Search

Background: With 9.6 million deaths in 2018, cancer remains the second leading cause of death worldwide. Breast cancer is the most deadly type of cancer among females, with 55.2% of crude incidence rate and 16.6% of crude mortality rate. Purpose: The present study was aimed to investigate the anti-breast cancer potential of natural dietary flavonoid, apigenin isolated from Clerodendrum viscosum leaves. Methods: Apigenin was evaluated for in-depth anticancer activity in MCF-7 cells using cell viability assay, cell cycle analysis, Annexin-V-FLUOS staining, ROS induction, morphological analysis, and western blot analysis. Results: Apigenin showed selective cytotoxicity on MCF-7 cells with an IC50-56.72 ± 2.35 µM, while negligible cytotoxicity was observed on WI-38 cells. Further, the flow cytometer-based analysis showed that apigenin halted MCF-7 cells in the G2/M phase arrest followed by dose-dependent apoptosis. Moreover, the FACS and confocal microscopy results confirmed the elevation of intracellular ROS and nuclear fragmentation in apigenin-treated MCF-7 cells. Western blots showed up-regulation of cell cycle regulatory proteins, increased p53 expression, Bax/Bcl-2 ratio, activation of caspases, and cleavage of PARP. Finally, apigenin treatment in the presence of Pifithrin-µ showed decreased apoptotic population and it was further confirmed through western blotting study. The results revealed the vital role of p53 in apigenin-induced apoptosis in MCF-7 cells. Conclusions: In the present findings, treatment of apigenin-induced intracellular ROS in MCF-7 cells followed by induction of G2/M phase cell cycle arrest and further apoptosis through the regulation of p53 and caspase-cascade signaling pathway. [ABSTRACT FROM AUTHOR]

Obesity-related hypertension is associated with increased activity of the renin-angiotensin-aldosterone system (RAAS), increasing arterial stiffness. Aerobic exercise decreases pulse wave velocity (PWV), therefore a treatment option for hypertension and obesity. Assess RAAS activity and PWV before and after 4 weeks of aerobic training in unmedicated, pre-to-stage-1 hypertensives. Ten obese subjects (52±3.2 years, body mass index=33.5±1.4) performed 30 min of aerobic exercise on a treadmill 3 days per week at 65% of peak oxygen consumption (VO2peak). Descriptive characteristics, systolic and diastolic blood pressure (SBP and DBP), PWV, and a blood draw was performed at baseline, following the 4-week control and training interventions. No differences in descriptive characteristics during the control period were observed, however, a significant decrease in plasma aldosterone (ALDO) (255.4±75 to 215.8±66 pg ml−1, P=0.001), SBP (140±12 to 136±10.4 mm Hg; P=0.02), DBP (89±4.2 to 85±6.3 mm Hg; P=0.03) and central PWV (11.2±0.6 to 9.8±0.8 m s−1; P=0.04) was shown pre-to-post exercise training. Four weeks of moderate-intensity aerobic training in obese, hypertensives decreases plasma ALDO independently of body weight and is significantly correlated to decreases in PWV reductions. [ABSTRACT FROM AUTHOR]

A new method to study families of finite sets, in particular t-designs, by studying families of multisets (also called lists) and their relationships with families of sets, is developed. Notion of the tag for a subset defined earlier by one of the authors is extended to a submultiset. A new concept t-( v, k, λ) list design is defined and studied. Basic existence theory for designs is extended to a new set up of list designs. In particular tags are used to prove that signed t-list designs exist whenever necessary conditions are satisfied. The concepts of homomorphisms and block spreading are extended to this new set up. [ABSTRACT FROM AUTHOR]

In this paper, we have presented a new method for computing the best-fitted rectangle for closed regions using their boundary points. The vertices of the best-fitted rectangle are computed using a bisection method starting with the upper-estimated rectangle and the under-estimated rectangle. The vertices of the upper- and under-estimated rectangles are directly computed using closed-form solutions by solving for pairs of straight lines. Starting with these two rectangles, we solve for the best-fitted rectangle iteratively using a bisection method. The algorithm stops when the areas of the fitted rectangles remain unchanged during consecutive iterations. Extensive evaluation of our algorithm demonstrates its effectiveness. [ABSTRACT FROM AUTHOR]

