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Cancer cells often have unstable genomes and increased centrosome and chromosome numbers, which are an important part of malignant transformation in the most recent model of tumorigenesis. However, very little is known about divisional failures in cancer cells that may lead to chromosomal and centrosomal amplifications. In this study, we show that cancer cells often failed at cytokinesis because of decreased phosphorylation of the myosin regulatory light chain (MLC), a key regulatory component of cortical contraction during division. Reduced MLC phosphorylation was associated with high expression of myosin phosphatase and/or reduced myosin light-chain kinase levels. Furthermore, expression of phosphomimetic MLC largely prevented cytokinesis failure in the tested cancer cells. When myosin light-chain phosphorylation was restored to normal levels by phosphatase knockdown, multinucleation and multipolar mitosis were markedly reduced, resulting in enhanced genome stabilization. Furthermore, both overexpression of myosin phosphatase or inhibition of the myosin light-chain kinase in nonmalignant cells could recapitulate some of the mitotic defects of cancer cells, including multinucleation and multipolar spindles, indicating that these changes are sufficient to reproduce the cytokinesis failures we see in cancer cells. These results for the first time define the molecular defects leading to divisional failure in cancer cells. [ABSTRACT FROM AUTHOR]

Experimental measurements and DFT calculations reveal that, as expected, CO adsorbs most strongly at the step edge of Pt{211}, with similar adsorption energies for bridged and atop CO on the step. Terrace sites are significantly less stable. In the light of our results, we attempt to provide explanations for previous disagreement between experiment and theory. [ABSTRACT FROM AUTHOR]

Annihilating polynomials for quadratic forms in the Witt ring are obtained via an étale algebra interpretation of the Burnside ring together with a homomorphism to the Witt ring. [ABSTRACT FROM AUTHOR]

The involutions in this paper are algebra anti-automorphisms of period two. Involutions on endomorphism algebras of finite-dimensional vector spaces are adjoint to symmetric or skew-symmetric bilinear forms, or to hermitian forms. Analogues of the classical invariants of quadratic forms (discriminant, Clifford algebra, signature) have been defined for arbitrary central simple algebras with involution. In this paper it is shown that over certain fields these invariants are sufficient to classify involutions up to conjugation. For algebras of low degree a classification is obtained over an arbitrary field. [ABSTRACT FROM AUTHOR]

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