7 results on '"Dong-Mei Gao"'
Search Results
2. Comparative proteomics and molecular mechanical analysis in CDA-II induced therapy of LCI-D20 hepatocellular carcinoma model.
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Hui-zhi Fan, Hang Liu, Chen Zhang, Dong-mei Gao, Qun Xue, Jun Chen, Rui-xia Sun, Yin-kun Liu, and Peng-yuan Yang
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LIVER cancer ,METASTASIS ,TUMORS ,CELL differentiation ,MASS spectrometry - Abstract
To investigate the differential proteins and related molecular mechanism of CDA-II (cell differentiation agent-II) induced therapy on a human hepatocellular carcinoma model in nude mice with high metastatic potential (LCI-D20). After tumors were transplanted 11 days, mice were intraperitoneally injected with CDA-II (1,800 mg/kg) for 20 days continuously. The tumor growth-inhibitory efficiency in CDA-II treated groups was calculated. Proteins extracted from tumor tissue were separated by two-dimensional gel electrophoresis (2DE) and the differential proteins were identified by matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS). Western blotting (WB) was performed to verify the expression of certain candidate proteins. Reverse transcription-polymerase chain reaction (RT-PCR) was engaged to study the molecular mechanism of the therapy. CDA-II suppressed the growth and metastasis of tumor. The tumor growth-inhibitory efficiency was 41.8%. In total, 27 differentially expressed proteins were identified, including HSP27, UGDH, CK8, Hsp60, ENOA and AnxA5, with functions involved in oncogene expression and/or cell differentiation. In addition, apparent alternations of HSP60 and β-actin expression levels and their different posttranslational modifications (PTMs) were investigated. RT-PCR analysis confirmed that the cancer related genes c-myc, N-ras and MMP-9 were significantly down-regulated. Our results demonstrate that CDA-II presence can change the proteome profiling and favors of the tumor suppression in LCI-D20 cell differentiation. Our results also suggest that the dynamic PTM of HSP60 expression levels could be used to predict HCC and might be a promising and useful biomarker to prognosticate CDA-II therapeutic efficacy. [ABSTRACT FROM AUTHOR] more...
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- 2009
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Catalog
3. Determination of hemoglobin at a novel NH2/ITO ion implantation modified electrode.
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Dong Mei Gao, Yuan Yuan Sun, Qiongyan Zhao, Jing Bo Hu, and Qi Long Li
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HEMOGLOBINS , *ION implantation , *ELECTRODES , *NANOPARTICLES , *NANOSTRUCTURED materials - Abstract
A novel electrode was prepared by implanting NH2 + into an ITO film (NH2/ITO). Gold nanoparticles were deposited on the surface of NH2/ITO electrode. The NH2/ITO and Au/NH2/ITO electrodes were used to determine hemoglobin (Hb) immobilized on the electrodes surfaces. The relationship of the reductive peak current value of Hb among different electrodes was: Hb/ITO:Hb/Au/ITO:Hb/NH2/ITO:Hb/Au/NH2/ITO=1:1.5:2:4. The linkage between the –NH2 implanted into ITO film and the –COOH of Hb was recognized to be the reason for the increase of active Hb coverage on NH2/ITO electrode compared with the ITO electrode. Increase of active Hb coverage on Au/NH2/ITO compared with Au/ITO was attributed to the different amount of gold nanoparticles deposited. The determination of Hb at an Au/NH2/ITO electrode was optimized. Calibration curve was obtained over the range of 1.0 × 10−8 – 1.0 × 10−6 mol · L−1 with a detection limit of 1.0 × 10−8 mol · L−1. Results showed that the novel NH2/ITO and Au/NH2/ITO electrodes exhibited good stability, reproducibility besides better electrochemical performance. [ABSTRACT FROM AUTHOR] more...
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- 2008
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4. Establishment of green fluorescent protein-expressing hepatocellular carcinoma cell lines with different metastatic potential: relevant models for in vivo monitoring of metastasis and angiogenesis.
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Yang Xu, Hui-Chuan Sun, Bo Tian, Yan Li, Jie Chen, Jun Chen, Dong-Mei Gao, Qiong Xue, and Zhao-You Tang
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GREEN fluorescent protein ,PROTEINS ,FLUORESCENT polymers ,LIVER cancer ,METASTASIS ,NEOVASCULARIZATION - Abstract
Purpose. To establish stable green fluorescent protein (GFP)-expressing metastatic human hepatocellular carcinoma (HCC) cell lines with different metastatic potential for long-term in vivo studies of metastasis and angiogenesis. Methods. The pIRES2-EGFP vector, which contains an enhanced GFP gene, was transfected into MHCC97-H and MHCC97-L, HCC cell lines with different metastatic potential. The stability of GFP expression, basic biological characteristics, invasion abilities in vitro, and spontaneous metastasis in vivo of the new cell lines (MHCC97-HG and MHCC97-LG) were studied. Microvessel density (MVD) of orthotopic implanted tumors was compared by anti-CD31 immunohistochemical staining, and real-time angiogenesis and metastasis of GFP-transfected tumors were detected by intravital fluorescent microscope. Results. The GFP-transfected cell lines stably expressed green fluorescence in the absence of G418 over a 36-day period. Compared with the parental cell lines, they exhibited no distinct differences in biological characteristics. MHCC97-HG showed more aggressive invasion and spontaneous metastatic behavior than MHCC97-LG, and even its parental cell line, MHCC97-H (P<0.01). MVD levels induced by MHCC97-HG orthotopic implanted tumors were significantly higher than MHCC97-LG (P<0.01). Real-time angiogenesis and sequential steps of metastasis could be detected clearly under intravital fluorescent microscope. Conclusions. These two stable GFP-expressing HCC cell lines with the same genetic background and different metastatic potential were established, which could be useful models for monitoring metastasis and angiogenesis of HCC. [ABSTRACT FROM AUTHOR] more...
