipass transmembrane protein that is involved in sphingolipid and phosphatydilserine biogenesis, potently restricts a number of retroviruses, including Human Immunodeficiency Virus (HIV). SERINC5 is incorporated in the budding virions leading to the inhibition of virus infectivity. In turn, retroviruses, including HIV, encode factors that counteract the antiviral effect of SERINC5. While SERINC5 has been well studied in retroviruses, little is known about its role in other viral families. Due to the paucity of information regarding host factors targeting Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), we evaluated the effect of SERINC proteins on SARS-CoV-2 infection. Here, we show SERINC5 inhibits SARS-CoV-2 entry by blocking virus-cell fusion, and SARS-CoV-2 ORF7a counteracts the antiviral effect of SERINC5 by blocking the incorporation of over expressed SERINC5 in budding virions. SERINC5, is a cellular multipass transmembrane protein involved in sphingolipid and phosphatydilserine biogenesis and a known retroviral restriction factor. Here, Timilsina et al. show that SERINC5 is a host restriction factor for SARS-CoV-2 that prevents viral fusion during entry. Further they show that viral ORF7a counteracts SERINC5 anti-viral activity by blocking its incorporation into progeny virions. [ABSTRACT FROM AUTHOR]

The major limitations of DNA-targeting chemotherapy drugs include life-threatening toxicity, acquired resistance and occurrence of secondary cancers. Here, we report a small molecule, Carbazole Blue (CB), that binds to DNA and inhibits cancer growth and metastasis by targeting DNA-related processes that tumor cells use but not the normal cells. We show that CB inhibits the expression of pro-tumorigenic genes that promote unchecked replication and aberrant DNA repair that cancer cells get addicted to survive. In contrast to chemotherapy drugs, systemic delivery of CB suppressed breast cancer growth and metastasis with no toxicity in pre-clinical mouse models. Using PDX and ex vivo explants from estrogen receptor (ER) positive, ER mutant and TNBC patients, we further demonstrated that CB effectively blocks therapy-sensitive and therapy-resistant breast cancer growth without affecting normal breast tissue. Our data provide a strong rationale to develop CB as a viable therapeutic for treating breast cancers. A report of the small molecule Carbazole Blue demonstrates inhibition of breast cancer growth and metastasis without adverse effects on normal tissue. [ABSTRACT FROM AUTHOR]

Purpose: To describe electrocardiographic vector patterns during early VF transition (Wiggers stage 1). Methods: In 100 electrophysiology studies with VF induction, the first 3 beats of VF were analyzed in lead I for left/right axis (LA/RA), V1 for left/right bundle (LB/RB), and aVF for superior/inferior axis (SA/IA). Correlation with demographic/clinical factors was performed using regression analyses and mixed effect modeling. Results: VF initiated more likely with LA than RA (P < 0.001) and LB than RB (P = 0.04) suggesting original wavebreak in the right ventricle. The 3-dimensional morphology changed in 69% of VF during the first 3 beats, with predominant increase in RB, suggesting a transition of QRS-originating vector to septum/left ventricle. Conservation of morphology (31%) was favored by initial RB (P = 0.002) and LA morphology (P = 0.01). Initiation of VF with LA vs RA was more likely in African-Americans (P = 0.016) and increasing age (P = 0.032). Ischemic cardiomyopathy favored VF initiation with RB 6.7-fold (P = 0.025), possibly linking LV myocardial scar to initial VF wavebreak location. Male gender and ischemic cardiomyopathy prolonged time-to-loss of predominant vector by 119% (P = 0.002) and 71% (P = 0.017), respectively, suggesting more preserved anatomic/functional reentry. Conclusion: The predominant QRS vectors during early Wiggers stage 1 VF are not random and suggest an initial wavebreak more commonly in the right ventricle, followed by a transitional shift to the septum/left ventricle. Ethnicity, male gender, age, and co-morbidities result in directional preservation of initiating VF vectors possibly due to myocardial mass/fibrosis. Findings may allow new treatment/ablation approaches. [ABSTRACT FROM AUTHOR]