Two designs of sugarcane cutter planters namely IISR Lucknow designed ITI make and Khalsa PE 630 were evaluated under laboratory and field conditions. Set cutting and fertilizer metering mechanism were evaluated under laboratory conditions. It was found that cutting force required to cut sugarcane setts varied form 12.2 to 106.57 N depending upon diameter of cane. The cutting force varied 29.14 to 106.57 N in cylindrical cutting mechanism used in Khalsa PE 630 planters and 12.2 to 81.20 in rotary cutting mechanism used in IISR planter. Evaluation of fertilizer metering mechanism showed that there were wide variations in quantity of fertilizer metered at different settings. The recommended doses of fertilizers could not be metered in many cases. Intensive field evaluation of both planters was carried out in different parts of the country. The field capacity of the planter varied from 0.08 — 0.22 ha/h and field efficiency 30.61 — 71.33 %. Depth of planting varied form 12 — 25 cm. Set length was 30 — 50 cm and bud damage was less than 5%. Refinements were carried out in the sugarcane cutter planters, which included adoption of screw conveyor metering system for fertilizer and refinements in blade geometry of the set cutting unit. In addition a two row adjustable spacing 90, 120 and 150 cm sugarcane cutter planter having force feed rotary cutting system was also developed. [ABSTRACT FROM AUTHOR]

The influence of metals, Cd, Cr, Cu, Ni, Pb and Zn, on the microbial biomass and enzyme activities of an amended acid lateritic soil were investigated under field conditions receiving a one-time application of 52 t ha-1 of sludge, coal ash and their mixtures at 1:3, 1:1 and 3:1 proportions, and including control and chemical fertilizer treatment at crop-specific recommended doses. Paddies and peanuts were grown in the experimental plots and soil was sampled twice after 6 months and 1 year after amendment application. The heavy metals in the soil were fractionated using sequential extraction and the increments in their concentrations in amended soil with respect to the control were determined. Concentrations of Cd, Ni and Zn were determined to have increased in their mobile fractions and were more pronounced in soil collected during the second sampling, which was associated with a decrease in soil organic carbon. The size of the microbial biomass carbon and the soil enzyme activities increased with the addition of an amendment and was highest at equal proportions of coal ash and sludge. Further increase in the proportion of sludge resulted in a significant decrease in biomass carbon. Simple correlation revealed significant and strong negative relations of mobile fractions of Cd and Ni with the ratio between microbial biomass C and organic carbon in soil, while the organic carbon content and the pH were positively correlated. The microbial activities were determined to be sensitive to the concentrations of some heavy metals in mobile fractions and therefore indicated possibilities of being useful as indicators for evaluation of toxic effects of sludge-borne metals on soil organisms. [ABSTRACT FROM AUTHOR]

A sequential extraction procedure has been used to study the changes in the distribution and mobility of Cd, Cr, Cu, Ni, Pb and Zn in an acid lateritic soil amended with alkaline coal ash and neutral sludge individually and with their mixture of equal proportions at 25, 50 and 75 Mg/ha application rates and grown in a crop with peanuts. A separate set of experiments consisting of the same treatments was repeated with the addition of lime at 2 Mg/ha. Increases in total heavy-metal levels with application of various amendments were mainly associated with increases in the insoluble and less mobile forms of metals except for Cd, which showed an increase in its exchangeable form. An increase in pH of the amended soil restricted the metal mobility in their labile forms and was more pronounced after liming the treatments. Positive yield responses were observed in the amended soil, the magnitude being higher in only sludge-applied treatments. The vegetative plant parts showed maximum accumulation of metals indicating a physiological barrier in the transfer of metals from the root to the kernel. Linear relationships of total concentrations of heavy metals in soil with that in the crop were observed. Lime addition to the treatments further reduced the transfer and accumulation of metals from the soil to the plant, even though the relationship remained linear. [ABSTRACT FROM AUTHOR]

