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Since its inception in 2002, the EUCAST Antifungal Susceptibility Testing Subcommittee (AFST) has developed and refined susceptibility testing methods for yeast, moulds and dermatophytes, and established epidemiological cut-off values and breakpoints for antifungals. For yeast, three challenges have been addressed. Interpretation of trailing growth in fluconazole susceptibility testing, which has been proven without impact on efficacy if below the 50% endpoint. Variability in rezafungin MIC testing due to laboratory conditions, which has been solved by the addition of Tween 20 to the growth medium in E.Def 7.4. And third, interpretation of MICs for rare yeast with no breakpoints, where recommendations have been established for MIC-based clinical advice. For moulds, refinements include the validation of spectrophotometer reading for A. fumigatus to facilitate objective MIC determination, and for dermatophytes the establishment of a microdilution method with automated reading and a selective medium to minimise the risk of contaminations. Recent initiatives involve development and validation of agar-based screening assays for detection of potential azole and echinocandin resistance in A. fumigatus and Aspergillus species, respectively, and of terbinafine resistance in Trichophyton species. Moreover, the development of a EUCAST guidance document for molecular resistance testing represents an advancement, particularly for identifying target gene alterations associated with resistance. In summary, EUCAST AFST continues to play a pivotal role in standardizing AFST and facilitating accurate interpretation of susceptibility data for clinical decision-making. Adoption of EUCAST breakpoints for commercial test methods, however, requires thorough validation to ensure concordance with EUCAST reference testing species-specific MIC distributions. [ABSTRACT FROM AUTHOR]

Purpose: This study compares the plan quality of high-dose-rate brachytherapy (HDR-BT) and volumetric modulated arc therapy (VMAT) for superficial irradiation of large areas of skin with significant curvature in one or more planes.Methods: A total of 14 patients from two centres previously treated with either HDR-BT or VMAT were retrospectively replanned using the alternative technique. Sites included scalp and lower limbs. Identical computed tomography (CT) scans, clinical target volume (CTV) and organs at risk (OARs) and prescription were used for both techniques. Conformity, skin surface dose and OAR doses were compared.Results: Conformity index was consistently better with VMAT than HDR-BT (p < 0.01). Maximum skin surface dose (D0.1cc) had a higher mean of 49.6 Gy with HDR-BT compared to 31.4 Gy for VMAT (p < 0.01). Significantly smaller volumes of healthy tissue were irradiated with VMAT than with HDR-BT. This can be seen in brain volumes receiving 10, 20 and 30 Gy EQD2 and in extremities receiving 5 and 10 Gy. When close to the volume, the lens received significantly lower doses with VMAT (p < 0.01).Conclusion: In this small sample, VMAT gives equal coverage with lower OAR and skin surface doses than HDR-BT for both scalp and extremities. VMAT is a useful technique for treating large, superficial volumes with significant curvature in one or more planes. [ABSTRACT FROM AUTHOR]

Fungi are recognized as efficient decomposers of biopolymers contained in soil or litter, but not all saprotrophic taxa are equally efficient in accessing various C sources. While many fungi may be considered generalists that are able to utilize complex biomass of plant, bacterial, and fungal origin, it is less clear which of the individual biopolymers that compose these substrates they utilize. Here we analysed fungal communities in forest topsoil enriched in bags with polymers composing plant (cellulose, xylan, glucomannan, pectin, lignin) and fungal (chitin, β-1,3-glucan, and β-1,3-1,6-glucan) biomass along with fungal abundance and the activity of enzymes. There was a high degree of specialization among saprotrophs, each biopolymer being preferred by distinct taxa. White-rot fungi and general saprotrophs were most common on cellulose and xylan, while pectin and lignin-associated communities were dominated by moulds, and animal pathogens were found almost exclusively on chitin. Although several enzymes were produced on all biopolymers, the composition of enzyme pools was significantly different among substrates and different from litter. Activity of endocellulase, β-galactosidase, β-mannosidase, and β-glucosidase significantly correlated with the fungal to bacterial biomass ratio indicating the important role of fungi in their production. The results indicate the high level of specialization among litter-inhabiting fungi and differences in the substrate preference across nutritional guilds of saprotrophic fungi. While most of the litter-inhabiting fungi utilize plant biopolymers, fungal biopolymers are also frequently targeted. [ABSTRACT FROM AUTHOR]

As a matter of fact, the cooling method selection is one of the most important steps in the design of injection mould. However, inappropriate cooling system will result in many undesired defects such as differential shrinkage and warpage on the moulded part. From this point of view, the comprehensive study of cylindrical void method (CVM) has been attempted in this study which is known as an alternative effective cooling method. Therefore, this study employs the three-dimensional time-dependent numerical analysis to determine the performance of cooling injection moulding. Initially, a finite element method is used to solve the system of equations of the flow and heat transfer problem. Subsequently, the temperature fields and other analysis results have been obtained via ANSYS Workbench. The study reveals that the Nusselt number, Biot Number and heat flux at the fluid–core interface are smaller when the CVM method is being used compared to the straight-drilled method. These results are mainly attributed to the presence of big vortices which prevent a complete heat transfer. Consequently, the use of the CVM method does not improve the cooling efficiency, but it is a good idea and requires further investigation. [ABSTRACT FROM AUTHOR]

