23 results on '"Saksida, Lisa M."'
Search Results
2. Open science and data sharing in cognitive neuroscience with MouseBytes and MouseBytes+.
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Memar, Sara, Jiang, Eric, Prado, Vania F., Saksida, Lisa M., Bussey, Timothy J., and Prado, Marco A. M.
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COGNITIVE neuroscience ,OPEN scholarship ,INFORMATION sharing ,DATA science ,DATA libraries ,NEUROSCIENCES ,AFFECTIVE neuroscience - Abstract
Open access to rodent cognitive data has lagged behind the rapid generation of large open-access datasets in other areas of neuroscience, such as neuroimaging and genomics. One contributing factor has been the absence of uniform standardization in experiments and data output, an issue that has particularly plagued studies in animal models. Touchscreen-automated cognitive testing of animal models allows standardized outputs that are compatible with open-access sharing. Touchscreen datasets can be combined with different neuro-technologies such as fiber photometry, miniscopes, optogenetics, and MRI to evaluate the relationship between neural activity and behavior. Here we describe a platform that allows deposition of these data into an open-access repository. This platform, called MouseBytes, is a web-based repository that enables researchers to store, share, visualize, and analyze cognitive data. Here we present the architecture, structure, and the essential infrastructure behind MouseBytes. In addition, we describe MouseBytes+, a database that allows data from complementary neuro-technologies such as imaging and photometry to be easily integrated with behavioral data in MouseBytes to support multi-modal behavioral analysis. [ABSTRACT FROM AUTHOR]
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- 2023
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3. Continuous cholinergic-dopaminergic updating in the nucleus accumbens underlies approaches to reward-predicting cues.
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Skirzewski, Miguel, Princz-Lebel, Oren, German-Castelan, Liliana, Crooks, Alycia M., Kim, Gerard Kyungwook, Tarnow, Sophie Henke, Reichelt, Amy, Memar, Sara, Palmer, Daniel, Li, Yulong, Jane Rylett, R., Saksida, Lisa M., Prado, Vania F., Prado, Marco A. M., and Bussey, Timothy J.
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DOPAMINE ,NUCLEUS accumbens ,GLUTAMATE transporters ,REINFORCEMENT learning ,ACETYLCHOLINE ,INTERNEURONS ,LEARNING ability - Abstract
The ability to learn Pavlovian associations from environmental cues predicting positive outcomes is critical for survival, motivating adaptive behaviours. This cued-motivated behaviour depends on the nucleus accumbens (NAc). NAc output activity mediated by spiny projecting neurons (SPNs) is regulated by dopamine, but also by cholinergic interneurons (CINs), which can release acetylcholine and glutamate via the activity of the vesicular acetylcholine transporter (VAChT) or the vesicular glutamate transporter (VGLUT3), respectively. Here we investigated behavioural and neurochemical changes in mice performing a touchscreen Pavlovian approach task by recording dopamine, acetylcholine, and calcium dynamics from D1- and D2-SPNs using fibre photometry in control, VAChT or VGLUT3 mutant mice to understand how these signals cooperate in the service of approach behaviours toward reward-predicting cues. We reveal that NAc acetylcholine-dopaminergic signalling is continuously updated to regulate striatal output underlying the acquisition of Pavlovian approach learning toward reward-predicting cues. Nucleus accumbens cholinergic interneurons release acetylcholine and glutamate. Here, authors show that acetylcholine, rather than glutamate, is predominantly involved in updating dopamine dynamics mediating Pavlovian approach behaviours. [ABSTRACT FROM AUTHOR]
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- 2022
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4. The spontaneous location recognition task for assessing spatial pattern separation and memory across a delay in rats and mice.
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Reichelt, Amy C., Kramar, Cecilia P., Ghosh-Swaby, Olivia R., Sheppard, Paul A. S., Kent, Brianne A., Bekinschtein, Pedro, Saksida, Lisa M., and Bussey, Timothy J.
