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Hormesis or hormetic effect is characterized by a biphasic response caused by a stressor or stressor agent. The first phase of hormesis is stimulation at low or sublethal doses, whereas the second phase occurs through inhibition or death at high or lethal doses of the stressor. Both phases are represented using mathematical models in the form of dose-response curves. In plants, hormesis has morphological, physiological, biochemical and molecular effects. These effects act as response mechanisms of adjustment or coupling to the stressor. Adjustment or coupling mechanisms can be exploited in plant tissue culture (PTC) to increase or promote plant length, number of leaves, number of roots, biomass and secondary metabolites, or to induce or enhance morphogenesis in vitro. This review compiles recent findings on hormesis using PTC techniques and the mechanisms of response to different stressors. Stressor agents that present a hormetic dose response (stimulation, inhibition) include silver nanoparticles (50, 100 mg/L), arsenic (1.15, 50.15 mg/kg), multi-walled carbon nanotubes (50, 200 mg/L), 60Co gamma rays (20, 60 Gy), microplastics (0.01, 0.1 mg/L), and a magnetic field (65, 172 mT), among others. In addition, topics such as general plant hormetic response to abiotic stressors, physiological and biochemical aspects of hormesis, hormetic response to metals and nanomaterials, and other curious examples of hormesis are included. Hormesis could be considered as a novel strategy to improve growth and differentiation in different PTC areas and applications. It is suggested to document new cases of hormesis and carry out future studies to elucidate and understand the hormetic effect response mechanisms to different stressors through in vitro biological models. [ABSTRACT FROM AUTHOR]

Purpose of Review: The aim of this review, is to present an updated revision of topical management of SAC and PAC, based on the available scientific evidence and focused on the impact of ophthalmic solution formulations on eye surface. Recent Findings: Physicians treating ocular allergy should be aware of tear film and tear film disruption in SAC and PAC, and how eye drop composition and additives affect the physiology of the allergic eye. Summary: Seasonal and perennial allergic conjunctivitis (SAC and PAC) are the most frequent causes of ocular allergy (OA), and both conditions are underdiagnosed and undertreated. SAC and PAC are immunoglobulin E (IgE)-mediated hypersensitivity reactions. The additional tear film disruption caused by the release of inflammatory mediators increases and exacerbates the impact of signs and symptoms and may trigger damage of the ocular surface. Comorbidities are frequent, and dry eye disease in particular must be considered. Clinical guidelines for the management of SAC and PAC recommend topical therapy with antihistamines, mast cells stabilizers or dualaction agents as first-line treatment, but care should be taken, as many medications contain other compounds that may contribute to ocular surface damage. [ABSTRACT FROM AUTHOR]

Sonchus oleraceus L. (Asteraceae) is a cosmopolitan species native to Europe commonly known as lettuce, sowthistle, chicory, or fake dandelion, considered a weed. However, for many years in various cultures around the world, it has been used as food and medicinal plant. The aim of this integrative review is to document the ethnomedical, phytochemical, and pharmacological information of this species. Forty-one papers document the use of S. oleraceus to heal of a wide variety of diseases. However, gastrointestinal problems, diabetes, inflammation, infections, hepatitis, wounds, and to consume it as food are the most common uses. On the other hand, only 11 items highlight that the main groups of secondary metabolites in this species are flavonoids and terpene lactones. Finally, 45 items reveal that antioxidant, antimicrobial, antiproliferative and cytotoxic were the most studied pharmacological activities. In vitro and in vivo studies of extracts and components isolated from different parts of S. oleraceus have provided a concrete overview of the pharmacological properties of this species that supports its ethnomedical uses in cultures from different parts of the world. The reports of this species have focused solely on the study of the complete plant, leaves, and aerial parts, so it is necessary to study other parts of this species to search for bioactive compounds. No clinical studies were found, which creates an opportunity to expand scientific knowledge of this species. [ABSTRACT FROM AUTHOR]

Multidisciplinary information such as geophysical, hydraulic, geological, and hydrogeological information combined with GIS can be a powerful tool to better understand aquifers. The aim of this paper is to assess groundwater pollution risk through regional hydrogeological and local hydrogeophysical models built from multidisciplinary information and geophysical well logs. Once the regional model of the Toluca Valley Aquifer (Mexico) was built, the critical area (CA) of the aquifer was identified through features such as fractures and faults, pollution sources, subsidence, groundwater table depletion, and well density, which were transformed into GIS with multicriteria analysis. With the CA a local hydrogeophysical model was set to extract lithological information and compute the intrinsic vulnerability (IV) through the Dar Zarrouk parameters according to the AVI-Fr method which considers the influence of fractures and faults as preferential paths for pollutants. On the other hand, the potential harmful load (PHL) of the different pollution sources was estimated with the POSH method. Finally, with the relation between the IV and PHL, risk pollution map could be made. The results of this study show that different areas are exposed to pollution risk due to their proximity to fractures, oil and gas subsurface infrastructure and high pumping ratios and their relationships. The available geophysical data and multidisciplinary information were powerful tools for identifying the critical area of the TVA and proposing actions to mitigate or control excessive withdrawals for sustainable management. [ABSTRACT FROM AUTHOR]

This study aimed to determine the photomixotrophic and physiological responses using different temporary immersion systems (TIS) during in vitro multiplication of agave Tobalá. The culture systems were SETIS™ bioreactor, Temporary Immersion Bioreactor (TIB), Monobloc Advance Temporary Immersion System, and semisolid culture medium as the control. At six weeks of culture, different physiological variables were evaluated: chlorophyll content, stomatal index, percentage of closed stomata, Phosphoenolpyruvate (PEP) and Rubisco during the multiplication stage, and survival percentage in the acclimatization stage. TIS increased multiplication rate (41%), stomatal index (44%), percentage of closed stomata (11%) and chlorophyll content (45%) with respect to the semisolid culture medium system. The highest PEP content (> 35%) was observed in temporary immersion (TI), whereas, Rubisco content, showed no differences among the culture systems evaluated. Regarding survival percentage during acclimatization, the highest shoot survival was obtained in TI, and all regenerate shoots were rooted ex vitro. This study shows that in vitro photomixotrophism was induced in TIS during the multiplication stage. In conclusion, SETIS™ bioreactor and TIB systems are an alternative for mass multiplication of A. potatorum; however, all TIS evaluated guarantee a high survival rate during acclimatization. [ABSTRACT FROM AUTHOR]