The enzymatic activities and ratios are critical indicators for organic matter decomposition and provide potentially positive feedback to carbon (C) loss under global warming. For agricultural soils under climate change, the effect of long-term warming on the activities of oxidases and hydrolases targeting C, nitrogen (N) and phosphorus (P) and their ratios is unclear, as well as whether and to what extend the response is modulated by long-term fertilization. A 9-year field experiment in the North China Plain, including an untreated control, warming, N fertilization, and combined (WN) treatment plots, compared the factorial effect of warming and fertilization. Long-term warming interacted with fertilization to stimulate the highest activities of C, N, and P hydrolases. Activities of C and P hydrolase increased from 8 to 69% by N fertilization, 9 to 53% by warming, and 28 to 130% by WN treatment compared to control, whereas the activities of oxidase increased from 4 to 16% in the WN soils. Both the warming and the WN treatments significantly increased the enzymatic C:N ratio from 0.06 to 0.16 and the vector length from 0.04 to 0.12 compared to the control soil, indicating higher energy and resource limitation for the soil microorganisms. Compared to WN, the warming induced similar ratio of oxidase to C hydrolase, showing a comparable ability of different microbial communities to utilize lignin substrates. The relationship analyses showed mineralization of organic N to mediate the decomposition of lignin and enzyme ratio in the long-term warming soil, while N and P hydrolases cooperatively benefited to induce more oxidase productions in the soil subject to both warming and N fertilization. We conclude that coupled resource limitations induced microbial acclimation to long-term warming in the agricultural soils experiencing high N fertilizer inputs. [ABSTRACT FROM AUTHOR]

Seven days of extreme rainfall during September 2013 produced more than 1100 debris flows in the Colorado Front Range, about 78% of which occurred on south-facing slopes (SFS). Previously published soil moisture (volumetric water content) observations suggest that SFS were wetter than north-facing slopes (NFS) during the event, which contrasts with soil moisture patterns observed during normal conditions. Various causes have been hypothesized for the preferential saturation of SFS, but those hypotheses remain largely untested. Here, we analyze the soil moisture patterns using additional soil moisture observations, determine the hydrologic processes controlling the preferential saturation of SFS, and evaluate the importance of soil moisture in predicting the debris flow initiation sites. Soil moisture patterns are simulated using the Equilibrium Moisture from Topography, Vegetation, and Soil (EMT + VS) model. Five hypotheses are tested that may have influenced the soil moisture reversal including higher rainfall rates, lower interception rates, lower saturated water content, thinner soils, and reduced deep drainage on SFS. The EMT + VS model is coupled with an infinite slope stability model to produce factor of safety maps. The hypotheses are tested by comparing the modeled soil moisture to soil moisture observations and the debris flow initiation sites. The results suggest that differences in interception and deep drainage between SFS and NFS were primarily responsible for producing wetter SFS, but the soil moisture pattern likely played a smaller role than vegetation and slope in determining where debris flows initiated. The final model predicts instability at approximately 72% of the observed debris flow initiation sites. [ABSTRACT FROM AUTHOR]

Purpose: Nitrous oxide (N2O) measured simultaneously with di-nitrogen (N2) emissions from soils are greatly uncertain due to large temporal and spatial variations. This study aims to report N2O, N2, N2/N2O, 15N-N2O, wheat-maize annual grain yields, and yield-scaled N2O and N2O plus N2 emissions on the responses to different nitrogen (N) fertilizer rates in a winter wheat-summer maize cropping system. Furthermore, this study also seeks to determine controlling factors for N2O, N2, and N2/N2O emissions and significantly investigate the relationship between the soil-climate measured factors and 15N-N2O. Materials and method: Three N inputs and control treatments, 0, CK; 200, LN; 400, MN; and 600, HN kg N ha−1 year−1 were set since 1998. Direct measurement method has been used to quantify N2O and N2 emissions. Results: Our results indicated that the effects of long-term N fertilization significantly increased N2O and N2 and also reduced N2/N2O emission ratios as described by exponential functions. Using structural equation modeling (SEM), NH4+, WFPS, NO3-, and DOC were revealed to be main controlling factors for N2O, while N2 by DOC, NO3-, WFPS, and temperature finally N2/N2O was positively related to temperature. Furthermore, the 15N-N2O was positively related to N2/N2O ratios, indicating that denitrification is the dominant process at the study site. The yield-scaled N2O emissions followed the order HM>MN>LN>CK, and they were 1.56, 1.47, and 1.07 times greater than CK, respectively. Total yield-scaled N2O plus N2 were in the order of CK>HN>MN>LN. Conclusion: N fertilization has shown strong impact not only on N2O, N2, and N2/N2O emissions but also on yield-scaled N2O and N2O plus N2 emissions. High agronomic nitrogen use efficiency (NUE), low yield-scaled N2O emissions, and low cumulative N2O plus N2 emissions were observed at 200-LN treatment, suggesting this rate to be an optimum and sustainable agricultural management practice with no significant crop yield reduction as compared to the current farmers' practice of 400 kg N ha−1 year−1. [ABSTRACT FROM AUTHOR]

