1. Cell proliferation detected using [18F]FLT PET/CT as an early marker of abdominal aortic aneurysm
- Author
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Gandhi, R, Cawthorne, C, Craggs, LJL, Wright, JD, Domarkas, J, He, P, Koch-Paszkowski, J, Shires, M, Scarsbrook, AF, Archibald, SJ, Tsoumpas, C, and Bailey, MA
- Subjects
cardiovascular system - Abstract
Background: Abdominal aortic aneurysm (AAA) is a focal aortic dilatation progressing towards rupture. Non-invasive AAA-associated cell proliferation biomarkers are not yet established. We investigated the feasibility of the cell proliferation radiotracer, fluorine-18-fluorothymidine ([18F]FLT) with positron emission tomography/computed tomography (PET/CT) in a progressive pre-clinical AAA model (angiotensin II, AngII infusion). Methods and Results: Fourteen-week-old apolipoprotein E-knockout (ApoE−/−) mice received saline or AngII via osmotic mini-pumps for 14 (n = 7 and 5, respectively) or 28 (n = 3 and 4, respectively) days and underwent 90-minute dynamic [18F]FLT PET/CT. Organs were harvested from independent cohorts for gamma counting, ultrasound scanning, and western blotting. [18F]FLT uptake was significantly greater in 14- (n = 5) and 28-day (n = 3) AAA than in saline control aortae (n = 5) (P
- Published
- 2021