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- 2021
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5. Age-dependent and region-specific alteration of parvalbumin neurons, perineuronal nets and microglia in the mouse prefrontal cortex and hippocampus following obesogenic diet consumption.
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Reichelt, Amy C., Lemieux, Claire A., Princz-Lebel, Oren, Singh, Ashmita, Bussey, Timothy J., and Saksida, Lisa M.
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PARVALBUMINS ,PREFRONTAL cortex ,HIPPOCAMPUS (Brain) ,HIGH-fat diet ,LABORATORY mice - Abstract
Emergent evidence demonstrates that excessive consumption of high fat and high sugar (HFHS) diets has negative consequences on hippocampal and prefrontal cortex (PFC) function. Moreover, the delayed maturation of the PFC including the late development of parvalbumin-expressing (PV) interneurons and perineuronal nets (PNNs) may promote vulnerability to HFHS diet-induced nutritional stress. However, the young brain may have some resistance to diet-induced neuroinflammation. Thus, we examined the impact of a HFHS diet commencing either in adolescence or adulthood in male mice. PV interneurons, PNNs and microglia were assessed using immunohistochemistry. We observed greater numbers of PV neurons and PNNs in the hippocampus and the prelimbic and infralimbic PFC in adult mice in comparison to our younger cohort. Mice that consumed HFHS diet as adults had reduced numbers of hippocampal PV neurons and PNNs, which correlated with adiposity. However, we saw no effects of diet on PV and PNNs in the PFC. HFHS diet increased microgliosis in the adult cohort, and morphological changes to microglia were observed in the PFC and hippocampus of the adolescent cohort, with a shift to activated microglia phenotypes. Taken together, these findings demonstrate different regional and age-specific effects of obesogenic diets on PV neurons, PNNs and microglia. [ABSTRACT FROM AUTHOR]
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- 2021
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6. Translational tests involving non-reward: methodological considerations.
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Phillips, Benjamin U., Lopez-Cruz, Laura, Saksida, Lisa M., and Bussey, Timothy J.
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REINFORCEMENT learning ,OPERANT behavior ,EXTINCTION (Psychology) ,NEUROTRANSMITTERS ,DOPAMINE ,SEROTONIN ,REWARD (Psychology) ,LABORATORY animals - Abstract
This review is concerned with methods for assessing the processing of unrewarded responses in experimental animals and the mechanisms underlying performance of these tasks. A number of clinical populations, including Parkinson's disease, depression, compulsive disorders, and schizophrenia demonstrate either abnormal processing or learning from non-rewarded responses in laboratory-based reinforcement learning tasks. These effects are hypothesized to result from disturbances in modulatory neurotransmitter systems, including dopamine and serotonin. Parallel work in experimental animals has revealed consistent behavioral patterns associated with non-reward and, consistent with the human literature, modulatory roles for specific neurotransmitters. Classical tests involving an important reward omission component include appetitive extinction, ratio schedules of responding, reversal learning, and delay and probability discounting procedures. In addition, innovative behavioral tests have recently been developed leverage probabilistic feedback to specifically assay accommodation of, and learning from, non-rewarded responses. These procedures will be described and reviewed with discussion of the behavioral and neural determinants of performance. A final section focusses specifically on the benefits of trial-by-trial analysis of responding during such tasks, and the implications of such analyses for the translation of findings to clinical studies. [ABSTRACT FROM AUTHOR]
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- 2019
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7. Impaired object-location learning and recognition memory but enhanced sustained attention in M2 muscarinic receptor-deficient mice.
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Romberg, Carola, Bartko, Susan, Wess, Jürgen, Saksida, Lisa M., and Bussey, Timothy J.
