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3 results on '"Monika Naumowicz"'

1. The Interfacial Tension of the Lipid Membrane Formed From Lipid–Cholesterol and Lipid–Lipid Systems.

Author

Aneta Petelska, Monika Naumowicz, and Zbigniew Figaszewski

Abstract

Interfacial tension has been determined for phosphatidylcholine-cholesterol, phosphatidylcholine-phosphatidylethanolamine, and phosphatidylethanolamine-cholesterol membranes. Phosphatidylcholine (PC), phosphatidylethanolamine (PE), and cholesterol (Ch) were to be investigated, because of their presence in biological membranes. Interfacial tension values of pure components are 0.81 × 10–3 N/m, 1.67 × 10–3 N/m, and 2.36 × 10–3 N/m, respectively. The 1:1 complexes were formed during formation of the PC–Ch, PC–PE, and PE–Ch lipid membranes. The following parameters describing the complexes were determined: A3–1, the surface concentrations of the lipid membranes formed from these complexes; γ3, the interfacial tensions of such membranes and K, the stability constants of these complexes. [ABSTRACT FROM AUTHOR]

Published
2006

2. Impedance Analysis of Complex Formation Equilibria in Phosphatidylcholine Bilayers Containing Decanoic Acid or Decylamine

Author

Zbigniew A. Figaszewski, Aneta D. Petelska, and Monika Naumowicz

Subjects
Models, Molecular, Lipid Bilayers, Biophysics, Biochemistry, Decylamine, chemistry.chemical_compound, Phosphatidylcholine, Decanoic acid, Amines, Phosphatidylcholine membrane, Lipid bilayer, Electrical impedance, Original Paper, Chromatography, Chemistry, Complex formation, General Medicine, Cell Biology, Dielectric spectroscopy, Membrane, Stability constants of complexes, Dielectric Spectroscopy, Phosphatidylcholines, Physical chemistry, Electrochemical impedance spectroscopy, Decanoic Acids, Algorithms, Stoichiometry
Abstract

Bilayer lipid membranes composed of phosphatidylcholine and decanoic acid or phosphatidylcholine and decylamine were investigated using electrochemical impedance spectroscopy. Interaction between membrane components causes significant deviations from the additivity rule. Area, capacitance, and stability constant values for the complexes were calculated based on the model assuming 1:1 stoichiometry, and the model was validated by comparison of these values to experimental results. We established that phosphatidylcholine and decylamine form highly stable 1:1 complexes. In the case of decanoic acid-modified phosphatidylcholine membranes, complexes with stoichiometries other than 1:1 should be taken into consideration.

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3. Pore Formation in Lipid Bilayer Membranes made of Phosphatidylcholine and Cholesterol Followed by Means of Constant Current

Author

Monika Naumowicz and Zbigniew A. Figaszewski

Subjects
Cell Membrane Permeability, Lipid Bilayers, Chronopotentiometry, Resistance, Analytical chemistry, Biophysics, Capacitance, Model lipid bilayer, Electric Capacitance, Biochemistry, Lipid bilayer, chemistry.chemical_compound, Phosphatidylcholine, Electric Impedance, Membrane fluidity, Membrane potential, Original Paper, Chemistry, Conductance, General Medicine, Cell Biology, Cholesterol, Electroporation, Membrane, Phosphatidylcholines, Potentiometry, lipids (amino acids, peptides, and proteins), Porosity
Abstract

This paper describes the application of chronopotentiometry to lipid bilayer research. The experiments were performed on bilayer lipid membranes composed of phosphatidylcholine and cholesterol and formed using the painting technique. Chronopotentiometric (U = f(t)) measurements were used to determine the membrane capacitance, resistance, and breakdown voltage as well as pore conductance and diameter.

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