The unique geography and fragile geological condition have made Nepal more prone to various types of disasters. The 2015 Gorkha Earthquake had a serious effect on one-third of the population of Nepal. Disaster comes with both opportunities and consequences. It depends on how a country and affected people deal with the situations. After the 2015 Gorkha Earthquake, many people especially those from the affected districts were assumed to be sensitized regarding the consequences of Earthquake disaster. In general, the disaster affected people have certainly gained know-how of the disaster to some extent even through experiences. But, the people who are less affected by the 2015 Gorkha Earthquake are yet to be aware of its major consequences. Hence, this research aims to understand the perception of people who were not severely affected by the 2015 Gorkha Earthquake but they are at high risk of future earthquake events. This research evaluated whether such people are aware of the major disaster consequences or not. Likewise, it also evaluated whether they are working on the risk reduction and disaster preparedness plans to minimize human and property loss in the future or not. So, this research was carried out in the rapidly urbanizing Pokhara City situated in the western part of Nepal which is at high risk of various types of disasters including earthquakes. The research was focused on understanding the perception of risk perceived by the people of Pokhara City in the aftermath of the 2015 Gorkha Earthquake. A household survey was conducted that involved the individual interview of a total of 152 participants from randomly selected houses. Various questions related to Disaster Risk Reduction (DRR) issues including readiness and mitigating behaviour of people, disaster risk adaptation and perceptions were asked. Histogram analysis, distribution analysis, bivariate correlations and independent sample t-tests were conducted to examine the relationship between people in disaster education-related programs and how they are following key DRR issues related to dependent (criterion) variables. A series of independent sample t-tests were conducted to examine the effects of age, gender, and disaster events on the dependent variables. Likewise, to evaluate the coherency of the data provided by the respondents, the Partial Credit Model (PCM) was used. Validity, reliability and unidimensionality of the scale were further evaluated by using PCM. The findings of this research show that the people residing in such a rapidly urbanizing Pokhara City are completely unaware of the major consequences of various types of disasters. Almost 60% of the respondents even well-educated conceived that the disaster is merely the act of God that is beyond our imagination to control and mitigate. This easily concludes that in Pokhara City, the local community, technicians and even policymakers are unaware of the proper disaster management plans and policies. Pokhara city lacks awareness of upcoming disasters and it is a serious drawback that can create too many issues in sustainable development practices in the city. [ABSTRACT FROM AUTHOR]

The conversion of natural grassland to semi-natural or artificial ecosystems is a large-scale land-use change (LUC) commonly occurring to saline–alkaline land. Conversion of natural to artificial ecosystems, with addition of anthropogenic nitrogen (N) fertilizer, influences N availability in the soil that may result in higher N2O emission along with depletion of 15N, while converting from natural to semi-natural the influence may be small. So, this study assesses the impact of LUC on N2O emission and 15N in N2O emitted from naturally occurring saline–alkaline soil when changing from natural grassland (Phragmites australis) to semi-natural [Tamarix chinensis (Tamarix)] and to cropland (Gossypium spp.). The grassland and Tamarix ecosystems were not subject to any management practice, while the cropland received fertilizer and irrigation. Overall, median N2O flux was significantly different among the ecosystems with the highest from the cropland (25.3 N2O-N µg m−2 h−1), intermediate (8.2 N2O-N µg m−2 h−1) from the Tamarix and the lowest (4.0 N2O-N µg m−2 h−1) from the grassland ecosystem. The 15N isotopic signatures in N2O emitted from the soil were also significantly affected by the LUC with more depleted from cropland (− 25.3 ‰) and less depleted from grassland (− 0.18 ‰). Our results suggested that the conversion of native saline–alkaline grassland with low N to Tamarix or cropland is likely to result in increased soil N2O emission and also contributes significantly to the depletion of the 15N in atmospheric N2O, and the contribution of anthropogenic N addition was found more significant than any other processes. [ABSTRACT FROM AUTHOR]

