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Nocardia spp., which belongs to one of the Nocardio-form filamentous bacteria, is usually surface hydrophobic and when overproduced attaches to the surface of bubbles under the action of surfactants, allowing the stable presence of foam on the surface of aeration tanks, leading to the occurrence of sludge-foaming events. Two novel phages, P69 and KYD2, were isolated from the environment, and their hosts were Nocardia transvalensis and Nocardia carnea, respectively. These two phages are Siphophages-like with long tails. An aeration tank pilot plant was constructed in the laboratory to simulate sludge foaming, and these two strains of phage were applied. Compared with the reactor not dosed with phage, the application of phage could reduce the host level in the reactor, resulting in the highest decrease in turbidity by more than 68% and sludge volume index by more than 25%. The time for surface foam disappearance was 9 h earlier than that of the control group (the group with the same concentration of Nocardia carnea but no bacteriophage applied), significantly improving water quality. The phage can effectively inhibit the propagation of Nocardia in the actual sludge-foaming event, control the sludge foaming, and improve the effluent quality. It provides a novel and relatively economical solution for controlling sludge foaming in sewage treatment plants in the future, shows that the phages have potential application value in the prevention and control of Nocardia, and provides another way to control the sludge-foaming event caused by the excessive reproduction of Nocardia in the future. [ABSTRACT FROM AUTHOR]

Edwardsiella piscicida causes significant economic losses to the aquaculture industry worldwide. Phage-based biocontrol methods are experiencing a renaissance because of the spread of drug-resistant genes and bacteria resulting from the heavy use of antibiotics. Here, we showed that the novel Edwardsiella phage EPP-1 could achieve comparable efficacy to florfenicol using a zebrafish model of Edwardsiella piscicida infection and could reduce the content of the floR resistance gene in zebrafish excreta. Specifically, phage EPP-1 inhibited bacterial growth in vitro and significantly improved the zebrafish survival rate in vivo (P = 0.0035), achieving an efficacy comparable to that of florfenicol (P = 0.2304). Notably, integrating the results of 16S rRNA sequencing, metagenomic sequencing, and qPCR, although the effects of phage EPP-1 converged with those of florfenicol in terms of the community composition and potential function of the zebrafish gut microbiota, it reduced the floR gene content in zebrafish excreta and aquaculture water. Overall, our study highlights the feasibility and safety of phage therapy for edwardsiellosis control, which has profound implications for the development of antibiotic alternatives to address the antibiotic crisis. [ABSTRACT FROM AUTHOR]

Salmonella Typhimurium, a zoonotic pathogen, causes systemic and localized infection. The emergence of drug-resistant S. Typhimurium has increased; treating bacterial infections remains challenging. Phage endolysins derived from phages have a broader spectrum of bacteriolysis and better bacteriolytic activity than phages, and are less likely to induce drug resistance than antibiotics. LysST-3, the endolysin of Salmonella phage ST-3, was chosen in our study for its high lytic activity, broad cleavage spectrum, excellent bioactivity, and moderate safety profile. LysST-3 is a promising antimicrobial agent for inhibiting the development of drug resistance in Salmonella. The aim of this study is to investigate the molecular characteristics of LysST-3 through the prediction of key amino acid sites of LysST-3 and detection of its mutants’ activity. We investigated its lytic effect on Salmonella and identified its key amino acid sites of interaction with substrate. LysST-3 may be a Ca2+, Mg2+ - dependent metalloenzyme. Its concave structure of the bottom “gripper” was found to be an important part of its amino acid active site. We identified its key sites (29P, 30T, 86D, 88 L, and 89 V) for substrate binding and activity using amino acid-targeted mutagenesis. Alterations in these sites did not affect protein secondary structure, but led to a significant reduction in the cleavage activity of the mutant proteins. Our study provides a basis for phage endolysin modification to target drug-resistant bacteria. Identifying the key amino acid site of the endolysin LysST-3 provides theoretical support for the functional modification of the endolysin and the development of subsequent effective therapeutic solutions. [ABSTRACT FROM AUTHOR]

Bacterial biofilms (BBFs) exhibit high drug resistance, antiphagocytosis, and extremely strong adhesion, and therefore can cause various diseases. They are also one of the important causes of bacterial infections. Thus, the effective removal of BBFs has attracted considerable research interest. Endolysins, which are efficient antibacterial bioactive macromolecules, have recently been receiving increasing attention. In this study, we overcame the deficiencies of endolysins via immobilization on chitosan nanoparticles (CS-NPs) by preparing LysST-3-CS-NPs using the ionic cross-linking reaction between CS-NPs and LysST-3, an endolysin purified using phage ST-3 expression. The obtained LysST-3-CS-NPs were verified and thoroughly characterized, their antimicrobial activity was investigated using microscopy, and their antibacterial efficacy on polystyrene surfaces was studied. The results obtained suggested that LysST-3-CS-NPs exhibit enhanced bactericidal properties and increased stability and can serve as reliable biocontrol agents for the prevention and treatment of Salmonella biofilm infections. [ABSTRACT FROM AUTHOR]

