1. Nanozyme-based dual-signal sensing system for colorimetric and photothermal detection of AChE activity in the blood of liver-injured mice.
- Author
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He, Chang, Ke, Zhenyi, Liu, Kai, Peng, Jiasheng, Yang, Qinghui, Wang, Lixiang, Feng, Guangfu, and Fang, Jun
- Subjects
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NEURAL transmission , *MICE , *POINT-of-care testing , *PHOTOTHERMAL effect , *LIVER injuries , *LIVER enzymes - Abstract
Acetylcholinesterase (AChE), a crucial enzyme related to liver function, is involved in numerous physiological processes such as neurotransmission and muscular contraction. The currently reported techniques for detecting AChE mainly rely on a single signal output, limiting their high-accuracy quantification. The few reported dual-signal assays are challenging to implement in dual-signal point-of-care testing (POCT) because of the need for large instruments, costly modifications, and trained operators. Herein, we report a colorimetric and photothermal dual-signal POCT sensing platform based on CeO2-TMB (3,3′,5,5′-tetramethylbenzidine) for the visualization of AChE activity in liver-injured mice. The method compensates for the false positives of a single signal and realizes the rapid, low-cost portable detection of AChE. More importantly, the CeO2-TMB sensing platform enables the diagnosis of liver injury and provides an effective tool for studying liver disease in basic medicine and clinical applications. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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