This study reports the microbial quality of ethnic starter culture Xaj-pitha used for rice wine fermentation in Assam. Here, we collected 60 Xaj-pitha samples belonging to Ahom community of the state and enumerated the microorganisms using spread plate technique. Illumina-based whole genome shotgun sequencing detected the presence of microbial contaminants like Acidovorax, Herbaspirillum, Methylobacterium, Pantoea, Pseudomonas, Stenotrophomonas, Staphylococcus, Micrococcus, Acinetobacter, etc. Presence of major health hazards associated with spontaneous rice wine fermentation necessitated method optimization through the development of a defined mixed starter culture. For this, functionally important α-amylase producers viz., Penicillium sp. ABTSJ23, Rhizopus oryzae ABTSJ63, Mucor guilliermondii ABTSJ72 and Amylomyces rouxii ABTSJ82 and eight yeasts viz., Saccharomyces cerevisiae ABTY1J, ABTY1S, ADJ5 & ADJ1, Wickerhamomyces anomalus ADJ2, Saccharomycopsis malanga ADJ3, Saccharomycopsis fibuligera ADJ4 and Saccharomycopsis malanga ADJ6 were retrieved using appropriate media. All the mould cultures tested negative for aflotoxins production. Among the yeasts, Saccharomyces cerevisiae ABTY1S and ADJ1 decarboxylated lysine HCl and tyramine HCl, respectively, indicating their biogenic amine production ability. For defined mixed starter culture, Amylomyces rouxii ABT82 with α-amylase (5.92 U/ml) and glucoamylase (7.50 U/ml) activities was selected as fungal partner; while Saccharomycopsis fibuligera ADJ4 and Saccharomyces cerevisiae ABT-Y1J with high ethanol production (up to 10.11% and 9.88% v/v, respectively) were selected as yeast partners. The mixed culture was able to produce high amount of glucose, ethanol and liquid (glucose 10.91% w/v; ethanol 7.5% w/v; liquid 51.0% w/v). Therefore, this study demonstrated the efficiency of mixed starter cultures for safe and controlled rice wine production. [ABSTRACT FROM AUTHOR]

A 10-week study was conducted to assess the impact of mould infestation on nutrient stability of feed and the mitigating effect of supplemental tocopherol, retinol or a multivitamin on performance and hepatic histology of ISA Brown laying chickens. Two batches of corn were obtained: the aflasafe corn used in preparing control diet and corn with physical evidence of mould used in preparing diets 2 to 5 containing no supplemental vitamin, tocopherol, retinol or a branded multivitamin supplementations respectively. One hundred and fifty (150) laying chickens used were completely randomized into five dietary treatments with three replicates of 10 birds each. Results showed that there was gross instability in the nutrients of mouldy maize. The activities of the fungi depleted both protein and lipid contents by 11.54% and 12.72% respectively while crude fibre content rose by 31.7%. There was substantial drop in both retinol and tocopherol while aflatoxin content rose to 267 μg in mouldy corn and 118 μg in the mouldy diets. Feed intake was significantly (P < 0.05) reduced and consequently depressed (P < 0.05) egg production and feed efficiency. Egg quality differs (P < 0.05) in shell thickness and yolk colour. Proliferation of biliary duct epithelium, hepatic degeneration, cellular infiltration, hyper-cellularity or dilation of the sinusoidal spaces characterized livers of birds on mouldy corn diets while supplementation with vitamins subverted mycosis and aflatoxicosis as evidenced by normal-to-mild congestion of hepatocytes. It was concluded that mould contamination in feed compromised feed nutritive values, reduced bird performance and adversely impaired the liver of the experimental birds while tocopherol, retinol or a multivitamin supplementation relapses the damaging potential of mould and mycotoxin differently. [ABSTRACT FROM AUTHOR]

Sphagnum-dominated ecosystem plays major roles as carbon sinks at the global level. Associated microbial communities, in particular, eukaryotes, play significant roles in nutrient fixation and turnover. In order to understand better the ecological processes driven by these organisms, the first step is to characterise these associated organisms. We characterised the taxonomic diversity, and from this, inferred the functional diversity of microeukaryotes in Sphagnum mosses in tropical, subtropical and temperate climatic zones through an environmental DNA diversity metabarcoding survey of the V9 region of the gene coding for the RNA of the small subunit of the ribosomes (SSU rRNA). As microbial processes are strongly driven by temperatures, we hypothesised that saprotrophy would be highest in warm regions, whereas mixotrophy, an optimal strategy in oligotrophic environments, would peak under colder climates. Phylotype richness was higher in tropical and subtropical climatic zones than in the temperate region, mostly due to a higher diversity of animal parasites (i.e. Apicomplexa). Decomposers, and especially opportunistic yeasts and moulds, were more abundant under warmer climates, while mixotrophic organisms were more abundant under temperate climates. The dominance of decomposers, suggesting a higher heterotrophic activity under warmer climates, is coherent with the generally observed faster nutrient cycling at lower latitudes; this phenomenon is likely enhanced by higher inputs of nutrients most probably brought in the system by Metazoa, such as arthropods. [ABSTRACT FROM AUTHOR]

