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The colocalization of two calcium-binding proteins, parvalbumin (PV) and calbindin-D28k (CaB), which have been reported to be markers of specific subpopulations of neurons in the central nervous system, with the inhibitory amino acid neurotransmitter γ-amino-butyric acid (GABA) was investigated in neurons of laminae I-IV of the subnucleus caudalis of the rat spinal trigeminal nucleus by using post-embedding immunocytochemical methods. Cells immunoreactive for PV, CaB, and GABA were found in all four laminae of the subnucleus caudalis. A substantial proportion of PV-immunoreactive perikarya were also stained for GABA in laminae II and III (44.8% and 39.8%, respectively). However, the majority of PV-containing neurons in laminae I and IV (100% and 86%, respectively), as well as CaB-immunoreactive cells in all four laminae (98.4%), were GABA-negative. These results show that, in contrast to higher brain centers, PV-, CaB-, and GABA-immunoreactive perikarya represent significantly different populations of neurons in the subnucleus caudalis of the rat. In the light of the present findings, the differences in the neurochemical properties of the subnucleus caudalis of the spinal trigeminal nucleus and the spinal dorsal horn are also discussed. [ABSTRACT FROM AUTHOR]

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Copyright of Fresenius' Zeitschrift für Analytische Chemie is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

For the convenient and sufficiently accurate determination of AsO ions in technical products containing different filling, suspending and colouring materials, as well as impurities-e.g. for the assay of arsenate containing plant protecting materials-the following procedure is recommended. About 5 g of the sample is dissolved, resp. extracted with 4 N hydrochloric acid, and aliquots of the clear filtrate are titrated after elimination of the interfering metal ions by combining them with complexing agents (potassium cyanide and disodium ethylenediamin tetraacetic acid) in a properly buffered medium. The titration with 0.1 M MgSO solution is conducted in the presence of eriochrome black T solution as indicator, first time for eliminating the excess of EDTA added, and afterwards to determine the AsO ions, after adding about 40% of ethanol. The procedure may be useful chiefly for serial routine analyses. [ABSTRACT FROM AUTHOR]

Copyright of Analytical & Bioanalytical Chemistry is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Fresenius' Zeitschrift für Analytische Chemie is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)