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3 results on '"Tardito D"'

1. cAMP signaling pathway in depressed patients with psychotic features.

Author

Perez, J., Tardito, D., Racagni, G., and Smeraldi, E.

Subjects
*PROTEIN kinases, *MENTAL depression
Abstract

Abnormalities in protein kinase A (PKA) and Rap1 have recently been reported in depressed patients. The aim of the present study was to investigate the levels of these proteins in platelets from untreated unipolar and bipolar depressed patients with psychotic features. The levels PKA and Rap1 were assessed by Western blot analysis and immunostaining in 37 drug-free patients and 29 healthy subjects. Both unipolar and bipolar patients with psychotic depression have significantly lower levels of platelet regulatory type I and higher levels of catalytic subunits of PKA than controls, whereas the levels of regulatory type II were higher only in psychotic unipolar patients. No significant differences were found in the immunolabeling of both Rap1 and actin among groups. These findings support the idea that besides nonpsychotic depression, abnormalities of PKA could be linked, albeit in a somewhat different way, with psychotic depression. [ABSTRACT FROM AUTHOR]

Published
2002
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2. Protein kinase A and Rap1 levels in platelets of untreated patients with major depression.

Author

Perez, J., Tardito, D., Racagni, G., Smeraldi, E., and Zanardi, R.

Subjects
*PROTEIN kinases, *BLOOD platelets, *MENTAL depression
Abstract

Assesses the levels of protein kinase A and Rap1 in platelets from untreated euthymic and depressed patients with major unipolar depression. Altered levels of the genes in patients with bipolar disorder; Use of the Western blot analysis, immunostaining and computer-assisted imaging in the assessment; Immunolabeling in both the regulatory type I and the catalytic subunits of protein kinase A and Rap1.

Published
2001
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3. Early raise of BDNF in hippocampus suggests induction of posttranscriptional mechanisms by antidepressants

Author

Annamaria Cattaneo, Daniela Tardito, Laura Musazzi, Giorgio Racagni, Massimo Gennarelli, Maurizio Popoli, Sergio Barlati, Alessandro Barbon, Musazzi, L, Cattaneo, A, Tardito, D, Barbon, A, Gennarelli, M, Barlati, S, Racagni, G, and Popoli, M

Subjects
Male, medicine.medical_specialty, Transcription, Genetic, Morpholines, Hippocampus, Antidepressant, Pharmacology, Biology, Statistics, Nonparametric, lcsh:RC321-571, Rats, Sprague-Dawley, Reboxetine, Cellular and Molecular Neuroscience, Fluoxetine, Internal medicine, Gene expression, medicine, Animals, Longitudinal Studies, RNA, Messenger, lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry, Brain-derived neurotrophic factor, Analysis of Variance, Messenger RNA, Adrenergic Uptake Inhibitors, hippocampu, Brain-Derived Neurotrophic Factor, General Neuroscience, lcsh:QP351-495, Antidepressive Agents, Rats, lcsh:Neurophysiology and neuropsychology, Endocrinology, Mechanism of action, nervous system, Brain Derived Neurotrophic Factor, gene expression, biology.protein, medicine.symptom, Selective Serotonin Reuptake Inhibitors, Research Article, medicine.drug, Neurotrophin
Abstract

Background The neurotrophin BDNF has been implicated in the regulation of neuroplasticity, gene expression, and synaptic function in the adult brain, as well as in the pathophysiology of neuropsychiatric disorders and the mechanism of action of antidepressants. Antidepressant treatments have been shown to increase the expression of BDNF mRNA, although the changes measured were found to be different depending on various factors. A few studies only have measured levels of BDNF protein after antidepressant treatments, and poor correlation was found between mRNA and protein changes. We studied the time course of expression of BDNF mRNA and protein during drug treatments, in order to elucidate the temporal profile of regulation of this effector and whether mRNA and protein levels correlate. Rat groups were treated for 1, 2 or 3 weeks with fluoxetine or reboxetine; in additional groups drug treatment was followed by a washout week (3+1). Total BDNF mRNA was measured by Real Time PCR, pro- and mature BDNF proteins were measured by Western blot. Results We found that mature BDNF protein is induced more rapidly than mRNA, by both drugs in hippocampus (weeks 1–2) and by reboxetine in prefrontal/frontal cortex (week 1). The temporal profile of BDNF protein expression was largely inconsistent with that of mRNA, which followed the protein induction and reached a peak at week 3. Conclusion These results suggest that BDNF protein is rapidly elevated by antidepressant treatments by posttranscriptional mechanisms, and that induction of BDNF mRNA is a slower process.

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