1. Age-dependent defective TGF-beta1 signaling in patients undergoing coronary artery bypass grafting
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Teresa Tejerina, Úrsula Medina, Pedro Molina-Sánchez, Z. Garcés, Mauricio García-Alonso, Vicente Andrés, Jorge Navarro-Dorado, Fernando Reguillo, Marta Ramajo, Santiago Redondo, and Enrique Rodríguez
- Subjects
Male ,Aging ,P27(KIP1) ,BLOOD ,Vascular smooth muscle ,medicine.medical_treatment ,Coronary Artery Disease ,Gastroenterology ,DISEASE ,Muscle, Smooth, Vascular ,Coronary artery disease ,Coronary Artery Bypass ,Cells, Cultured ,Aged, 80 and over ,Coronary disease ,PLASMA ,biology ,PROLIFERATION ,INHIBITOR ,TGF-BETA ,General Medicine ,Middle Aged ,Cardiac surgery ,medicine.anatomical_structure ,Cytokine ,Cardiothoracic surgery ,Cardiology ,Female ,Growth factors ,Cardiology and Cardiovascular Medicine ,Artery ,Signal Transduction ,Research Article ,Pulmonary and Respiratory Medicine ,Adult ,medicine.medical_specialty ,Blotting, Western ,Enzyme-Linked Immunosorbent Assay ,Transforming Growth Factor beta1 ,Internal medicine ,Proliferating Cell Nuclear Antigen ,medicine ,Humans ,Mammary Arteries ,Aged ,GROWTH-FACTOR-BETA ,business.industry ,Transforming growth factor beta ,medicine.disease ,ATHEROSCLEROSIS ,CELLS ,biology.protein ,Surgery ,business ,Abdominal surgery - Abstract
Background Transforming growth factor beta (TGF-β1) is a pleiotropic cytokine, which is deregulated in atherosclerosis; however the role of age in this process is unknown. We aimed to assess whether TGF-β1 signaling is affected by age. Methods Vascular smooth muscle cells (VSMC) were obtained from patients undergoing abdominal surgery. Levels of TGF-β1 were measured by ELISA in sera from 169 patients undergoing coronary artery bypass grafting (CABG). The p27 expression was determined by Western blot from internal mammary arteries (IMA) obtained from CABG patients (n = 13). In VSMC from these patients undergoing abdominal surgery, secretion of TGF-β1 was determined by ELISA of cell-conditioned media. Results In VSMC from aged patients we observed a lower TGF-β1 secretion, measured as TGF-β1 concentration in cell conditioned medium (p p = 0.0195). Conclusions VSMC from aged patients showed a higher degree of cellular senescence and it was associated to a lower TGF-β1 secretion and signaling.
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