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Absolute grand total cross section for electron scattering from CO 2 is obtained in the energy range from 20 eV down to 9 meV with a very narrow electron energy width of 11 meV using the threshold-photoelectron source employing synchrotron radiation. Although the obtained grand total cross section shows the general features of the cross-section behavior known for the low-energy electron scattering from CO 2 , namely the marked rise below 2 eV and the resonance enhancement at about 4 eV, several new features were revealed, especially at the 2 Π u resonance. The energy position for the maxima of the grand total cross section at the 2 Π u resonance appeared to be lower than that has been known. Also found are clear oscillatory structures resolved on the broad enhancement of the grand total cross section at around the 2 Π u resonance. The present measurement also revealed a threshold feature due to the vibrational excitation of asymmetric stretch mode in the grand total cross section. The vibrational excitation cross section for asymmetric stretch mode at the threshold is estimated from the feature. [ABSTRACT FROM AUTHOR]

Physical studies of electric dipole excitations in atomic nuclei e.g. the structure of pygmy dipole resonances and isovector giant dipole resonances are attracting much attention recently. In this article, we describe a technical development in the coincidence measurement of the excitation processes with the Grand Raiden high-resolution magnetic spectrometer and the γ -decay processes by the CAGRA and SC γ LLA efficient γ -detector arrays at the Research Center for Nuclear Physics at Osaka University. Specifically, we describe how we developed a new beamline (GRAF), for γ detection at the target position by placing the spectrometer at an angle of 4. 5 - 19. 0 ∘ and by transporting the primary beam to a well-shielded beam dump. Experimental conditions and representative data are shown for each of the two γ -detector arrays. [ABSTRACT FROM AUTHOR]

This study investigated the effect of Cs-137 absorbed from the upper part (surface of leaves and husk) of rice plants and its accumulation in grains. Cesium-137 content in brown rice plants sprayed with Cs-137 onto the plant surface before ear emergence, flowering, and grain filling stages were 5, 109, and 76 Bq/kg, respectively. Comparison of Cs-137 concentration between mature and immature grains revealed that brown rice can easily absorb Cs-137 at the beginning of grain filling. [ABSTRACT FROM AUTHOR]

Purpose: Stage shift is considered a major reason for more favorable outcomes in patients with screen-detected breast cancer. However, even after adjusting for clinical stage, unresolved issues concerning the reasons for a survival benefit associated with screening programs remain. This study aims to evaluate differences in subtype distribution and outcomes among patients with screen-detected and symptomatic invasive breast cancer and assess whether variations in subtype distribution could explain differences in prognosis. Methods: Survival analysis was performed to estimate the likelihood of distant recurrence and death in 1132 patients. Subtypes were defined as luminal A [estrogen receptor (ER)+ and/or progesterone receptor (PR)+, human epidermal growth factor receptor 2 (HER2)−, and Ki67 low], luminal B (HER2−) (ER+ and/or PR+, HER2−, and Ki67 high), luminal B (HER2+) (ER+ and/or PR+ and HER2+), HER2 overexpressing (ER−, PR−, and HER2+), and triple negative (ER−, PR−, and HER2−). Results: Screen-detected cancers had favorable clinicopathological characteristics, such as smaller tumor size and a lower frequency of lymph node involvement. Women with screen-detected cancers had a survival advantage. Subtype distribution differed significantly among women with screen-detected and symptomatic cancer. Screen-detected cancers were more likely to be luminal A and less likely to be HER2 overexpressing or triple negative cancer compared with symptomatic cancers (luminal A 61.3 vs. 44.2%, HER2 overexpressing 4.0 vs. 8.0%, triple negative 8.0 vs. 15.9%). Node status, mode of detection, and subtype were independent prognostic factors in the multivariate analysis. Conclusions: Differences in subtype distribution between screen-detected and symptomatic cancer could partially explain differences in outcomes. [ABSTRACT FROM AUTHOR]