This study aimed to examine the direct relationship between negative family expressiveness and internalizing problems, as well as the mediating roles of emotional clarity and emotion dysregulation. A sample of Chinese families (N = 499) comprising adolescents and their parents was included. Parents completed the Family Expressiveness Questionnaire, while adolescents completed the Strengths and the Difficulties Questionnaire, the Trait Meta-Mood Scale, and the Difficulties in Emotion Regulation Scale. The results indicated that negative family expressiveness had a direct connection with internalizing problems. Moreover, negative family expressiveness had an indirect association with internalizing problems through the sequential mediating roles of emotional clarity and emotion dysregulation. These findings enhanced the understanding of the mechanism of internalizing problems and provided new perspectives for the prevention and treatment of internalizing problems among adolescents. [ABSTRACT FROM AUTHOR]

Bacterial biofilms are widespread in the environment, and bacteria in the biofilm are highly resistant to antibiotics and possess host immune defense mechanisms, which can lead to serious clinical and environmental health problems. The increasing problem of bacterial resistance caused by the irrational use of traditional antimicrobial drugs has prompted the search for better and novel antimicrobial substances. In this paper, we review the effects of phage endolysins, modified phage endolysins, and their combination with other substances on bacterial biofilms and provide an outlook on their practical applications. Phage endolysins can specifically and efficiently hydrolyze the cell walls of bacteria, causing bacterial lysis and death. Phage endolysins have shown superior bactericidal effects in vitro and in vivo, and no direct toxicity in humans has been reported to date. The properties of phage endolysins make them promising for the prevention and treatment of bacterial infections. Meanwhile, endolysins have been genetically engineered to exert a stronger scavenging effect on biological membranes when used in combination with antibiotics and drugs. Phage endolysins are powerful weapons for controlling bacterial biofilms. [ABSTRACT FROM AUTHOR]

Emotion reactivity and emotion dysregulation have been implicated in risk for suicidality. However, it remains unclear whether emotion reactivity and emotion dysregulation are predictive factors of suicide ideation, the progression from suicide ideation to suicide attempt, or both. The present study explored the relationship among emotion reactivity, emotion dysregulation, suicide ideation, and suicide attempt within a sample of Chinese undergraduates (n = 1596). According to lifetime suicidality, the current sample was divided into three groups: (a) non-ideators (no lifetime suicide ideation and no lifetime suicide attempts), (b) suicide ideators (with lifetime suicide ideation and no lifetime suicide attempts), and (c) suicide attempters (with lifetime suicide ideation and lifetime suicide attempts). Results of multinomial logistic regression suggested that compared to non-ideators, suicide ideators reported greater problems with emotion reactivity, and greater difficulties in emotion dysregulation, especially for emotion regulation strategies and emotional clarity. Compared to suicide ideators, suicide attempters reported greater difficulties in emotion dysregulation, especially for controlling impulses of negative emotions and emotional awareness. Emotion reactivity and emotion dysregulation are differentially associated with lifetime suicidality, and these findings provide preliminary guidance for suicide prevention and intervention. [ABSTRACT FROM AUTHOR]

The novel phage ST-3, capable of infecting the foodborne pathogen Salmonella Typhimurium, was isolated from wastewater. The Biological characters and genome information of ST-3 were analyzed. In the in vitro assay, the phage ST-3 with a MOI of 10 effectively inhibited the growth of Salmonella Typhimurium CGMCC 1.1174 in 6 h. The inhibitory effect of combination phage ST-3 and antibiotics was also studied, the removal rate of planktonic host exposed to ST-3 and levofloxacin hydrochloride at the same time, or to ciprofloxacin followed by ST-3, is higher than that exposed to antibiotic dosing group alone and antibiotic + phage dosing group. The phage ST-3 combined with 0.5 µg/mL levofloxacin hydrochloride resulted in the largest decrease in biofilm biomass at 54%. The phage ST-3 could be a potential agent to control Salmonella Typhimurium growth and provide instruction for use it and antibiotics together. [ABSTRACT FROM AUTHOR]