Rock fragment size is a key variable in several mining stages such as underground mine design, equipment selection, and mineral processing. In Block Caving, rock fragment sizes are affected by fragmentation during gravity flow in the ore column while ore is being extracted from drawpoints. Additionally, smaller fragments can percolate between large fragments during gravity flow. These two phenomena — rock fragmentation and particle percolation — are not easy to simulate at a large scale in Block Caving. In this paper, a fragmentation model in a cellular automata gravity flow simulator is proposed to model rock fragmentation during flow at large scales. The fragmentation model uses the rock strength, vertical stresses, and travel distance as inputs to estimate the rock breakage and was calibrated with experimental and mine data. The mine scale results show an error of 9% and 7% of the fragmentation in the zones evaluated. This error rate is considered low due to the variability of the phenomena involved. Then, integrating a fragmentation model into a gravity flow simulator can more realistically represent ore fragmentation in caving-mine to generate flow simulations. [ABSTRACT FROM AUTHOR]

This study's aim is to adapt the theory of technostress creators and inhibitors (Ragu-Nathan et al. 2008; Inf Sys Res 19(4):417–433. https://doi.org/10.1287/isre.1070.0165) culturally and linguistically to the French context and evaluate it from the perspective of occupational psychology and ergonomics. This research was divided into two parts. The first included the development of a French version of the scale using the backtranslation method, an assessment of content validity on the advice of expert judges in ergonomics and occupational psychology, and a study of the clarity of the items after the application of this preliminary version to a population similar to the target population. The second part of this research included the psychometric analysis of the French version of the scale after its application to a sample of French workers (n = 307). This process included an evaluation of its internal consistency, an evaluation of its internal structure using a confirmatory factor analysis and of its construct validity using one of the dimensions of the mental workload scale, i.e., available resources (Galy 2020; uant Methods Psychol 16(3):240–252. https://doi.org/10.20982/tqmp.16.3.p240). The results indicate an optimal level of internal consistency of the dimensions, except for dimensions of Techno-Invasion and Techno-Insecurity. Likewise, the results of the confirmatory factor analysis led to the suppression of two measurement items within these same dimensions. Finally, the study confirmed that technostress creators negatively influence the operator's resources while technostress inhibitors positively influence them. [ABSTRACT FROM AUTHOR]

Background: Biochemical markers and imaging tests have been used with the aim of stratifying the risk and detecting atrial fibrosis. Speckle-tracking echocardiography (STE) is used for the detection of atrial fibrosis and Gal-3 provides an important prognostic value. The objective of the study was to assess the association between atrial fibrosis markers and serum levels, genetic polymorphisms and genic expression of galectin-3. Methods: 206 patients with permanent AF and 70 patients with paroxysmal AF were included in the study. Real time PCR (TaqMan) system was used to study SNPs rs4652 and 4644 of the gene LGALS3. Serum levels of Gal-3 were determined by ELISA and STE was performed to assess fibrosis. Results: Mean age of individuals with permanent AF was 66.56 ± 12 years. As for the echocardiography results, those patients showed an decrease in the following parameters peak atrial longitudinal strain (PALS) (p = 0.002) when compared to the same parameters from the paroxysmal AF group of patients. There was a correlation between serum levels of Gal-3 and PALS in the group of patients with permanent AF; the lower the levels of gal-3, the lower the LA strain (r = 0.24; p = 0.01). Conclusions: Echocardiographic findings showed association with the groups, and with serum levels of Gal-3 in patients with permanent AF. The distribution of allelic and genotypic frequencies, and of the haplotypes of polymorphism LGALS3 rs4652 and rs4644 did not present statistical variation, which suggests that those SNPs are not associated with the AF clinical forms (permanent and paroxysmal). [ABSTRACT FROM AUTHOR]

The term in vitro photomixotrophism refers to the ability of explants to obtain metabolic energy from the culture medium and as a product of photosynthesis. The objective of this research was to study the physiological and biochemical mechanisms of V. planifolia during in vitro multiplication using a photomixotrophic system with different sucrose contents (0, 15 and 30 g L–1) and CO2 supply levels (500, 800 and 1200 ppm) using a Temporary Immersion Modular System (SMIT®). After 45 days of multiplication, response percentage, number of shoots per explant, shoot length, number of leaves per shoot, stomatal index (%), percentage of closed stomata, and chlorophyll, β-carotene, Phosphoenolpyruvate (PEP) and Rubisco contents were evaluated. In addition, the survival rate during acclimatization at 60 days was evaluated. For the multiplication stage, the highest response percentage was obtained in the treatments with 15 g L–1 sucrose with 500 and 800 ppm CO2, and 30 g L–1 sucrose with 500 ppm CO2. In the latter treatment, the best development parameters were obtained, with 14.75 shoots per explant, a shoot length of 2.38 cm and 2.5 leaves per shoot. In general, the highest chlorophyll, β-carotene, PEP and Rubisco contents were observed with 30 g L–1 sucrose + CO2. No effects of the treatments on stomatal index (%) were observed, while the percentage of closed stomata showed differences among treatments. At the acclimatization stage, the highest survival percentages were obtained from the treatments of 30 g L–1 sucrose with 500 and 800 ppm CO2. In conclusion, this study demonstrates physiological and biochemical mechanisms for a better understanding of photomixotrophism during in vitro multiplication in vanilla and may be applied to other species. Key message: In vitro photomixotrophism can be induced by injecting carbon dioxide to promote photosynthesis. [ABSTRACT FROM AUTHOR]

Mimetic finite difference operators D , G are discrete analogs of the continuous divergence (div) and gradient (grad) operators. In the discrete sense, these discrete operators satisfy the same properties as those of their continuum counterparts. In particular, they satisfy a discrete extended Gauss' divergence theorem. This paper investigates the higher-order quadratures associated with the fourth- and sixth- order mimetic finite difference operators, and show that they are indeed numerical quadratures and satisfy the divergence theorem. In addition, extensions to curvilinear coordinates are treated. Examples in one and two dimensions to illustrate numerical results are presented that confirm the validity of the theoretical findings. [ABSTRACT FROM AUTHOR]