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Bursitis is a common medical condition that can occur either with or without infection. We present a case of fungal olecranon bursitis in an immunocompetent individual caused by the new species Knoxdaviesia dimorphospora. It is a dematiaceous filamentous fungus characterized by the production of two different conidia: hyaline and cylindrical, which rise up from phialidic conidiogenous cells located in the upper part of differentiated and unbranched conidiophores, and pale brown and ellipsoidal conidia produced by phialidic conidiogenous cells which are born directly on hyphae. In addition to its morphological peculiarities, the novelty of the fungus was confirmed by sequence analysis of the internal transcribed spacer (ITS) regions and D1/D2 domains of the 28S of the nuclear rRNA gene. The fungal infection was confirmed by cytological examination and repeated cultures. The infection was resolved by surgical debridement and drainage, and the patient presented a complete functional recovery 3 months later. The in vitro antifungal susceptibility to this new human opportunist is provided, terbinafine being the drug with the most potent activity. [ABSTRACT FROM AUTHOR]

Purpose: To investigate the relationships between symptoms compatible with the sick building syndrome (SBS) in adults and building dampness and ventilation in single-family houses. Methods: Within the Swedish BETSI study, a national sample of single-family houses were inspected by professional building experts, and adults living in the houses answered a questionnaire on SBS. Relationships between building factors and SBS were analysed using logistic regression. Results: Of the respondents, 23% reported having had weekly SBS symptoms during the last three months. A large proportion of houses exhibited building or construction problems. In total, 40% of houses had dampness problems in the foundation, and this was related to a higher prevalence of both mucous and dermal symptoms, and any SBS symptoms. Furthermore, high air humidity was related to more symptoms, with the relationship with absolute humidity being stronger than that with relative humidity or moisture load. Symptoms were also more prevalent in houses with a high U value, reflecting a poor thermal insulation. Compared to natural ventilation, living in a house with mechanical supply and exhaust ventilation was related to a lower prevalence of general symptoms and any SBS symptoms, but there were only weak associations between measured air exchange rate and symptoms. Conclusions: A large proportion of single-family houses have dampness problems in the foundation, and pollutants may enter the living space of the house and affect the health of the occupants. Furthermore, absolute air humidity should be measured more often in indoor air studies. [ABSTRACT FROM AUTHOR]

Stored wheat grain from across Australia was tested for the presence of postharvest fungi by measuring the production of volatile organic compounds (VOCs). Headspace analysis combined with gas chromatography flame ionization detection (GC/FID) and gas chromatography-mass spectrometry (GC-MS) was used to detect fungi in the grain, based on identification of peaks associated with species such as Alternaria alternata and A. infectoria. A total of 57 possible compounds were detected by GC/FID. Grain from Queensland, Victoria and New South Wales produced less VOCs compared to grain from South Australia and Western Australia. These compounds appeared at both the early and later stages of fungal growth. For example, A. alternata produced volatiles after two days that were only produced by A. infectoria after seven days. Gamma-irradiated grain (controls) did not produce these VOCs. A further analysis of the 57 VOCs by GC-MS identified three VOCs: Cyclooctasiloxane, hexadecamethyl-(CAS 556-68-3) produced by A. alternata, and Pentadecane (CAS 629-62-9) produced by A. infectora. We conclude that fungal VOCs may be useful for early detection of postharvest fungi in grain, prior to visual symptoms occurring. [ABSTRACT FROM AUTHOR]

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3D printing is such a magical technology that it extends into almost every sector relating to manufacturing, not to mention casting production. In this paper, the past, present and future of 3D printing in the foundry sector are profoundly reviewed. 3D printing has the potential to supplement or partially replace the casting method. Today, some castings can be directly printed by metal powders, for example, titanium alloys, nickel alloys and steel parts. Meanwhile, 3D printing has found an unique position in other casting aspects as well, such as printing the wax pattern, ceramic shell, sand core, sand mould, etc. Most importantly, 3D printing is not just a manufacturing method, it will also revolutionize the design of products, assemblies and parts, such as castings, patterns, cores, moulds and shells in casting production. The solid structure of castings and moulds will be redesigned in future into truss or spatially open and skeleton structures. This kind of revolution is just sprouting, but it will bring unimaginable impact on manufacturing including casting production. Nobody doubts the potential of 3D printing technologies in manufacturing, but they do have limitations and drawbacks. [ABSTRACT FROM AUTHOR]