Allogeneic stem cell transplantation (allo-SCT) is a curative option for patients with relapsed follicular lymphoma (FL). Prospective studies of reduced-intensity conditioning (RIC) have revealed that chemosensitivity at allo-SCT is the most reliable predictor of outcome; however, limited data are available for progressive/refractory disease. We report here a retrospective analysis of RIC allo-SCT for patients with FL. The purpose of this study was to elucidate the role of allo-SCT for patients with relapsed/refractory FL. We analyzed 46 patients-11 (24%) transplanted in CR, 6 (13%) transplanted in PR and 29 (63%) with progressive/refractory disease. The estimated 5-year overall survival rate was 71.6% (95% confidence interval (CI), 51.5-84.5%). According to the disease status at transplantation, the 5-year survival rate was 80.7% (95% CI, 37.7-95.4%) in the patients with CR or PR and 66.1% (95% CI, 41.5-82.3%) in those with progressive/refractory disease (P=0.29). There were no differences in relapse/progression and non-relapse mortality between the patients with chemosensitive disease and progressive/refractory disease. Allo-SCT may be a valuable treatment option, even for patients with progressive/refractory FL. [ABSTRACT FROM AUTHOR]

Chronic granulomatous disease (CGD), a primary immunodeficiency caused by impaired phagocyte killing of intracellular pathogens, is characterized by recurrent, life-threatening, bacterial and fungal infections. As a result of improvements in microbiologic culture and identification techniques, a number of unique filamentous fungi have been reported as significant pathogens in patients with CGD. We report a case of subcutaneous basidiomycete Phellinus mori infection in a patient with CGD. To the best of our knowledge, this is the first reported case of human infection by this fungus. The causative fungus was identified on the basis of its morphological characteristics and nucleotide sequence on the internal transcribed spacer region of the ribosomal RNA gene. This is the fifth case report of filamentous basidiomycetes infecting a patient with CGD; all of these cases have been caused by Phellinus species. We highlight the importance of recognizing filamentous basidiomycetes Phellinus species as possible agents of non- Aspergillus fungal infections in patients with CGD. [ABSTRACT FROM AUTHOR]

The one-neutron removal cross section from Ne, Mg, and Si on a Pb target have been measured at around 240 MeV/nucleon at the RIBF (RI-Beam Factory) at RIKEN. This main goal of the experiment was to extract the inclusive Coulomb breakup cross sections in order to probe possible halo structures through their enhanced low-energy E1 strengths - so-called soft E1 excitations. The results showed significant enhancement of the one-neutron removal Coulomb breakup cross section for Mg. This in turn provides evidence for the existence of halo structure in Mg. [ABSTRACT FROM AUTHOR]

The efficacy of unrelated transplantation for patients with ALL who lack an HLA-matched sibling remains unclear. We performed a decision analysis to determine the efficacy of myeloablative transplantation from a genetically HLA-A, -B, -DRB1 allele-matched unrelated donor for patients with Ph chromosome-negative ALL aged 21-54 years. The transition probabilities were estimated from the Japan Adult Leukemia Study Group studies (ALL93; n=80, ALL97; n=82), and the Japan Marrow Donor Program database (transplantation in first CR (CR1): n=177). The primary outcome measure was the 10-year survival probability with or without quality of life (QOL) adjustment. Subgroup analyses were performed according to risk stratification based on the WBC count and cytogenetics, and according to age stratification. In all patients, unrelated transplantation in CR1 was shown to be superior in analyses both with and without QOL adjustment (40.8 vs 28.4% and 43.9 vs 29.0%, respectively). A similar tendency was observed in all subgroups. The decision model was sensitive to the probability of leukemia-free survival following chemotherapy and the probability of survival after transplantation in standard-risk and higher-aged patients. Unrelated transplantation in CR1 improves the long-term survival probability in patients who lack an HLA-matched sibling. However, recent improvements in treatment strategies may change this result. [ABSTRACT FROM AUTHOR]

We present how radioactive Cs was deposited on wheat, rice, peach tree and soil after nuclear accident in Fukushima. The deposition of radioactive Cs was found as spots at the surface of the leaves, branch or trunk of the trees, as well as in soil using one of the imaging method, autoradiography. The deposited radioactive Cs was not easily washed out, even with the treatment of acid solution. When the wheat was harvested 2 months after the accident, high radioactivity of Cs was found only on the leaves developed and expanded at the time of the accident. In the case of the rice grain, most of the radioactivity was found in bran and the radioactivity was drastically reduced in milled rice. Most of the radioactive Cs accumulation in rice plants was estimated from the absorption of the Cs ion dissolved in water, rather than Cs adsorbed in soil. [ABSTRACT FROM AUTHOR]

