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Venous thrombosis is a frequent disorder. A distinction is made between an acute phase of the disease and a chronic manifestation, the postthrombotic syndrome. In particular, proximal venous thrombosis/pelvic vein thrombosis can cause a life-threatening pulmonary embolism during the acute phase of the disease. The postthrombotic syndrome is characterized by the remodeling of the affected venous section, which is often caused by inflammation. Locally, the typical clinical finding is caused by scarred stricture of the vein with restricted drainage and peripheral venous hypertension. Acute thrombosis should be primarily treated by therapeutic anticoagulation and compression therapy of the affected extremity. The duration of these measures depends on clinical presentation, cause (provoked, unprovoked) and risk factors for venous thrombosis/recurrent thrombosis. Venous revascularization procedures are important both in the acute phase of the disease and in the treatment of postthrombotic syndrome. The recanalization treatment is mostly carried out as an endovascular or hybrid intervention and venous bypass procedures are reserved for special situations. [ABSTRACT FROM AUTHOR]

Background: The German Society for Vascular Surgery and Vascular Medicine (Deutsche Gesellschaft für Gefäßchirurgie und Gefäßmedizin, DGG) represents over 3100 members. In addition to continuing education and professional policy positioning, an important area is the promotion of research and science as well as their visibility. The DGG has set up an online-based platform on its official homepage with the primary goal of providing site-specific information on research content, equipment, and contact information of university centers for vascular surgery. With the introduction of this digital research map, the DGG has created a project that is innovative in the German-speaking environment. Initiation and implementation: In 2022, the DGG Board of Directors established the prerequisite with the necessary funding decision and commissioned the DGG Research Commission to implement the content. The commission determined which parameters should be shown on the map and surveyed them by means of standardized questionnaires at the research institutions in vascular surgery in Germany. The commission prepared the feedback for the digital presentation, web editors and web developers programmed the website including search functions, and the DGG head office entered the data. At the outset, 18 locations were mapped. The map is intended to grow to enable more research centers to submit their data. Discussion: The digital research map is the result of a dedicated collaboration between, among others, the research commission, the management, the service providers, and the board of the DGG. The aim was to create a user-friendly platform where information on the various research groups can be accessed quickly and easily. A research map is an important digitally accessible statement that underscores the self-concept of the specialty of vascular surgery with its claim to innovation and academic basis. Visibility of research activity is a relevant element of highly specialized vascular medicine. The need for digital representation of research structures in academic medicine is confirmed by comparable efforts of other scientific institutions such as the German Society for Surgery (Deutsche Gesellschaft für Chirurgie, DGCH) (1) and the German Rectors' Conference (Hochschul- Rektorenkonferenz, HRK) (2) The research map of the DGG differs from the above-mentioned projects by highlighting the translational aspect. Scientific research groups are not presented as such, but rather the overall constellation of the clinical or academic university institution. Conclusion: The DGG digital research map represents a pioneering initiative to strengthen collaboration and information exchange in vascular research by providing a comprehensive overview of working groups, research topics, and equipment. The outlook for the future is promising, as the digital research map will be continuously expanded and updated to meet the needs of researchers. Likewise, it provides visibility of the research accomplishments of the vascular surgery community to students, researchers from other disciplines, journalists, industry, and interested parties from abroad. [ABSTRACT FROM AUTHOR]

We investigated whether T cell-recruiting bispecific anti-CD3/GD2 antibody NG-CU might be an alternative to therapeutic anti-GD2 monoclonal antibody (mAb) ch14.18, mediating complement-dependent cytotoxicity (CDC) and antibody-dependent cell-mediated cytotoxicity (ADCC) through natural killer (NK) cells for immunotherapy in high-risk/relapsed neuroblastoma after autologous/allogeneic stem cell transplantation (auto/alloSCT). Different antibody concentrations and effector-to-target ratios (E:T) were evaluated using xCELLigence RTCA system, peripheral blood mononuclear cells (PBMCs) (healthy donors and patients after alloSCT), and neuroblastoma cell lines (LS/LAN-1). Mean specific lysis of LS cells utilizing PBMCs from healthy donors and ch14.18 (1 µg/ml) was 40/66/75% after 12/24/48 h compared to 66/93/100% in the presence of NG-CU (100 ng/ml). NG-CU showed enhanced cytotoxicity compared to ch14.18, even at lower concentrations and E:T ratios, and completely eradicated LS cells after 72 h. To decipher the influence of effector cell subsets on lysis, different ratios of T and NK cells were tested. At a ratio of 1:1, ch14.18 was more effective than NG-CU. Using patient PBMCs taken at different time points posttransplant, significant lysis with both constructs was detectable depending on percentages and total numbers of T and NK cells; in the early posttransplant phase, NK cells were predominant and ch14.18 was superior, whereas later on, T cells represented the majority of immune cells and NG-CU was more effective. Our study highlights the importance of analyzing effector cell subsets in patients before initiating antibody-based therapy. Consequently, we propose an adjusted administration of both antibody constructs, considering the state of posttransplant immune recovery, to optimize anti-tumor activity. [ABSTRACT FROM AUTHOR]