In the last few decades, extreme weather events mostly characterized by more intense and frequent precipitation and wind incidents have risen, and current climatic projections strongly suggest that this trend will keep its pace in the future. These climatic conditions pose additional hygrothermal loading onto the built environment, which may lead to moisture induced deterioration and even strength reduction in masonry. Despite their already long service lives, this impact may in time become problematic for historic buildings, which, by nature of their design and construction, interact with moisture and climate to a far greater degree than modern buildings. The PARNASSUS project was set up with the aim of identifying and quantifying the impact of environment on historic buildings in the face of a changing climate. As part of this project, an extensive environmental monitoring campaign was carried out between 2012 and 2014 at Blicking Hall, a National Trust property in Norfolk, England, with the aim of providing further insight into the building's overall performance under hygrothermal loading. Environmental monitoring work targeted not only indoor and outdoor conditions, but provided information about the temperature and relative humidity profiles across the walls by means of in-wall probes. Wind and rain gauges helped quantify other environmental parameters. This paper presents the findings from this environmental monitoring work, with specific emphasis on the basement suffering from sustained saturation in vapour phase and resulting biological growth, as well as the Long Gallery, where Blickling's valuable library is kept. [ABSTRACT FROM AUTHOR]

A survey was undertaken to determine the mycoflora associated with stored wheat in four states across Australia including Western Australia, South Australia, Victoria and New South Wales. A total of 482 fungal isolates from 15 genera were isolated. The most common genera isolated were Alternaria spp., Aspergillus sp., Aureobasidium sp., Cladosporium spp., Drechslera sp., Fusarium spp., Mucor sp., Nigrospora sp., Penicillium sp., Rhizopus sp., Stemphylium sp., Eutiarosporella spp., Ulocladium sp., Epicoccum sp., and an undescribed genus from the Hypocreales. Diversity profiling also identified fungi that were not isolated using traditional methods including Paecilomyces spp., Dendryphiella and Cryptococcus. The results indicate that the mycoflora of stored wheat varies between Australian grain growing regions, and that diversity profiling analysis identifies different fungal contaminants compared to traditional methods. This study also revealed that some pathogens of high risk to humans (e.g. Cryptococcus) may occur with a high frequency in wheat. [ABSTRACT FROM AUTHOR]

Background: Surveys were conducted to determine awareness of mould infection in maize and groundnut in Kilosa District, Eastern Central Tanzania, between August and October 2010. Four villages of Msingisi, Rudewa-Batini, Mamoyo and Mkalama were surveyed. We sampled maize from each household for mycotoxins contamination after nine months of storage. A questionnaire was administered to heads of 72 selected households in the study area. Principal component analysis (PCA) was used to check factor dimensionality. The differences in mean total score of knowledge and awareness between the demographic and socio-economic factors were determined by t-test. Measurements of association were carried by Chi-square test ( χ2), while ordinal logistic regression analysis was used to examine the correlations between social variables and awareness of mould infection. Results: We found high levels of fumonisins in maize that ranged from 70.46 to 213.15. Aflatoxin levels in groundnuts ranged from 72.97 to 195.17. Result showed that 97 % of respondents were not aware of mould infection in stored maize and groundnuts. The mean score of mould infections awareness of females (1.7273 ± 0.45055) was significantly higher than that of males (1.5714 ± 13.7). Awareness of mould infection was high among educated (mean score 1.9231.3 ± 0.31470) than the less educated people (mean score 1.8913 ± 0.27175). Results revealed significant association between awareness of mould infection with age ( p = 0.006), education ( p = 0.039), marital status ( p = 0.012), gender (0.037) and income ( p = 0.044). Conclusion: Campaigns are needed to improve awareness of mould infection in Kilosa District, Tanzania. [ABSTRACT FROM AUTHOR]

Purpose: To study associations between fungal DNA in day care centres, fractional exhaled nitric oxide (FeNO) and inflammatory markers in day care centre staff. Methods: Totally, 62 staff (90 %) from five day care centres in Sweden participated. All were females. Settled dust was collected and analysed for five sequences of fungal DNA by quantitative PCR. Levels of FeNO (NIOX MINO 50 ml/min) and serum levels of eosinophilic cationic protein, myeloperoxidase (MPO) and high-sensitivity C-reactive protein in blood (HsCRP) were measured. Dynamic spirometry was performed, and dyspnoea was measured. Biomarkers and dyspnoea ratings were log-transformed, and associations were analysed by linear mixed models, adjusting for age, atopy, smoking, body mass index (BMI), ETS and dampness/mould at home. Results: Geometric mean (GM) for FeNO was 15.3 ppb, 6 % were smokers, 14 % were obese, 31 % were overweight and 18 % had atopy. GM concentration was 2.16 × 10 cell equivalents (CE)/g for total fungal DNA, 2310 CE/g for Aspergillus/penicillium (Asp/Pen) DNA, 17 CE/g for Aspergillus versicolor DNA and 14 CE/g dust for Streptomyces DNA. FeNO was associated with total fungal DNA ( p = 0.004), Asp/Pen DNA ( p = 0.005) and Streptomyces DNA ( p = 0.03). HsCRP was associated with total fungal DNA ( p = 0.03) and BMI ( p = 0.001). Dyspnoea was associated with Asp/Pen DNA ( p = 0.04). Subjects with ETS at home had lower lung function (FEV) ( p = 0.03), and those with dampness/mould at home had lower MPO ( p = 0.03). Conclusion: Fungal contamination in day care centres, measured as fungal DNA, can be a risk factor for airway inflammation, and CRP is associated with BMI. [ABSTRACT FROM AUTHOR]