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- 2004
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5. Contributions of lung tissue extracts to invasion and migration of human hepatocellular carcinoma cells with various metastatic potentials.
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Xue-Ning Ji, Sheng-Long Ye, Yan Li, Bo Tian, Jie Chen, Dong-Mei Gao, Jun Chen, Wei-Hua Bao, Yin-Kun Liu, and Zhao-You Tang
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LIVER cancer ,SPLEEN ,LYMPHOID tissue ,LIVER metastasis ,CELL culture ,CANCER invasiveness - Abstract
Purpose. The MHCC97 cell line contains two clones with high (MHCC97-H) and low (MHCC97-L) metastatic potentials, for which the pulmonary metastatic rate was 100% vs 40% between the two human hepatocellular carcinoma (HCC) clones. In an effort to elucidate the mechanism of organ-specific metastasis, we studied the effect of lung extracts from C57BL/6 mice on migration and invasion using the MHCC97 cell line. Methods. Determination of migration and invasion induced by lung extracts to MHCC97 cell lines was examined by chemoinvasion assay. The organization of cytoskeleton was tested using filamentous actin (F-actin) polymerization assay and flow cytometry. The activity of matrix metalloproteinase (MMPs) was analyzed by zymogram. Fluorescence double staining was employed for MMPs and F-actin colocalization in MHCC97-H cells induced by lung extracts. Results. The number of cells in response to extracts of lung, liver, kidney, and spleen in MHCC97-H cells was 64±10, 6±2, 22±4, and 3±1, respectively. Of the extracts, lung extracts showed significant differences to promote the migratory and invasive ability for MHCC97-H cells(p<0.001). The number of cells in response to lung extracts was threefold higher in MHCC97-H than that of cells in MHCC97-L (p<0.001). Confocal laser scan microscope of MHCC97-H cells and MHCC97-L cells stimulated with lung extracts revealed pseudopodia formation around the cell front at the indicated time point. With the time increasing, the pseudopodia formation became increasingly more obvious and distinct. Compared with unstimulated cells, analysis of FACS showed a transient 1.9-fold and 1.7-fold increase in F-actin within 30 s in MHCC97-H cells and MHCC97-L cells, respectively. Confocal laser scan microscopy of MHCC97-H cells stimulated in suspension with lung extracts revealed intense F-actin staining in the periphery of the cells and redistribution of F-actin towards a leading edge. After the cells were incubated with lung extracts, not only expressions of active and latent form of MMP-9 were upregulated, but that of latent form of MMP-2 was increased in the MHCC97-H cells and MHCC97-L cells. The levels of latent and active form of MMP-9(18.8±1.2, 100.1±1.1), and latent form of MMP-2(22.4±1.3) were much higher in MHCC97-H cells than those of MHCC97-L cells, which were 7.8±0.3, 40.8±2.2, and 8.2±0.4, respectively. MMP-9 was mainly localized perinuclear pool when the MHCC97-H cells were incubated with serum-free medium. After the cells were stimulated with lung extracts, MMP-9 were expressed and colocalized with F-actin at the front of extending pseudopodia. Conclusions. Our results indicate that the expression of matrix metalloproteinase and the pseudopodia formation in MHCC97-H cells may correlate to the metastatic potential; thus, the host environment may contribute to the preferential metastasis of HCC cells to the lung depending on the high level of MMP-9 and migratory ability. [ABSTRACT FROM AUTHOR] more...
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- 2003
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6. The abnormalities of chromosome 8 in two hepatocellular carcinoma cell clones with the same genetic background and different metastatic potential.