The magnesium (Mg) uptake of rice seedlings treated with different pH solutions was investigated using tracer of Mg. The application of Mg enabled the determination of Mg uptake amount within 15 min of the different pH treatments. The uptake amount of Mg and its distribution in rice plants were determined by using the imaging plate. The result demonstrated that the Mg uptake amount was approximately 3 μmol/15 min per plant in 0.27 mM Mg solution at pH 5.6, and this uptake decreased by 30 % at lower pH conditions (pH 4.5). On the contrary, the pH 6.5 condition induced the Mg uptake activity to 4.4 μmol/15 min. The inverse relationship found between proton concentration in nutrient solution and the Mg uptake amount within 15 min of treatment demonstrated that the low pH condition could inhibit the Mg uptake activity as a consequence of the competition between Mg and proton. It is concluded that under low pH conditions, regardless of other factors, there is a decrease of Mg uptake activity in rice roots. [ABSTRACT FROM AUTHOR]

The neutron-rich oxygen isotope O has been investigated via proton inelastic scattering on a liquid hydrogen target at 63.5 MeV/nucleon. The invariant mass method in inverse kinematics was employed to reconstruct the energy spectrum. A sharp resonance state was observed at 42(5) keV above the neutron threshold. A comparison of the differential cross section leading to this state with a microscopic DWBA calculation allowed a J assignment of 5/2 for this state. [ABSTRACT FROM AUTHOR]

The unbound excited states of the most neutron-rich dripline oxygen isotope, O, have been investigated by using the O( p, p′)O reaction at the beam energy of 62 MeV/nucleon in inverse kinematics. The first and second unbound excited states of O have been observed at $${E_{\rm x}= 4.63_{-0.14}^{+0.30}}$$ MeV and $${E_{\rm x}= 5.13_{-0.24}^{+0.19}}$$ MeV (preliminary) along with the evidence for another higher lying state at around 7.3 MeV. The quadrupole deformation parameter $${\beta_{2^+}}$$ was deduced to be $${0.15_{-0.03}^{+0.08}}$$ (preliminary) for the first time. The systematics of the $${\beta_{2^+}}$$ and the $${E_{\rm x}(2_1^+)}$$ in the Z = 8 isotopes shows the N = 16 spherical shell closure in O. [ABSTRACT FROM AUTHOR]

Allogeneic hematopoietic SCT (allo-HCT) from matched sibling donor (MSD) is recommended for younger patients with intermediate cytogenetic risk AML in first CR (CR1), whereas the role of alternative donor transplants in these patients is unknown. We retrospectively analyzed 605 patients with intermediate-risk AML, who received myeloablative allo-HCT in CR1. The 4-year OS for MSD (n=290) and matched unrelated donor (MUD; n=141) was 65% and 68% (P=0.50), respectively. In multivariate analysis, MUD had a similar risk of overall mortality as MSD (hazard ratio=0.90; 95% confidence interval, 0.62-1.30; P=0.58), whereas older age, female donor/male recipient (FDMR) combination, and requiring more than one course of induction chemotherapy to achieve CR1 were poor prognostic factors for OS. Thus, OS after MUD HCT with sex combinations other than FDMR was significantly higher than that after MSD HCT from female donors to male recipients (4-year OS 72% versus 55%, P=0.04). These results suggest that HCT, not only from MSD, but also from MUD, should be considered in younger patients with intermediate-risk AML in CR1, and that the donor-recipient sex combination is more important than the donor type in donor selection. [ABSTRACT FROM AUTHOR]

We present a case in which an amyloid lung nodule was diagnosed preoperatively by amyloid scintigraphy (99m)Tc-aprotinin. A 65-year-old man complained of marked weight loss (9 kg) over a period of 6 months. An abnormal shadow in the middle field of the right lung was detected on chest X-ray, corresponding to a 16-mm nodule in the right middle lobe on thoracic computed tomography (CT). Total protein and immunoglobulin G levels were elevated to 8.3 and 2245 mg/dl, respectively, but other blood tests including several tumor marker levels and Cryptococcus antibodies were all within normal range. Fluorodeoxyglucose positron emission tomography showed no uptake by the lung nodule, so lung amyloidosis was considered as differential diagnosis. To avoid risk of bleeding on bronchoscopy, noninvasive amyloid scintigraphy using (99m)Tc-aprotinin was first performed. A nodular, abnormal accumulation was observed in the right middle lung lobe. Diagnostic imaging strongly suggested amyloidosis, so video-assisted thoracic surgery was performed rather than bronchoscopy. Pathological samples showed positive staining with Congo red, and A-λ amyloidosis was diagnosed on the basis of immunostaining. Scintigraphy using (99m)Tc-aprotinin offers a useful, noninvasive method for assessing lung amyloidosis. [ABSTRACT FROM AUTHOR]

