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Ecological water replenishment is considered to be an important project to adjust river ecosystems with water shortages and degradation, and its impact on the water quality of the target river section deserves attention. By investigating the characteristics of inorganic ions and organic components of the Beijing section of the Yongding River (YDR) from upstream to downstream, the sources of inorganic ions and dissolved organic matter (DOM) during an ecological water replenishment event were analysed and discussed. This study illustrated the hydrochemical response to different supplemental water sources in three sections of the YDR (mountain gorge section (MGS), urban plain section (UPS), and suburb plain section (SPS)). The results showed that the total dissolved solids (TDS) and ion concentrations were significantly different (p < 0.001) in the three river sections due to different supplemental water sources. The highest concentration of TDS was found in the UPS (870.92 mg/L) replenished by reclaimed water, while the lowest concentration of TDS was found in the SPS (306.95 mg/L) replenished by the water of the South-to-North Water Diversion Project (SNWD). Despite the differences in the water sources of replenishment, the optical parameters of DOM and fluorescent components showed similar protein-like dominated endogenous source characteristics in the three river sections of the YDR, which was due to the influence of reservoir water (MGS and SPS) and reclaimed water (UPS). Our study emphasizes the importance of understanding the impact of different water sources on the water replenishment process, which provides a scientific reference for the management of ecological water replenishment. [ABSTRACT FROM AUTHOR]

Petroleum hydrocarbons are hazardous to ecosystems and human health, commonly containing n-alkanes and polycyclic aromatic hydrocarbons. Previous researches have studied alkane degraders and degrading genes under aerobic or anaerobic conditions, but seldom discussed them in the intermittent saturation zone which is a connective area between the vadose zone and the groundwater aquifer with periodic alteration of oxygen and moisture. The present study investigated the difference in alkane degradation efficiency, bacterial community, and alkane degrading gene diversity in aerobic, anaerobic, and aerobic-anaerobic fluctuated treatments. All biotic treatments achieved over 90% of n-alkane removal after 120 days of incubation. The removal efficiencies of n-alkanes with a carbon chain length from 16 to 25 were much higher in anaerobic scenarios than those in aerobic scenarios, explained by different dominant microbes between aerobic and anaerobic conditions. The highest removal efficiency was found in fluctuation treatments, indicating an accelerated n-alkane biodegradation under aerobic-anaerobic alternation. In addition, the copy numbers of the 16S rRNA gene and two alkB genes (alkB-P and alkB-R) declined dramatically when switched from aerobic to anaerobic scenarios and oppositely from anaerobic to aerobic conditions. This suggested that water level fluctuation could notably change the presence of aerobic alkane degrading genes. Our results suggested that alkane degradation efficiency, soil microbial community, and alkane-degrading genes were all driven by water level fluctuation in the intermittent saturation zone, helping better understand the effects of seasonal water table fluctuation on the biodegradation of petroleum hydrocarbons in the subsurface environment. [ABSTRACT FROM AUTHOR]

Riparian vegetation cover is significantly affected by a river’s hydrological conditions. Especially in arid and semiarid areas, low flow will degrade riparian vegetation, and recent, intensive human activities in the Yongding River Basin have caused a sharp decrease in river flow. We analyzed interannual change in riparian vegetation, river flow effects, and land use on vegetation coverage using the 40 years (1977–2016) of remote sensing images and river flow, combined with 38 years (1980–2018) of land use data. The normalized difference vegetation index (NDVI) was used to determine vegetation cover in five different categories: extremely low, low, medium, high, and extremely high based on the pixel dichotomy model. The weighted average was calculated to obtain vegetation cover trends. We show that riparian vegetation cover from four rivers increased. Compared with 1977, in 2016, combined high and extremely high vegetation covers at the Dongyang, Yang, Sanggan, and Yongding Rivers increased by 20.3%, 26.7%, 50.0%, and 39.2%, respectively. High (R = −0.976, P < 0.01) and extremely high (R = −0.762, P < 0.05) vegetation covers are negatively correlated with flow in the Yongding River. The high vegetation cover of the Sanggan River riparian zone is negatively correlated with river flow (R = −0.683, P < 0.05). In the Dongyang and Sanggan Rivers, land use analysis in the riparian zone showed that change in cultivated land, grassland, and forest were significantly correlated with high and extremely high vegetation cover. The abundant cultivated land and restoration activities are likely responsible for the increase of riparian vegetation cover as river flows decline. [ABSTRACT FROM AUTHOR]