Objective: To examine the relationship between periodontitis and subclinical intracranial atherosclerosis. The association of periodontitis with preclinical markers of atherosclerosis in other vascular territories was also explored. Material and methods: This was a cross-sectional study where 97 elderly subjects with a previous history of hypertension received an ultrasonographic evaluation to assess subclinical atherosclerosis in different vascular territories: (1) cerebral [pulsatility (PI) and resistance index (RI) of the middle cerebral artery], (2) carotid [intima-media thickness (IMT)], and (3) peripheral [ankle-brachial index (ABI)]. Additionally, participants underwent a full-mouth periodontal assessment together with blood sample collection to determine levels of inflammatory biomarkers (leukocytes, fibrinogen, and erythrocyte sedimentation rate), lipid fractions (total cholesterol and high- and low-density lipoprotein), and glucose. Results: Sixty-one individuals had periodontitis. Compared to subjects without periodontitis, those with periodontitis showed higher values of PI (1.24 ± 0.29 vs 1.01 ± 0.16), RI (0.70 ± 0.14 vs 0.60 ± 0.06), and IMT (0.94 ± 0.15 vs 0.79 ± 0.15) (all p < 0.001). No statistically significant differences were found neither for ABI or for other clinical and biochemical parameters. An independent association was found between periodontitis and increased intracranial atherosclerosis (ORadjusted = 10.16; 95% CI: 3.14–32.90, p < 0.001) and to a lesser extent with thicker carotid IMT (ORadjusted = 4.10; 95% CI: 1.61–10.48, p = 0.003). Conclusions: Periodontitis is associated with subclinical atherosclerosis in both intracranial and carotid arteries in elderly subjects with hypertension. Clinical relevance: The association of periodontitis with intracranial atherosclerosis implies that periodontitis patients might have greater chances to develop ischemic stroke in the future. [ABSTRACT FROM AUTHOR]

Ralstonia solanacearum species complex (RSSC) is the causal agent of bacterial wilt disease in many plant species, including important crops. It is composed of a heterogeneous group of strains whose phylogeny indicates four main groups (phylotypes) which have been re-arranged into three different species: R. pseudosolanacearum, R. solanacearum, and R. syzygii. Strains belonging to the four phylotypes were isolated from different continents indicating that geographic isolation and spatial distance have structured the populations and shaped the evolving process to become different species (allopatric speciation). RSSC strains can also live in soil and the surrounding water. Soil bacteria adapt to new environmental conditions through genome changes which are facilitated by the acquisition of genetic material through horizontal gene transfer. These genetic changes and ecological fit may drive bacterial evolution following sympatric speciation. Here, comparative genomic tools were used to investigate the speciation process of RSSC by studying the essential and flexible gene content and the openness of the pangenome of 314 genomic sequences. The results indicate that the vast majority of genes in RSSC pangenome are dispensable with only 228 essential genes. The pangenome of RSSC is open, which is characteristic of sympatric species. The R. pseudosolanacearum, R. solanacearum, and R. syzygii pangenomes are also open, however, the pangenome of R. syzygii is near to turn closed. Phylogenetic and statistical evidence reported in the literature and the results obtained in this work suggest that both allopatric and sympatric lifestyles have shaped the speciation process of RSSC. [ABSTRACT FROM AUTHOR]

In the last years, considerable research has been carried out to develop robots that can improve our quality of life during tedious and challenging tasks. In these contexts, robots operating without human supervision open many possibilities to assist people in their daily activities. When autonomous robots collaborate with humans, social skills are necessary for adequate communication and cooperation. Considering these facts, endowing autonomous and social robots with decision-making and control models is critical for appropriately fulfiling their initial goals. This manuscript presents a systematic review of the evolution of decision-making systems and control architectures for autonomous and social robots in the last three decades. These architectures have been incorporating new methods based on biologically inspired models and Machine Learning to enhance these systems' possibilities to developed societies. The review explores the most novel advances in each application area, comparing their most essential features. Additionally, we describe the current challenges of software architecture devoted to action selection, an analysis not provided in similar reviews of behavioural models for autonomous and social robots. Finally, we present the future directions that these systems can take in the future. [ABSTRACT FROM AUTHOR]

Adapting to dynamic environments is essential for artificial agents, especially those aiming to communicate with people interactively. In this context, a social robot that adapts its behaviour to different users and proactively suggests their favourite activities may produce a more successful interaction. In this work, we describe how the autonomous decision-making system embedded in our social robot Mini can produce a personalised interactive communication experience by considering the preferences of the user the robot interacts with. We compared the performance of Top Label as Class and Ranking by Pairwise Comparison, two promising algorithms in the area, to find the one that best predicts the user preferences. Although both algorithms provide robust results in preference prediction, we decided to integrate Ranking by Pairwise Comparison since it provides better estimations. The method proposed in this contribution allows the autonomous decision-making system of the robot to work on different modes, balancing activity exploration with the selection of the favourite entertaining activities. The operation of the preference learning system is shown in three real case studies where the decision-making system works differently depending on the user the robot is facing. Then, we conducted a human–robot interaction experiment to investigate whether the robot users perceive the personalised selection of activities more appropriate than selecting the activities at random. The results show how the study participants found the personalised activity selection more appropriate, improving their likeability towards the robot and how intelligent they perceive the system. query Please check the edit made in the article title. [ABSTRACT FROM AUTHOR]

We investigated microcrustaceans inhabiting arsenic contaminated and non-contaminated freshwater to identify potential bioindicators of arsenic contamination in the tropical freshwater of Matehuala in northern Mexico. We collected water, sediment, and zooplankton, at five sampling points during three sampling campaigns. We determined water temperature, pH, electrical conductivity, dissolved oxygen, alkalinity, salinity, and total arsenic concentration in water. Additionally, we determined total arsenic and arsenic speciation in sediment samples. We identified microcrustaceans and determined abundance, richness, and Shannon Index. We also investigated relationships and correlations between physiochemical and ecological variables. Results showed that arsenic concentrations in freshwater ranged from 0.001 to 53.23 mg/L, while total arsenic in sediments ranged from 10.37 to 2472.84 mg/kg as As + 5. Six microcrustacean species were found in highly and moderately contaminated water (Latonopsis australis, Eucyclops chihuahuensis, Acanthocyclops americanus, Pleuroxus (Picripleuroxus) quasidenticulatus, Macrocyclops albidus, and Paracyclops chiltoni), while five species were found in arsenic-free water (Simocephalus punctatus, Alona glabra, Eucyclops leptacanthus, M. albidus, and P. quasidenticulatus). An inverse relationship was observed between microcrustacean richness and arsenic. However, the scope of the data did not allow for a strong and significant correlation. Nevertheless, among the species inhabiting As-free water, S. punctatus showed potential to be further tested as a bioindicator of As contamination in Matehuala. Identification of potential bioindicators could help monitor water quality and increase understanding of the incorporation and toxicity of As in freshwater-sensitive and freshwater-metallotolerant microcrustaceans, which, in turn, might help us to understand As incorporation in the food web. [ABSTRACT FROM AUTHOR]