Climate change is posing risks for human and natural systems, and one of the most important questions faced by policy makers is to reduce such risks and impacts through adaption and mitigation actions. As the country with the highest CO2 emissions, China is facing unprecedented challenges: climate change, energy structure transformation and socio-economic development all pose complex dilemmas for policy makers. The Chinese government has introduced energy policy and emission reduction measures to achieve its climate change mitigation targets. However, overlap between these measures may generate great uncertainty about their performance. By using a multi-regional computable general equilibrium (CGE) model, this study examines the economic impacts of implementing a nationwide carbon market and a renewable energy subsidy in the power sector in China. The study finds that a renewable energy policy would increase the economic cost of reducing greenhouse gas emissions and lead to a lower carbon price level if a carbon pricing mechanism were in place. However, the combination of the two policies indeed shows advantages in achieving emission reduction targets and renewable energy promotion targets. While an emission trading scheme is necessary for reducing emissions effectively, a renewable energy policy could promote the large-scale use of renewable energy in the electricity sector. In addition, the adoption of a renewable energy policy could yield substantial inter-regional capital inflow to central and western regions. This would improve low-carbon investment in less developed regions, and thus an appropriately designed policy mix would be sensible. [ABSTRACT FROM AUTHOR]

Anisotropic magnetoelectric (ME) responses in bi-layered composites consisting of ferrites and polycrystalline/crystalline piezoelectrics were investigated by changing the applied DC magnetic field orientations, and origins for such effects were clarified by the competition between the dynamic piezomagnetic strain and the dynamic electro-mechanical strain from individual piezomagnetic/piezoelectric subsystems. Experimental results showed that maximum resonance ME voltage coefficient (MEVC) of 133.8.3 V/cm Oe under HDC = 23Oe can be obtained at θ = 0° for PMN-PT/NZFO laminate while one reached 115.3 V/cm Oe at higher field of 98Oe for θ = 75°. As rotation angle θ is varied from 0° to 360°, the HDC-orientation-dependent ME couplings were mainly determined by the dynamic piezomagnetic strain, and the dynamic piezoelectric coefficient versus θ profile can essentially track the MEVC versus θ profile. In addition, we found that the MEVC for PMN-PT/NZFO laminate is approximately twice as high as that of the PZT-8/NZFO laminate due to the stronger electro-mechanical strain of PMN-PT plates, Solid evidence was proved for the HDC-orientation-dependent ME coupling of the rectangle composite that is free of the influences of anisotropic crystalline orientation in PMN-PT plate. These findings open up a research pathway to move forward in this field and provide potential applications in vector angle sensors. [ABSTRACT FROM AUTHOR]

Electric-field-controlled resonance tuning in tri-layer magnetoelectric laminate consisting of Ni–Zn ferrites (with/without Sm-doped) and PZT plates has been performed and systemically characterized. By applying the DC voltage on PZT plate, modulus of the sample will change in ferrite which is caused by mediated pre-stress from PZT plate, resulting in a resonance shifting without a sacrifice of MEVC decay. For the purpose of tuning range improvement, light doping of samarium was employed in spinel nickel–zinc ferrites to modify frequency responses properties. Experimental results showed that a nearly linear variation of resonance frequency with applied voltage varied from 0 to 6 kV/cm was obtained. Consequently, the resonance frequency can be precisely controlled by the pre-stress generated from DC electric field. In addition, the tunability of resonance frequency exhibits an approximately 3.68 times enhancement by samarium doping in the Ni–Zn ferrites. The pathway for resonance frequency tunable realization by applied DC voltage provides an accurate tuning method for the tunable resonator, which can be deployed extensively in signal generators, phase shifters and other electronic devices. [ABSTRACT FROM AUTHOR]