Human activities impose important disturbances on both organic and inorganic chemistry in fluvial systems. In this study, we investigated the intra-annual and downstream variations of dissolved organic carbon (DOC), dissolved organic matter (DOM) excitation-emission matrix fluorescence (EEM) with parallel factor analysis (PARAFAC), major ions, and dissolved inorganic nitrogen (DIN) species in a mountainous tributary of the Yellow River, China. Both DOM quantity and quality, as represented by DOC and DOM fluorescence respectively, changed spatially and seasonally in the studied region. Fluorescence intensity of tryptophan-like components (C3) were found much higher at the populated downstream regions than in the undisturbed forested upstream regions. Seasonally, stronger fluorescence intensity of protein-like components (C3 and C4) was observed in the low-flow period (December) and in the medium-flow period (March) than in the high-flow period (May), particularly for the downstream reaches, reflecting the dominant impacts of wastewater pollution in the downstream regions. In contrast to the protein-like fluorescence, humic-like fluorescence components C1 and C2 exhibited distinctly higher intensity in the high-flow period with smaller spatial variation indicating strong flushing effect of increasing water discharge on terrestrial-sourced humic-like materials in the high-flow period. Pollution-affected dissolved inorganic ions, particularly Na+, Cl−, and NH4+-N, showed similar spatial and seasonal variations with protein-like fluorescence of DOM. The significant positive correlations between protein-like fluorescence of DOM and pollution-affected ions, particularly Na+, Cl−, and NH4+-N, suggested that there were similar pollution sources and transportation pathways of both inorganic and organic pollutants in the region. The combination of DOM fluorescence properties and inorganic ions could provide an important reference for the pollution source characterization and river basin management. [ABSTRACT FROM AUTHOR]

High-molecular-weight polycyclic aromatic hydrocarbons are persistent organic pollutants with great environmental and human health risks and the associated bioremediation activities have always been hampered by the lack of powerful bacterial species under redox conditions. A Microbacterium sp. strain capable of using benzo(a)pyrene as sole carbon and energy sources under denitrifying conditions was isolated. The difference in protein expression during BaP removal and removal characterization were investigated. A total of 146 proteins were differentially expressed, 44 proteins were significantly up-regulated and 102 proteins were markedly down-regulated. GO and COG analysis showed that BaP removal inhibited the expression of proteins related to glucose metabolism at different levels and activated other metabolic pathway. The proteins associated with catalytic activity and metabolic process were altered significantly. Furthermore, the BaP removal might be occurred in certain organelle of M.CSW3. The strain removed BaP with a speed of 0.0657-1.0072 mg/L/day over the concentrations range 2.5-100 mg/L. High removal rates (>70%) were obtained over the range of pH 7-11 in 14 days. Carbohydrates and organic acids which could be utilized by the strain, as well as heavy metal ions, reduced BaP removal efficiency. However, phenanthrene or pyrene addition enhanced the removal capability of M.CSW3. The strain was proved to have practical potential for bioremediation of PAHs-contaminated soil and this study provided a powerful platform for further application by improving production of associated proteins. [ABSTRACT FROM AUTHOR]

This study focused on the protein expression of a Microbacterium sp. strain that utilized various concentrations of benzo(a)pyrene (BaP) as the sole source of carbon and energy under anaerobic conditions. A total of 1539 protein species were quantified by isobaric tags for relative and absolute quantitation (iTRAQ) coupled with LC-MS/MS. GO, COG, and pathway enrichment analysis showed that most proteins demonstrated catalytic and binding functions and were mainly involved in metabolic processes, cellular processes, and single-organism processes. Sixty-two proteins were found in their abundances in BaP-stress conditions different from normal conditions. These proteins function in the metabolic pathways; the biosynthesis of secondary metabolites, the biosynthesis of antibiotics, microbial metabolism in diverse environments, carbon metabolism, and the biosynthesis of amino acids were markedly altered. Furthermore, enoyl-CoA hydratase was proposed to be a key protein during BaP removal of the Microbacterium sp. strain. This study provides a powerful platform for the further exploration of BaP removal, and the differentially expressed proteins provide insight into the mechanism of the BaP removal pathway. [ABSTRACT FROM AUTHOR]

Potential threats exist where groundwater is polluted by high concentrations of oil compounds (980.20 mg L the highest TPHs). An abandoned petrochemical plant in Lanzhou City, where long-term petrochemical products leakage contaminated the groundwater, was used as a field site in this study. To determine the extent of pollution and find an effective solution, chemical techniques combined with molecular biological techniques were used to survey the migration and decomposition of pollutants. Moreover, Illumina Sequencing was employed to reveal the microbial changes of different sites. Light-chain alkanes (mostly C6-C9), most benzene compounds, and some polycyclic aromatic hydrocarbons (naphthalene, 2-methylnaphthalene) mainly polluted the source. C29 to C36 and chlorobenzenes (hexachlorocyclohexane) polluted the secondary polluted sites. Moreover, chloralkane (trichloroethane and dichloroethane), benzene derivatives (trimethylbenzene and butylbenzene), and PAHs (fluorene and phenanthrene) were present in the other longtime-contaminated water. The bacterial genera are closely related with the chemical matters, and different groups of microorganisms gather in the sample sites that are polluted with different kinds of oil. The biodiversity and abundance of observed species change with pollution conditions. The dominant phyla (81%) of the bacterial community structure are Proteobacteria (62.2% of the total microbes), Bacteroidetes (8.85%), Actinobacteria (6.70%), and Choloroflexi (3.03%). Pseudomonadaceae is significant in the oil-polluted source and Comamonadaceae is significant in the secondary polluted (migrated oil) sample; these two genera are natural decomposers of refractory matters. Amycolatopsis, Rhodocyclaceae, Sulfurimonas, and Sulfuricurvum are the dominant genera in the long-migrated oil-polluted samples. Bioavailability of the oil-contaminated place differs with levels of pollution and cleaning the worse-polluted sites by microbes is more difficult. [ABSTRACT FROM AUTHOR]