Description and morphological analysis of copepods inhabiting a water body with high arsenic concentrations (32.79 to 62.29 mg L−1) were performed to identify some effect on the development of individuals due to the arsenic concentrations. Detailed morphology of prosomal and urosomal appendages along the development of the specimens was considered. The results showed that the freshwater copepod Paracyclops novenarius Reid, 1987 inhabits this water body, and previously, it was recorded as Paracyclops chiltoni (Thomson GM, 1882) on this site. Moreover, this becomes the first record of P. novenarius in Mexico. Morphological analysis showed a normal and stable development along the different instars, different arsenic concentrations in the media, and different sampled dates between the analyzed specimens, suggesting that the high arsenic concentrations do not affect the morphology of P. novenarius, including all its development and adult instars, which differs from other copepods and other groups such as Cladocera and Rotifera, where morphological changes due to metals and metalloids have been observed but in low concentrations of these elements. The results of this study contribute to the existing reports of the genus Paracyclops (Claus 1893) in Mexico and could provide information for environmental impact assessments on aquatic systems. [ABSTRACT FROM AUTHOR]

Medium-term in vitro plant conservation is an alternative for maintaining germplasm banks in breeding programs. The aim of this study was to evaluate the efficiency of the gibberellin inhibitors paclobutrazol (PBZ) and trinexapac-ethyl (TNE) for in vitro conservation by minimal growth in anthurium, sugarcane, and agave. For the establishment of the conservation system, shoots of the aforementioned species were taken at different concentrations (0, 1, 2, and 3 mg L−1) of PBZ and TNE. At 180 d of culture, the survival percentage, number of shoots, plant length, number of leaves, number of roots, root length, total chlorophyll content, stomatal density, and survival percentage during acclimatization were evaluated. According to the variables evaluated, the results showed that in anthurium 1 mg L−1 PBZ was the best treatment for in vitro conservation without affecting survival, while in sugarcane it was 1 mg L−1 PBZ and agave 3 mg L−1 PBZ or TNE. Survival during acclimatization was 97, 96, and 98% in anthurium, sugarcane, and agave, respectively. In this study, the gibberellin-inhibiting compounds PBZ and TNE are a low-cost alternative for in vitro conservation of anthurium, sugarcane, and agave and can be evaluated in other species. [ABSTRACT FROM AUTHOR]

The National Institutes of Health Stroke Scale (NIHSS) is commonly used to evaluate stroke neurological deficits and to predict the patient's outcome. Neurological instability (NI), defined as the variation of the NIHSS in the first 48 h, is a simple clinical metric that reflects dynamic changes in the area of the brain affected by the ischemia. We hypothesize that NI may represent areas of cerebral instability known as penumbra, which could expand or reduce brain injury and its associated neurological sequels. In this work, our aim was to analyze the association of NI with the functional outcome at 3 months and to study clinical biomarkers associated to NI as surrogate biomarkers of ischemic and inflammatory penumbrae in ischemic stroke (IS) patients. We included 663 IS patients in a retrospective observational study. Neutral NI was defined as a variation in the NI scale between − 5 and 5% (37.1%). Positive NI is attributed to patients with an improvement of > 5% NI after 48 h (48.9%), while negative NI is assigned to patients values lower than − 5% (14.0%). Poor outcome was assigned to patients with mRS ≥ 3 at 3 months. We observed an inverse association of poor outcome with positive NI (OR, 0.35; 95%CI, 0.18–0.67; p = 0.002) and a direct association with negative NI (OR, 6.30; 95%CI, 2.12–18.65; p = 0.001). Negative NI showed a higher association with poor outcome than most clinical markers. Regarding good functional outcome, positive NI was the marker with the higher association (19.31; CI 95%, 9.03–41.28; p < 0.0001) and with the highest percentage of identified patients with good functional outcome (17.6%). Patients with negative NI have higher glutamate levels compared with patients with neutral and positive NI (p < 0.0001). IL6 levels are significantly lower in patients with positive NI compared with neutral NI (p < 0.0001), while patients with negative NI showed the highest IL6 values (p < 0.0001). High glutamate levels were associated with negative NI at short latency times, decreasing at higher latency times. An opposite trend was observed for inflammation, and IL6 levels were similar in patients with positive and negative NI in the first 6 h and then higher in patients with negative NI. These results support NI as a prognosis factor in IS and the hypothesis of the existence of a delayed inflammatory penumbra, opening up the possibility of extending the therapeutic window for IS. [ABSTRACT FROM AUTHOR]

Ralstonia solanacearum species complex (RSSC) is a bacterial group of aggressive and destructive plant pathogens. RSSC strains show a broad host range and adaptation to different environments. The key machinery for RSSC virulence is the Type III secretion system, which enables the secretion and injection of specialized proteins (Type III-secreted effectors or T3Es) into plant cells. T3Es interact with host plant proteins altering the normal metabolism of plant cells and reducing the plant defences. Recognition of protein–protein interactions (PPIs) is fundamental to understanding the pathogenic process and adaptation to different hosts and environmental conditions. Here, we used an in silico approach to describe PPIs between two system models, T3Es of R. pseudosolanacearum GMI1000 and Solanum lycopersicum proteins and T3Es of R. solanacearum UY031 and Solanum tuberosum proteins. Matching results of two independent analysis methods (interolog and domain-based method) employed to detect PPIs were taken as verified interactions. Results show that 12,261 PPIs are possible involving 11 T3Es. A comparison analysis of PPIs between the two system models allowed us to find some T3Es homologs which are differentially interacting with plant proteins and may explain hosts specificity and environmental conditions of RSSC. These T3Es interact with a wide variety of plant proteins that play a central role in immune responses, intracellular signalling, and metabolic processes. [ABSTRACT FROM AUTHOR]