To study the effects of M-CSF administration on long-term outcomes of unrelated BMT, we retrospectively analyzed data from patients transplanted through the Japan Marrow Donor Program. We obtained data from 54 patients who received M-CSF just after BMT and 500 patients who did not receive M-CSF or G-CSF acted as controls. There were no significant differences between the two cohorts with respect to OS, acute GVHD or relapse. Although the incidence of chronic GVHD was comparable between the two groups, extensive chronic GVHD was observed significantly less often in the M-CSF cohort than in the control group. Multivariate analysis identified M-CSF as a significant factor for attenuating extensive chronic GVHD (relative risk: 0.73; 95% confidence interval: 0.55-0.94; P=0.012). We also found the same results in matched-pair analysis. Our observation suggests the potential for clinical use of M-CSF to dampen severe chronic GVHD. [ABSTRACT FROM AUTHOR]

The dependence on atmospheric stability of flow characteristics adjacent to a very rough surface was investigated in a larch forest in Japan. Micrometeorological measurements of three-dimensional wind velocity and air temperature were taken at two heights above the forest, namely 1.7 and 1.2 times the mean canopy height h. Under near-neutral and stable conditions, the observed turbulence statistics suggest that the flow was likely to be that of the atmospheric surface layer (ASL) at 1.7 h, and of the roughness sublayer (RSL) at 1.2 h. However, in turbulence spectra, canopy-induced large coherent motions appeared clearly at both heights. Even under strongly stable conditions, the large-scale motions were retained at 1.2 h, whereas they were overwhelmed by small-scale motions at 1.7 h. This phenomenon was probably due to the enhanced contribution of the ASL turbulence associated with nocturnal decay of the RSL depth, because the small-scale motions appeared at frequencies close to the peak frequencies of well-known ASL spectra. This result supports the relatively recent concept that canopy flow is a superimposition of coherent motions and the ASL turbulence. The large-scale motions were retained in temperature spectra over a wider region of stability compared to streamwise wind spectra, suggesting that a canopy effect extended higher up for temperature than wind. The streamwise spacing of dominant eddies according to the plane mixing-layer analogy was only valid in a narrow range at near neutral, and it was stabilised at nearly half its value under stable conditions. [ABSTRACT FROM AUTHOR]

Purpose: We have performed percutaneous vertebroplasty (PV) using polymethylmethacrylate (PMMA) for patients with vertebral metastases since 2002. This study investigated the therapeutic effects of PV on vertebral metastases.Materials and Methods: A retrospective (2002-2008) review was conducted for 69 consecutive patients with 141 metastatic vertebrae treated with PV using PMMA. The clinical background of the patients, visual analog scale (VAS), improvement rate, outcomes, and complications were evaluated.Results: The mean preoperative VAS score was 7.3 and significantly improved to 1.9 postoperatively (at discharge), with a mean improvement rate of 73.3%. With regard to complications, no new fractures of adjacent vertebral bodies were encountered, but cement leakage was seen in 49% of the patients. Most patients were asymptomatic during the postoperative course, although two patients (3%) experienced dyspnea that was suspected to be adult respiratory distress syndrome or a pulmonary embolism.Conclusion: PV can offer pain relief to patients with painful vertebral metastases and short life expectancy whose general condition makes surgery difficult. [ABSTRACT FROM AUTHOR]

A letter to the editor is presented in response to the article related to the generation of novel human cell-based models for the loss of human neural progenitor cells (NPC) in disrupted-in-schizophrenia 1 (DISC1) neurodevelopment and neuropsychiatric disorder.

