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To study the expressions and clinical significance of uPA and V-ATPase mRNA in human hepatocellular carcinoma. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expressions of uPA and V-ATPase mRNA in 45 specimens of hepatocellular carcinoma (HCC), their para-neoplastic tissues and 7 parahepatic hemangioma tissues for controls. The diagnosis for all specimens was made by pathologists. The positive rates of uPA mRNA in HCC, para-neoplastic tissues and the controls were 100%, 86.44% and 28.57% respectively, and there were significant differences among three groups ( P < 0.05). The positive rates of V-ATPase mRNA in HCC, para-neoplastic tissues and the controls were 100%, 86.67% and 57.14% respectively, and there were also significant differences among three groups ( P < 0.05). In addition, the positive rates of uPA and V-ATP mRNA in HCC were significantly correlated with portal vein cancer thrombosis and remote metastasis ( P < 0.01). However, the positive rates had no correlation to cancer size or pathological stage, serum AFP lever and HBV infection ( P > 0.05). The high expressions of uPA and V-ATPase mRNA in HCC might be related to its growth, invasion and metastasis. [ABSTRACT FROM AUTHOR]

Objective: To evaluate the association of Glutathione S-transferase (GST) M1 and T1 genetic polymorphisms and susceptibility to nasopharyngeal carcinoma (NPC) in a high risk area of Guangxi Zhuang Autonomous Region (province), Southwest of China. Methods: A case-control study was conducted to investigate the genetic polymorphisms of these enzymes (GSTM1 and GSTT1 null genotypes). A total of 127 NPC cases and 207 controls were recruited. Results: GSTM1 and GSTT1 null genotype frequencies were higher among NPC patients at a level of statistical significance (P<0.005; P<0.001 respectively), and both GSTM1 and GSTT1 null genotype were even more significant (P<0.001). Conclusion: NPC is the most common cancer in Guangxi. GST enzymes are involved in the detoxification of many environmental carcinogens. Homozygous deletions of GSTM1 and GSTT1 have been associated with several types of cancer. The risk to develop NPC has been associated with environmental factors such as cigarette smoking and EB virus infection. The present results indicate that the GSTM1 and GSTT1 deletion polymorphisms are associated with an increase risk of susceptibility to NPC, and both detoxific enzyme genes deletion is more important than a single gene deletion for the susceptibility to NPC. [ABSTRACT FROM AUTHOR]