Drought-induced water stress affects the productivity of the Vanilla planifolia Jacks. ex Andrews crop. In vitro culture technique is an effective tool for the study of water stress tolerance mechanisms. This study aimed to evaluate the morphological, physiological and biochemical response of V. planifolia under in vitro water stress conditions induced with polyethylene glycol (PEG). In vitro regenerated shoots of 2 cm in length were subjected to different concentrations of PEG 6000 (0, 1, 2 and 3% w/v) using Murashige and Skoog semi-solid culture medium. At 60 days of culture, different growth variables, dry matter (DM) content, chlorophyll (Chl), soluble proteins (SP), proline (Pro), glycine betaine (GB), stomatal index (SI) and open stomata (%) were evaluated. Results showed a reduction in growth, Chl content, SP, SI and open stomata (%) with increasing PEG concentration, whereas DM, Pro and GB contents rose with increasing PEG concentration. In conclusion, PEG-induced osmotic stress allowed describing physiological and biochemical mechanisms of response to water stress. Furthermore, the determination of compatible Pro and GB osmolytes can be used as biochemical markers in future breeding programs for the early selection of water stress tolerant genotypes. [ABSTRACT FROM AUTHOR]

Ruminants are capable of transforming biomass into useful products; therefore, they can be considered as consolidated processing units. A conceptual design is presented for a biorefinery scheme, imitating a ruminant, under a consolidated bioprocessing (CBP) approach for ABE (acetone-butanol-ethanol) fermentation. The biorefinery was simulated by using SuperPro Designer® v8.5 software. Wheat straw (WS) was used as feedstock. A sensitivity analysis of the impact of the butanol output concentration of the ABE fermentation reactor on butanol total production cost (TPC) was made. Butanol concentrations (9, 15, and 19 g/L) and a hydraulic retention time (HRT) of 72 h were fixed on previous studies using specific species of Clostridium. Using the best scenario, a sensitive analysis of TPC was explored for different feedstock capacities of 400, 600, 800, 1000, 1400, 1800, and 2200 MT DB/day. The results showed a TPC of US$0.98/kg for the best-case scenario (2200 MT of WS/day; 19 g of butanol per L; HRT of 72 h), which is competitive with current gasoline prices in Mexico. The biorefinery produced all of its steam and electricity requirements by means of the co-production of the hydrogen and methane that were used in the co-generation stage, along with lignin. In addition, the production of butanol against electricity and its impact on the economy of the process was compared. Finally, by increasing the production of methane for the production of electricity, the economic parameters were negatively affected for all scenarios. [ABSTRACT FROM AUTHOR]

Scaling-up techniques in temporary immersion systems are an alternative for commercial micropropagation. In vitro propagation of pitahaya (Hylocereus undatus) using temporary immersion with liquid culture medium improves micropropagation efficiency compared to the conventional method in semisolid medium. The objective of this work was to evaluate the efficiency of traditional culture and temporary immersion during micropropagation of pitahaya to facilitate the rapid establishment of new commercial plantations of high genetic and phytosanitary quality. Semisolid culture, liquid media in partial immersion and temporary immersion in an Ebb-and-Flow bioreactor were evaluated. Also, in temporary immersion, different immersion frequencies (every 4, 8, 12, and 16 h) and culture densities (5, 10, 15 and 20 explants per bioreactor) were evaluated. For the multiplication stage, new shoots and length were recorded per explant at 45 d of in vitro culture and in the acclimatization stage, the survival percentage was determined at 30 d of greenhouse cultivation. A temporary immersion of 2 min every 4 h and 15 explants per bioreactor was the best culture system, obtaining on average 10.7 shoots per explant with a length of 1.9 cm. No significant differences were observed among treatments during acclimatization, obtaining survival percentages of 98%–100%. This study reports for the first time a protocol for scaling-up techniques in temporary immersion for commercial micropropagation of pitahaya (and for any species of the Cactaceae family) and its establishment in a productive plantation. [ABSTRACT FROM AUTHOR]

At the beginning of the Cold War, the United States established military bases at various points around the globe to counter Soviet influence. The construction of these bases was an unprecedented undertaking because its scope. In Spain, an extensive network of military installations was built throughout the country in only 5 years. The main nuclei of this network were the bases of Torrejón, Zaragoza, Morón, and Rota. This article aims to show that these bases share a common design project which implements an underlying grid-like system in the organization of their masterplans. After detailed study of the Torrejón air base, we aim to demonstrate that this grid-like system was conceived as a sophisticated and precise graphic tool able to facilitate the design, construction, and maintenance of the bases, as well as to back an ideological project. [ABSTRACT FROM AUTHOR]

We research into the clinical, biochemical and neuroimaging factors associated with the outcome of stroke patients to generate a predictive model using machine learning techniques for prediction of mortality and morbidity 3-months after admission. The dataset consisted of patients with ischemic stroke (IS) and non-traumatic intracerebral hemorrhage (ICH) admitted to Stroke Unit of a European Tertiary Hospital prospectively registered. We identified the main variables for machine learning Random Forest (RF), generating a predictive model that can estimate patient mortality/morbidity according to the following groups: (1) IS + ICH, (2) IS, and (3) ICH. A total of 6022 patients were included: 4922 (mean age 71.9 ± 13.8 years) with IS and 1100 (mean age 73.3 ± 13.1 years) with ICH. NIHSS at 24, 48 h and axillary temperature at admission were the most important variables to consider for evolution of patients at 3-months. IS + ICH group was the most stable for mortality prediction [0.904 ± 0.025 of area under the receiver operating characteristics curve (AUC)]. IS group presented similar results, although variability between experiments was slightly higher (0.909 ± 0.032 of AUC). ICH group was the one in which RF had more problems to make adequate predictions (0.9837 vs. 0.7104 of AUC). There were no major differences between IS and IS + ICH groups according to morbidity prediction (0.738 and 0.755 of AUC) but, after checking normality with a Shapiro Wilk test with the null hypothesis that the data follow a normal distribution, it was rejected with W = 0.93546 (p-value < 2.2e−16). Conditions required for a parametric test do not hold, and we performed a paired Wilcoxon Test assuming the null hypothesis that all the groups have the same performance. The null hypothesis was rejected with a value < 2.2e−16, so there are statistical differences between IS and ICH groups. In conclusion, machine learning algorithms RF can be effectively used in stroke patients for long-term outcome prediction of mortality and morbidity. [ABSTRACT FROM AUTHOR]

