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In order to reduce the signal holes in wireless sensor networks, we proposed a sensor deployment density-aware signal hole repair strategy of mobile crowd network. On the one hand, based on the multi-dimensional connected graph, the density continuous jitter problem of the sensor network is solved effectively. Sensor density prediction method improves the efficiency of random deployment of sensor nodes. Based on the above schemes, the sensor deployment density sensing model is proposed. On the other hand, according to the regional geometry, diversity of the sensor network channel and density can detect the time domain channel impulse response signal successfully. Finally, the network signal is detected by the signal detection of the mobile crowd network. The signal holes can be detected and repaired based on coverage density and crowd. Experimental results show that the proposed algorithm has outstanding performance in terms of signal strength and signal void ratio compared with the energy-aware repair algorithm. [ABSTRACT FROM AUTHOR]

Xanthomonas TALE transcriptional activators act as virulence or avirulence factors by activating host disease susceptibility or resistance genes. Their specificity is determined by a tandem repeat domain. Some Xanthomonas pathogens contain 10-30 TALEs per strain. Although TALEs play critical roles in pathogenesis, their studies have so far been limited to a few examples, due to their highly repetitive gene structure and extreme similarity among different members, which constrict sequencing and assembling. To facilitate TALE studies, we developed an efficient and rapid pipeline for genome-wide cloning of tal genes as many as possible from a strain. Here, we report the pipeline and its use to identify all 18 tal genes from a newly isolated strain of the rice pathogen Xathomonas oryzae. Target prediction revealed a number of potential rice targets including several notable genes such as genes encoding SWEET, WRKY, Hen1, and BAK1 proteins, which provide candidates for further experimental functional analysis of the TALEs. [ABSTRACT FROM AUTHOR]