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MUSCARINIC acetylcholine receptors ,MUSCARINIC receptors ,COGNITIVE therapy ,CELL receptors ,ALZHEIMER'S disease - Abstract
Rationale: Muscarinic acetylcholine receptors are known to play key roles in mediating cognitive processes, and impaired muscarinic cholinergic neurotransmission is associated with normal ageing processes and Alzheimer’s disease. However, the specific contributions of the individual muscarinic receptor subtypes (M1-M5) to cognition are presently not well understood.Objectives: The aim of this study was to investigate the contribution of M2-type muscarinic receptor signalling to sustained attention, executive control and learning and memory.Methods: M2 receptor-deficient (M2
−/− ) mice were tested on a touchscreen-operated task battery testing visual discrimination, behavioural flexibility, object-location associative learning, attention and response control. Spontaneous recognition memory for real-world objects was also assessed.Results: We found that M2−/− mice showed an enhancement of attentional performance, but significant deficits on some tests of learning and memory. Executive control and visual discrimination were unaffected by M2-depletion.Conclusions: These findings suggest that M2 activation has heterogeneous effects across cognitive domains, and provide insights into how acetylcholine may support multiple specific cognitive processes through functionally distinct cholinergic receptor subtypes. They also suggest that therapeutics involving M2 receptor-active compounds should be assessed across a broad range of cognitive domains, as they may enhance some cognitive functions, but impair others. [ABSTRACT FROM AUTHOR]- Published
- 2018
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8. Validation and optimisation of a touchscreen progressive ratio test of motivation in male rats.
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Hailwood, Jonathan M., Heath, Christopher J., Robbins, Trevor W., Saksida, Lisa M., and Bussey, Timothy J.
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LABORATORY rats ,RAT behavior ,RODENTS ,SPRAGUE Dawley rats ,ANIMAL welfare - Abstract
Rationale: Across species, effort-related motivation can be assessed by testing behaviour under a progressive ratio (PR) schedule of reinforcement. However, to date, PR tasks for rodents have been available using traditional operant response systems only.Objectives: Touchscreen operant response systems allow the assessment of behaviour in laboratory rodents, using tasks that share high face validity with the computerised assessments used in humans. Here, we sought to optimise a rat touchscreen variant of PR and validate it by assessing the effects of a number of manipulations known to affect PR performance in non-touchscreen paradigms.Methods: Separate groups of male Sprague-Dawley rats were trained on PR schedules with either linear (PR4) or exponential (PREXP) schedules of reinforcement. PR performance was assessed in response to manipulations in reward outcome. Animals were tested under conditions of increased reward magnitude and following reward devaluation through a prefeeding procedure. Subsequently, the effects of systemic administration of the dopamine D2/D3 receptor antagonist raclopride and the psychostimulant d-amphetamine were examined as traditional pharmacological methods for manipulating motivation.Results: Rats reinforced under PR4 and PREXP schedules consistently showed differential patterns of response rates within sessions. Furthermore, both PR4 and PREXP schedules were sensitive to suppression by prefeeding or raclopride administration. Performance under both schedules was facilitated by increasing reward magnitude or d-amphetamine administration.Conclusions: Taken together, these findings mirror those observed in lever-based PR paradigms in rats. This study therefore demonstrates the successful validation of the rat touchscreen PR task. This will allow for the assessment of motivation in rats, within the same touchscreen apparatus used for the assessment of complex cognitive processes in this species. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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9. Selective effects of 5-HT2C receptor modulation on performance of a novel valence-probe visual discrimination task and probabilistic reversal learning in mice.