This paper analytically develops a type of model for predicting material removal depth in large mould polishing with coated abrasives. This model based on the statistical theory, and the abrasive material surface contact mechanics is established. The material removal depth is calculated by integrating the linear removal intensity or the removal depth per unit time along the polishing path. The material removal depth profiles of the circular abrasive tool and the annular abrasive tool are presented when polishing paths are a straight line and a curve, respectively. The effects of process parameters on the material removal depth are simulated and analyzed, such as polishing pressure, feed rate, tool speed, and internal radius, when the polishing path is a straight line. The workpiece surfaces after milling were polished by using annular abrasive tool moving along a straight line in the experiment. This model is evaluated by comparing the theoretical material removal depth with those experimental results available. It is concluded that the experiment results are approximately consistent with the model predictions. [ABSTRACT FROM AUTHOR]

There has been a remarkable increase recently in the isolation of fungi such as Aspergillus fumigatus, species of the Pseudallescheria boydii/ Scedosporium apiospermum complex, and Exophiala sp. from the respiratory tract of patients with cystic fibrosis (CF). This review describes the recent insights into the epidemiology, ecology, and physiopathology of the filamentous fungi able to colonize and/or to infect the airways of CF patients, and that may be responsible for accelerated lung function decline. We summarize salient features not only on highly prevalent species such as Aspergillus and Scedosporium, but also on more recently described fungi such as Rasamsonia argillacea. In addition, we discuss the challenges inherent in tracking and interpreting rates of fungal colonization/infection in the CF patient population, taking into consideration the polymicrobial composition of the CF lung environment, the corresponding cross-kingdom interactions, and the new concept of mycobiota. Further research is warranted to clarify the role of fungi in CF lung disease and its therapeutic management. [ABSTRACT FROM AUTHOR]

Asp f 1 (ribotoxin) is the main allergen of Aspergillus fumigatus and a critical factor in provoking allergic responses and bronchopulmonary aspergillosis. This study investigated the prevalence of allergen Asp f 1 in dust samples collected at two Croatian sawmills from different working sites (sawmilling, parquetry and sorting). A total of thirty-five floor dust samples were collected, extracted, and the mass fraction of Asp f 1 was determined using a commercially available enzyme-linked immunosorbent assay. More than 91 % of the collected dust samples had detectable levels of Asp f 1 (limit detection 3.6 ng g). The median Asp f 1 mass fractions in Sawmill 1 and Sawmill 2 were 49.4 ng g (range <3.6-120 ng g) and 35.5 ng g (range 15.1-77.2 ng g), respectively. At both sawmills, higher median Asp f 1 values were found in the dust from sawmilling than from parquetry sites. These preliminary findings suggest the abundance of allergen Asp f 1 in reservoir dust in the woodworking environments of both sawmills. Assessment of Asp f 1 in future studies will provide further insight into the role of this allergen in eliciting respiratory morbidity. [ABSTRACT FROM AUTHOR]

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Objectives: This study aimed to determine that home environmental factors were associated with atopic dermatitis in Japanese elementary school children. Methods: In this cross-sectional study, a total of 4,254 children in 12 public elementary schools in Sapporo city in Hokkaido, Japan were examined. Atopic dermatitis was defined using the International Study of Asthma and Allergies in Childhood questionnaire. The questionnaires also contained 14 questions about the child's home environment. To obtain multivariate-adjusted ORs for atopic dermatitis in relation to the home environment, we controlled for possible confounders including gender, school grade, parental history of allergies, number of siblings, and whether the child was firstborn. The study participants were then divided into two groups according to gender, and a stratified analysis was performed to obtain adjusted ORs for atopic dermatitis in relation to the home environment. Results: The prevalence of atopic dermatitis in our sample was 16.7 %. Using fully adjusted models, the risk factors for atopic dermatitis were found to be the household use of a non-electric heating system without a ventilation duct to the outside (compared to the use of an electric heating system), having visible mould in the house, having a mouldy odour in the house, and condensation on the windowpanes in the house odds ratios (OR 1.25-1.54). In our stratified analysis, having visible mould and having a mouldy odour in the house were relevantly found to be risk factors for boys (OR 1.28-1.64). However, these associations were not found among girls. Conclusions: To improve children's health, further study is needed to corroborate the findings. [ABSTRACT FROM AUTHOR]