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The aging of Y1Ba2Cu3O7-δ thin film structures after low energy 30 keV electron irradiation has been investigated. The irradiation was aimed at creating artificial channels with suppressed superconductivity that would enforce the coherent flow of vortices. Through transport measurements, the irradiated regions were found to degrade gradually with the time of aging contrary to non-irradiated parts with good time stability of superconducting properties. Raman spectroscopy and atomic force microscopy investigations have revealed the presence of a relatively thick carbon contamination layer on the top of YBCO films originating from electron irradiation. X-ray photoelectron spectroscopy analysis supported by the evolution of Raman spectra suggests that carbon penetrating YBCO structures may be responsible for chemical interaction with the YBCO species to form C-O bonds, following the time evolution of the Raman spectra. [ABSTRACT FROM AUTHOR]

The transition temperature, TC, of two types of optimally doped cuprate high-TC superconductor films, YBa2Cu3O7-δ and La1.85Sr0.15CuO4, is found to increase upon linking Au nanoparticles to their surface via organic molecules. At the same time, and quite surprisingly, the critical current is reduced. We attribute these results to screening of the Coulomb interactions and smearing the pinning potential landscape. The transition temperature was increased also when the Au NPs were not chemically linked to the surface, consistent with the screening scenario. The linking molecules, however, enable selective adsorption of the gold nanoparticles and their separation from the surface, thus eliminating the conventional proximity effect. [ABSTRACT FROM AUTHOR]

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Copyright of Gefaesschirurgie is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Gefaesschirurgie is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This study was aimed to identify characteristics of ED patients who discontinued PDE5i despite successful intercourse. Data were collected using a questionnaire from 34 urologic clinics regardless of the effect (success or failure) of PDE5i treatment by visiting the clinics (717), e-mail (64) or post (101) for 882 ED patients who had previously taken any kind of PDE5i on demand four or more times. Discontinuation of PDE5i was defined if the patient had never taken PDE5i for the previous 1 year despite successful intercourse. Of the 882 patients, 485 were included in the final analysis. Difference in the socio-demographic, ED- and partner-related data between the continuation and discontinuation group and factors influencing discontinuation of the PDE5i were analyzed. Among 485 respondents (mean age, 53.6), 116 (23.9%) had discontinued PDE5i use despite successful intercourse. Most common reasons for the discontinuation were 'reluctant medication-dependent intercourse' (31.0%), 'spontaneous recovery of erectile function without further treatment' (30.2%), and 'high cost' (26.7%). In multiple logistic regression analysis, independent factors influencing discontinuation of the drug were cause of ED (psychogenic), short duration of ED, low education (⩽ middle school), and religion (Catholic). In partner-related compliance, only partner's religion (Catholic) was a significant factor. [ABSTRACT FROM AUTHOR]

Magnetic properties of compacted SmCaMnO nanoparticles with average particle size ranging from 20 to 80 nm have been investigated in wide temperature and magnetic field range. It has been found that charge ordering transition gradually shifts to lower temperatures with decreasing particle size and almost disappears for 20 nm particles. At the same time, the relative volume of the ferromagnetic phase increases monotonously. Field-induced transition from antiferromagnetic to ferromagnetic state in 80 nm particles appears at the same magnetic field as in the bulk. In small 20 nm particles, the transition is strongly suppressed by increasing surface spins disorder. Magnetic hysteresis loops show size-dependent exchange bias effect with exchange field, remanence asymmetry, and magnetic coercivity that depend on cooling magnetic field and temperature. Magnetic training effect has been observed in 20 nm particles and analyzed using a spin relaxation model. The thermoremanence and isothermoremanence curves have provided fingerprints of irreversible magnetization originating from the glassy component. Analysis of remanence curves has showed that the inner core of small 20 nm particles behaves as a two-dimensional diluted antiferromagnet. [ABSTRACT FROM AUTHOR]