The escalation of antibiotic resistance has revitalized bacteriophage (phage) therapy. Recently, phage therapy has been gradually applied in medicine, agriculture, food, and environmental fields due to its distinctive features of high efficiency, specificity, and environmental friendliness compared to antibiotics. Likewise, phage therapy also holds great promise in controlling pathogenic bacteria in aquaculture. The application of phage therapy instead of antibiotics to eliminate pathogenic bacteria such as Vibrio, Pseudomonas, Aeromonas, and Flavobacterium and to reduce fish mortality in aquaculture has been frequently reported. In this context, the present review summarizes and analyzes the current status of phage therapy in aquaculture, focusing on the key parameters of phage application, such as phage isolation, selection, dosage, and administration modes, and introducing the strategies and methods to boost efficacy and restrain the emergence of resistance. In addition, we discussed the human safety, environmental friendliness, and techno-economic practicability of phage therapy in aquaculture. Finally, this review outlines the current challenges of phage therapy application in aquaculture from the perspectives of phage resistance, phage-mediated resistance gene transfer, and effects on the host immune system. [ABSTRACT FROM AUTHOR]

In the context of widespread bacterial contamination and the endless emergence of antibiotic-resistant bacteria, more effective ways to control pathogen infection are urgently needed. Phages become potential bactericidal agents due to their bactericidal specificity and not easy resistance to bacteria. But an important factor limiting its development is the lack of phage species. Therefore, the isolation of more new phages and studying their biological and genomic characteristics is of great significance for subsequent applications. So, in this study, SGF3, a Microviridae phage, which has shown lytic activity against Shigella flexneri, was isolated, purified, and characterized. Morphological and phylogenetic analyses identified it as a phiX174 species belonging to the Microviridae family. The latent period of phage SGF3 was 20 min, with an average burst size of approximately 7.1. Host spectrum experiments indicated its strong host specificity. Furthermore, the biofilm removal efficiency was increased by 20%-25% when SGF3 was coupled with other phages. In conclusion, the phage SGF3 found in this study was a lytic phage belonging to the Microviral family, and could be added as an auxiliary material in the phage cocktail. Studies of its characteristics and bactericidal properties had enriched the germplasm resources of microphages, provided more potential material in fighting against emerging and existing multidrug-resistant bacteria. [ABSTRACT FROM AUTHOR]

Due to the COVID-19 pandemic in early 2020, large-scale industrial production has been stagnant and reduced, the urban air quality has been greatly improved. It provided an excellent opportunity to explore the effects of air pollutants on the sensitization of pollen allergen proteins in the environment. Platanus pollen grains sampled in the spring of 2019 and 2020 were used for detailed characterization and analysis. Scanning electron microscopy, Fourier transform infrared, X-ray spectroscopy (XPS), trypan blue staining, and western blot analysis were employed to characterize Platanus pollen protein released from pollen grains. Our data showed that the viability of the pollen grains in 2019 was lower compared that in 2020, and the pollen grains collected in 2019 had a higher absorption peak of protein functional groups. The XPS spectra assay result demonstrated that the binding energy of the high-resolution components had not variation on the surface of pollen grains, but relative content of nitrogen and peptide chain in the pollen grains sampled in 2019 were higher than in 2020. These results suggested that more protein in the pollen grains was released onto the surface of pollen grains. In addition, western blot assay showed that the expression of Pla a3 protein in pollen grains sampled in 2019 was significantly higher than that in 2020, revealing that air pollutants could enhance the expression of Pla a3 proteins in Platanus pollen. [ABSTRACT FROM AUTHOR]

Arid lands such as deserts cover more than 35% of the Earth's land surface. Concerns regarding ground-level O3 pollution have been widely addressed in many regions in China, but the behavior of the O3 cycles in the desert atmosphere remains poorly understood. An 8-year observation was conducted to investigate the long-term temporal O3 variations in the atmosphere of the Taklimakan Desert, western China. The results showed that the diurnal and seasonal variations of O3 were mainly influenced by natural processes such as temperature, leading to a different temporal pattern compared with other Chinese regions under the intensive influence of anthropogenic activities. Moreover, the long-term pattern indicates that there exists decoupling trends of O3 variations between our study site (i.e., decreasing trend) and the developed regions in China (i.e., increasing trend). Knowledge of the temporal O3 patterns could therefore provide crucial insights for future effective O3 control strategies in China. [ABSTRACT FROM AUTHOR]

Biological wastewater treatment (BWT) is currently the most widely applied approach for treating wastewater. The performance of BWT systems depends on the complex microbial communities they support. Although bacteriophages (phages), which are the viruses that infect prokaryotes, are recognized as the most abundant life entities, understanding of their ecological roles in BWT systems remains limited. Here, we review recent progress in phage-associated researches in BWT systems, including the interactions between phage and host, polyvalent phages, the influence of phage activity on BWT performance, and the potential applications of phage-based control for sludge bulking/foaming and pathogens. The challenges and perspectives of phage ecology are also outlined, which are expected to provide implications for future research and applications. Key points • Phage-host interactions in BWT systems are summarized • Impacts of phage activities on BWT performance • Potential applications of phages in BWT systems [ABSTRACT FROM AUTHOR]

