1. Bacillus subtilis B10 improved antioxidant capacity, immunity, and lipid metabolism of Chinese mitten crab (Eriocheir sinensis) during hoarding time.
- Author
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Jin, Qian, Xu, Shujie, Wang, Qi, Wang, Fei, Li, Xiang, Wang, Xiangcheng, and Li, Weifen
- Subjects
CHINESE mitten crab ,AGRICULTURE ,GENE expression ,OXIDANT status ,BACILLUS subtilis - Abstract
Farmers usually prefer to sell Chinese mitten crabs (Eriocheir sinensis) until around the Spring Festival for greater economic benefits. However, the hoarding stages in winter and cold chain logistics would reduce the vitality of crabs, which would affect the economic benefits. Therefore, this experiment investigated the effects of adding Bacillus subtilis B10 on their health and vitality status after transportation, as well as the immunity and lipid metabolism in cold. The results showed that adding 1 × 10
9 CFU/kg B. subtilis B10 in feed could improve the vitality of crabs and reduce feet breakage caused by transportation. In male crabs, it could promote growth, and improve antioxidant capacity by increasing the expression of antioxidant gene including SOD and PRX1, increasing the activity of CuZn-SOD and T-SOD, and regulate fat metabolism by decreasing the relative expression of FAD6 and FAD9 thus adapting to environmental changes. In female crabs, it could not promote growth, but it could improve antioxidant capacity by increasing the activity of T-AOC, CuZn-SOD, and T-SOD, and regulate the expression of immune-related and apoptosis-related genes including CASP1, AKT, and AQP and regulate fat metabolism by decreasing the relative expression of FAD6, FAD9, and ELOVL6, thus adapting to environmental changes. This experiment provided a reference basis for reducing economic losses in the hoarding stage and transportation, promoting breeding methods and nutritional requirements of single-sex farming models, and application of probiotics in the field of crab farming. [ABSTRACT FROM AUTHOR]- Published
- 2024
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