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Phillips, Benjamin U., Dewan, Sigma, Nilsson, Simon R. O., Robbins, Trevor W., Heath, Christopher J., Saksida, Lisa M., Bussey, Timothy J., and Alsiö, Johan
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SEROTONIN ,NEUROTRANSMITTERS ,MENTAL depression ,AFFECTIVE disorders ,TRYPTAMINE - Abstract
Rationale: Dysregulation of the serotonin (5-HT) system is a pathophysiological component in major depressive disorder (MDD), a condition closely associated with abnormal emotional responsivity to positive and negative feedback. However, the precise mechanism through which 5-HT tone biases feedback responsivity remains unclear. 5-HT2C receptors (5-HT2CRs) are closely linked with aspects of depressive symptomatology, including abnormalities in reinforcement processes and response to stress. Thus, we aimed to determine the impact of 5-HT2CR function on response to feedback in biased reinforcement learning.Methods: We used two touchscreen assays designed to assess the impact of positive and negative feedback on probabilistic reinforcement in mice, including a novel valence-probe visual discrimination (VPVD) and a probabilistic reversal learning procedure (PRL). Systemic administration of a 5-HT2CR agonist and antagonist resulted in selective changes in the balance of feedback sensitivity bias on these tasks.Results: Specifically, on VPVD, SB 242084, the 5-HT2CR antagonist, impaired acquisition of a discrimination dependent on appropriate integration of positive and negative feedback. On PRL, SB 242084 at 1 mg/kg resulted in changes in behaviour consistent with reduced sensitivity to positive feedback. In contrast, WAY 163909, the 5-HT2CR agonist, resulted in changes associated with increased sensitivity to positive feedback and decreased sensitivity to negative feedback.Conclusions: These results suggest that 5-HT2CRs tightly regulate feedback sensitivity bias in mice with consequent effects on learning and cognitive flexibility and specify a framework for the influence of 5-HT2CRs on sensitivity to reinforcement. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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10. The touchscreen operant platform for assessing executive function in rats and mice.
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Mar, Adam C, Horner, Alexa E, Nilsson, Simon R O, Alsiö, Johan, Kent, Brianne A, Kim, Chi Hun, Holmes, Andrew, Saksida, Lisa M, and Bussey, Timothy J
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- 2013
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11. GluN2B in corticostriatal circuits governs choice learning and choice shifting.
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Brigman, Jonathan L, Daut, Rachel A, Wright, Tara, Gunduz-Cinar, Ozge, Graybeal, Carolyn, Davis, Margaret I, Jiang, Zhihong, Saksida, Lisa M, Jinde, Seiichiro, Pease, Matthew, Bussey, Timothy J, Lovinger, David M, Nakazawa, Kazu, and Holmes, Andrew
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IMMUNOCYTOCHEMISTRY ,MICRODIALYSIS ,PREFRONTAL cortex ,NEURONS ,GLUTAMATE receptors - Abstract
A choice that reliably produces a preferred outcome can be automated to liberate cognitive resources for other tasks. Should an outcome become less desirable, behavior must adapt in parallel or it becomes perseverative. Corticostriatal systems are known to mediate choice learning and flexibility, but the molecular mechanisms of these processes are not well understood. We integrated mouse behavioral, immunocytochemical, in vivo electrophysiological, genetic and pharmacological approaches to study choice. We found that the dorsal striatum (DS) was increasingly activated with choice learning, whereas reversal of learned choice engaged prefrontal regions. In vivo, DS neurons showed activity associated with reward anticipation and receipt that emerged with learning and relearning. Corticostriatal or striatal deletion of Grin2b (encoding the NMDA-type glutamate receptor subunit GluN2B) or DS-restricted GluN2B antagonism impaired choice learning, whereas cortical Grin2b deletion or OFC GluN2B antagonism impaired shifting. Our convergent data demonstrate how corticostriatal GluN2B circuits govern the ability to learn and shift choice behavior. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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12. Evolution of GluN2A/B cytoplasmic domains diversified vertebrate synaptic plasticity and behavior.