The use of cereal microgreens is increasing because of increased consumer's interest in healthier products. Chlorophyll (Chl) and Carotenoids (Car) are suggested to correlate with health promoting components like phenolics and antioxidant potential of the plant-part. They also play role against clinical conditions like thalassemia and hemolytic anemia and reduce the risk of some chronic diseases, such as cancer, cardiovascular diseases, skin diseases and age-related eye diseases. This study was carried out for the comprehensive profiling of Chl and Car in wheat (Triticum aestivum L.), and barley (Hordeum vulgare L.) micro-greens between 7 and 16 days on dry basis. Chl and Car content strongly correlated with the number of days of growth. Significantly high correlations existed among Chl a, Chl b, total Chl and total Car with concomitant Chl a/b and Chl/Car ratios. The peaks for the rate of accumulation of pigments were between 7–10 days on wheat and 10–13 days on barley. The maximum content of Chl and Car were 616.63 ± 18.45 mg/100 g DM and 54.80 ± 1.72 mg/100 g DM on day 16 and statistically not significant to variety of grain. The Chl level was slightly lower than Chl rich vegetables like kale and comparable to spinach and Car level was comparable to carrots, which is higher than most of the daily consumable fruits and vegetables. Further cell-based or in vivo studies of cereal microgreens could be considered to draw more valuable information related to human health. [ABSTRACT FROM AUTHOR]

With the explosive growth of global energy demand coupled with effects of climate change, there is a significant shift towards green energy generation in recent years. Of the various renewable energy resources available, micro-hydro-power and pico-hydro-power remain very popular in both developed and developing countries. Since 2006, significant growth has spurted in the use of artificial free-surface vortices to generate low and ultra-low-head hydropower following the development of the so-called gravitational water vortex hydropower plant. The technology works on the principle of harnessing hydroelectric power from the high angular velocity experienced in the core of a whirlpool generated in a vortex chamber. In this article, a state-of-the-art review is undertaken on the vortex hydropower technology including a historical review of the technology, the underlying hydraulic principles of such devices, overview of research and technologies that have been deployed to date together with an evaluation of their performance and key findings. Currently, there are 19-22 known live vortex hydropower technologies operating internationally with key academic and commercial research activity in Europe and Asia. The average efficiency from these sites was found to be in the region of 53% which is lower than conventional propeller turbines but higher than waterwheel systems. It was found that the vortex plant, due to its ability to sustain relatively high efficiencies at low heads and small to medium flow rates, addresses a gap in the current turbine application chart. Its key advantage lies in the high-power densities produced compared with conventional technologies. The system also demonstrates potential to be able to function as a fish passage; however, stronger validation is required to prove this for a range of turbine systems. Finally, the authors propose a number of areas that should be investigated that should provide immediate improvements to the turbine in terms of performance. [ABSTRACT FROM AUTHOR]

Despite the declining trends in total energy consumption, greenhouse gas emissions, energy intensity, and emission intensity over the past two decades, Romania still emits more greenhouse gas per unit of output than many other members of the European Union. The country is looking for further greening of its energy supply system to achieve the clean energy and climate change mitigation goals included in the European Union's 2030 target and 2050 Roadmap. Using an energy supply optimization model, TIMES, this study develops energy supply mixes for Romania under a baseline scenario that satisfies the European Union's current energy and climate targets for 2020, a green scenario that satisfies the European Union's 2030 energy and climate targets, and a super green scenario that satisfies the European Union's prospective 2050 energy road map. The study finds that although Romania could achieve the green scenario at a moderate cost, it would be challenging and costly to achieve the super green scenario. [ABSTRACT FROM AUTHOR]

Research policy makers, funding agencies, universities and government organizations evaluate research output or impact based on the traditional citation count, peer review, h-index and journal impact factors. These impact measures also known as bibliometric indicators are limited to the academic community and cannot provide the broad perspective of research impact in public, government or business. The understanding that scholarly impact outside scientific and academic sphere has given rise to an area of scientometrics called alternative metrics or 'altmetrics.' Moreover, researchers in this area incline to center around gauging scientific activity via social media, namely Twitter. However, these count-based measurements of impact are sensitive to gaming as they lack concrete references to the primary source. In this work, we expand a conventional citation graph to a heterogeneous graph of publications, scientists, venues, organizations based on more reliable social media sources such as mainstream news and weblogs. Our method is composed of two components: the first one is combining the bibliometric data with social media data like blogs and mainstream news. The second component investigates how standard graph-based metrics can be applied to a heterogeneous graph to predict the academic impact. Our result showed moderate correlations and positive associations between the computed graph-based metrics with academic impact and also reasonably predict the academic impact of researchers. [ABSTRACT FROM AUTHOR]