The genome sequence, morphology, and genetic features of a novel phage, named SSE1, is reported here. Phage SSE1 that infects Shigella dysenteriae (China General Microbiological Culture Collection Center number: 1.1869) was isolated from the aeration tank water of a sewage treatment plant. SSE1 showed morphological features associated with those of phages in Myoviridae. The whole genome sequence of phage SSE1 is composed of 169,744 bp with the GC content of 37.51%. The double-stranded DNA of SSE1 contains 270 open reading frameworks (ORFs). Phylogenetically, phage SSE1 showed a stronger homology (whole genome and terminase large subunit protein sequence) to Escherichia phages than other Shigella phages in the NCBI database, but SSE1 did not infect Escherichia stains. This indicates that phage SSE1 should be a novel phage infecting Shigella dysenteriae. Besides, the result of this study provided a new idea for phage therapy. SSE1 may become a candidate for potential therapy against Shigella dysenteriae infection in clinical applications. [ABSTRACT FROM AUTHOR]

Humic-like substances (HULIS), the most ubiquitous class of water-soluble organic compounds in the atmosphere could enhance the generation of reactive oxygen species (ROS), and play a significant role in impacting aerosol chemistry and health effects. In this study, twenty-three PM2.5 samples were collected in the atmosphere of suburban Shanghai from November 29 to December 17, 2015, and March 17 to April 30, 2016, during haze and non-haze days. The mean concentrations of HULIS in spring both in haze and non-haze days (2.34 ± 0.70 µg/m3 and 1.94 ± 0.88 µg/m3) were relatively higher than in that of winter (1.93 ± 0.95 µg/m3 and 1.31 ± 0.28 µg/m3). The ammonium, sulfate, and nitrate are the dominant ionic species in both winter and spring during haze days in suburban Shanghai. Correlation results revealed that HULIS formation was highly associated with the biomass burning (K) and secondary aerosols formation (SIA: NH4+, SO42−, NO3−) and also well-correlated with F− and ca.2+ ions, crustal elements (Al and Fe) and anthropogenic pollution metals (As, Se, Rb, Sr, and Pb), suggesting that HULIS-C formation might be from biomass burning and secondary aerosol processes and also mixed formation (marine, crustal and industrial emissions) sources. From the coinciding results of the clustering analysis and weighted-CWT model, the principal potential source regions were the short transports from the Yangtze River Delta (YRD) regions, local regions, marine areas (the Bohai Sea, the Yellow Sea, the East China Sea) and also the long-range transports from northwestern in those seasons. [ABSTRACT FROM AUTHOR]

Salmonella is a significant food-borne pathogen that infects a large number of people worldwide. In this study, a lytic bacteriophage vB_SenS_SE1 capable of infecting Salmonella is isolated from municipal wastewater in Beijing, and its biological and genomic features are analyzed. Transmission electron micrograph shows that vB_SenS_SE1 is likely a Siphoviridae virus, with an icosahedral head and a long non-contracted tail. The stability test in vitro reveals that it is stable at 4–50 °C and pH 4–12. Based on the one-step growth curve, vB_SenS_SE1 has a 60-min exponential phase and a low burst size (19 PFU per cell). Bioinformatics analysis reveals that vB_SenS_SE1 consists of a circular, double-stranded DNA molecule of 40,987 bp with a GC content of 51.2%. Its genome carries 63 predicted open reading frames (orfs), with 22 orfs encoding known proteins. Phylogenetic analysis of the large terminase subunit shows that vB_SenS_SE1 exhibits strong homology to Salmonella phage St161, St162, VSiP, and FSL SP-031. The CoreGenes analysis shows that it is a member of the virus genus Cornellvirus. The features of phage vB_SenS_SE1 suggest that it has the potential to be an agent to control Salmonella. [ABSTRACT FROM AUTHOR]

A virulent phage, named ST20, infecting Escherichia coli O165:H8 was isolated from wastewater and subjected to genomic sequencing using the Illumina HiSeq system. Genomic analysis revealed that it contains double-stranded DNA, and its complete genome consists of 44,517 nucleotides with an average GC content of 50.81%. Morphological observations showed that phage ST20 belongs to the order Caudovirales and the family Siphoviridae due to its characteristic icosahedral capsid and a long noncontractile tail. This phage was further characterized by one-step growth curve analysis and measurement of its stability at 4 °C. The study has implications for the development of potential biocontrol agents. [ABSTRACT FROM AUTHOR]

