Search

Your search keyword '"Rosalia Lira"' showing total 2 results
Start Over Author "Rosalia Lira" Publisher springer nature
2 results on '"Rosalia Lira"'

1. Cadmium-dependent expression of a new metallothionein identified in Trichomonas vaginalis.

Author

Netzahualcoyotzi, Bryan Alexis, Puente-Rivera, Jonathan, Arreola, Rodrigo, Romero, Julio César Torres, Benitez, Maximo Martínez, Carmona, Rosalia Lira, Reyes, Jorge Antonio Moreno, de Jesús Olivares Trejo, Jose, and Sánchez, María Elizbeth Alvarez

Abstract

Metallothioneins (MTs) have been identified in a wide variety of organisms from bacteria to humans. The biological functions of these MTs have a key role in metalloregulatory metabolism and its expression is induced in response to different stimuli, particularly by divalent metal cations. Also, the action of MTs have been implicated in the survival of pathogens in presence of microbicidal concentration of divalent cations, which allows the establishment of the infection. Trichomonas vaginalis is a protozoan parasite that adapts to the microenvironment of the male urogenital tract, where cations such as zinc (Zn2+) and cadmium (Cd2+) are present. Nevertheless, the molecular mechanisms of metal tolerance and homeostasis is not yet dilucidated in this parasite. In this study, we have identified 4 potential MT-like sequences (tvmt´s) in T. vaginalis genome. Because tvmt-2, -3, and -4 corresponds to truncated partial genes, we characterized the trichomonad tvmt-1 gene. The bioinformatic analyses and the predicted protein (TvMT-1) show similar properties to the reported in other MTs. The expression patterns of tvmt-1 in the presence of several divalent cations (Fe2+, Mn2+, Zn2+ and Cd2+) were analyzed and we demonstrated that Cd2+ induce significantly their expression. By indirect immunofluorescence assays, we corroborated this positive regulation of TvMT-1 in the cytoplasm of parasites grown in the presence of Cd2+. The tvmt-1 promoter contains putative metal responsive elements, which are probably the responsible for the Cd2+-dependent expression of this gene. Our results suggest that tvmt-1 gene encode a metallothionein that may be responsible for the homeostatis and detoxification of Cd+2 in T. vaginalis. [ABSTRACT FROM AUTHOR]

Published
2019
Full Text
View/download PDF

2. Use of dried blood samples for monitoring hepatitis B virus infection

Author

Hilda A. Valdez-Salazar, Ma-Teresa Alvarez-Muñoz, Angelica Maldonado-Rodriguez, Alejandro Gómez-Delgado, Othon Rojas-Montes, Rosalia Lira, Carlos Cano-Dominguez, Martha Eugenia Ruiz-Tachiquín, Onofre Muñoz, and Rocio Torres-Ibarra

Subjects
Adult, Male, Hepatitis B virus, Time Factors, medicine.disease_cause, Polymerase Chain Reaction, Sensitivity and Specificity, Virus, law.invention, lcsh:Infectious and parasitic diseases, Specimen Handling, Young Adult, law, Virology, medicine, Humans, lcsh:RC109-216, Desiccation, Dried blood, Mexico, Polymerase chain reaction, business.industry, Methodology, Temperature, Hepatitis B, Middle Aged, medicine.disease, DNA extraction, Blood, Infectious Diseases, Female, business, Viral hepatitis, Viral load
Abstract

BackgroundHepatitis B virus (HBV) infection is a problem in several regions of the world with limited resources. Blood samples dried on filter paper (DBS) have been successfully used to diagnose and monitor several infectious diseases. In Mexico there is an urgent need for an affordable and easy sampling method for viral load (VL) testing and monitoring of chronic HBV infection. The purpose of this work was to validate the utility of DBS samples for monitoring HBV infection in patients from Mexico City.MethodsMatched samples of plasma and DBS on filter paper from 47 HBV infected patients from the Instituto Mexicano del Seguro Social (IMSS), were included. To evaluate the DNA stability and purity from DBS stored at different temperature conditions, samples from ten patients were stored at 4 degree, 25 degree, and 37 degree C for 7 days. After DBS elution and DNA extraction, the purity of these samples was determined measuring the O.D. rate 260/280. The DBS utility for molecular studies was assessed with PCR assays to amplify a 322 bp fragment from the "a" determinant region of the HBV "S" gene. The VL from all samples was determined to evaluate the correlation between plasma and DBS matched samples.ResultsThe quality of the DNA from DBS specimen is not adversely affected by storage at 4 degree, 25 degree and 37 degree C for up 7 days. Statistical ANOVA analyses did not show any significant difference. The same amplification efficiency was observed between DNA templates from samples stored at different temperatures. The Pearson correlation between the VL from DBS and plasma matched samples was 0.93 (p = 0.01). The SD was 1.48 for DBS vs.1.32 for Plasma, and an average of log10copies/mL of 5.32 vs. 5.53. ANOVA analysis did not show any statistically significant difference between the analyzed groups (p = 0.92).ConclusionThe results provide strong evidence that the isolation and quantification of DNA-HBV from DBS is a viable alternative for patient monitoring, and molecular characterization of the virus variants circulating in Mexico.

Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results