Delineating spring protection zones is key to managing groundwater. This work presents a proposal for delineating spring protection zones (SPZs) that uses hydrogeological, topographical, land use, and climate characteristics as a basis to protect springs located in fractured volcanic media from potential contamination processes. This was accomplished through five stages: (1) identification of hydrogeological characteristics of the environments in which springs are located and physicochemical properties of water, (2) delineation of spring potential catchment zones, (3) estimation of spring recharge zones in the potential catchment zones, (4) SPZ proposal based on annual recharge analysis for each spring, and (5) projection of future land use and climate change scenarios. The result was a proposal of three typically established zones for protecting springs: SPZ1 was defined by a 50-m radius around springs, SPZ2 was delineated based on spring annual recharge zone estimate, and SPZ3 was considered the remainder of potential catchment zone. By delineating these zones, more suitable protection measures can be identified based on trends in land use and climate changes, measures which would thereby aid in sustainable use of these types of springs. [ABSTRACT FROM AUTHOR]

This work was designed to analyze the current ecological niche of Peridinium quadridentatum var. quadridentatum and its harmful algal blooms (HABs) using species distribution models. A maximum entropy model was fitted to samples of occurrence records gathered from the scientific literature and using environmental data for the continental shelves of the world obtained from BIO-ORACLE. The geographic models plotted were sea surface temperature vs. salinity, nitrate vs. phosphate concentration, and radiation vs. chlorophyll-a concentration, to describe the environmental space occupied by P. quadridentatum. Our results show that P. quadridentatum is a dinoflagellate of wide thermohaline tolerance linked to sites near coastal areas, which might be related to some life-cycle stages. Both presence (pENM) and blooms (bENM) ecological niche models show that this species prospers near tropical and temperate latitudes. The pENM predicts a broader distribution range than the bENM, suggesting that some sites with favorable conditions for the occurrence of this species are not suitable for its proliferation and formation of HABs. The bENM predicts potential HABs limited in eutrophic areas, but not in hyper-eutrophic areas. As validation of the models, some occurrence records of this species (i.e., West Africa, Peru, and Fiji) were not included in the initial analyses. As a result, the pENM predicts its occurrence in those sites, so the models for current potential distribution and blooms incidences are credible. [ABSTRACT FROM AUTHOR]

Most observed institutional arrangements, in governments, firms, and other organizations, acknowledge the effectiveness of imposing sanctioning institutions and monitoring policies to achieve particular goals. However, less attention has been paid to the influences of the delegation mechanism of sanctioning power. In particular, it remains unclear whether the mechanism influences the legitimacy of the authority/institution, in centralized institutional arrangements. We report laboratory-experimental results of a public goods game that compare the performance of exogenous (i.e., the Leviathan) versus endogenous (i.e., the Democracy) delegation of sanctioning power. Observed differences are not statistically significant, regardless of the effectiveness of sanctions imposed, tested in two experiments with different punishment/cost functions. Democratic schemes in centralized power environments should not be taken for granted. Experimental evidence contradicts the common belief of a robust causal relationship between indirect democratic institutions, collective action, and economic outcomes. [ABSTRACT FROM AUTHOR]

The unceasing aging of the population is leading to new problems in developed countries. Robots represent an opportunity to extend the period of independent living of the elderly as well as to ameliorate their economic burden and social problems. We present a new social robot, Mini, specifically designed to assist and accompany the elderly in their daily life either at home or in a nursing facility. Based on the results of several meetings with experts in this field, we have built a robot able to provide services in the areas of safety, entertainment, personal assistance and stimulation. Mini supports elders and caregivers in cognitive and mental tasks. We present the robot platform and describe the software architecture, particularly focussing on the human–robot interaction. We give in detail how the robot operates and the interrelation of the different modules of the robot in a real use case. In the last part of the paper, we evaluated how users perceive the robot. Participants reported interesting results in terms of usability, appearance, and satisfaction. This paper describes all aspects of the design and development of a new social robot that can be used by other researchers who face the multiple challenges of creating a new robotic platform for older people. [ABSTRACT FROM AUTHOR]

The main objective of this research work was to study the association of serum levels of growth factors (GF) and SDF-1α with the functional outcome and reduction of lesion volume in ischemic stroke patients. In this multicenter study, 552 patients with non-lacunar stroke (male, 62.1%; mean age, 68.2 ± 11.4) were included within 24 h from symptom onset. The main outcome variable was good functional outcome (modified Rankin Scale [mRS] ≤ 2) at 12 months. Secondary outcome variable was infarct volume (in mL) after 6 ± 3 months. Serum levels of VEGF, Ang-1, G-CSF, BDNF, and SDF-1α were measured by ELISA at admission, 7 ± 1 days, at 3 ± 1 months, and 12 ± 3 months. Except for BDNF, all GF and SDF-1α serum levels showed a peak value at day 7 and remained elevated during the first 3 months (all p < 0.01). High serum levels at day 7 of VEGF (OR, 19.3), Ang-1 (OR, 14.7), G-CSF (OR, 9.6), and SDF-1α (OR, 28.5) were independently associated with good outcome at 12 months (all p < 0.0001). On the other hand, serum levels of VEGF (B, − 21.4), G-CSF (B, − 14.0), Ang-1 (B, − 13.3), and SDF-1α (B, − 44.6) measured at day 7 were independently associated with lesion volume at 6 months (p < 0.01). In summary, high serum levels of VEGF, Ang-1, G-CSF, and SDF-1α at day 7 and 3 months after ischemic stroke are associated with good functional outcome and smaller residual lesion at 1 year of follow-up. [ABSTRACT FROM AUTHOR]

The emergence of information and communication technologies (ICT) has generated a number of research questions, related to their use and potential risk, but also potentials for prevention or health promotion. Online social networks have become an important source of information for users as well as a tool for social relations. As traditional social networks, they can act as vehicles to improve the health of adolescents and youth, as well as play a key role in an educational context. The aim of this work is then to explore the theoretical relevance of ICT, particularly on online social networks, on disease prevention and health promotion of communicable diseases. Literature review points out the role of online social networks, particularly in the field of sexual health, body image, especially eating habits and overweight, as well as smoking and alcohol dependence. Data allow us to understand how online social network behavior and interaction is related to their burden and interventions developed in sexual health and addiction show positive results. More efforts in body image are needed in order to use these tools for prevention and promotion of health from early age. [ABSTRACT FROM AUTHOR]

