1. Tissue- and cellular localization of soybean (Glycine max L.) seed maturation protein transcripts.
- Author
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Ming-Der Shih, Mei-Hua Kao, Shu-Chin Lin, Tzu-Fu Shu, Kim-Long Hsieh, Mei-Chu Chung, Tze-Hua Tsou, Yue-Ie C. Hsing, and Jaw-Shu Hsieh
- Subjects
SOYBEAN research ,ENDOPLASMIC reticulum ,GENE expression in plants ,PLANT cells & tissues ,PEPTIDES ,IN situ hybridization ,PLANT embryology ,PLANT development ,BOTANICAL research - Abstract
Late embryogenesis abundant (LEA) genes, with abundant expression in seeds during the late developmental stages, are associated with water loss. In the present work, we characterized the spatial expression patterns of 8 soybean GmPM genes, including 7 hydrophilic LEAs and 1 maturation protein gene, in fresh or pod-dried seeds at 35 days after flowering (DAF) by in situ hybridization analysis. The transcripts of hydrophilic LEA genes were detectable in vascular tissues of the cotyledon and embryo axis (epicotyl and radicle) in fresh seeds 35 DAF. After pod-drying, the expression of these genes was greatly increased in similar tissues and was induced in other seed tissues. A maturation protein gene, GmPM5, was highly expressed in mesophyll tissues but not in vascular tissues of fresh or pod-dried seeds. Hence, LEA genes could be be highly regulated in the conductive system with changes in water content. As well, the mRNA distribution of GmPM5 and GmPM8/10, 2 messengers with endoplasmic reticulum signal peptides in the deduced proteins, significantly differed. The transcripts of these genes are therefore targeted towards different endoplasmic reticulum systems, which suggests that individual endoplasmic reticulum-targeting LEA genes may be involved in diverse translocation and subsequent protein transport systems. [ABSTRACT FROM AUTHOR]
- Published
- 2010