4 results on '"Sabariappan, Robin"'
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2. Marker-aided selection and validation of various Pi gene combinations for rice blast resistance in elite rice variety ADT 43.
- Author
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Pandian, Balaji Aravindhan, Joel, John, Nachimuthu, Vishnu Varthini, Swaminathan, Manonmani, Govintharaj, Ponnaiyah, Tannidi, Shalini, and Sabariappan, Robin
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RICE blast disease ,PYRICULARIA oryzae ,RICE varieties ,MICROSATELLITE repeats in plants ,PYRAMIDELLIDAE - Abstract
Rice blast caused by fungal pathogen Pyricularia oryzae has a major impact on reducing yield potential of rice. In this study, homozygous plants were selected using microsatellite markers from the BC3F2
population pyramided with four major genes in elite rice variety ADT 43. Background and selected lines with various blast resistance gene combinations were screened under natural conditions to study the effects of various gene combinations. Upon inspection of lines with different gene combinations, the three-gene pyramided line Pi54+Pi33+Pi1 was found to be highly resistant with the score of 3.3 followed by other three-gene pyramided lines Pi54+Pi2+Pi1 and Pi33+Pi2+Pi1, with the scores of 3.9 and 3.8, respectively. Two-gene pyramided lines Pi54+Pi1, Pi33+Pi1 and Pi2+Pi1 were found to be moderately resistant with a mean score of 4.0 each. In the case of monogenic lines, positive plants for Pi54 performed almost equal to three-gene pyramided lines with a mean score of 3.6. Lines with Pi2 and Pi1 were found to be moderately resistant and moderately susceptible with the mean scores of 4.1 and 4.5, respectively. [ABSTRACT FROM AUTHOR] - Published
- 2018
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3. Analysis of Population Structure and Genetic Diversity in Rice Germplasm Using SSR Markers: An Initiative Towards Association Mapping of Agronomic Traits in Oryza Sativa.
- Author
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Nachimuthu, Vishnu, Muthurajan, Raveendran, Duraialaguraja, Sudhakar, Sivakami, Rajeswari, Pandian, Balaji, Ponniah, Govinthraj, Gunasekaran, Karthika, Swaminathan, Manonmani, K K, Suji, and Sabariappan, Robin
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RICE genetics ,BIOMARKERS ,PLANTS ,PLANT germplasm ,ALLELES in plants ,PLANT gene mapping - Abstract
Background: Genetic diversity is the main source of variability in any crop improvement program. It serves as a reservoir for identifying superior alleles controlling key agronomic and quality traits through allele mining/association mapping. Association mapping based on LD (Linkage dis-equilibrium), non-random associations between causative loci and phenotype in natural population is highly useful in dissecting out genetic basis of complex traits. For any successful association mapping program, understanding the population structure and assessing the kinship relatedness is essential before making correlation between superior alleles and traits. The present study was aimed at evaluating the genetic variation and population structure in a collection of 192 rice germplasm lines including local landraces, improved varieties and exotic lines from diverse origin. Results: A set of 192 diverse rice germplasm lines were genotyped using 61 genome wide SSR markers to assess the molecular genetic diversity and genetic relatedness. Genotyping of 192 rice lines using 61 SSRs produced a total of 205 alleles with the PIC value of 0.756. Population structure analysis using model based and distance based approaches revealed that the germplasm lines were grouped into two distinct subgroups. AMOVA analysis has explained that 14 % of variation was due to difference between with the remaining 86 % variation may be attributed by difference within groups. Conclusions: Based on these above analysis viz., population structure and genetic relatedness, a core collection of 150 rice germplasm lines were assembled as an association mapping panel for establishing marker trait associations. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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4. Development and Genetic Characterization of A Novel Herbicide (Imazethapyr) Tolerant Mutant in Rice (Oryza sativa L.)
- Author
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Neelamraju Sarla, M. S. Sheshshayee, D. Shoba, S. Ramchandar, M. K. Kar, S. Utharasu, M. K. Ramkumar, G. Subhasini, Kuldeep Singh, Sabariappan Robin, R. P. Sharma, S. V. Amitha Mithra, Nirupma Singh, D. Dhivyapriya, Prashant B. Kale, Nitasha Grover, Trilochan Mohapatra, S. Gopala Krishnan, S. Manonmani, R. Valarmathi, M. Raveendran, Pawan Kumar Jayaswal, and Ashok Kumar Singh
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0106 biological sciences ,Imazethapyr ,Population ,Mutant ,Soil Science ,Plant Science ,Upland rice ,lcsh:Plant culture ,01 natural sciences ,Herbicide tolerance ,AHAS ,EMS Mutagenesis ,Botany ,lcsh:SB1-1110 ,education ,Gene ,education.field_of_study ,Acetolactate synthase ,Oryza sativa ,biology ,Bulked segregant analysis ,Wild type ,food and beverages ,04 agricultural and veterinary sciences ,040103 agronomy & agriculture ,biology.protein ,0401 agriculture, forestry, and fisheries ,Original Article ,Rice ,Agronomy and Crop Science ,010606 plant biology & botany - Abstract
Background Increased water and labour scarcity in major rice growing areas warrants a shift towards direct seeded rice cultivation under which management of weeds is a major issue. Use of broad spectrum non-selective herbicides is an efficient means to manage weeds. Availability of rice genotypes with complete tolerance against broad-spectrum non-selective herbicides is a pre-requisite for advocating use of such herbicides. In the present study, we developed an EMS induced rice mutant, ‘HTM-N22‘, exhibiting tolerance to a broad spectrum herbicide, ‘Imazethapyr‘, and identified the mutations imparting tolerance to the herbicide. Results We identified a stable and true breeding rice mutant, HTM-N22 (HTM), tolerant to herbicide, Imazethapyr, from an EMS-mutagenized population of approximately 100,000 M2 plants of an upland rice variety, Nagina 22 (N22). Analysis of inheritance of herbicide tolerance in a cross between Pusa 1656-10-61/HTM showed that this trait is governed by a single dominant gene. To identify the causal gene for Imazethapyr tolerance, bulked segregant analysis (BSA) was followed using microsatellite markers flanking the three putative candidate genes viz., an Acetolactate Synthase (ALS) on chromosome 6 and two Acetohydroxy Acid Synthase (AHAS) genes, one on chromosomes 2 and another on chromosome 4. RM 6844 on chromosome 2 located 0.16 Mbp upstream of AHAS (LOC_Os02g30630) was found to co-segregate with herbicide tolerance. Cloning and sequencing of AHAS (LOC_Os02g30630) from the wild type, N22 and the mutant HTM and their comparison with reference Nipponbare sequence revealed several Single Nucleotide Polymorphisms (SNPs) in the mutant, of which eight resulted in non-synonymous mutations. Three of the eight amino acid substitutions were identical to Nipponbare and hence were not considered as causal changes. Of the five putative candidate SNPs, four were novel (at positions 30, 50, 81 and 152) while the remaining one, S627D was a previously reported mutant, known to result in Imidazolinone tolerance in rice. Of the novel ones, G152E was found to alter the hydrophobicty and abolish an N myristoylation site in the HTM compared to the WT, from reference based modeling and motif prediction studies. Conclusions A novel mutant tolerant to the herbicide “Imazethapyr” was developed and characterized for genetic, sequence and protein level variations. This is a HTM in rice without any IPR (Intellectual Property Rights) infringements and hence can be used in rice breeding as a novel genetic stock by the public funded organizations in the country and elsewhere. Electronic supplementary material The online version of this article (doi:10.1186/s12284-017-0151-8) contains supplementary material, which is available to authorized users.
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