Purpose: The evaluation of latent tuberculosis infection (LTBI) is recommended before kidney transplantation. The interferon-γ release assay has been reported to be more specific than the tuberculin skin test (TST) for detecting LTBI. We compared the TST and QuantiFERON-TB Gold In-Tube test (QFT-GIT) for the screening for LTBI and determined the agreement between the two tests in renal transplant recipients before transplantation. Methods: Adult patients who were evaluated for renal transplantation between May 2010 and February 2012 at Severance Hospital in South Korea were prospectively enrolled. We performed TST and QFT-GIT. Results: Of the 126 patients, 23 (19.3 %) had positive TST results and 53 (42.1 %) had positive QFT-GIT results. Agreement between the TST and QFT-GIT was fair (κ = 0.26, P < 0.001). The induration size of TST was significantly correlated with a positive rate of QFT-GIT ( P = 0.015). Age (odds ratio [OR] 1.08, 95 % confidence interval [CI] 1.03-1.13, P = 0.003), male sex (OR 2.73, 95 % CI 1.17-6.38, P = 0.021), and risk for LTBI (OR 4.62, 95 % CI 1.15-18.64, P = 0.031) were significantly associated with positive QFT-GIT results. For positive TST results, only male sex was associated (OR 4.29, 95 % CI 1.40-13.20, P = 0.011). Conclusion: The positivity for QFT-GIT was higher than the positivity for TST, and QFT-GIT more accurately reflected the risk for LTBI. However, a further longitudinal study is needed in order to confirm that the QFT-GIT test can truly predict the development of TB after renal transplantation. [ABSTRACT FROM AUTHOR]

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Copyright of Gefaesschirurgie is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Magnetic properties of electron-doped LaCaMnO manganite nanoparticles, with average size of 12 and 60 nm, prepared by the glycine-nitrate method, have been investigated in the temperature range 5-300 K and magnetic fields up to 90 kOe. It is suggested that weak ferromagnetic moment results from ferromagnetic shells of the basically antiferromagnetic nanoparticles and from domains of frustrated disordered phase in the core. Assumption of two distinct sources of ferromagnetism is supported by the appearance of two independent ferromagnetic contributions in the fit of the T Bloch law to spontaneous magnetization. The ferromagnetic components, which are more pronounced in smaller particles, occupy only a small fraction of the nanoparticle volume and the antiferromagnetic ground state remains stable. It is found that the magnetic hysteresis loops following field cooled processes, display size-dependent horizontal and vertical shifts, namely, exhibiting exchange bias effect. Time-dependent magnetization dynamics demonstrating two relaxation rates were observed at constant magnetic fields upon cooling to T < 100 K. [ABSTRACT FROM AUTHOR]

Although premixed charge compression ignition (PCCI) combustion engines are praised for potentially high efficiency and clean exhaust, experimental engines built to date emit more hydrocarbons (HCs) and carbon monoxide (CO) than the conventional machines. These compounds are not only strictly controlled components of the exhaust gas of road vehicles but are also an energy loss indicator. The prime objective of this study was to investigate the major sources of the HCs formed in the combustion chamber of an experimental PCCI engine in order to suggest some effective technologies for HC reduction. In this study, to explore the dominant sources of HC emissions in both operation modes, a single cylinder engine was prepared such that it could operate using either conventional diesel combustion or PCCI combustion. Specifically, the contributions of the top-ring crevice volume in the combustion chamber and the bulk quenching of the lean mixture were investigated. To understand the influence of the shape and magnitude of the crevice on HC emissions, the engine was operated with 12 specially prepared pistons with different top-ring crevices installed one after another. The engine emitted proportionally more HCs as the depth of the crevice increased as long as the width remained narrower than the prevailing quench distance. The top-ring-crevice-originated exhaust HCs comprised approximately 31% of the total HC emissions in the baseline condition. In a series of tests to estimate the effects of bulk quench on exhaust HC emissions, intake air was heated from 300K to 400K in steps of 25K. With the intake air heated, HC and CO emissions decreased with a gradually diminishing rate to zero at 375K. In conclusion, the most dominant sources of HC emissions in PCCI engines were the crevice volumes in the combustion chamber and the bulk quenching of the lean mixtures. The key methods for reducing HC emissions in PCCI engines are minimizing crevice volume in the combustion chamber and maximizing intake air temperature allowed based on the permissible NOx level. [ABSTRACT FROM AUTHOR]