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Ryan, Tomás J, Kopanitsa, Maksym V, Indersmitten, Tim, Nithianantharajah, Jess, Afinowi, Nurudeen O, Pettit, Charles, Stanford, Lianne E, Sprengel, Rolf, Saksida, Lisa M, Bussey, Timothy J, O'Dell, Thomas J, Grant, Seth G N, and Komiyama, Noboru H
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GENOMES ,VERTEBRATES ,MAMMALS ,BIOCHEMISTRY ,PHYSIOLOGY - Abstract
Two genome duplications early in the vertebrate lineage expanded gene families, including GluN2 subunits of the NMDA receptor. Diversification between the four mammalian GluN2 proteins occurred primarily at their intracellular C-terminal domains (CTDs). To identify shared ancestral functions and diversified subunit-specific functions, we exchanged the exons encoding the GluN2A (also known as Grin2a) and GluN2B (also known as Grin2b) CTDs in two knock-in mice and analyzed the mice's biochemistry, synaptic physiology, and multiple learned and innate behaviors. The eight behaviors were genetically separated into four groups, including one group comprising three types of learning linked to conserved GluN2A/B regions. In contrast, the remaining five behaviors exhibited subunit-specific regulation. GluN2A/B CTD diversification conferred differential binding to cytoplasmic MAGUK proteins and differential forms of long-term potentiation. These data indicate that vertebrate behavior and synaptic signaling acquired increased complexity from the duplication and diversification of ancestral GluN2 genes. [ABSTRACT FROM AUTHOR]
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- 2013
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13. Synaptic scaffold evolution generated components of vertebrate cognitive complexity.
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Nithianantharajah, Jess, Komiyama, Noboru H, McKechanie, Andrew, Johnstone, Mandy, Blackwood, Douglas H, Clair, David St, Emes, Richard D, van de Lagemaat, Louie N, Saksida, Lisa M, Bussey, Timothy J, and Grant, Seth G N
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VERTEBRATES ,ANIMAL cognition ,GENOMES ,GENES ,ANIMAL psychopathology - Abstract
The origins and evolution of higher cognitive functions, including complex forms of learning, attention and executive functions, are unknown. A potential mechanism driving the evolution of vertebrate cognition early in the vertebrate lineage (550 million years ago) was genome duplication and subsequent diversification of postsynaptic genes. Here we report, to our knowledge, the first genetic analysis of a vertebrate gene family in cognitive functions measured using computerized touchscreens. Comparison of mice carrying mutations in each of the four Dlg paralogs showed that simple associative learning required Dlg4, whereas Dlg2 and Dlg3 diversified to have opposing functions in complex cognitive processes. Exploiting the translational utility of touchscreens in humans and mice, testing Dlg2 mutations in both species showed that Dlg2's role in complex learning, cognitive flexibility and attention has been highly conserved over 100 million years. Dlg-family mutations underlie psychiatric disorders, suggesting that genome evolution expanded the complexity of vertebrate cognition at the cost of susceptibility to mental illness. [ABSTRACT FROM AUTHOR]
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- 2013
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14. Assessment of Glutamate Transporter GLAST (EAAT1)-Deficient Mice for Phenotypes Relevant to the Negative and Executive/Cognitive Symptoms of Schizophrenia.
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Karlsson, Rose-Marie, Tanaka, Kohichi, Saksida, Lisa M, Bussey, Timothy J, Heilig, Markus, and Holmes, Andrew
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GLUTAMIC acid ,SCHIZOPHRENIA ,COGNITION ,ANHEDONIA ,NEUROPSYCHOPHARMACOLOGY - Abstract
Glutamatergic dysfunction is increasingly implicated in the pathophysiology of schizophrenia. Current models postulate that dysfunction of glutamate and its receptors underlie many of the symptoms in this disease. However, the mechanisms involved are not well understood. Although elucidating the role for glutamate transporters in the disease has been limited by the absence of pharmacological tools that selectively target the transporter, we recently showed that glial glutamate and aspartate transporter (GLAST; excitatory amino-acid transporter 1) mutant mice exhibit abnormalities on behavioral measures thought to model the positive symptoms of schizophrenia, some of which were rescued by treatment with either haloperidol or the mGlu2/3 agonist, LY379268 the mGlu2/3 agonist, LY379268. To further determine the role of GLAST in schizophrenia-related behaviors we tested GLAST mutant mice on a series of behavioral paradigms associated with the negative (social withdrawal, anhedonia), sensorimotor gating (prepulse inhibition of startle), and executive/cognitive (discrimination learning, extinction) symptoms of schizophrenia. GLAST knockout (KO) mice showed poor nesting behavior and abnormal sociability, whereas KO and heterozygous (HET) both demonstrated lesser preference for a novel social stimulus compared to wild-type littermate controls. GLAST KO, but not HET, had a significantly reduced acoustic startle response, but no significant deficit in prepulse inhibition of startle. GLAST KO and HET showed normal sucrose preference. In an instrumental visual discrimination task, KO showed impaired learning. By contrast, acquisition and extinction of a simple instrumental response was normal. The mGlu2/3 agonist, LY379268, failed to rescue the discrimination impairment in KO mice. These findings demonstrate that gene deletion of GLAST produces select phenotypic abnormalities related to the negative and cognitive symptoms of schizophrenia.Neuropsychopharmacology (2009) 34, 1578–1589; doi:10.1038/npp.2008.215; published online 10 December 2008 [ABSTRACT FROM AUTHOR]
- Published
- 2009
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15. Time-of-day discrimination by pigeons, Columba livia.
