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Decreased adhesion of Staphylococcus aureus provides additional opportunities for the prevention and treatment of infectious diseases caused by bacteria, including drug-resistant strains. In this work, the interaction of S. aureus ATCC 29213 strain and epithelial eukaryotic cells was studied using optical microscopy. Low-molecular-mass organic compounds of the 5-arylpyrrolidine-2-carboxylate class were found to be capable of decreasing the adhesion of bacterial cells to Vero cells. Racemic methyl (2S*,4S*,5R*)-4-(hydroxymethyl)-1-methyl-5-(2-fluoro-5-methoxyphenyl)pyrrolidine-2-carboxylate produced statistically significant inhibition of the adhesion of S. aureus type strains and was characterized by a lack of cytotoxicity against both the eukaryotic Vero cell line and the studied S. aureus strain. [ABSTRACT FROM AUTHOR]

Pectic polysaccharides have been isolated from callus tissue of the stem of Sosnowskyi's hogweed (Heracleum sosnowskyi Manden) by extraction with a 0.7% aqueous solution of ammonium oxalate and characterized. It was shown that they differ from pectic polysaccharides of a native plant by a lower content of D‑galacturonic acid residues and a higher content of arabinose and galactose residues. The results obtained by a combination of ion exchange chromatography, enzymatic hydrolysis, periodate oxidation, methylation and NMR spectroscopy indicated the presence of low-methyl esterified 1,4-α-D-galacturonan, rhamogalacturonan-I, arabinan and galactan or arabinogalactan. The terminal 1,2- and 1,5-linked arabinofuranose residues, terminal, 1,3-, 1,6-linked and 3,6-substituted galactopyranose residues and 1,4-linked hexopyranose (galactopyranose or glucopyranose) residues were identified in the composition of neutral carbohydrate chains. The presence in the NMR spectra of trans-glycosyl correlation peaks of the methyl groups of 2,4-di-O-substituted rhamnose residues indirectly confirm the attachment arabinan, galactan or arabinogalactan carbohydrate chains to rhamnopyranose residues of the backbone at C-4 position. [ABSTRACT FROM AUTHOR]