Soil and the atmospheric conditions are important factors that affect wind speed threshold of surface dust emissions. Based on the observed data of surface dust emissions in the Taklimakan Desert collected from March 2008 to February 2018, the effects of soil moisture, air humidity (vapor pressure), and air temperature on wind speed threshold were analyzed in this study. The results showed that the accumulated time of dust emissions over the decade was 3609.8 h, thereby accounting for 4.1% of the total observation time. In addition, the duration of dust emission in the four seasons were consistent with the pattern of summer > spring > autumn > winter. When the soil moisture is above 3.0–4.0%, the wind speed threshold for dust emission increases with increasing soil moisture. When the vapor pressure is above 10–15 kPa, the wind speed threshold for dust emission increases with increasing vapor pressure. There was a negative correlation between air temperature and the wind speed threshold for dust emission. When the air temperature was higher than 0.0 °C, the soil moisture decreased with increasing air temperature, and the wind speed threshold for dust emission was lower. [ABSTRACT FROM AUTHOR]

More and more virulent phages that are fundamental materials for phage therapy have been isolated, characterized and categorized on GenBank. Phage ST31 infecting Escherichia coli H21 was isolated from wastewater and sequenced using an Illumina Hiseq system. Opening reading frames were identified using PHASTER and predicted using BLASTp analysis. Genomic analyses revealed that this was a virulent phage containing a circular double-stranded DNA and that the complete genome consisted of 39,693 nucleotides with an average GC content of 49.98 %. This study may provide possible alternative materials for phage therapy. [ABSTRACT FROM AUTHOR]

A field experiment was conducted from 2 May 2010 to 1 May 2012 in the Gurbantunggut Desert, the second largest desert in China, to investigate saltation activity and its threshold velocity, and their relations with atmospheric and soil conditions. The results showed that saltation activity occurred more frequently during 08:00-20:00 Local Standard Time in spring and summer, with air temperatures between 20.0 and 29.0 °C, water vapor pressures between 0.6 and 0.9 kPa, soil temperatures between 25.0 and 30.0 °C, and a soil moisture lower than 0.04 m/m. At 2 m height, the saltation threshold velocity varied between 11.1 and 13.9 m/s, with a mean of 12.5 m/s. Threshold velocity showed clear seasonal variations in the following sequence: spring (11.7 m/s) < autumn (12.7 m/s) < summer (13.6 m/s). Affected by soil conditions, aeolian sand transport was weak, with an average annual aeolian sand that transported across a section (1.0 m × 2.0 m) of less than 6.0 kg. [ABSTRACT FROM AUTHOR]

The threshold velocity is an indicator of a soil's susceptibility to saltation activity and is also an important parameter in dust emission models. In this study, the saltation activity, atmospheric conditions, and soil conditions were measured from 1 August 2008 to 31 July 2009 in the Taklimakan Desert, China. the threshold velocity was estimated using the Gaussian time fraction equivalence method. At 2 m height, the 1-min averaged threshold velocity varied between 3.5 and 10.9 m/s, with a mean of 5.9 m/s. Threshold velocities varying between 4.5 and 7.5 m/s accounted for about 91.4% of all measurements. The average threshold velocity displayed clear seasonal variations in the following sequence: winter (5.1 m/s) < autumn (5.8 m/s) < spring (6.1 m/s) < summer (6.5 m/s). A regression equation of threshold velocity was established based on the relations between daily mean threshold velocity and air temperature, specific humidity, and soil volumetric moisture content. High or moderate positive correlations were found between threshold velocity and air temperature, specific humidity, and soil volumetric moisture content (air temperature r = 0.75; specific humidity r = 0.59; and soil volumetric moisture content r = 0.55; sample size = 251). In the study area, the observed horizontal dust flux was 4198.0 kg/m during the whole period of observation, while the horizontal dust flux calculated using the threshold velocity from the regression equation was 4675.6 kg/m. The correlation coefficient between the calculated result and the observations was 0.91. These results indicate that atmospheric and soil conditions should not be neglected in parameterization schemes for threshold velocity. [ABSTRACT FROM AUTHOR]