The therapeutic efficacy of humanized or chimeric second-generation antitumor antibodies is clearly established, but often limited. In recent years, defined modifications of the glycosylation pattern or the amino-acid sequence of the human immunoglobulin G1 Fc part have resulted in the development of third-generation antibodies with improved capability to recruit Fc receptor-bearing effector cells. The first antibodies of this kind, currently evaluated in early clinical trials, are directed against lymphoma-associated antigens. Fc-engineered antibodies targeting myeloid leukemia are not yet available. We here report on the generation and preclinical characterization of an Fc-optimized antibody directed to the FMS-related tyrosine kinase 3 (FLT3), an antigen expressed on the leukemic blasts of all investigated patients with acute myeloid leukemia (AML). This antibody, termed 4G8SDIEM, mediated markedly enhanced cellular cytotoxicity against FLT3-expressing cell lines as well as blasts of AML patients. FLT3 expression levels on AML cells varied between 300 and 4600 molecules/cell and, in most cases, were substantially higher than those detected on normal hematopoietic precursor cells and dendritic cells (approximately 300 molecules/cell). Antibody-mediated cytotoxicity against these normal cells was not detectable. 4G8SDIEM has been produced in pharmaceutical quality in a university-owned production unit and is currently used for the treatment of leukemia patients. [ABSTRACT FROM AUTHOR]

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Magnetic and transport properties of LaCaMnO nanoparticles with average size of 18 nm and Curie temperature T≈231 K have been studied. Pronounced irreversibility of magnetization below T≈208 K has been observed. Studied particles have shown memory effects in zero-field-cooled and field-cooled magnetization. The resistivity has a semiconducting character at 150< T<300 K and exhibits relaxation and memory effects below the Curie temperature. The results suggest that superspin-glass features in ensembles of interacting 18 nm LaCaMnO particles appear along with superferromagnetic-like features. [ABSTRACT FROM AUTHOR]

The article discusses a study that examined the possible application of the zirconium-titanium-nickel-copper-beyllium (Zr-Ti-Ni-Cu-Be) amorphous alloy as a filler for the low temperature brazing between dissimilar Ti and stainless steels (STS). Two major problems in Ti-STS are described. Changes made to the microstructure of the Ti-STS dissimilar joints brazed with the Zr-Ti-Ni-Cu-Be amorphous filler for the increased brazing temperature are also explained.

Compression ignition of homogeneous charges in internal combustion (IC) engines is expected to offer high efficiency of DI diesel engines without high levels of NOx and particulate emissions. This study is intended to find ways of extending the rich limit of HCCI operation, one of the problems yet to be overcome. Exhaust emissions characteristics are also explored through analyses of the combustion products. DME fuel, either mixed with air before induction or directly injected into the combustion chamber of a rapid compression and expansion machine, is compressed to ignite under various conditions of compression ratio, equivalence ratio, and injection timing. The characteristics of the resulting combustion and exhaust emissions are discussed in terms of the rate of heat release computed from the measured pressure, and the concentrations of THC, CO, and NOx are measured by FT-IR and CLD. The experimental data to date show that operation without knock is possible with mixtures of higher equivalence ratio when DME is directly injected rather than when it is inducted in the form of a perfectly homogeneous fuel-air mixture. Although fuel injected early in the compression stroke promotes homogeneity of the DME-air mixture in the cylinder, it causes the mixture to ignite too early to secure good thermal efficiency and knock-free operation at high loads. Low temperature reactions occur at about 660K regardless of the fueling methods, fuel injection timing and equivalence ratio. The main components of hydrocarbon emissions turned out to be unburned fuel (DME), formaldehyde and methane. [ABSTRACT FROM AUTHOR]

We have previously reported that r28M, a recombinant bispecific single-chain antibody directed to a melanoma-associated proteoglycan (NG2) and the costimulatory CD28 molecule on T cells, induced T-cell activation, which resulted in tumor-cell killing. T-cell activation did not require a primary signal through the T-cell antigen receptor (TCR)/CD3 complex and depended on the presence of NG2-positive tumor cells. Here, we further investigate this phenomenon of a target cell-restricted, supra-agonistic CD28 stimulation with bispecific antibodies. To this end, we exchanged the NG2 targeting part of r28M with a single-chain antibody directed to the B-cell associated antigen CD20. The resulting bispecific single-chain antibody, termed r2820, induced supra-agonistic T-cell activation, which required the presence of autologous normal or malignant B cells, respectively. Once activated, T cells were capable of destroying lymphoma target cells.These findings demonstrate that supra-agonistic CD28 stimulation with bispecific single-chain antibodies is a robust and readily reproducible phenomenon. In the context of experimental tumor therapy, it may provide a valuable alternative to the unrestricted T-cell activation induced by ‘super-agonistic’, monospecific CD28 antibodies.Leukemia (2009) 23, 71–77; doi:10.1038/leu.2008.271; published online 2 October 2008 [ABSTRACT FROM AUTHOR]