Phagocytic clearance of apoptotic germ cells by Sertoli cells is vital for germ cell development and differentiation. Here, using a tissue-specific miRNA transgenic mouse model, we show that interaction between miR-471-5p and autophagy member proteins regulates clearance of apoptotic germ cells via LC3-associated phagocytosis (LAP). Transgenic mice expressing miR-471-5p in Sertoli cells show increased germ cell apoptosis and compromised male fertility. Those effects are due to defective engulfment and impaired LAP-mediated clearance of apoptotic germ cells as miR-471-5p transgenic mice show lower levels of Dock180, LC3, Atg12, Becn1, Rab5 and Rubicon in Sertoli cells. Our results reveal that Dock180 interacts with autophagy member proteins to constitute a functional LC3-dependent phagocytic complex. We find that androgen regulates Sertoli cell phagocytosis by controlling expression of miR-471-5p and its target proteins. These findings suggest that recruitment of autophagy machinery is essential for efficient clearance of apoptotic germ cells by Sertoli cells using LAP. [ABSTRACT FROM AUTHOR]

Citrus surveys were conducted at high (>700 m), medium (300-600 m) and low (<200 m) altitudes in Tanzania in 2014/15. Adults and nymphs of Trioza erytreae (del Guercio) were abundant in the highlands and less abundant at medium altitudes. Unexpectedly, adults and nymphs of Diaphorina citri Kuwayama, the Asian citrus psyllid, were found and collected at medium altitudes, around Morogoro. No psyllids were observed at low altitudes. Severe huanglongbing symptoms and tree decline were evident at high altitudes, while mild and few symptoms were observed at intermediate and low altitudes, respectively. DNA was extracted from leaf and psyllid samples and subjected to conventional PCR (cPCR) with seven different primer sets and RT qPCR with two primer sets. cPCR bands were sequenced and subjected to phylogenetic analysis. Candidatus Liberibacter africanus (Laf) was detected in highland leaf and T. erytreae samples from high and medium altitudes by all methods. Sequences from leaves and psyllids were similar to those from South Africa. Candidatus Liberibacter asiaticus (Las) was detected by qPCR in medium altitude leaf samples, but cross-reaction with Laf was likely because presence of Las was not confirmed by cPCR and sequencing. Neither Laf nor Las were detected in D. citri samples. This is the first reported occurrence of D. citri in Africa. Predictions were made of the potential distribution of D. citri and Las in Africa and along the Mediterranean coast using the correlative models MAXENT and Multi-Model Framework. Additional surveys at medium and low altitudes and quarantine measures are recommended. [ABSTRACT FROM AUTHOR]

The term mechanoluminescence (ML) refers to the light emission from various organic and inorganic materials in response to mechanical stimuli such as friction, tension, fracture, and compression. Recently, this interesting luminescence phenomenon has enabled us to explore various advanced sensor applications. In this review article, the overall ML mechanism that arises under different experimental conditions is discussed thoroughly. The recent research achievements of state-of-the-art sensors based on ML microparticles such as SrAlO:Eu,Dy (SAO), ZnS:Cu, and ZnS:Mn are then reviewed. As a new functional smart material, SAO ML microparticles can be used in possible substitutive and substantial methods for structural health monitoring (SHM) systems, owing to their relative simplicity but effectiveness in detecting arrested or propagating crack tips, and in assessing the in-situ structural state by means of fracture parameters such as stress intensity factors. The applicability of ML paint and thin films on various types of ceramics and concretes as fracture sensors is reviewed. The research on sensors based on different ML microparticles such as noncontact torque sensors based on ZnS:Cu, flexible pressure sensors and impact sensors using ZnS:Mn is also reviewed in this article. Potential applications using ML microparticles are also briefly discussed. [ABSTRACT FROM AUTHOR]

Background: The 2015.4.25 Gorkha earthquake affected about eight million people in Nepal. Most injuries and loss of life were due to building collapse and damage. This work aims to investigate the topographical and geological effects on the severe damage caused by this earthquake. Findings: In one-week field investigation in the earthquake-affected areas, several severely damaged areas with different topographic and geological features were surveyed, as well as the site of Kaligandaki River landslide dam failure. Some general tendency related to the building damage and landslide dam failure was obtained. Conclusions: Through the field investigation, it was found that geological and geomorphological characteristics of a site, combined with the structure feature of the building, such as the short column effect, amplified the seismic vibration and caused severe building collapse and damages, i.e., 1) For buildings on flat area consisting of lacustrine deposit or diluvial deposit, resonance effect might be the main reason, while for the buildings on the top of hills or narrow ridges, topographic effect and sometimes, short column effect should take the main responsibility; 2) For buildings located on the gentle slopes or landslides, the settlement in the infill side caused by the strong seismic vibration can be the main reason; 3) Besides of the building failure on lacustrine deposit, failure patterns in three types of topographic and geological features, i.e., narrow ridges formed by landslides, diluvial deposits and alluvial fans, and landslides, were proposed as the possible mechanism of the building damage caused by the earthquake. For landslide dam failure, it was found that landslide dam could easily breach or collapse, when the landslide-dam-deposits were fine. [ABSTRACT FROM AUTHOR]

