1. Aberrant expression of Notch signaling molecules in patients with immune thrombocytopenic purpura.
- Author
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Daoxin Ma, Jianjian Dai, Xiaojuan Zhu, Shuxin Yan, Ping Zhao, Jingru Zhang, Yuanyuan Zhu, Jianzhi Sun, Jun Peng, Chunyan Ji, Ming Hou, Ma, Daoxin, Dai, Jianjian, Zhu, Xiaojuan, Yan, Shuxin, Zhao, Ping, Zhang, Jingru, Zhu, Yuanyuan, Sun, Jianzhi, and Peng, Jun
- Subjects
GENE expression ,THROMBOCYTOPENIA ,REVERSE transcriptase ,POLYMERASE chain reaction ,IMMUNOCYTOCHEMISTRY ,THERAPEUTICS ,PROTEIN metabolism ,CALCIUM-binding proteins ,CELL receptors ,CELLULAR signal transduction ,COMPARATIVE studies ,GROWTH factors ,IMMUNOHISTOCHEMISTRY ,RESEARCH methodology ,MEDICAL cooperation ,MEMBRANE proteins ,PROTEINS ,RESEARCH ,THROMBOPENIC purpura ,WESTERN immunoblotting ,EVALUATION research ,REVERSE transcriptase polymerase chain reaction - Abstract
To investigate the role of Notch signaling pathway in immune thrombocytopenic purpura (ITP), we measured the expression of 11 Notch pathway molecules in ITP patients and evaluated their clinical relevance. Real-time reverse transcriptase polymerase chain reaction results showed there was aberrant expression of some Notch molecules in ITP. Notch1 and Notch3 expression elevated, while Notch2 decreased statistically in ITP patients. As for Notch ligands, only DLL1 was found downregulated in ITP. The expression of Notch target gene, Hes1, was also upregulated. In accordance with the mRNA level, Notch1 and Hes1 protein expression was also found elevated by Western blot. Immunocytochemistry showed that Notch1 expressed highly in the cytomembrane, cytoplasm, and part of cellular nucleus for ITP while weak in cytomembrane for controls, and Hes1 of ITP was found expressed higher in cellular nucleus than that of controls. Our findings suggest that the aberrant expression profile of Notch pathway may be involved in ITP, and blockage of Notch1 pathway is likely a promising therapeutic concept. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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