Background: Despite recent studies failing to demonstrate the value of routine chest radiography (CXR) in the initial evaluation of the febrile neutropenic patient with cancer, this screening test is advocated by some experts. We evaluated the benefits of CXR for early diagnosis of pulmonary infection at St. Jude Children's Research Hospital (SJCRH) with emphasis on early recognition of mould infections. Patients and methods: We reviewed the courses of 200 consecutive febrile neutropenic pediatric patients to determine if routine CXR at initial evaluation was useful in the identification of clinically occult pneumonia. We also reviewed all cases of proven or probable mould infections from the opening of SJCRH in 1962 until 1998 when routine CXR was no longer practiced in our institution to identify cases that were first recognized by routine CXR. Results: Of 200 febrile neutropenic patients, pulmonary abnormalities consistent with pneumonia were detected by routine CXR in only five patients without pulmonary signs or symptoms. In only one case was a change in management considered. Of the 70 patients with pulmonary mould infection identified from 1962 to 1998, routine CXR was performed in 45 patients at the onset of a febrile, neutropenic episode in which a mould infection was diagnosed. Routine CXR was pivotal in the recognition of the mould infection in only two cases over this 36-year period. Conclusion: CXR is warranted in the evaluation of the newly febrile neutropenic pediatric oncology patient only when respiratory signs or symptoms are present. [ABSTRACT FROM AUTHOR]

In this study, three different rat hybridoma cell lines secreting monoclonal antibodies (mAbs) recognizing the spores from Alternaria alternata, a plant pathogenic fungus, contaminant of food products and important cause of both allergic rhinitis and asthma, have been characterized. These three mAbs are all of IgM isotype. Two antibodies, A1 and F10, were cross-reactive antibodies recognizing spores from Alternaria, Cladosporium, Penicillium, Aspergillus and Stachybotrys genera, but not the yeasts Saccharomyces cerevisiae or Candida albicans. Competitive and sandwich assays demonstrated that these two mAbs were directed against the same or very close repetitive(s) epitope(s). A1-based sandwich ELISA efficiently detected this epitope in various mould (but not yeast)-soluble extracts prepared from strains grown in the laboratory. Moreover, this A1-based sandwich ELISA detected its cognate epitope in air and dust samples obtained from dwellings. The third antibody, E5, recognized only the spores of Alternaria and the phylogenetically very close Ulocladium botrytis. This E5 antibody is directed against a repetitive epitope found in Alternaria and Ulocladium laboratory extracts and can be used in a sandwich assay for the quantification of these moulds. Therefore, E5 antibody is a promising tool for the development of Alternaria- Ulocladium-specific immunoassays, while A1 and F10 could be interesting tools for the quantification of the total mould biomass. [ABSTRACT FROM AUTHOR]

Colemanite, also called as hydrated calcium borate hydroxide, is a borate mineral found in evaporite deposits of alkaline lacustrins environments. It is a secondary mineral that forms by alternation of borax and ulexite. As this chemical is not toxic to human beings and other mammals, it has been chosen for the present preliminary work on assessing the efficacy against mould fungi on rubber wood veneers. Colemanite at two different levels of concentrations, 3 and 5 % was tested against moulds and it has been found that 5 % concentration acts as an effective mouldicide. In India this is the first report where colemanite was used against mould fungi. [ABSTRACT FROM AUTHOR]

This study was undertaken to investigate how the length of the extraction period influences the (1 → 3)-β- d-glucan (β-glucan) yield and also to examine the background concentration of β-glucan as airborne β-glucan in outdoor environments in different seasons and as concentrations in airborne and floor dust in offices. To ensure compatibility between results obtained in different laboratories, it is important to use optimal and standardised methods to extract and quantify β-glucan. In this study, an extraction period of 60 min gave the highest β-glucan yield. The median concentration of β-glucan in 44 floor dust samples was 597 μg g dust. The median concentration of airborne β-glucan in offices was 5.1 ng m in the summer and 2.3 ng m in the winter, and the outdoor median concentration in towns was 6.8 ng m. The outdoor airborne concentration of β-glucan was significantly lower in January, November and December than during the rest of year. In July, the median airborne concentration of β-glucan was 14 times higher than in January. Furthermore, the airborne concentration of β-glucan was significantly higher in July than in March, April, May, September and October. In the summertime, we found that the indoor airborne concentration of β-glucan was lower than outdoor concentrations. This is in accordance with measurements of concentrations of airborne pollen and culturable fungal spores showing higher outdoor than indoor concentrations during the summer months. [ABSTRACT FROM AUTHOR]

There usually exist narrow-long-deep areas in mould needed to be machined in special machining. To identify the narrow-deep areas automatically, an automatic narrow-deep feature (NF) recognition method is put forward accordingly. First, the narrow-deep feature is defined innovatively in this field and then feature hint is extracted from the mould by the characteristics of narrow-deep feature. Second, the elementary constituent faces (ECF) of a feature are found on the basis of the feature hint. By means of extending and clipping the ECF, the feature faces are obtained incrementally by geometric reasoning. As a result, basic narrow-deep features (BNF) related are combined heuristically. The proposed NF recognition method provides an intelligent connection between CAD and CAPP for machining narrow-deep areas in mould. [ABSTRACT FROM AUTHOR]