The objective of this preclinical in vivo study was to determine changes in vascular inflammatory biomarkers in systemic circulation after injection of lipopolysaccharide (LPS) from Porphyromonas gingivalis (Pg) in rats. Experimental periodontitis was induced by injections of Pg-LPS. Gingival soft and hard tissues changes were analysed by means of magnetic resonance imaging and micro computed tomography. Serum levels of interleukin (IL)-6, IL-10, pentraxin (PTX) 3, and soluble fragment of tumor necrosis factor-like weak inducer of apoptosis (sTWEAK) were determined at baseline and 24 h, 7, 14, and 21 days after periodontal induction. Significant periodontal inflammation and alveolar bone loss were evident at the end of periodontal induction. Experimental periodontitis posed an acute systemic inflammatory response with increased serum levels of IL-6 and PTX3 at 24 h post-induction, followed by a significant overexpression of sTWEAK at 7 days. This inflammatory state was maintained until the end of the experiment (21 days). As expected, IL-10 serum levels were significantly lower during the follow-up compared to baseline concentrations. In the present animal model, experimental periodontitis is associated with increased systemic inflammation. Further studies are needed to confirm whether PTX3 and sTWEAK could be useful biomarkers to investigate potential mechanisms underlying the relationship between periodontitis and atherosclerotic vascular diseases. [ABSTRACT FROM AUTHOR]

It has recently emerged the concept of "obesity paradox," a term used to describe the unexpected improved prognosis and lower mortality rates found in several diseases in patients with higher body weight. Concerning stroke, few clinical studies have assessed this obesity paradox showing contradictory results. Therefore, our aim was to compare clinical evolution and inflammatory balance of obese and non-obese patients after ischemic stroke. We designed a prospective case-control study in patients with acute ischemic stroke categorized into obese (body mass index, BMI ≥ 30 kg/m2) and non-obese (BMI < 30 kg/m2). We compared clinical, anthropometric, radiological, and laboratory variables. The main outcome variable was the functional outcome at 3 months. We included 98 patients (48 non-obese and 50 obese). No differences in functional outcome at 3 months were found (p = 0.882) although a tendency of a greater recovery on neurological impairments was seen in obese subjects. Importantly, obese patients (p = 0.007) and patients who experienced poor outcome (p = 0.006) exhibited a higher reduction in body weight at 3 months after stroke. Moreover, pro-inflammatory IL-6 levels (p = 0.002) were higher in the obese group. However, IL-6 levels decreased over the first week in obese while increased in non-obese. On the contrary, levels of the anti-inflammatory IL-10 rose over the first week in obese patients, whereas remained stable in non-obese. In summary, despite exhibiting several factors associated with poor outcome, obese patients do not evolve worse than non-obese after ischemic stroke. Obesity may counterbalance the inflammatory reaction through an anti-inflammatory stream enhanced in the first moments of stroke. [ABSTRACT FROM AUTHOR]

Introduction: Double-lumen endotracheal tubes (DL-ETT) and bronchial blockers (BB) have both been used for lung isolation in video-assisted thoracic surgery (VATS). Though not well studied, it is widely thought that a DL-ETT provides faster and better quality lung collapse. The aim of this study was to compare a BB technique vs a left-sided DL-ETT strategy with regard to the time and quality of lung collapse during one-lung ventilation (OLV) for elective VATS.Methods: Forty patients requiring OLV for VATS were randomized to receive a BB (n = 20) or a left-sided DL-ETT (n = 20). The primary endpoint was the time from pleural opening (performed by the surgeon) until complete lung collapse. The time was evaluated offline by reviewing video recorded during the VATS. The quality of lung deflation was also graded offline using a visual scale (1 = no lung collapse; 2 = partial lung collapse; and 3 = total lung collapse) and was recorded at several time points after pleural incision. The surgeon also graded the time to complete lung collapse and quality of lung deflation during the procedure. The surgeon's guess as to which device was used for lung isolation was also recorded.Results: Of the 40 patients enrolled in the study, 20 patients in the DL-ETT group and 18 in the BB group were analyzed. There mean (standard deviation) time to complete lung collapse of the operative lung was significantly faster using the BB compared with using the DL-ETT [7.5 (3.8) min vs 36.6 (29.1) min, respectively; mean difference, 29.1 min; 95% confidence interval, 1.8 to 7.2; P < 0.001]. Overall, a higher proportion of patients in the BB group than in the DL-ETT group achieved a quality of lung collapse score of 3 at five minutes (57% vs 6%, respectively; P < 0.004), ten minutes (73% vs 14%, respectively; P = 0.005), and 20 min (100% vs 25%, respectively; P = 0.002) after opening the pleura. The surgeon incorrectly guessed the type of device used in 78% of the BB group and 50% of the DL-ETT group (P = 0.10).Conclusion: The time and quality of lung collapse during OLV for VATS was significantly better when using a BB than when using a left-sided DL-ETT. Surgeons could not reliably determine which device was being used based on the time and quality of lung collapse. This trial was registered at ClinicalTrials.gov number, NCT01615263. [ABSTRACT FROM AUTHOR]

Purpose: To describe and analyze the biomicroscopic features and in vivo confocal microscopy of the crystalline form of pre-Descemet corneal dystrophy (PDCD).Methods: We examined two non-related families using biomicroscopy, in vivo confocal microscopy, and a genetic study using a gene panel test, looking for mutations in the PIKFYVE gene.Results: A slit-lamp examination of the first family revealed polychromatic crystalline punctiform opacities distributed all over the stroma in 8 of 11 family members in three generations with an autosomal dominant inheritance. The second family showed in three of four members in two generations the same opacities located in the pre-Descemet region. It was also a hint for autosomal dominant inheritance. The in vivo confocal microscopy identified numerous rounded and hyperreflective stromal particles measuring 10-15 μm in diameter, with the highest density in the posterior stroma and with normal keratocytes. No systemic disease was diagnosed. No variants or mutations were identified in PIKFYVE gene.Conclusions: Polychromatic deposits in patients with Punctiform and Polychromatic Pre-Descemet corneal dystrophy can be located not only in the deep stroma but also in the anterior and middle stroma. Our presentation reveals the possibility of considering this characteristic corneal disorder as a corneal dystrophy of its own and not as a subtype of pre-Descemet corneal dystrophy. [ABSTRACT FROM AUTHOR]