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Saksida, Lisa M. and Wilkie, Donald M.
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PIGEON behavior , *TIME perception in animals - Abstract
Examines the time-of-day discrimination by pigeons. Training of pigeons to discriminate four keys; Demonstration of time-place associative learning; Investigation of the mechanisms underlying timing behavior.
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- 1994
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16. The continuous performance test (rCPT) for mice: a novel operant touchscreen test of attentional function
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Kim, Chi Hun, Hvoslef-Eide, Martha, Nilsson, Simon RO, Johnson, Mark R, Herbert, Bronwen R, Robbins, Trevor W, Saksida, Lisa M, Bussey, Timothy J, Mar, Adam C, Robbins, Trevor [0000-0003-0642-5977], and Apollo - University of Cambridge Repository
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Male ,BASAL FOREBRAIN ,Mouse ,Drug Evaluation, Preclinical ,SELECTIVE ATTENTION ,Discrimination Learning ,DBA/2J MICE ,17 Psychology And Cognitive Sciences ,Mice ,Discrimination, Psychological ,Piperidines ,Species Specificity ,MOUSE MODELS ,Animals ,Humans ,Attention ,Donepezil ,Pharmacology & Pharmacy ,Nootropic Agents ,2 INBRED STRAINS ,Psychiatry ,REACTION-TIME-TASK ,Pharmacology ,Science & Technology ,Neurosciences ,11 Medical And Health Sciences ,CHOLINERGIC MECHANISMS ,Touchscreen operant chamber ,SENSITIVITY DECREMENT ,Mice, Inbred C57BL ,ALZHEIMERS-DISEASE ,Mouse strain differences ,Mice, Inbred DBA ,Indans ,Visual Perception ,Conditioning, Operant ,Cholinesterase Inhibitors ,Neurosciences & Neurology ,SUSTAINED ATTENTION ,Continuous performance test ,Life Sciences & Biomedicine ,Psychomotor Performance - Abstract
RATIONALE: Continuous performance tests (CPTs) are widely used to assess attentional processes in a variety of disorders including Alzheimer's disease and schizophrenia. Common human CPTs require discrimination of sequentially presented, visually patterned 'target' and 'non-target' stimuli at a single location. OBJECTIVES: The aims of this study were to evaluate the performance of three popular mouse strains on a novel rodent touchscreen test (rCPT) designed to be analogous to common human CPT variants and to investigate the effects of donepezil, a cholinesterase inhibitor and putative cognitive enhancer. METHODS: C57BL/6J, DBA/2J and CD1 mice (n = 15-16/strain) were trained to baseline performance using four rCPT training stages. Then, probe tests assessed the effects of parameter changes on task performance: stimulus size, duration, contrast, probability, inter-trial interval or inclusion of flanker distractors. rCPT performance was also evaluated following acute administration of donepezil (0-3 mg/kg, i.p.). RESULTS: C57BL/6J and DBA/2J mice showed similar acquisition rates and final baseline performance following rCPT training. On probe tests, rCPT performance of both strains was sensitive to alteration of visual and/or attentional demands (stimulus size, duration, contrast, rate, flanker distraction). Relative to C57BL/6J, DBA/2J mice exhibited (1) decreasing sensitivity (d') across the 45-min session, (2) reduced performance on probes where the appearance of stimuli or adjacent areas were changed (size, contrast, flanking distractors) and (3) larger dose- and stimulus duration-dependent changes in performance following donepezil administration. In contrast, CD1 mice failed to acquire rCPT (stage 3) and pairwise visual discrimination tasks. CONCLUSIONS: rCPT is a potentially useful translational tool for assessing attention in mice and for detecting the effects of nootropic drugs.