Laboratory batch experiments were carried out to study the competitive sorption behavior of metals in three types of Indian soils, differing in their physicochemical properties: acid laterite (SL1), red alfisol (SL2) and black vertisol (SL3) treated with different proportions of fly ash and sewage sludge mixture. Representative samples were equilibrated with 10 to 200 µM L-1 concentrations of metals simultaneously containing Cd, Cu, Ni, Pb and Zn in 5 mM of Ca(NO3)2 solution. In most of the cases the affinity sequence of metals was Pb>Cu>Zn>Ni>Cd based on their amount of sorption, which varied little with either metal equilibrating concentrations or the soil/mixture type. The observed metal affinity sequences in different soils amended with mixtures were compared to the predicted affinity sequences based on metal properties and a good match was found with those predicted by metal hydrolysis constants. This indicated that formation and subsequent sorption of metal hydrolysis products on soil surface is the predominant mechanism for sorption. In all the cases, Pb and Cu showed higher affinity followed by Zn, Ni or Cd. The increase in the metal additions further enhanced the competition among metals for exchange sites. Adsorption isotherms showed that metal sorption was linearly related to its concentration in the equilibrium solution. The distribution coefficients (KD) computed from the slopes of linear regression for different metals were higher in SL3 than in both SL2 and SL1. All the mixture amended soils produced higher KD values than their respective controls. Selectivity between metals resulted in the following affinities based on their KD values—Pb>Cu>Zn>Ni or Cd—which was in line with the value of the hydrolysis constant of the metals under study. [ABSTRACT FROM AUTHOR]

The use of coal fly ash and domestic sewage sludge in agriculture is being considered as one of the methods for recycling of these wastes in an environmental beneficial manner. Mixtures with soil were prepared at different proportions of fly ash and sludge, either alone or in combination at a maximum application rate of 52 t ha–1. The changes in the selected properties and heavy metal contents of three soil types in India were studied after incubating the respective mixtures for 90 days at near field capacity moisture level. Sewage sludge, due to its acidic and saline nature, high organic matter and heavy metals content, had more impact on soil properties than the fly ash. Sludge application produced several changes including an increase in available nitrogen, organic carbon, salinity and water-holding capacity of the soils. The concentrations of major cations and heavy metals also increased because of the sludge application and the pH was decreased. However, the levels of individual metal concentrations in all the mixture types were below the allowable limits prescribed by several environmental agencies. Using fly ash either alone or in equal quantity with sewage sludge had little influence on soil properties and heavy metal content. The relative availability (RA) of heavy metals in three soils amended with 52 t ha–1 of sewage sludge was observed to be highest in oxisol, followed by alfisol and vertisol. [ABSTRACT FROM AUTHOR]

Heavy right-handed neutrinos are of current interest. The interactions and decay of such neutrinos determine their decoupling epoch during the evolution of the universe. This in turn affects various observable features like the energy density, nucleosynthesis, CMBR spectrum, galaxy formation and baryogenesis. Here, we consider reduction of right-handed electron-type Majorana neutrinos, in the left–right symmetric model, by the $W_{\mathrm{R}}^+W_{\mathrm{R}}^-$ channel and the channel originating from an anomaly, involving the $SU(2)_{\mathrm{R}}$ gauge group, as well as decay of such neutrinos. We study the reduction of these neutrinos for different ranges of left–right model parameters, and find that, if the neutrino mass exceeds the right-handed gauge boson mass, then the neutrinos never decouple for realistic values of the parameters, but, rather, decay in equilibrium. Because there is no out-of-equilibrium decay, no mass bounds can be set for the neutrinos. [ABSTRACT FROM AUTHOR]

Copyright of Zeitschrift für Physik is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Zeitschrift für Physik is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Zeitschrift für Physik is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)