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Jiong Yang, Lun-Xiu Qin, Sheng-Long Ye, Yin-Kun Liu, Yan Li, Dong-Mei Gao, Jie Chen, and Zhao-You Tang
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LIVER cancer ,CHROMOSOMES ,CHROMOSOME abnormalities ,METASTASIS ,COMPARATIVE genomic hybridization ,ONCOLOGY ,GENETICS ,GENOMICS - Abstract
Purpose. Two hepatocellular carcinoma (HCC) cell clones named MHCC97-H and MHCC97-L with different metastatic potential have recently been established from the same parent cell line MHCC97 in our institute. The cytogenetic alterations of these two clones were investigated in this study to explore the possible clues to the mechanism involved in HCC metastasis. Methods. Their chromosomal aberrations were analyzed with comparative genomic hybridization (CGH), chromosome-specific painting, and two-color fluorescence in situ hybridization (FISH). Results. The aberrations were found in a total of 17 chromosomes, and six kinds of the aberrations including gains of 1q, 7q, 8q, 20, and the losses of 8p23, 21q were found both in the two cell clones and their parent cell line MHCC97. Using modified CGH, with the DNA of MHCC97-L as control to test the MHCC97-H clone, the loss of 8p23 and the gain of 1q31–32, 8q21.3–22, 13q22, 17q22 were highlighted, and the most significant finding was on chromosome 8. Dual color FISH combining a pericentromeric probe and a BAC probe mapping at 8q23.1 was then performed to verify this result, and the signal ratios of the BAC to centromere were 1.43 in MHCC97-H and 1.45 in MHCC97-L, confirming the over-representations at 8q in both cells. Another interesting finding in the dual-color metaphase FISH was the intrachromosomal translocation of 8q to 8p (looked like an isochromosome 8) and non-reciprocal translocation of part of 8q to 4q, which was further clarified and proved by the FISH with whole chromosome 8 painting probe. Conclusions. The high copies amplification on 8q, the formation of isochromosome 8, non-reciprocal translocation of partial 8q to 4q, and loss of 8p occurred at the same time and are the characteristic chromosomal aberrations of the two cell clones. The chromosome 8p, especially 8p23, might harbor some novel genes related to the HCC metastasis. [ABSTRACT FROM AUTHOR] more...
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- 2003
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7. Antiangiogenic effects of pazopanib in xenograft hepatocellular carcinoma models: evaluation by quantitative contrast-enhanced ultrasonography
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Wei-Zhong Wu, Pei-li Fan, Hua-Xiang Xu, Ju-Bo Zhang, Yuquan Xiong, Peng-Yuan Zhuang, Zhao-You Tang, Hui-Chuan Sun, Ling-Qun Kong, Dong-Mei Gao, Hong Ding, Wei Zhang, Xiao-Dong Zhu, and Lu Wang
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Male ,Pathology ,medicine.medical_specialty ,Cancer Research ,Carcinoma, Hepatocellular ,Indazoles ,Cell Survival ,Mice, Nude ,Angiogenesis Inhibitors ,Apoptosis ,lcsh:RC254-282 ,Cell Line ,Pazopanib ,Mice ,Liver Neoplasms, Experimental ,Surgical oncology ,Cell Line, Tumor ,medicine ,Carcinoma ,Genetics ,Animals ,Humans ,Cells, Cultured ,Cell Proliferation ,Ultrasonography ,Mice, Inbred BALB C ,Sulfonamides ,Dose-Response Relationship, Drug ,Cell growth ,business.industry ,Hep G2 Cells ,Image Enhancement ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,Xenograft Model Antitumor Assays ,digestive system diseases ,Tumor Burden ,Pyrimidines ,Oncology ,Hepatocellular carcinoma ,Cancer research ,Blood Vessels ,Experimental pathology ,Stem cell ,business ,Research Article ,medicine.drug - Abstract
Background Antiangiogenesis is a promising therapy for advanced hepatocellular carcinoma (HCC), but the effects are difficult to be evaluated. Pazopanib (GW786034B) is a pan-vascular endothelial growth factor receptor inhibitor, the antitumor effects or antiangiogenic effects haven't been investigated in HCC. Methods In vitro direct effects of pazopanib on human HCC cell lines and endothelial cells were evaluated. In vivo antitumor effects were evaluated in three xenograft nude mice models. In the subcutaneous HCCLM3 model, intratumoral blood perfusion was detected by contrast-enhanced ultrasonography (CEUS), and serial quantitative parameters were profiled from the time-intensity curves of ultrasonograms. Results In vitro proliferation of various HCC cell lines were not inhibited by pazopanib. Pazopanib inhibited migration and invasion and induced apoptosis significantly in two HCC cell lines, HCCLM3 and PLC/PRF/5. Proliferation, migration, and tubule formation of human umbilical vein endothelial cells were inhibited by pazopanib in a dose-dependent manner. In vivo tumor growth was significantly inhibited by pazopanib in HCCLM3, HepG2, and PLC/PRF/5 xenograft models. Various intratumoral perfusion parameters changed over time, and the signal intensity was significantly impaired in the treated tumors before the treatment efficacy on tumor size could be observed. Mean transit time of the contrast media in hotspot areas of the tumors was reversely correlated with intratumoral microvessel density. Conclusions Antitumor effects of pazopanib in HCC xenografts may owe to its antiangiogenic effects, and the in vivo antiangiogenic effects could be evaluated by quantitative CEUS. more...
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