1. Effect of Reversion Back to Cys11 on the Structure and Function of S11C Cys-free Nt.BspD6I.
- Author
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Artyukh, R. I., Fatkhullin, B. F., Antipova, V. N., Perevyazova, T. A., Kachalova, G. S., and Yunusova, A. K.
- Subjects
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SITE-specific mutagenesis , *ENDONUCLEASES , *CRYSTAL structure , *OLIGOMERIZATION , *DNA - Abstract
The three-dimensional structure of recombinant nicking endonuclease S11C Cys-free Nt.BspD6I was determined at 1.85 Å resolution. Nickase S11C Cys-free Nt.BspD6I was produced by the reversion back to Cys11 in Cys-free Nt.BspD6I using site-directed mutagenesis. An analysis of the crystal structure of nickase S11C Cys-free Nt.BspD6I demonstrated that the reversion back to Cys11 induces significant conformational changes in the recognition domain of nickase, which are accompanied by changes in its functions, such as a decrease in the affinity to DNA, the loss of the ability to undergo oligomerization, and high activity of restriction endonuclease S11C Cys-free R.BspD6I. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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