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This is the last of three papers describing the use of electron microscopy and antigenic tests intended to characterize and place in taxa more than 60 previously unclassified viruses. The first paper describes the viruses we classified as members of the families Arenaviridae, Paramyxoviridae, or Poxviridae. The second paper describes those classified as members of the virus family Bunyaviridae. Another paper, published separately, discusses viruses classified as members of the family Rhabdoviridae. In this paper we report that electron microscopy provided sufficient evidence to provisionally place 14 viruses in the family Reoviridae. By using serologic methods, we placed Minnal virus in the Umatilla serogroup and established new antigenic groups for seven other viruses (Ieri, Picola, Arkonam, Tembe, Fomede, Wongorr, and Gomoka). No antigenic relationships were determined for six other viruses (Andasibe, Itupiranga, Kammavanpettai, Lake Clarendon, Matucare, and Ndelle) provisionally placed in the family Reoviridae. [ABSTRACT FROM AUTHOR]

During approximately 35 years, investigators in various laboratories studying arbovirus ecology and epidemiology accumulated many virus isolates, more than 60 of which were not characterized or placed in taxa. By a combination of electron microscopic and antigenic studies we collected information sufficient to provisionally classify 60 isolates. Electron microscopic observations suggest that 20 are members of the virus family Bunyaviridae, 20 Rhabdoviridae, 14 Reoviridae, one Togaviridae, one Paramyxoviridae (Mapuera virus, from a bat), and one Poxviridae (Yoka virus, from mosquitoes). Serologic studies provided evidence sufficient to place some of these viruses in recognized antigenic groups, within families and genera, and to establish new antigenic groups and taxa for others. Three viruses were found to have morphologic and morphogenetic characteristics consistent with those of members of the family Arenaviridae: Quaranfil virus, a human pathogen, Johnston Atoll virus, isolated from birds and ticks, and Araguari virus, isolated from an opossum. This, the first in a series of three papers, described methods used for these investigations and also presents descriptions of viruses provisionally placed in the families Arenaviridae, Paramyxoviridae, or Poxviridae. Descriptions of viruses provisionally placed in families Bunyaviridae and Reoviridae are published in the second and third papers, respectively. Viruses of the family Rhabdoviridae have been described separately. [ABSTRACT FROM AUTHOR]

This is the second of three papers describing the use of electron microscopy and antigenic analyses intended to characterize and place in taxa more than 60 previously unclassified viruses. The first paper of the series describes the viruses we classified as provisional members of the families Arenaviridae, Paramyxoviridae, or Poxviridae; another paper, published separately, discusses the Rhabdoviridae. In this paper we report that electron microscopy provided sufficient evidence to place 17 of these viruses (Belem, Erve, Estero Real, Mojui dos Campos, Nyando, Odrenisrou, Okola, Pacora, Para, Santarem, Tanga, Telok Forest, Termeil, Thiafora, Thottapalayam, Wanowrie, and Yacaaba) in the family Bunyaviridae and to support the observations of others that Yogue and Kasokero viruses are members of this virus family. Subsequent antigenic studies allowed us to place some of these viruses in recognized antigenic groups and to establish new antigenic groups for others. [ABSTRACT FROM AUTHOR]

Preparations of eastern encephalitis virus were disrupted by treatment with sodium deoxycholate. Tween 80 and ether, and sodium lauryl sulfate. The effect of these treatments on the viral specific activities of eastern encephalitis virus were studied. Untreated and disrupted preparations of eastern encephalitis virus were physically characterized by buoyant density centrifugation in cesium chloride density gradients and by rate zonal sedimentation in sucrose density gradients. Disruption by both sodium deoxycholate and Tween 80-ether resulted in the release of hemagglutinating subunits of higher density than the hemagglutinin associated with the intact virion. The hemagglutinating subunits released by both treatments were similar in density and size. A dense complement-fixing component, believed to represent released viral nucleoprotein core, was detected in cesium chloride gradients after sodium deoxycholate treatment of the virus. It was not detected in untreated preparations or in preparations treated with Tween 80-ether. Even at low concentrations, sodium lauryl sulfate was destructive of all viral activities save complement-fixation activity. The hemolytic activity of eastern encephalitis virus was physically closely associated with infectivity and hemagglutinating activity of the virus and was apparently dependent on the structural integrity of the virion. [ABSTRACT FROM AUTHOR]

Reports on the isolation of a paramyxovirus of mice, isolated in North Queensland. Isolation of the virus on four separate occasions from moribund wild mice by kidney autoculture; Comparison of the virus to parainfluenza III virus; Lack of evidence for a virus-associated or soluble hemagglutinin, or hemadsorbing properties, using standard techniques with erythrocytes from mouse, rat, guinea-pig, rabbit, chicken, goose, pig, sheep, cattle, horse or human Type O.