The connection between microbial community structure and spatial variation and pollution in river waters has been widely investigated. However, water and sediments together have rarely been explored. In this study, Illumina high-throughput sequencing was performed to analyze microbes in 24 water and sediment samples from natural to anthropogenic sources and from headstream to downstream areas. These data were used to assess variability in microbial community structure and diversity along in the Fenghe River, China. The relationship between bacterial diversity and environmental parameters was statistically analyzed. An average of 1682 operational taxonomic units was obtained. Microbial diversity increased from the headstream to downstream and tended to be greater in sediment compared with water. The water samples near the headstream endured relatively low Shannon and Chao1 indices. These diversity indices and the number of observed species in the water and sediment samples increase downstream. The parameters also differ in the two river tributaries. Community structures shift based on the extent of nitrogen pollution variation in the sediment and water samples. The four most dominant genera in the water community were Escherichia, Acinetobacter, Comamonadaceae, and Pseudomonas. In the sediments, the most dominant genera were Stramenopiles, Flavobacterium, Pseudomonas, and Comamonadaceae. The number of ammonia-oxidizing archaea in the headstream water slightly differed from that in the sediment but varied considerably in the downstream sediments. Statistical analysis showed that community variation is correlated with changes in ammonia nitrogen, total nitrogen, and nitrate nitrogen. This study identified different microbial community structures in river water and sediments. Overall this study emphasized the need to elucidate spatial variations in bacterial diversity in water and sediments associated with physicochemical gradients and to show the effects of such variation on waterborne microbial community structures. [ABSTRACT FROM AUTHOR]

The formation factor relates bulk resistivity to pore fluid resistivity in porous materials. Understanding the formation factor is essential in using electrical and electromagnetic methods to monitor leachate accumulations and movements both within and around landfills. Specifically, the formation factor allows leachate resistivity, the degree of saturation, and, possibly, even the hydraulic conductivity of the waste to be estimated from non-invasive surface measurements. In this study, apparent formation factors are computed for three landfills with different types of waste as well as sediments contaminated by landfill leachate. Resistivity soundings at the closed Mallard North landfill in suburban Chicago (Illinois, USA) mapped leachate surfaces that were confirmed by monitoring wells. The resistivity of leachate-saturated waste from resistivity sounding inversions was then divided by the leachate resistivity values measured in-situ to compute apparent formation factors ( F a) ranging from 1.6 to 4.9. A global F a of 3.0±1.9 was computed for the entire monitored portion of this landfill. At a nearby mixed laboratory waste landfill, a 2D inverted resistivity section was used to compute an F a of 2.9. Finally, a distinctly different F a value of 10.6±2.8 was computed for leachate-saturated retorted oil shale wastes north of Maoming (茂名), Guangdong (广东) Province, China. Shallow aquifers in the Laohuling (老虎岭) Formation near this landfill are polluted by acidic leachate containing heavy metals and organic compounds. The F a for aquifers containing contaminated groundwater fall in the same range as aquifers with normal groundwater, 1.7–3.9. However, models from inverted sounding curves over these contaminated areas exhibit unusually low resistivity layers, which may be diagnostic of contamination. [ABSTRACT FROM AUTHOR]

One strain of photosynthetic bacteria (PSB) was isolated from substrate sludge of fresh-water fishpond. Influence of the use level of PSB culture solution, illumination condition, temperature, salinity, the use level of copper sulfate and dipterex on the purification efficiency was investigated. The results showed that the optimum use level of PSB culture solution was 10 mg/L, and the purification efficiency at illumination was higher than that at black, and if the temperature was lower than 15 °C., or the use level of sodium chloride, copper sulfate and dipterex were higher than 10 000 mg/L, 0.4 mg/L and 2.0 mg/L, respectively, the purification efficiency dropped distinctly. [ABSTRACT FROM AUTHOR]

Nitrate pollution of groundwater is paid more and more attention for its hazardous to environments and human health. A strain of DN11 was isolated from soil and used in the laboratory columns filled with various media for nitrate removal. The experimental results showed that DN11 could reduce nitrate at different rates in different media under the aerobic condition. The mechanism for nitrate removal with DN11 is explained meanwhile. [ABSTRACT FROM AUTHOR]