Monitoring, modeling and predicting the formation and movement of dust storms across the global deserts has drawn great attention in recent decades. Nevertheless, the scarcity of real-time observations of the wind-driven emission, transport and deposition of dusts has severely impeded progress in this area. In this study, we report an observational analysis of sand-dust storm samples collected at seven vertical levels from an 80-m-high flux tower located in the hinterland of the great Taklamakan Desert for ten sand-dust storm events that occurred during 2008-2010. We analyzed the vertical distribution of sandstorm particle grain sizes and horizontal sand-dust sediment fluxes from the near surface up to 80 m high in this extremely harsh but highly representative environment. The results showed that the average sandstorm grain size was in the range of 70 to 85 μm. With the natural presence of sand dunes and valleys, the horizontal dust flux appeared to increase with height within the lower surface layer, but was almost invariant above 32 m. The average flux values varied within the range of 8 to 14 kg m and the vertical distribution was dominated by the wind speed in the boundary layer. The dominant dust particle size was PM and below, which on average accounted for 60-80 % of the samples collected, with 0.9-2.5 % for PM, 3.5-7.0 % for PM, 5.0-14.0 % for PM and 20.0-40.0 % for PM. The observations suggested that on average the sand-dust vertical flux potential is about 0.29 kg m from the top of the 80 m tower to the upper planetary boundary layer and free atmosphere through the transport of particles smaller than PM. Some of our results differed from previous measurements from other desert surfaces and laboratory wind-dust experiments, and therefore provide valuable observations to support further improvement of modeling of sandstorms across different natural environmental conditions. [ABSTRACT FROM AUTHOR]

Sludge bulking, a notorious microbial issue in activated sludge plants, is always accompanied by dramatic changes in the bacterial community. Despite large numbers of phages in sludge systems, their responses to sludge bulking and phage-host associations during bulking are unknown. In this study, high-throughput sequencing of viral metagenomes and bacterial 16S rRNA genes were employed to characterize viral and bacterial communities in a sludge plant under different sludge conditions (sludge volume index (SVI) of 180, 132, and 73 ml/g). Bulking sludges (SVI > 125 ml/g) taken about 10 months apart exhibited similar bacterial and viral composition. This reflects ecological resilience of the sludge microbial community and indicates that changes in viral and bacterial populations correlate closely with each other. Overgrowth of ' Candidatus Microthrix parvicella' led to filamentous bulking, but few corresponding viral genotypes were identified. In contrast, sludge viromes were characterized by numerous contigs associated with ' Candidatus Accumulibacter phosphatis,' suggesting an abundance of corresponding phages in the sludge viral community. Notably, while nitrifiers (mainly Nitrosomonadaceae and Nitrospiraceae) declined significantly along with sludge bulking, their corresponding viral contigs were identified more frequently and with greater abundance in the bulking viromes, implying that phage-mediated lysis might contribute to the loss of autotrophic nitrifiers under bulking conditions. [ABSTRACT FROM AUTHOR]

Purpose: The purpose of this study was to evaluate the diagnosis and surgical management of insulinomas in a single-center setting. Methods: Demographic details, clinical presentation, preoperative diagnosis, surgical strategy, and outcomes of 33 consecutive patients who underwent surgery for insulinoma were analyzed retrospectively. Results: The median time from the first onset of symptoms to diagnosis was 24 months (range 3 days to 50 years). All cases presented with Whipple's triad and had a fasting insulin to glucose ratio higher than 0.33. The preoperative detection rates of transabdominal ultrasonography, CT, MRI, and EUS were 22 % (2/9), 72 % (23/32), 75 % (9/12), and 80 % (4/5), respectively. Intraoperative manual palpation localized all cases. Enucleation was performed in 58 % of cases (19/33), partial pancreatic resection in 39 % (13/33), and enucleation plus partial resection in 3 % (1/33). Pancreatic fistula was the most common complication and occurred in 15 patients (45 %), including seven grade A, five grade B, and three grade C fistulas. There was no mortality. When compared with partial pancreatectomy, enucleation held a significant advantage in operative time and operative bleeding, with no significant differences in demographic data and postoperative complications. During a median follow-up period of 32 months, two patients were lost to follow-up and the remaining 31 patients were without evidence of recurrence. Conclusions: A fasting insulin release index larger than 0.3 is a reliable indicator for the diagnosis of insulinoma. Intraoperative palpation by an experienced surgeon can effectively complement an uncertain preoperative localization. Compared with partial pancreatic resection, enucleation showed significant benefit in terms of intraoperative blood loss and operation time. [ABSTRACT FROM AUTHOR]

A novel virulent phage PE3-1 against E. coli O153 was isolated from an aeration tank in a wastewater treatment plant. Transmission electron microscopy images showed that phage PE3-1 had an icosahedral head and a short tail, which revealed that it was a member of the family Podoviridae of the order Caudovirales. The complete PE3-1 genome consisted of 39,093 bp and was a linear double-stranded DNA with an average GC content of 49.93 %. Phage PE3-1 showed homology to the T7-like phages in 48 open reading frames (ORFs), but it differed from previously reported E .coli phages in morphology and bioinformatics analysis. This indicated that phage PE3-1 is a new member of the genus T7 virus. [ABSTRACT FROM AUTHOR]