Modern CAD/CAM techniques together with five-axis high-speed milling allow to reduce moulds manufacturing time and costs. Nevertheless, in order to put a mould into use, operations of manual finishing and fitting are still always required. Such operations, performed manually by mould makers, modify the milled surfaces of moulds. Reverse engineering techniques can be employed in quality control to evaluate dimensions and geometrical tolerances on moulds after machining and fitting. Changes in the product’s shape are sometimes decided after a mould has already been machined. In such cases, if possible, the mould maker directly modifies the mould. Thus the final real geometry of the mould does not reflect the one of the original CAD model. The aim of this paper is to point out the benefits of non-contact quality control and to illustrate a procedure, based on reverse engineering techniques, to reconstruct and update the mathematical model of the mould after it has been polished and fit. The procedure was tested on a mould for the production of a plastic camera body that was previously inspected by means of a structured light scanner. [ABSTRACT FROM AUTHOR]

Few epidemiological studies report on reliability of exposure measurements even though this significantly influences the results of correlation and regression analysis often used in these studies. Poor reliability of exposure measurement reduces the ability to detect a true association between a certain component and health outcome variables. The aim of this study was to determine the ratio of the within-home and between-home components of variance of a number of biocontaminants measured in house dust in the framework of an international study conducted in the Netherlands, Germany and Sweden (the AIRALLERG study). To this end, duplicate dust samples were collected from children's beds and from living room floors in over 100 homes. Samples were taken at the same point in time. Variables considered were the dust mass collected in mg/m2 and the concentrations of the house dust mite allergens Der p 1 and Der f 1, cat allergen Fel d 1, endotoxin, (1 → 3)-β-D-glucan and extracellular polysaccharides, all per gram of dust and per square meter of sampling surface. An analysis of variance showed that the within-home variance was small compared to the between-home variance for most variables (mostly less than half) with the exception of glucan on mattresses, when expressed in μg/g. Investigation of variation over time is needed for a more complete assessment of the use of these variables in epidemiological analyses of exposure–response relationships.Journal of Exposure Science and Environmental Epidemiology (2007) 17, 134–140. doi:10.1038/sj.jes.7500482; published online 5 April 2006 [ABSTRACT FROM AUTHOR]

In this paper, the three-dimensional transient model of the mould temperature is established and the temperature variation is analyzed when the thickness of the mould wall and the cooling rates are different. For the numerical computation, a corresponding finite element method (FEM) program is developed with FORTRAN language, and six-face and eight-node isoparameter elements are applied; the Newton method is used to solve the heat equations. The experimental data have been used to validate the model. It is shown that the appropriate mould wall thickness is 10 mm and the different parts of the mould should correspond with cooling water of different rates. [ABSTRACT FROM AUTHOR]

Objectives: To investigate the occupational exposure and sensitization to storage mites (SM) in sales staff working in a moisture-damaged and three healthy reference buildings. Methods: The study population consisted of the entire personnel ( n=12) in the moisture-damaged grocery store. They all suffered from persistent upper respiratory tract symptoms. Twelve (in results 11) symptom-free controls working in three healthy reference groceries were matched with age, sex and occupation. Dust samples from each building were examined for mites. The clinical study consisted of otorhinolaryngological examination and determination of IgE reactivity. Specific serum IgE antibodies were measured against three SMs and two house dust mites (HDM). Skin prick tests (SPT) were made to the same five mites and to five common aeroallergens. If sensitization to any of the SMs was detected, a nasal provocation test (NPT) was performed. Results: SMs were found in all buildings. In all, seven cases and four control subjects showed IgE-mediated reactivity. Sensitization to mites was detected in six cases and in three controls and in 2/12 and 3/11 this was the only IgE antibody response observed. In addition, one case and one control subject were sensitized to common aeroallergens. NPT with SMs was positive in four cases and in one control. Conclusions: In grocery stores, the personnel are exposed to SMs. The risk of sensitization to mites is obvious and an IgE response can occur without any reactivity to common aeroallergens. SM allergy may in some cases explain the chronic rhinitis related to moisture-damaged buildings. [ABSTRACT FROM AUTHOR]

Voriconazole (Vfend™) is a new triazole that currently is undergoing phase III clinical trials. This review summarizes the published data obtained by NCCLS methods on the in vitro antifungal activity of voriconazole in comparison to itraconazole, amphotericin B, fluconazole, ketoconazole and flucytosine. Voriconazole had fungistatic activity against most yeasts and yeastlike species (minimum inhibitory concentrations [MICs] <2 μg/ml) that was similar or superior to those of fluconazole, amphotericin B, and itraconazole. Against Candida glabrata and C. krusei, voriconazole MIC ranges were 0.03 to 8 and 0.01 to >4 μg/ml, respectively. For four of the six Aspergillus spp. evaluated, voriconazole MICs (< 0.03 to 2 μg/ml) were lower than amphotericin B (0.25 to 4 μg/ml) and similar to itraconazole MICs. Voriconazole fungistatic activity against Fusarium spp. has been variable. Against F. oxysporum and solani, most studies showed MICs ranging from 0.25 to 8 μg/ml. Voriconazole had excellent fungistatic activity against five of the six species of dimorphic fungi evaluated (MIC90s < 1.0 μg/ml). The exception was Sporothrix schenckii (MIC90s and geometric mean MICs ≥ 8 μg/ml). Only amphotericin B had good fungistatic activity against the Zygomycetes species (voriconazole MICs ranged from 2 to >32 μg/ml). Voriconazole showed excellent in vitro activity (MICs < 0.03 to 1.0 μg/ml) against most of the 50 species of dematiaceous fungi tested, but the activity of all the agents was poor against most isolates of Scedosporium prolificans and Phaeoacremonium parasiticum (Phialophora parasitica). Voriconazole had fungicidal activity against most Aspergillus spp., B. dermatitidis, and some dematiaceous fungi. In vitro/in vivo correlations should aid in the interpretation of these results. [ABSTRACT FROM AUTHOR]