Human group A rotavirus (GAR) G12 strains are regarded as potentially important pathogens for acute gastroenteritis. On the other hand, to date, the only report of detection of G12 in animals was that of a porcine G12P[7] strain RU172. Strain RU172 formed a separate G12 lineage, distinct from human G12 strains, and by analyses of deduced amino acid sequences, had a VP4, VP6, NSP4-5 of porcine origin. In the present study, we determined the full-length nucleotide sequences of VP1, VP3, and NSP1-3 genes and nearly full-length nucleotide sequence of VP2 gene of RU172. By nucleotide sequence identities and phylogenetic analyses, the VP7-VP4-VP6-VP1-VP2-VP3-NSP1-NSP2-NSP3-NSP4-NSP5 genes of RU172 were assigned to G12-P[7]-I5-R1-C1-M1-A1-N1-T1-E1-H1 genotypes, respectively. Within their respective genotypes, (i) VP1 gene of RU172 exhibited higher genetic relatedness to Wa-like human G12 GARs than porcine strains, (ii) VP2-3 and NSP2 genes clustered separately from the Wa-like human (including G12) and porcine clusters, while (iii) the VP6, NSP1 and NSP3-5 genes clustered with porcine and porcine-like human strains. These observations suggested that (i) the porcine G12 strain might have originated from porcine–human reassortment events, or alternatively, (ii) the Wa-like human and porcine G12 strains might have originated from a common ancestor, and eventually evolved (by genetic drift and shift) with time. Our findings provided important insights into the possible patterns of evolution of the porcine G12 strain. [ABSTRACT FROM AUTHOR]

BACKGROUND AND AIMS: The early detection and prevention of dementia is attracting attention. We therefore developed an easily performed protocol to identify patients with memory impairment which may progress to dementia, and evaluated its validity. METHODS: We focused on short-term memory impairment alone, and named the test, consisting of 3 tasks, the simplified Short-Term Memory recall Test (STMT; with a maximum score of 8). Patients were classified into a memory impairment group of 26 subjects and a control group of 23 subjects. At the first examination, subjects underwent the STMT, MMSE and ADAS-Jcog. as cognitive function tests. Follow-up observations were performed for 2 years at 6-month intervals. RESULTS: There were significant differences in the mean scores for all tests, except for MMSE memory items between the 2 groups. When the cut-off value of STMT was established as 4 points, and scores lower than this value were defined as memory impairment, the sensitivity and specificity were highest, 73.1% and 82.6%, respectively. Sensitivity and specificity also rose to 92.3% and 95.7%, respectively, when STMT scores were added together with those of ADAS-Jcog. Results of logistic regression analysis indicated that development into Alzheimer's disease 2 years later was significantly correlated with STMT scores at first examination. The incidence of progression to Alzheimer's disease in patients with scores /=5. CONCLUSIONS: This study suggests the usefulness of the STMT for identifying memory impairment as a pre-dementia state. [ABSTRACT FROM AUTHOR]

Studies on bovine group B rotaviruses (GBRs) are limited. To date, only the VP6 gene of a single bovine GBR strain and the VP7 and NSP5 genes of a few bovine GBR strains have been sequenced and analyzed. In the present study, using a single-primer amplification method, we have determined the full-length nucleotide sequences of the VP1, VP2, VP4, VP6, NSP1 and NSP2 genes of three bovine GBR strains from eastern India. In all six of these genes, the bovine GBR strains shared high genetic relatedness among themselves but exhibited high genetic diversity with cognate genes of human, murine and ovine GBRs. Interestingly, as with group A rotaviruses, the bovine GBR VP1, VP2, VP6 and NSP2 genes appeared to be more conserved than the VP4 and NSP1 genes among strains of different species. The present study provides important insights into the genetic makeup and diversity of bovine GBRs, and also identifies a novel GBR VP4 genotype. [ABSTRACT FROM AUTHOR]

Purpose: To determine the prevalence and progression of Barrett's epithelium and associated risk factors in Japan.Methods: The study population comprised 869 cases. Endoscopic Barrett's epithelium was diagnosed based on the Prague C & M Criteria. The correlations of clinical factors with the prevalence and progression of endoscopic Barrett's epithelium were examined.Results: Endoscopic Barrett's epithelium was diagnosed in 374 cases (43%), in the majority of which the diagnosis was short-segment Barrett's esophagus. The progression of Barrett's epithelium was identified in 47 cases. In univariate and multiple logistic regression analyses, aging, smoking habit, and erosive esophagitis were significantly associated with the prevalence of Barrett's epithelium, whereas aging and erosive esophagitis, especially severe erosive esophagitis, were significant contributing factors to the progression of Barrett's epithelium.Conclusions: Forty-three percent of the total study population was diagnosed as having endoscopic Barrett's epithelium. During the follow-up period, 12.6% of the cases with Barrett's epithelium exhibited progression which was associated with aging and severe erosive esophagitis. [ABSTRACT FROM AUTHOR]