The purpose of this phase III clinical trial was to compare two different extracellular contrast agents, 1.0 M gadobutrol and 0.5 M gadopentate dimeglumine, for magnetic resonance imaging (MRI) in patients with known or suspected focal renal lesions. Using a multicenter, single-blind, interindividual, randomized study design, both contrast agents were compared in a total of 471 patients regarding their diagnostic accuracy, sensitivity, and specificity to correctly classify focal lesions of the kidney. To test for noninferiority the diagnostic accuracy rates for both contrast agents were compared with CT results based on a blinded reading. The average diagnostic accuracy across the three blinded readers ('average reader') was 83.7% for gadobutrol and 87.3% for gadopentate dimeglumine. The increase in accuracy from precontrast to combined precontrast and postcontrast MRI was 8.0% for gadobutrol and 6.9% for gadopentate dimeglumine. Sensitivity of the average reader was 85.2% for gadobutrol and 88.7% for gadopentate dimeglumine. Specificity of the average reader was 82.1% for gadobutrol and 86.1% for gadopentate dimeglumine. In conclusion, this study documents evidence for the noninferiority of a single i.v. bolus injection of 1.0 M gadobutrol compared with 0.5 M gadopentate dimeglumine in the diagnostic assessment of renal lesions with CE-MRI. [ABSTRACT FROM AUTHOR]

Copyright of Der Radiologe is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This paper proposes a steering control method based on optimal control theory to improve the maneuverability of a six-wheeled vehicle during cornering. The six-wheeled vehicle is believed to have better performance than a four-wheeled vehicle in terms of its capability for crossing obstacles, off-road maneuvering and fail-safe handling when one or two of the tires are punctured. Although many methods to improve the four-wheeled vehicle’s lateral stability have been studied and developed, there have only been a few studies on the six-wheeled vehicle’s lateral stability. Some studies of the six-wheeled vehicle have been reported recently, but they are related to the desired yaw rate of a four-wheeled vehicle to control the six-wheeled vehicle’s maneuvering during corning. In this paper, the sideslip angle and yaw rate are controlled to improve the maneuverability during cornering by independent control of the steering angles of the six wheels. The desired yaw rate that is suitable for a six-wheeled vehicle is proposed as a control target. In addition, a scaled-down vehicle with six drive motors and six steering motors that can be controlled independently is designed. The performance of the proposed control methods is verified using a full model vehicle simulation and scaled-down vehicle experiment. [ABSTRACT FROM AUTHOR]

Dollar spot caused by Sclerotinia homoeocarpa F. T. Bennett is the most economically important turf disease on golf courses in North America. Dollar spot resistance in a creeping bentgrass cultivar would greatly reduce the frequency, costs, and environmental impacts of fungicide application. Little work has been done to understand the genetics of resistance to dollar spot in creeping bentgrass. Therefore, QTL analysis was used to determine the location, number and effects of genomic regions associated with dollar spot resistance in the field. To meet this objective, field inoculations using a single isolate were performed over 2 years and multiple locations using progeny of a full sib mapping population ‘549 × 372’. Dollar spot resistance seems to be inherited quantitatively and broad sense heritability for resistance was estimated to be 0.88. We have detected one QTL with large effect on linkage group 7.1 with LOD values ranging from 3.4 to 8.6 and explaining 14–36% of the phenotypic variance. Several smaller effect QTL specific to rating dates, locations and years were also detected. The association of the tightly linked markers with the LG 7.1 QTL based on 106 progeny was further examined by single marker analysis on all 697 progeny. The high significance of the QTL on LG 7.1 at a sample size of 697 ( P < 0.0001), along with its consistency across locations, years and ratings dates, indicated that it was stable over environments. Markers tightly linked to the QTL can be utilized for marker-assisted selection in future bentgrass breeding programs. [ABSTRACT FROM AUTHOR]

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Transport properties of self-doped La0.89MnO3 single crystals with Néel temperature of TN ≈139 K have been investigated in wide temperature range 10–300 K. Data suggests that current at low temperature is conducted through a strongly temperature-dependent, but almost bias independent channel operating in parallel with a bias controlled but temperature independent channel. The first channel is associated with transport across an insulating antiferromagnetic matrix while the latter one represents tunnel conductivity through intrinsic tunnel junctions appearing due to interruption of conducting percolating paths by phase separated insulating inclusions. Tunnel character of the conductivity manifests itself in nonlinear current-voltage characteristics and appearance of a zero-bias anomaly in the form of a prominent conductance peak in the vicinity of zero bias. Zero bias anomaly and V-shaped characteristics of the differential conductance at high voltages are ascribed to the formation of local magnetic states in the insulating region of the tunneling junction. [ABSTRACT FROM AUTHOR]