Objective: Hypersensitivity pneumonitis due to dry sausage mould has been reported in workers who brush off the excess mould which coats dry sausage. Prevalence of symptoms and sensitization to mould among these pork-butchery workers is unknown. The aim of the study was to assess the clinical, radiographic, functional, and immunological features in exposed and non-exposed workers in semi-industrial pork butcheries. Patients and methods: Symptoms, and serum precipitins against mould extracts, were studied in workers in semi-industrial pork butcheries. Of 600 workers asked to participate, 123 (20.5%) were included. Fifty-nine workers, exposed to dry (raw) sausage mould and Penicillium nalgiovense were compared with 64 non-exposed subjects, for symptoms, chest X-rays, spirometry and CO-transfer measurements. Precipitating antibodies were detected by immunoelectrophoresis and electrosyneresis. Results: Sneezing, cough, dyspnoea, nasal obstruction, headache, and discomfort were significantly more frequent in the exposed group at work and after work than in the control group (P < 0.05). The prevalence of precipitating antibodies for sausage mould was higher in the exposed group (37%) than in the non-exposed group (9%) (P < 0.01). The mean number of precipitating lines measured by electrosyneresis was higher in exposed workers than in non-exposed workers for mould extract (1.09 vs 0.28, P < 0.05) and for Penicillium nalgiovense (1.77 vs 0.33, P < 0.05). No specific X-ray opacity or lung function impairment was found in either group. Conclusions: Clinical symptoms and sensitization to Penicillium nalgiovense are frequent among workers exposed to mould during brushing in dry sausage plants. [ABSTRACT FROM AUTHOR]

Gangrenous syndrome/Degnala disease was recorded in a large number of buffaloes and cattle in Murshidabad district of West Bengal, India. Fusarium spp. had been isolated from the mouldy paddy straw which were fed to the animals. There was a reduction in the incidence of the disease following withdrawal of the mouldy paddy straw. Histopathological examination showed necrosis and loss of architectural details in the skin. [ABSTRACT FROM AUTHOR]

Thirty subjects engaged in maintenance and repair of moulds used for producing watermarks on paper were studied, along with 27 control subjects from the same paper mill. The workers were simultaneously exposed to copper, chromium, zinc, xylol, and fumes of sulphuric acid in the course of their work. The study subjects were investigated for clinical, occupational, radiological haematological and psychological details. The aforementioned combined exposure was found to be responsible for a high prevalence of symptoms pertaining to the respiratory system and higher nervous functions. Breathlessness (26.7%), expectoration (10.0%) and emphysema (10.0%) were significantly higher among the exposed subjects. The exposed subjects also showed lowered visuomotor coordination and delayed reaction to light and sound stimuli. [ABSTRACT FROM AUTHOR]

The antifungal activities of four spice decoctions (sage, wild thyme, oregano and savory) which grow wild in Turkey against six moulds ( Fusarium oxysporum f. sp. phaseoli, Macrophomina phaseoli, Botrytis cinerea, Rhizoctonia solani, Alternaria solani and Aspergillus parasiticus) were tested for fungistatic and fungicidal activity in vitro. Decoctions were added at 5% and 10% levels to 250 ml culture medium. The results showed that the decoctions investigated varied in their antifungal activity. Sage had weak activity, whilst wild thyme, oregano and savory were active against all moulds tested. All test fungi were completely inhibited by both of the concentrations of wild thyme and the 10% level of oregano decoctions in all the incubation periods. It was concluded that some decoctions may be useful as mould inhibitors at food additive levels. [ABSTRACT FROM AUTHOR]

In the basement archives of a local arts and crafts museum where the books and bookkeeping registers were handled, a woman on the museum staff had had ten attacks of fever, chill, nausea and cough during one year. The symptoms appeared at the end of the working day and disappeared after one to three days at home. No symptoms could be detected during the summer holidays. The books stored in the basement archives had earlier been stored in a small house with a leaky roof, which had led to the growth of mould on the books. When the books were handled at the museum, they were still covered with mould. The exposure measurements showed 10 CFU/m and a total of 10 microorganisms/m. [ABSTRACT FROM AUTHOR]