Epidemiological studies suggest a positive association between nutrient intake, hyperinsulinemia and risk of Benign prostatic hyperplasis (BPH). This study tests the hypothesis that a low-fat, high-fiber diet and daily exercise would lower serum insulin and reduce the growth of serum-stimulated primary prostate epithelial cells in culture. Serum samples were obtained from eight overweight men before and after the Pritikin residential, 2-week diet and exercise intervention and from seven men who were long-term followers of the low-fat, high-fiber diet and regular exercise lifestyle. The serum was used to stimulate primary prostate epithelial cells in culture. Growth was measured after 48 and 96 h and apoptosis after 96 h. At 48 h there was no significant difference in growth within the Pre, 2-week or Long-Term groups. At 96 h growth was significantly reduced in the 2-week (13%) and in the Long-Term (14%) groups compared to the Pre data. At 96 h, apoptosis was not significantly different among the three groups. Fasting insulin was reduced by 30% in the 2-week group and by 52% in the Long-Term group compared to the Pre data. Testosterone was unchanged in the 2-week group. The results of this study indicate that a low-fat, high-fiber diet and daily exercise lowers insulin and reduces growth of prostate primary epithelial cells and suggests that lifestyle may be an important factor in the development or progression of BPH. Future prospective trials should address the effects of this lifestyle modification on BPH symptomatology and progression.Prostate Cancer and Prostatic Diseases (2008) 11, 362–366; doi:10.1038/pcan.2008.6; published online 19 February 2008 [ABSTRACT FROM AUTHOR]

We carried out instrumental neutron activation analysis (INAA) as well as k0-based prompt gamma-ray analysis ( k0-PGA) to measure the amount of the elements in onions and studied whether the onions collected from different sites can be categorized based on the elemental concentration profile. Six elements (Na, Mg, Cl, K, Ca, Mn) and 3 elements (B, S, Cl) were measured by INAA and PGA in the onions grown in two districts, Hokkaido and Saga, in Japan, respectively. After principal component analysis, it was found that Cl was an important element to feature the producing districts of onions. [ABSTRACT FROM AUTHOR]

During 2004–2006, group A rotavirus P[8] strains were the major VP4 genotype (43.2%, n = 317) among diarrhoeic children in Eastern India. Phylogenetic analysis of VP8* amino acid sequences of 16 of these strains with other P[8] strains revealed four distinct lineages. P[8] strains from Eastern India clustered within rare OP354-like and Hun9-like lineages, pointing towards co-prevalence of divergent P[8] strains. Although it is unclear whether the observed genetic diversity might affect to some extent the efficacy of vaccines, the present study emphasized further efforts to address the much lacking information on diversity of P[8] strains across the Indian subcontinent. [ABSTRACT FROM AUTHOR]

We demonstrate a high-speed polarization-insensitive photoconductor based on intersecting InP nanowires synthesized between a pair of hydrogenated silicon electrodes deposited on amorphous SiO2 surfaces prepared on silicon substrates. A 14-ps full width at half maximum de-embedded impulse response is measured, which is the fastest reported response for a photodetector fabricated using nanowires. The high-speed electrical signal measurements from the photoconductor are performed by an integrated coplanar waveguide transmission line. The demonstrated ability to grow intersecting InP nanowires on hydrogenated microcrystalline Si surfaces will facilitate the construction of ultra-fast photodetectors on a wide range of substrates. [ABSTRACT FROM AUTHOR]