Rituximab, a chimeric mouse/human monoclonal antibody that binds to the CD20 antigen, is part of current treatment of many B-cell malignancies and several autoimmune diseases. Very few cases of rituximab administration during pregnancy have been described. We report here the case of rituximab administration during the first trimester of pregnancy in a woman with autoimmune hemolytic anemia. No significant effects were observed in B-cell counts or the immune status of the newborn.Journal of Perinatology (2006) 26, 252–255. doi:10.1038/sj.jp.7211481 [ABSTRACT FROM AUTHOR]

We summarize our key developments in nanoimprint lithography (NIL) that employs a single layer resist lift-off process: lowering of the imprint temperature (for thermal imprint) and pressure, achieving uniform resist thickness and low residual resist layer thickness in the trenches, and eliminating metal ‘rabbit ears’ for the single-layer lift-off. In thermal NIL, our requirements for lower operating temperature and pressure motivated us to develop an alternative resist that is a viscous fluid at room temperature and cures at a lower temperature of 70 °C than the operating temperature of the conventional thermal NIL (≈200 °C). For UV NIL, we devised a method to dispense the resist onto a hydrophobic mold and use the hydrophilic substrate surface to spread the resist via surface wetting to engineer a continuous and uniform film. We also explored the use of Si(110) substrates as molds to produce features with perfectly vertical side walls, and the use of aqua regia to directly etch away rabbit ears. [ABSTRACT FROM AUTHOR]

Transport properties of phase separated La0.8Ca0.2MnO3 crystals in the aged highly resistive metastable state were studied. It was found that the coexistence of different ferromagnetic phases at low temperatures is sensitive to electric current/field. In a contrast with the previously studied low resistivity metastable states the high resistivity state exhibits positive magnetoresistance and significant current dependence of the resistivity even at temperatures much higher than the Curie temperature. Application of current pulses results in appearance of zero bias anomaly in the current dependent conductivity. Similarly to the low resistivity metastable states the memory of the resistivity can be erased only after heating of the sample to Te ≈360 K. After one year storage at room temperature the La0.8Ca0.2MnO3 samples show clear signatures of aging. The aged samples spontaneously evolute towards high resistivity states. The results are discussed in the context of a coexistence of two ferromagnetic phases with different orbital order and different conductivity. The metallic ferromagnetic phase seems to be less stable giving rise to the experimentally observed electric field effects and aging. [ABSTRACT FROM AUTHOR]

Gray leaf spot (GLS) is a serious fungal disease caused by Magnaporthe grisea, recently reported on perennial ryegrass ( Lolium perenne L.), an important turfgrass and forage species. This fungus also causes rice blast and many other grass diseases. Rice blast is usually controlled by host resistance, but durability of resistance is a problem. Little GLS resistance has been reported in perennial ryegrass. However, greenhouse inoculations in our lab using one ryegrass isolate and one rice-infecting lab strain suggest presence of partial resistance. A high density linkage map of a three generation Italian × perennial ryegrass mapping population was used to identify quantitative trait loci (QTL) for GLS resistance. Potential QTL of varying effect were detected on four linkage groups, and resistance to the ryegrass isolate and the lab strain appeared to be controlled by different QTL. Of three potential QTL detected using the ryegrass isolate, the one with strongest effect for resistance was located on linkage group 3 of the MFB parent, explaining between 20% and 37% of the phenotypic variance depending on experiment. Another QTL was detected on linkage group 6 of the MFA parent, explaining between 5% and 10% of the phenotypic variance. The two QTL with strongest effect for resistance to the lab strain were located on linkage groups MFA 2 and MFB 4, each explaining about 10% of the phenotypic variance. Further, the QTL on linkage groups 3 and 4 appear syntenic to blast resistance loci in rice. This work will likely benefit users and growers of perennial ryegrass, by setting the stage for improvement of GLS resistance in perennial ryegrass through marker-assisted selection. [ABSTRACT FROM AUTHOR]