Horizontal dust fluxes were simulated with two different dust emission schemes developed by Marticorena and Shao (hereinafter referred to as the M scheme, S scheme, and S scheme corrections), based on field experiments over a bare desert surface and a vegetated desert surface from May 19 to June 18, 2010 in Xinjiang, China. The M scheme produced a much higher dust emission than the S schemes over different surface conditions, with the emission being about 4 times larger than that produced by the S schemes over the bare desert, and 3 to 200 times larger over the vegetated surface. Compared to observations, the missing report rate of wind erosion events was about 30 % for the S schemes and about 10 % for the M scheme over the bare desert surface, while all schemes had a false alarm rate of wind erosion events over the vegetated desert surface. The total dust emission from the bare desert surface during the study period was 674.4, 551.5, 595.2, and 2995.8 kg/m for observation, the S scheme, S scheme correction 2, and M scheme, respectively. Total dust emission from the vegetated desert surface was 1.6, 0, 55.5, 0.9, and 227.7 kg/m for observation, the S scheme, S scheme correction 1, S scheme correction 2, and M scheme, respectively. [ABSTRACT FROM AUTHOR]

The Taklimakan Desert is the source of most blowing dust events in China. However, previous studies of sandstorms in this region have not included data from the inner desert because of the difficulty in making observations in this area. In this study, the spatial and temporal variations of blowing dust events, including sandstorms and blowing sand, and its relations with climatic parameters in the Taklimakan Desert were analyzed using data from ten desert-edge meteorological stations during 1961 to 2010 and two inner-desert meteorological stations during 1988 to 1990, 1996 to 2010, and 1992 to 2010. The results identified two regions (Pishan-Hotan-Minfeng and Xiaotang-Tazhong) where blowing dust events occur on average more than 80 days per year. The regions with the highest occurrence of sandstorms, blowing sand, and blowing dust events were different, with sandstorms centered in the north of the desert (Xiaotang, 46.9 days), whereas the central location for blowing sand (Pishan, 86.4 days) and blowing dust events (Minfeng, 113.5 days) activity was located at the southwestern and southern edges of the desert, respectively. The occurrence of sandstorms generally decreased from 1961 to 2010, while the occurrence of blowing sand increased from 1961 to 1979 and then generally decreased. The temporal variation of blowing dust events was mainly affected by the occurrence of strong wind and daily temperature, with average correlation coefficients of 0.46 and −0.41 for these variables across the whole desert. [ABSTRACT FROM AUTHOR]

The Lopingian is one of the fastest rising periods of seawater strontium isotopic ratios (Sr/Sr) in earth history, and its mechanisms and increasing rates of the Sr/Sr evolution were still disputed widely. These disputations among researchers were caused mainly by timeframe selection (sections' thickness or data of radiometric ages), and different stratigraphic boundaries and un-upmost dated ages. This paper examined published Sr/Sr data of the Lopingian, and projected them on timescales based on evolutionary and age constrained conodonts fossils. Sr/Sr evolution vs fossil constraining timescales was re-established in this period. This research suggests: (1) Sr/Sr excursion projects on fossil zones can truly support Sr/Sr evolutionary pattern in the period; (2) Sr/Sr evolution provides a new approach for stratigraphic research of marine carbonate sections in lieu of biostratigraphic data; (3) Sr/Sr stratigraphy works on marine carbonate sections of different sedimentation rates even between different basins; (4) the Sr/Sr data and its shift was dependent on samples materials and chemical treatment methods; (5) the increasing rate of marine water Sr/Sr in the Late Permian is suggested as 5.4×10/Ma or slightly lower; (6) sedimentation age and its Sr/Sr of the Lopingian marine carbonate suggested as: D=259-( R−0.70695)/5.4×10±1 Ma. [ABSTRACT FROM AUTHOR]

Introduction: Laminoplasty and skip laminectomy are two specific posterior surgical approaches for multilevel cervical spondylotic myelopathy. The objective of this study was to perform a systematic review comparing the clinical results and complications of laminoplasty and skip laminectomy in the treatment of multilevel cervical spondylotic myelopathy. Materials and methods: We reviewed and analyzed papers published from January 1969 to December 2012 through the Mediline, Embase, Cochrane review library, and other databases regarding the comparison between laminoplasty and skip laminectomy for multilevel cervical spondylotic myelopathy. Results: One randomized controlled trial and three non-randomized controlled trials were included in this systematic review. In three studies, the preoperative and postoperative JOA score was similar in both laminoplasty and skip laminectomy groups. In addition, for recovery rate, there was no significant difference between the groups. One study reported that, regarding SF12 scores, there was no significant difference in physical health and mental health after surgery. However, regarding cervical pain, the skip laminectomy group was better than the laminoplasty group significantly. No difference was presented in postoperative ROM and the cervical lordosis between the groups. But the ROM % (post/pre) was reported to be significantly better in the skip laminectomy group in three studies. Less blood loss and shorter operation time were observed in skip laminectomy rather than laminoplasty. Conclusions: Based on the results above, the skip laminectomy group presented better outcomes in a variety of aspects: ROM % (post/pre), complication rate, surgical trauma, etc. However, as limited study samples were included in the paper, a claim of superiority of the two approaches could not be justified. Further studies are required on the comparison between laminoplasty and skip laminectomy. [ABSTRACT FROM AUTHOR]