Three rotavirus variants with a rearranged RNA segment derived from the NSP3 gene were isolated in three independent experiments of coinfection and multiple passages of simian rotavirus strain SA11 and single-VP7-gene- or NSP1-gene-substitution reassortants having genetic background of SA11. Sequence analysis indicated that the three rearranged NSP3 genes had almost identical sequences and genomic structures organized by partial duplication of the open reading frame in a head-to-tail orientation following the termination codon. The junction site of the original NSP3 gene (first copy) and the duplicated portion (second copy) was identical among the three rearranged genes, while a direct repeat, i.e., a homologous sequence between the first copy and second template for duplication, typically located at the junction site, was not detected. However, short similar sequences were present at the end of the first copy and beginning of the second copy. These findings suggest that rearrangement of the NSP3 gene may occur at a certain preferential site which is related to sequence similarity between 3′-untranslated region and a region near the 5′-end of ORF. [ABSTRACT FROM AUTHOR]

The determination of the host ranges of viruses is important because of the possible emergence of infectious agents, which may result from the zoonotic transmission of animal viruses to humans. The family Nodaviridae, whose members are non-enveloped, positive-stranded bipartite RNA viruses, is comprised of the genera Alphanodavirus and Betanodavirus, whose members predominantly infect insects and fish, respectively. The alphanodaviruses can also infect suckling mice and suckling hamsters, resulting in paralysis and death. Pigs near the site of isolation of the Nodamura virus (NoV), an alphanodavirus, have been reported to have high levels of NoV neutralizing antibody, suggesting that they may be part of the natural host range of this virus. Betanodaviruses are the causative agents of viral nervous necrosis, which occurs in several species of fish. However, little is known regarding the mechanism of infection of these viruses. Whether betanodaviruses can infect hosts other than fish remains unclear. In this study, we examined the possibility that a betanodavirus, redspotted grouper nervous necrosis virus (RGNNV), can infect human cell lines and showed that this virus can attach to the cells but cannot penetrate them, although human cells can support the replication of the betanodavirus when viral RNAs are transfected. The betanodavirus in its present form cannot infect human cells. [ABSTRACT FROM AUTHOR]

Betanodaviruses, members of the family Nodaviridae, have small positive-stranded bipartite RNA genomes and are the causal agent of viral nervous necrosis (VNN) in many species of marine farmed fish. In the aquaculture industry, outbreaks of betanodavirus infection and spread in larval and juvenile fish result in devastating damage and heavy economic loss. Although an urgent need exists to develop drugs that inhibit betanodavirus infection, there have been no reports about anti-betanodavirus drugs. Recently, it was reported that betanodaviruses were detected in the endosomes of infected cells, suggesting that betanodaviruses enter fish cells by endocytosis. This finding prompted us to examine whether blocking this endosomal pathway could provide a target for antiviral drug development. In this study, we examined the inhibitory effect of several lysosomotropic agents against betanodavirus infection in fish E-11 cells. The presence of 1 mM NH4Cl or 1 µM chloroquine in the medium inhibited the entry of betanodaviruses into cells and inhibited viral infection. The lysosomotropic agents bafilomycin A1 and monensin also inhibited virus-induced cytopathology and virus production. Our data demonstrate that inhibitors of endosomal acidification are candidates as antiviral agents against betanodavirus. [ABSTRACT FROM AUTHOR]

During a surveillance study, four of 130 group A rotavirus strains, detected from diarrheic calves in Eastern India, exhibited G3P[3] specificities. Molecular characterization of VP7 and VP8* genes of one such strain [named as RUBV3 (RU: ruminant and BV: bovine)] revealed genetic relatedness to a G3P[3] simian strain, RRV, and RRV-related caprine strain GRV. Strain RUBV3 had VP6, NSP4 and NSP5 genes of bovine origin. Therefore, the present study provides evidence for multiple reassortment events involving ruminant and simian strains and, to our knowledge, is the first report of detection of bovine group A rotavirus strains with G3P[3] specificities. [ABSTRACT FROM AUTHOR]