Creeping bentgrass ( Agrostis stolonifera L.) is one of the most adapted bentgrass species for use on golf course fairways and putting greens because of its high tolerance to low mowing height. It is a highly outcrossing allotetraploid species (2 n=4 x=28, A2 and A3 subgenomes). The first linkage map in this species is reported herein, and it was constructed based on a population derived from a cross between two heterozygous clones using 169 RAPD, 180 AFLP, and 39 heterologous cereal and 36 homologous bentgrass cDNA RFLP markers. The linkage map consists of 424 mapped loci covering 1,110 cM in 14 linkage groups, of which seven pairs of homoeologous chromosomes were identified based on duplicated loci. The numbering of all seven linkage groups in the bentgrass map was assigned according to common markers mapped on syntenous chromosomes of ryegrass and wheat. The number of markers linked in coupling and repulsion phase was in a 1:1 ratio, indicating disomic inheritance. This supports a strict allotetraploid inheritance in creeping bentgrass, as suggested by previous work based on chromosomal pairing and isozymes. This linkage map will assist in the tagging and eventually in marker-assisted breeding of economically important quantitative traits like disease resistance to dollar spot ( Sclerotinia homoeocarpa F.T. Bennett) and brown patch ( Rhizoctonia solani Kuhn). [ABSTRACT FROM AUTHOR]

The efficiency of test vaccines needs to be evaluated by quantification of the triggered cellular immune response. Usually, for these assays, autologous target cells expressing the vaccine antigen are required. In the context of messenger RNA (mRNA)-based vaccinations, the target cells used for the read-out are mRNA-transfected monocyte-derived dendritic cells (Mo-DCs). Their production typically requires samples of 100 ml blood from the patients, and limits the number of assays that can be performed. We show here that fresh peripheral blood mononuclear cells (PBMCs) can be transfected with mRNA by electroporation. Such cells are as efficient as mRNA-transfected Mo-DCs for their ability to activate memory T cells in vitro. Thus, mRNA-transfected PBMCs are a convenient replacement of mRNA-transfected Mo-DCs for the in vitro monitoring of natural or vaccine-induced immune responses. [ABSTRACT FROM AUTHOR]

Summary:Relapse is a major problem after allogeneic transplantation in children with acute B-lineage lymphoblastic leukemias (ALL) and lymphomas and additional therapeutic strategies are needed to increase graft versus leukemia effects without inducing graft versus host disease (GvHD). Several studies have shown the efficacy of a humanized CD20 antibody (rituximab) for treatment of CD20+ malignancies together with conventional chemotherapy but less is known about its post transplant usefulness. We studied the ability of rituximab to mediate antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) with effector cells and complement from patients who were transplanted with T-cell-depleted grafts from unrelated or mismatched related donors. Highest lytic activity (ADCC) was observed against leukemia-derived MHH4 cells and Burkitt's lymphoma-derived Raji cells in the first months after transplantation, corresponding to the high percentage of regenerating CD56+CD16+ cells. Moreover, primary cryopreserved ALL-blasts from a pediatric patient were also efficiently lysed. Increased lysis was obtained after stimulation with interleukin-2. Combination of ADCC and CDC had additive effects. These findings encourage clinical trials on the use of rituximab for improving minimal residual disease control and relapse prevention after allogeneic high-risk transplantation in the small group of pediatric patients with CD20+ leukemias/lymphomas.Bone Marrow Transplantation (2005) 36, 91–97. doi:10.1038/sj.bmt.1705014 Published online 23 May 2005 [ABSTRACT FROM AUTHOR]

An restriction fragment length polymorphism (RFLP)-based genetic map of ryegrass (Lolium) was constructed for comparative mapping with other Poaceae species using heterologous anchor probes. The genetic map contained 120 RFLP markers from cDNA clones of barley (Hordeum vulgareL.), oat (Avena sativaL.), and rice (Oryza sativaL.), covering 664 cM on seven linkage groups (LGs). The genome comparisons of ryegrass relative to the Triticeae, oat, and rice extended the syntenic relationships among the species. Seven ryegrass linkage groups were represented by 10 syntenic segments of Triticeae chromosomes, 12 syntenic segments of oat chromosomes, or 16 syntenic segments of rice chromosomes, suggesting that the ryegrass genome has a high degree of genome conservation relative to the Triticeae, oat, and rice. Furthermore, we found ten large-scale chromosomal rearrangements that characterize the ryegrass genome. In detail, a chromosomal rearrangement was observed on ryegrass LG4 relative to the Triticeae, four rearrangements on ryegrass LGs2, 4, 5, and 6 relative to oat, and five rearrangements on ryegrass LGs1, 2, 4, 5, and 7 relative to rice. Of these, seven chromosomal rearrangements are reported for the first time in this study. The extended comparative relationships reported in this study facilitate the transfer of genetic knowledge from well-studied major cereal crops to ryegrass. [ABSTRACT FROM AUTHOR]