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17. The role of the dorsal hippocampus in two versions of the touchscreen automated paired associates learning (PAL) task for mice
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Kim, Chi Hun, Heath, Christopher J., Kent, Brianne A., Bussey, Timothy J., Saksida, Lisa M., and Apollo - University of Cambridge Repository
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Male ,Touch screen operant chamber ,Pharmacology ,Dose-Response Relationship, Drug ,Mouse ,Paired-associate learning ,Muscimol ,Motor Activity ,Hippocampus ,Mice, Inbred C57BL ,Automation ,Mice ,Reward ,Animals ,Conditioning, Operant ,Noise ,GABA Agonists ,Photic Stimulation ,Psychomotor Performance ,Original Investigation - Abstract
Rationale\ud The CANTAB object-location paired-associate learning (PAL) test can detect cognitive deficits in schizophrenia and Alzheimer’s disease. A rodent version of touch screen PAL (dPAL) has been developed, but the underlying neural mechanisms are not fully understood. Although there is evidence that inactivation of the hippocampus following training leads to impairments in rats, this has not been tested in mice. Furthermore, it is not known whether acquisition, as opposed to performance, of the rodent version depends on the hippocampus. This is critical as many mouse models may have hippocampal dysfunction prior to the onset of task training. \ud Objectives\ud The objectives of this study are to examine the effects of dorsal hippocampal (dHp) dysfunction on both performance and acquisition of mouse dPAL and to determine if hippocampal task sensitivity could be increased using a newly developed context-disambiguated PAL (cdPAL) paradigm. \ud Methods\ud In experiment 1, C57Bl/6 mice received post-acquisition dHp infusions of the GABA agonist muscimol. In experiment 2, C57Bl/6 mice received excitotoxic dHp lesions prior to dPAL/cdPAL acquisition. \ud Results\ud Post-acquisition muscimol dose-dependently impaired dPAL and cdPAL performance. Pre-acquisition dHp lesions had only mild effects on both PAL tasks. Behavioural challenges including addition of objects and degradation of the visual stimuli with noise did not reveal any further impairments. \ud Conclusions\ud dPAL and cdPAL performance is hippocampus-dependent in the mouse, but both tasks can be learned in the absence of a functional dHp.
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18. Coexistence of perseveration and apathy in the TDP-43Q331K knock-in mouse model of ALS–FTD.
- Author
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Kim, Eosu, White, Matthew A., Phillips, Benjamin U., Lopez-Cruz, Laura, Kim, Hyunjeong, Heath, Christopher J., Lee, Jong Eun, Saksida, Lisa M., Sreedharan, Jemeen, and Bussey, Timothy J.
- Published
- 2020
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19. Paradoxical reversal learning enhancement by stress or prefrontal cortical damage: rescue with BDNF.
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Graybeal, Carolyn, Feyder, Michael, Schulman, Emily, Saksida, Lisa M, Bussey, Timothy J, Brigman, Jonathan L, and Holmes, Andrew
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COGNITION ,MICE ,PREFRONTAL cortex ,PSYCHOLOGICAL stress ,PSYCHOLOGY - Abstract
Stress affects various forms of cognition. We found that moderate stress enhanced late reversal learning in a mouse touchscreen-based choice task. Ventromedial prefrontal cortex (vmPFC) lesions mimicked the effect of stress, whereas orbitofrontal and dorsolateral striatal lesions impaired reversal. Stress facilitation of reversal was prevented by BDNF infusion into the vmPFC. These findings suggest a mechanism by which stress-induced vmPFC dysfunction disinhibits learning by alternate (for example, striatal) systems. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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20. Continuous performance test impairment in a 22q11.2 microdeletion mouse model: improvement by amphetamine.