To compare epidemiologic features and genetic characteristics of group A rotaviruses causing diarrhea in children and adults, a survey was conducted in Wuhan, China, during the period of Dec. 2000–May 2006. A total of 3839 stool specimens from diarrheal patients from eight hospitals were analyzed. Winter seasonality was observed for rotavirus diarrhea in both adults and children, showing overall rotavirus-positive rates of 9.0 and 23.9%, respectively. Throughout the study period, G3 was the most frequent G serotype in both adults and children (detection rates 86.2 and 87.8%, respectively), and was mostly associated with VP4 genotype P[8], VP 6 genotype II (subgroup II), and NSP4 genotype B. G3 rotaviruses were differentiated into eight electropherotypes, among which seven types were found in specimens from both adults and children. VP7 gene sequences of G3 rotaviruses from adults and children (6 and 4 strains, respectively), detected in different years and different hospitals, showed extremely high sequence identities (99–100%) to each other and to a few G3 rotavirus strains reported in Asia. However, lower sequence identities (82–96%) were observed to most of the human and animal G3 rotaviruses reported so far, including some Chinese strains. These findings indicate that in Wuhan, China, epidemic and genetic features of rotaviruses are similar in adults and children, and it has been suggested that G3 rotaviruses that might have originated from the same rotavirus were circulating among children and adults as prevailing viruses. In this study, two rotavirus strains, G9P[8] strain L169, derived from an adult, and G4P[6] strain R479, derived from a child, were isolated and genetically analyzed. The VP7 gene of L169 belongs to a major lineage of G9 rotaviruses that are globally widespread, but is distinct from G9 rotaviruses reported previously in China. The strain R479 had a VP7 gene which was divergent from most G4 human rotaviruses and showed an unusual dual subgroup specificity, I + II. The R479 VP6 gene does not belong to the main clusters of subgroup I and II rotaviruses phylogenetically, but is related to those of the porcine rotaviruses and some unusual human rotaviruses represented by the RMC321 strain isolated in eastern India. [ABSTRACT FROM AUTHOR]

Although application of the Edmonton protocol has markedly improved the outcome for pancreatic islet transplantation, the insulin independence rate after islet transplantation from one donor pancreas has remained low. During the isolation process and subsequent clinical transplantation, islets are subjected to severe adverse conditions that impair survival and ultimately contribute to graft failure. The aim of this study was to map the c-Jun NH2-terminal kinase (JNK) pathway that mediates islet loss during islet transplantation and to clarify whether intraportal injection with JNK inhibitor during islet transplantation can prevent islet graft loss. We measured JNK activity in the liver, fat and muscle of diabetic mice and in the liver immediately after islet transplantation. We examined the effect of intraportal injection of JNK inhibitory peptide at islet transplantation. JNK activity became progressively higher at least until 24 h after transplantation. The cell-permeable peptide of JNK inhibitor was delivered not only in the liver but also in other insulin target organs, preventing JNK activation in the liver at least until 24 h after transplantation and reducing JNK activity in these insulin target organs. Moreover, the peptide inhibitor prevented islet graft loss immediately after transplantation and improved islet transplant outcome. These findings suggest that control of the JNK pathway is extremely important in islet transplantation and that intraportal injection of JNK inhibitor during islet transplantation (addition of JNK inhibitor to transplant media) could prevent the impairment of islet cells, leading to improved outcome for pancreatic islet transplantation. [ABSTRACT FROM AUTHOR]

Elemental concentration in each tissue of Japanese morning-glory ( Pharbitis nil) during the growth was analyzed by INAA. Plants were grown in water culture for 4 days under 12-hour light and 12-hour dark condition. During the growth, 10 plants in the same developmental stage were periodically harvested. Then the plants were separated into 9 tissues to determine elemental concentration and water content. There was an elemental specific profile within the plant tissue, for example, Na and K concentrations were high in root whereas Mg and Ca concentrations were high at the upper part of the plants. Among the elements studied, Ca and Mg showed rhythmical change in concentration, increased during the day and decreased during the night. This tendency was especially noted at shoot apex. However, water content in all of the tissue was increased about 6 hours prior to the light period and showed maximum in the middle of the light period. About 6 hour difference of water content movement to those of Ca and Mg concentration suggested that water movement drives more dynamic change of each elemental concentration within a plant. [ABSTRACT FROM AUTHOR]

Molecular characterization of two porcine group A rotavirus strains (HP113 and HP140), detected from eastern India, revealed a VP7 closely related to those of human G6P[14] strains, VP4 with a borderline P[13] genotype, and VP6 related to bovine and human SGI strains rather than porcine SGI and/or SGII group A rotaviruses. Both strains had NSP4 and NSP5 of porcine origin. Therefore, to our knowledge, the present study is the first report of detection of group A rotavirus strains with G6P[13] genotype specificities and provides evidence for independent segregation of the VP6- and NSP4-encoding genes in porcine group A rotaviruses. [ABSTRACT FROM AUTHOR]