Black carbon concentration and weather data were online monitored continuously from March 2008 to February 2009 at the Akedala regional atmosphere station in the arid region of Central Asia. We present the daily, monthly and seasonal variations of BC concentration in the atmosphere and discuss the possible emission sources. Black carbon concentration in this region varies in the range of 43.7-1,559.2 ng/m. A remarkable seasonal variation of BC in the aerosol was observed in the order of winter > spring > autumn > summer. The peak value of BC appeared at 10:00-13:00 while the lowest one at 7:00-9:00 each day. Air masses backward trajectories show the potential emission sources in the northwest from spring to autumn. Through back trajectory also revealed that BC in winter might be attributed to the emission from the anthropogenic activities, including domestic heating, cooking, combustion of oil and natural gas, and the medium-range transport from those cities in northern slope of Tianshan Mountains and Siberia. Some BC aerosols from the arid region of Central Asia were transported to the Pacific Ocean by the Westerlies. [ABSTRACT FROM AUTHOR]

Dust emission by wind erosion in surface is a serious problem in many arid regions around the world, and it is harmful to the ecological environment, human health, and social economy. To monitor the characteristics of saltation activity and to calculate the threshold wind velocity and sediment discharge under field conditions have significance on the research of dust emission by wind erosion. Therefore, a field experiment was conducted over the flat sand in the hinterland of the Taklimakan Desert. One sampling system was installed on the flat sand surface at Tazhong, consisting of a meteorological tower with a height of 2 m, a piezoelectric saltation sensor (Sensit), and a Big Spring Number Eight (BSNE) sampler station. Occurrence of saltation activity was recorded every second using the Sensit. Each BSNE station consisted of five BSNE samplers with the lowest sampler at 0.05 m and the highest sampler at 1.0 m above the soil surface. Sediment was collected from the samplers every 24 h. It is found that saltation activity was detected for only 21.5% of the hours measured, and the longest period of saltation activity occurring continuously was not longer than 5 min under the field conditions. The threshold wind velocity was variable, its minimum value was 4.9 m s, the maximum value was 9.2 m , and the average value was 7.0 m s. The threshold wind velocity presented a positive linear increase during the measurement period. The observation site had a sediment discharge of 82.1 kg m over a period of 24 h. Based on hourly saltation counts, hourly sediment discharge was estimated. Overall, there was no obvious linear or other functional relationship between the hourly sediment discharge and wind velocity. The results show that the changes of sediment discharge do not quite depend on wind velocity. [ABSTRACT FROM AUTHOR]

Up to now, there are many homology search algorithms that have been investigated and studied. However, a good classification method and a comprehensive comparison for these algorithms are absent. This is especially true for index based homology search algorithms. The paper briefly introduces main index construction methods. According to index construction methods, index based homology search algorithms are classified into three categories, i.e., length based index ones, transformation based index ones, and their combination. Based on the classification, the characteristics of the currently popular index based homology search algorithms are compared and analyzed. At the same time, several promising and new index techniques are also discussed. As a whole, the paper provides a survey on index based homology search algorithms. [ABSTRACT FROM AUTHOR]

The architecture of a BioAccel (internal code) chip for RNA secondary structure prediction is described in the letter. The system is based on a BioBus (internal code), whose distinguishing features are: Two separated control and data channels, and a slave-associated arbitration scheme. Two reference systems based on the AMBA AHB bus and Coreconnect bus are introduced to evaluate the performance of the system. The simulation results are attractive. The average communication bandwidth of the chip is increased at severalfold, and the read and write latencies are reduced about 40 percent. [ABSTRACT FROM AUTHOR]

This paper presents a new method for detection of edges in digital angiographic images. It is found that variances of local regions across edges of images are statistically different from that of those where no edge is crossed. This difference can be utilized for the detection of edges of angiographic images. An algorithm based on local variance is proposed. As a result, the edge-detection algorithm is not sensitive to noise and low-level textures of images. A computer program based on the new algorithm has been developed and used by several hospitals. [ABSTRACT FROM AUTHOR]