- Author
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Nilsson, Simon R. O., Heath, Christopher J., Takillah, Samir, Didienne, Steve, Fejgin, Kim, Nielsen, Vibeke, Nielsen, Jacob, Saksida, Lisa M., Mariani, Jean, Faure, Philippe, Didriksen, Michael, Robbins, Trevor W., Bussey, Timothy J., and Mar, Adam C.
- Published
- 2018
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21. Haploinsufficiency of EHMT1 improves pattern separation and increases hippocampal cell proliferation.
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Benevento, Marco, Oomen, Charlotte A., Horner, Alexa E., Amiri, Houshang, Jacobs, Tessa, Pauwels, Charlotte, Frega, Monica, Kleefstra, Tjitske, Kopanitsa, Maksym V., Grant, Seth G. N., Bussey, Timothy J., Saksida, Lisa M., Van der Zee, Catharina E.E.M., van Bokhoven, Hans, Glennon, Jeffrey C., and Kasri, Nael Nadif
- Abstract
Heterozygous mutations or deletions of the human Euchromatin Histone Methyltransferase 1 (EHMT1) gene are the main causes of Kleefstra syndrome, a neurodevelopmental disorder that is characterized by impaired memory, autistic features and mostly severe intellectual disability. Previously, Ehmt1
+/− heterozygous knockout mice were found to exhibit cranial abnormalities and decreased sociability, phenotypes similar to those observed in Kleefstra syndrome patients. In addition, Ehmt1+/− knockout mice were impaired at fear extinction and novel- and spatial object recognition. In this study, Ehmt1+/− and wild-type mice were tested on several cognitive tests in a touchscreen-equipped operant chamber to further investigate the nature of learning and memory changes. Performance of Ehmt1+/− mice in the Visual Discrimination &Reversal learning, object-location Paired-Associates learning- and Extinction learning tasks was found to be unimpaired. Remarkably, Ehmt1+/− mice showed enhanced performance on the Location Discrimination test of pattern separation. In line with improved Location Discrimination ability, an increase in BrdU-labelled cells in the subgranular zone of the dentate gyrus was observed. In conclusion, reduced levels of EHMT1 protein in Ehmt1+/− mice does not result in general learning deficits in a touchscreen-based battery, but leads to increased adult cell proliferation in the hippocampus and enhanced pattern separation ability. [ABSTRACT FROM AUTHOR]- Published
- 2017
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22. Erratum: Evolution of GluN2A/B cytoplasmic domains diversified vertebrate synaptic plasticity and behavior.
- Author
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Ryan, Tomás J, Kopanitsa, Maksym V, Indersmitten, Tim, Nithianantharajah, Jess, Afinowi, Nurudeen O, Pettit, Charles, Stanford, Lianne E, Sprengel, Rolf, Saksida, Lisa M, Bussey, Timothy J, O'Dell, Thomas J, Grant, Seth G N, and Komiyama, Noboru H
- Subjects
NEUROPLASTICITY ,NEURAL circuitry - Abstract
A correction to the article "Evolution of GluN2A/B cytoplasmic domains diversified vertebrate synaptic plasticity and behavior" in the December 2012 issue is presented which mentions the incorrect citations in the figures.
- Published
- 2013
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23. Measuring cognitive deficits in disabled mice using an automated interactive touchscreen system.
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Morton, A. Jennifer, Skillings, Elizabeth, Bussey, Timothy J., and Saksida, Lisa M.
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LETTERS to the editor ,COGNITION disorders - Abstract
A letter to the editor about an automated interactive touchscreen system for measuring cognitive deficits in disabled mice is presented.
- Published
- 2006
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