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The Lower Cretaceous Choshi Group in the Choshi Geopark, a member of the Japanese Geoparks Network, includes conglomerate layers with chert gravels. We extracted Cisuralian (early Permian) and Early–Middle Triassic radiolarians and Middle Triassic conodonts from chert pebbles within the Choshi Group; previous studies reported Guadalupian (middle Permian) and Middle Jurassic radiolarians. Although the previous highlights of the Choshi Geopark were the Cretaceous–Quaternary strata, the microfossils from the gravels could extend the age range of fossils from the geopark. The extension using of microfossils from the gravels can be applied to other geoparks that contain coarse clastic sediments and/or rocks. [ABSTRACT FROM AUTHOR]

Purpose: In a previous study, protein tyrosine phosphatase non-receptor type (PTPN) 3 was identified as an immune checkpoint molecule in lymphocytes, and its potential as a novel target for cancer immunotherapy was anticipated. However, evaluation of dendritic cell (DC) function as antigen-presenting cells is critical for the development of immunotherapy. In this study, we aimed to analyze the biological effect of PTPN3 on DCs induced from human peripheral blood monocytes obtained from healthy individuals. Methods: We used short-interfering RNA to knock down PTP3 in DCs. For DC maturation, we added cancer cell lysate and tumor necrosis factor-α/interferon-α to immature DCs. In the cytotoxic assay, the target cancer cells were SBC5, unmatched with DCs from healthy human leukocyte antigen (HLA)-A24, or Sq-1, matched with DCs. Enzyme-linked immunosorbent assay was used to determine the amount of cytokines. To examine the intracellular signaling system, intracellular staining was used. Results: PTPN3 knockdown significantly increased the number of DCs, expression of CD80 and chemokine receptor (CCR)7, and production of interleukin-12p40/p70 in mature DCs. In the HLA-A24-restricted DC and human lung squamous cell carcinoma cell cytotoxic assay, inhibition of PTPN3 expression in mature DCs induced cytotoxic T lymphocytes with increased production of INF-γ and granzyme B, and enhanced toxicity against cancer cells and migration to cancer. Furthermore, inhibition of PTPN3 expression activated the mitogen-activated protein kinase pathway in DCs. Conclusion: Based on our findings, inhibition of PTPN3 expression could contribute to the development of novel cancer immunotherapies that activate not only lymphocytes but also DCs. [ABSTRACT FROM AUTHOR]

Objectives: Advances in thoracoscopic surgery have made skin incisions smaller, but there are concerns about cancer cell contamination during sample extraction. We performed retrieval bag lavage cytology (BLC) during thoracoscopic surgery to evaluate the risk of cancer dissemination and the prognostic influence of BLC status. Methods: BLC was investigated in 893 patients who underwent thoracoscopic lobectomy or segmentectomy for lung cancer between 2013 and 2018. The clinicopathological features and prognosis were compared between the BLC-positive and BLC-negative groups. Results: Forty-nine patients (5.5%) were positive for BLC. BLC correlated with pleural invasion (49.0% vs. 12.9%, P < 0.001); however, BLC was positive in 3.3% of cases without pleural invasion. Multivariate analysis revealed that tumor size, lymph node metastasis, lymphatic and pleural invasion were predictive factors for positive BLC. Prognosis was poorer in the BLC-positive group than in the BLC-negative group (5-year overall survival, 73.6% vs. 90.2%, P < 0.001); nevertheless, positive BLC was not an independent prognostic factor. The locoregional recurrence rate was higher among BLC-positive patients than among BLC-negative patients, whereas there was no significant difference in the distant recurrence rate. Positive BLC was associated with locoregional recurrence (hazard ratio 1.87, P = 0.044) and the correlation was stronger in stage I lung cancer. There were no cases of extraction bag breakage or port-site recurrence. Conclusions: BLC positivity was correlated with the risk of locoregional recurrence in patients with surgically resected lung cancer, although it was not an independent prognostic factor. Careful manipulation is essential for extracting specimens from the thoracic cavity. [ABSTRACT FROM AUTHOR]

Background: Liposarcoma arising from the mediastinum is rare, accounting for less than 1% of mediastinal tumors. Furthermore, a rapidly growing well-differentiated liposarcoma is extremely rare. A well-differentiated liposarcoma is usually considered a low-grade malignancy. However, we present an extremely rare case of a sclerosing variant of well-differentiated liposarcoma that grew rapidly within a year. Case presentation: A 77-year-old man with a giant mass in the left thoracic cavity was referred to our hospital. This mass measured about 10 cm and occupied the left-sided mediastinum on a chest radiography; however, there was no abnormal finding on the previous year's chest radiography. Chest-enhanced computed tomography revealed a well-circumscribed 11-cm mass in the left-sided anterior mediastinum. Positron emission tomography showed accumulation of fluorodeoxyglucose uptake in this tumor (maximum standard uptake value = 3.3). The radiological findings of computed tomography and positron emission tomography indicated that this tumor was a benign or low-grade malignancy; therefore, the chest radiographic findings were difficult to explain. To explain this discrepancy and establish the diagnosis, tumor resection was performed via left posterolateral thoracotomy. Intraoperatively, the left phrenic nerve and pericardium were adhered tightly to the tumor, so we resected them. The tumor was well-circumscribed and fibrous; therefore, the initial diagnosis was solitary fibrous tumor. However, based on its histopathological and immunohistochemical patterns, the tumor was diagnosed as a sclerosing variant of well-differentiated liposarcoma. Five years postoperatively, the patient remains alive with no evidence of disease recurrence. Conclusions: A well-differentiated liposarcoma is usually considered a low-grade malignancy. Nevertheless, the giant tumor in the present case appeared within 1 year. Thus, this was an extremely rare case of a sclerosing variant of well-differentiated liposarcoma with rapid growth. [ABSTRACT FROM AUTHOR]

The total carbon content analysis for Takashima-oki core extracted from Lake Biwa with time resolutions of approximately 150 years was used to reconstruct millennial-scale climate change in the East Asian monsoon region over the past 300 ka. The total carbon content of the Takashima-oki core is an indirect proxy for the East Asian summer monsoon, because the total carbon content reflects primary productivity within the lake, which is controlled by precipitation in the catchment area. Using these data, we clarify temporal variations in the East Asian summer monsoon prior to MIS 5, which were previously unresolved. The observed variations show a ∼1,500-year cycle that is also recorded by paleoclimate indices in the North Atlantic region. We propose that the East Asian summer monsoon underwent abrupt millennium-scale changes during interglacial stages such as MIS 5 and 7. [ABSTRACT FROM AUTHOR]

This study reconstructed environmental changes to the seafloor associated with reclamation in Mishou Bay, Bungo Channel, Japan, based on measurements of sediment grain size, organic matter and sulfur contents of surface sediments and data from sediment cores. Grain size within sediment cores from the middle of Mishou Bay decreased from the beginning of the 1800s to the 1900s. In contrast, a grain size profile from the river mouth shows a gradual increase in grain size up through the sediment core. These changes in grain size indicate a decrease in tidal current velocity within the middle of the bay and that the delta system is gradually prograding from the river mouth. Records of organic matter composition and sulfur contents indicate that the effect of the river on seafloor sedimentation became stronger during the nineteenth century. These changes are related to reclamation during the late 1700s and 1800s. The decrease in sea area resulting from reclamation probably led to a decrease in tidal prism and current velocity. It is likely that the increasing effect of river water on sedimentation is associated with reclamation-related progradation of the river delta system. [ABSTRACT FROM AUTHOR]