We have developed a process to fabricate a cross-bar structure using UV-curable nanoimprint lithography with a UV-curable double-layer spin-on resist, metal lift off and Langmuir-Blodgett film deposition. This process allowed us to produce 1-kbit cross-bar memory circuits at 30-nm half-pitch on both top and bottom electrodes. Read, write, erase and cross talking were also investigated. [ABSTRACT FROM AUTHOR]

In this study, Zr-Ti-Cu-Ni-Be bulk metallic glass (BMG) alloys containing a small amount of crystalline phase particles were fabricated by strip casting, and their improvement of mechanical properties and fracture toughness was explained by direct observation of the microfracture process. The compressive and fracture toughness test results indicated that strength, strain to failure, and fracture toughness of the strip-cast BMG alloy containing coarse crystalline particles were higher than those of the as-cast monolithic BMG alloy or the strip-cast BMG alloy containing fine crystalline particles. From in-situ microfracture observations, the improvement of overall mechanical properties of the strip-cast BMG alloy containing coarse crystalline particles could be interpreted by taking consideration of both the existence of coarse crystalline particles and the role of the particles to block crack propagation and to form multiple shear bands. Such property improvement suggests new applicability of the strip-cast BMG alloys containing coarse crystalline particles, which can work as toughening and strengthening reinforcements, to structures and components requiring excellent mechanical properties. [ABSTRACT FROM AUTHOR]

In the context of developing a safe genetic vaccination strategy we tested and studied globin-stabilized mRNA-based vaccination in mice. This vaccination strategy has the advantages of genetic vaccination (easy production, adaptability to any disease and inexpensive storage when lyophilized), but not the drawbacks of DNA vaccination (long-term uncontrolled expression of a transgene, possibility of integration into the host genome and possible induction of anti-DNA antibodies). We report here that injection of naked ß-globin untranslated region (UTR)-stabilized mRNA coding for ß-galactosidase is followed by detectable translation in vivo. In addition, we show that such a vaccination strategy primes a T helper 2 (Th2) type of response which can be enhanced and shifted to a Th1-type immune response by application of recombinant granulocyte/macrophage colony-stimulating factor 1 day after mRNA injection. Our data demonstrate that the administration of globin UTR-stabilized mRNA is a versatile vaccination strategy that can be manipulated to fit the requirement of antiviral, antibacterial or antitumor immunity. [ABSTRACT FROM AUTHOR]

The resistive and magnetic properties of La1-xCaxMnO3 single crystals are investigated. In particular, properties of close-to-critically doped crystals with x = 0.22 are confronted with those of underdoped crystals with x = 0.18 and x = 0.2 and optimally doped crystals with x = 0.3. A systematic investigation of nonlinear transport shows that the critically doped crystal does not simply constitute an intermediate case between strongly nonlinear underdoped and linear, optimally doped one. The observed low-temperature resistivity increase can be interpreted in terms of orbital ordering or as a manifestation of an intrinsic tunnelling mechanism. The transport measurements have been completed by magneto-optic investigations which suggest that the low temperature resistivity of the underdoped x = 0.18 compound is dominated by tunnelling through intrinsic barriers associated with twin domains, while phase separation dominates in the critically doped x = 0.22 compound. [ABSTRACT FROM AUTHOR]

: The prion protein (PrPC) is essential for susceptibility to transmissible spongiform encephalopathies. A specific conformer of this protein (PrPSc) is, according to the 'protein only' hypothesis, the principal or only component of the infectious agent, designated prion. Transmission of prions between species is often inefficient, resulting in low attack rates and/or prolonged incubation times and is ascribed to a 'species barrier' caused by differences in the amino acid sequence of PrP between recipient and donor. In this report, we demonstrate that these differences in amino acid sequence result in presentation of distinct peptides on major histocompatibility complex class II molecules. These peptides result in activation of specific CD4+ T cells which leads to the induction of an effective immune response against foreign PrP as demonstrated by antibody production. Therefore, CD4+ T cells represent a crucial component of the immune system to distinguish between foreign and self PrP. [ABSTRACT FROM AUTHOR]

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Copyright of Der Urologe A is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Samaria-doped ceria powders were prepared by the sol-gel method with different processes. The characteristics of the sample were investigated by particle size distribution, X-ray diffraction, crystallite size, and density analyses. A modified process of the sol-gel method was proposed in this work. It involves treating the gel with high-carbon (long chain, high boiling point) alcohol. It yields near-completely soft-agglomerated nanocrystalline powders which are easily sintered in air to yield near-fully relative density at 1300°C, which is significantly lower than temperatures of 1400–1500°C required by the doped-ceria powder prepared via the previous processes of the sol-gel method. [ABSTRACT FROM AUTHOR]