Visceral leishmaniasis (VL) is a major vector-borne disease caused by Leishmania donovani, after replication of the parasites in macrophages, mononuclear phagocytic system. VL is endemic in 12 districts of central and eastern Terai lowlands of Nepal bordering North Bihar, India with an estimated 8 million population at risk. In addition, VL endemicity is also extending to new endemic regions like Dharan from its classical rural foci. Hence, we aimed to detect the evidence of Leishmania donovani infection in the blood samples received from blood donors of Sunsari district, Dharan, (eastern Nepal), a region endemic for human VL. Sera from 507 asymptomatic blood donors were subjected to serological screening for anti- Leishmania donovani antibodies. Direct agglutination test (DAT) was performed on the sera. Out of 507 donors, majority (78.50 %) were male. Among the donors, 472 (93.10 %) belonged to age group 18-45 years where as 35 (6.90 %) to age group >45 years. Circulating anti- Leishmania antibodies were detected in 5 (1 %) out of 507 healthy, Human Immunodeficiency Virus types 1 and 2 (HIV 1and 2), Hepatitis B Surface Antigen (HBsAg), anti- Hepatitis C Virus (anti-HCV)-negative, and Syphillis non-reactive donors. All the seropositive cases were male and belonged to the age group 18-45 years. The result suggests that there is an immediate need of screening asymptomatic blood donors for leishmania seropositivity especially in endemic areas. [ABSTRACT FROM AUTHOR]

Global energy demand is increasing rapidly and due to intensive consumption of different forms of fuels, there are increasing concerns over the reduction in readily available conventional energy resources. Because of the deleterious atmospheric effects of fossil fuels and the uncertainties of future energy supplies, there is a surge of interest to find environmentally friendly alternative energy sources. Hydrogen (H) has attracted worldwide attention as a secondary energy carrier, since it is the lightest carbon-neutral fuel rich in energy per unit mass and easy to store. Several methods and technologies have been developed for H production, but none of them are able to replace the traditional combustion fuel used in automobiles so far. Extensively modified and renovated methods and technologies are required to introduce H as an alternative efficient, clean, and cost-effective future fuel. Among several emerging renewable energy technologies, photobiological H production by oxygenic photosynthetic microbes such as green algae and cyanobacteria or by artificial photosynthesis has attracted significant interest. In this short review, we summarize the recent progress and challenges in H-based energy production by means of biological and artificial photosynthesis routes. [ABSTRACT FROM AUTHOR]

There are few field-based, empirical studies quantifying the effect of invasive trees and palms and maintenance-related carbon emissions on changes in urban forest carbon stocks. We estimated carbon (C) stock changes and tree maintenance-related C emissions in a subtropical urban forest by re-measuring a subsample of residential permanent plots during 2009 and 2011, using regional allometric biomass equations, and surveying residential homeowners near Orlando, FL, USA. The effect of native, non-native, invasive tree species and palms on C stocks and sequestration was also quantified. Findings show 17.8 tC/ha in stocks and 1.2 tC/ha/year of net sequestration. The most important species both by frequency of C stocks and sequestration were Quercus laurifolia Michx. and Quercus virginiana Mill., accounting for 20 % of all the trees measured; 60 % of carbon stocks and over 75 % of net C sequestration. Palms contributed to less than 1 % of the total C stocks. Natives comprised two-thirds of the tree population and sequestered 90 % of all C, while invasive trees and palms accounted for 5 % of net C sequestration. Overall, invasive and exotic trees had a limited contribution to total C stocks and sequestration. Annual tree-related maintenance C emissions were 0.1 % of total gross C sequestration. Plot-level tree, palm, and litter cover were correlated to C stocks and net sequestration. Findings can be used to complement existing urban forest C offset accounting and monitoring protocols and to better understand the role of invasive woody plants on urban ecosystem service provision. [ABSTRACT FROM AUTHOR]