Based on preclinical studies suggesting that recombinant tissue plasminogen activator (rt-PA) may promote ischemic brain injuries, we investigated in patients the possible risk of worse clinical outcome after rt-PA treatment as a result of its inability to resolve cerebral ischemia. Here, we designed a cohort study using a retrospective analysis of patients who received treatment with intravenous (4.5-h window) or intraarterial rt-PA, without or with thrombectomy. Controls were consecutive patients who did not receive recanalization treatment, who met all inclusion criteria. As a marker of reperfusion, we defined the variable of early neurological improvement as the difference between the score of the National Institute of Health Stroke Scale (NIHSS) (at admission and 24 h). The main variable was worsening of the patient’s functional situation in the first 3 months. To compare quantitative variables, we used Student’s t test or the Mann-Whitney test. To estimate the odds ratios of each independent variable in the patient’s worsening in the first 3 months, we used a logistic regression model. We included 1154 patients; 577 received rt-PA, and 577 served as controls. In the group of patients treated with rt-PA, 39.4% who did not present clinical reperfusion data developed worsening within 3 months after stroke compared with 3.5% of patients with reperfusion (P < 0.0001). These differences were not significant in the control group. In summary, administration of rt-PA intravenously or intraarterially without reperfusion within the first 24 h may be associated with a higher risk of functional deterioration in the first 3 months. [ABSTRACT FROM AUTHOR]

It is well known that El Niño-Southern Oscillation (ENSO) modifies precipitation patterns in several parts of the world. One of the most impacted areas is the western coast of South America, where Ecuador is located. El Niño events that occurred in 1982-1983, 1987-1988, 1991-1992, and 1997-1998 produced important positive rainfall anomalies in the coastal zone of Ecuador, bringing considerable damage to livelihoods, agriculture, and infrastructure. Operational climate forecasts in the region provide only seasonal scale (e.g., 3-month averages) information, but during ENSO events it is key for decision-makers to use reliable sub-seasonal scale forecasts, which at the present time are still non-existent in most parts of the world. This study analyzes the potential predictability of coastal Ecuador rainfall at monthly scale. Instead of the discrete approach that considers training models using only particular seasons, continuous (i.e., all available months are used) transfer function models are built using standard ENSO indices to explore rainfall forecast skill along the Ecuadorian coast and Galápagos Islands. The modeling approach considers a large-scale contribution, represented by the role of a sea-surface temperature index, and a local-scale contribution represented here via the use of previous precipitation observed in the same station. The study found that the Niño3 index is the best ENSO predictor of monthly coastal rainfall, with a lagged response varying from 0 months (simultaneous) for Galápagos up to 3 months for the continental locations considered. Model validation indicates that the skill is similar to the one obtained using principal component regression models for the same kind of experiments. It is suggested that the proposed approach could provide skillful rainfall forecasts at monthly scale for up to a few months in advance. [ABSTRACT FROM AUTHOR]

Smart people detection systems are nowadays using heterogeneous cameras. This paper proposes an architecture which is focused on robustly detecting people by infrared and visible video fusion in smart environment. The architecture covers all levels provided by the INT $$^3$$ -Horus framework, initially designed to perform monitoring and activity interpretation tasks. Indeed, INT $$^3$$ -Horus is used as the development environment where the approach starts with image segmentation in both infrared and visible spectra. Then, the results are fused to enhance the overall detection performance. The paper describes in detail the INT $$^3$$ -Horus levels selected to implement the new architecture. These are the acquisition, segmentation, fusion, identification and Tracking levels. [ABSTRACT FROM AUTHOR]

Bronchopulmonary dysplasia (BPD) is the most common complication of preterm birth characterized by blunted post-natal lung development. BPD can be modelled in mice by exposure of newborn mouse pups to elevated oxygen levels. Little is known about the mechanisms of perturbed lung development associated with BPD. The advent of transgenic mice, where genetic rearrangements can be induced in particular cell-types at particular time-points during organogenesis, have great potential to explore the pathogenic mechanisms at play during arrested lung development. Many inducible, conditional transgenic technologies available rely on the application of the estrogen-receptor modulator, tamoxifen. While tamoxifen is well-tolerated and has been widely employed in adult mice, or in healthy developing mice; tamoxifen is not well-tolerated in combination with hyperoxia, in the most widely-used mouse model of BPD. To address this, we set out to establish a safe and effective tamoxifen dosing regimen that can be used in newborn mouse pups subjected to injurious stimuli, such as exposure to elevated levels of environmental oxygen. Our data reveal that a single intraperitoneal dose of tamoxifen of 0.2 mg applied to newborn mouse pups in 10 μl Miglyol vehicle was adequate to successfully drive Cre recombinase-mediated genome rearrangements by the fifth day of life, in a murine model of BPD. The number of recombined cells was comparable to that observed in regular tamoxifen administration protocols. These findings will be useful to investigators where tamoxifen dosing is problematic in the background of injurious stimuli and mouse models of human and veterinary disease. [ABSTRACT FROM AUTHOR]

Several observational studies have suggested an association between periodontitis and cerebral ischemia. This meta-analysis aimed to investigate whether this link exists, and if so, the degree to which it is significant. The Preferred Reporting Items for Systematic Reviews and Meta-analysis (PRISMA) guideline for systematic review was used. The search strategy included using electronic databases and hand searching works published up to March 2015. MEDLINE via PubMed, EMBASE, Proceedings Web of Science and Current Contents Connect were searched by two independent reviewers. Case-control, cross-sectional or cohort studies including patients with measures of periodontitis and ischemic stroke were eligible to be included in the analysis. Quality assessments of selected studies were performed. From a total of 414 titles and abstracts, 57 potentially relevant full text papers were identified. After inclusion criteria were applied, 8 studies were included in the present systematic review (5 case-control and 3 cohort studies). Although it was not the intention, cross-sectional studies were excluded due to eligibility criteria were not accomplished. Therefore, meta-analyses were conducted with data retrieved from the 8 studies included. These meta-analyses showed statistically significant association between periodontitis and ischemic stroke in both cohort pooled relative risks at 2.52 (1.77-3.58), and case-control studies pooled relative risks at 3.04 (1.10-8.43). In conclusion, the present meta-analysis demonstrated an association between periodontitis and ischemic stroke. However, well-designed prospective studies should be carried out to provide robust evidence of the link between both diseases. In regards to ischemic stroke subtypes, further case-control studies should be carried out to investigate whether there is any association between the different subtypes of cerebral infarcts and periodontitis. [ABSTRACT FROM AUTHOR]