1. Coming together to define membrane contact sites
- Author
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György Hajnóczky, Harald Stenmark, Luca Pellegrini, Luca Scorrano, Tim P. Levine, Karin M. Reinisch, Rosario Rizzuto, Maya Schuldiner, Laura L. Lackner, Christian Ungermann, Francesca Giordano, Scott D. Emr, Thomas Simmen, Maria Antonietta De Matteis, Benoît Kornmann, Universita degli Studi di Padova, Dulbecco Telethon Institute, Telethon Foundation, Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Département Biologie Cellulaire (BioCell), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Trafic lipidique et sites de contact membranaire (COAST), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, CNR Institute of Neuroscience, University of Padova, University of Oslo (UiO), Weizmann Institute of Science [Rehovot, Israël], Scorrano, Luca, De Matteis, Maria Antonietta, Emr, Scott, Giordano, Francesca, Hajnóczky, György, Kornmann, Benoît, Lackner, Laura L., Levine, Tim P., Pellegrini, Luca, Reinisch, Karin, Rizzuto, Rosario, Simmen, Thoma, Stenmark, Harald, Ungermann, Christian, Schuldiner, Maya, and Università degli Studi di Padova = University of Padua (Unipd)
- Subjects
Genetics and Molecular Biology (all) ,0301 basic medicine ,Vocabulary ,imaging reveals ,Computer science ,[SDV]Life Sciences [q-bio] ,General Physics and Astronomy ,02 engineering and technology ,er-mitochondria ,Biochemistry ,mitochondria-associated membranes ,Animals ,Cell Fractionation ,Cell Membrane ,Eukaryotic Cells ,Humans ,Intracellular Membranes ,Microscopy ,Organelles ,Proteins ,Staining and Labeling ,Terminology as Topic ,Chemistry (all) ,Biochemistry, Genetics and Molecular Biology (all) ,Physics and Astronomy (all) ,lcsh:Science ,media_common ,Membrane contact sites ,Multidisciplinary ,Phospholipid transport ,plasma-membrane ,High capacity ,021001 nanoscience & nanotechnology ,proteomic analysis ,Knowledge production ,Mitochondria ,Perspective ,Mitochondrial Membranes ,saccharomyces-cerevisiae ,0210 nano-technology ,Plasma membrane ,media_common.quotation_subject ,Science ,rat-liver ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Cholesterol transport ,lipid-synthesis ,Mitochondria-associated membranes (MAM) ,rough endoplasmic-reticulum ,Mitochondria associated membranes ,Field (Bourdieu) ,Comment ,Biological Transport ,General Chemistry ,Lipid Metabolism ,Data science ,high-capacity ,030104 developmental biology ,Rat liver ,lcsh:Q ,Endoplasmic reticulum ,Diversity (politics) - Abstract
Close proximities between organelles have been described for decades. However, only recently a specific field dealing with organelle communication at membrane contact sites has gained wide acceptance, attracting scientists from multiple areas of cell biology. The diversity of approaches warrants a unified vocabulary for the field. Such definitions would facilitate laying the foundations of this field, streamlining communication and resolving semantic controversies. This opinion, written by a panel of experts in the field, aims to provide this burgeoning area with guidelines for the experimental definition and analysis of contact sites. It also includes suggestions on how to operationally and tractably measure and analyze them with the hope of ultimately facilitating knowledge production and dissemination within and outside the field of contact-site research., Given the recent growing interest in interorganelle membrane contact sites, the field will benefit from clear rules to define and study them. In this Perspective, a panel of experts aims to provide this growing field with guidelines for experimental definition and analysis.
- Published
- 2019