Search

Genetic variability was studied in 78 populations of locally collected Vicia L. taxa for seed albumins, globulins and prolamins patterns by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) along with an ecogeographic characterization of sites investigated. 131, 119 and 98 bands were respectively used for albumin, globulin and prolamin cluster analysis. Dendrograms based on the Jaccard index and the UPGMA method were generated and the degree of genetic diversity between and within taxa was evaluated. Five clusters were generated from albumins, six from globulins and four from prolamins patterns. The results reflect the great diversity of storage proteins and a high correlation was obtained between the three studied fractions. Several accessions present specific bands which could be used as a discriminatory marker both on intra and interspecific levels. No clear relationships were seen between the groups according to their geographical origin. Data obtained from ecogeographic investigation can be used for future collecting missions. [ABSTRACT FROM AUTHOR]

Vicia sativa s.l. is one of the most commonly grown forage crop in Mediterranean regions. It is widely distributed and presents at the same time the largest overlaps and the most difficult taxonomic problems. The present study is an attempt to characterize the diversity and the taxonomic relationships among the members of a collection of 34 V. sativa s.l. from a variety of geographical sources of North Algeria with the aim to improve their conservation and management along with a synthesis of the results obtained by previous researchers. Four taxa ( obovata, cordata, consobrina and angustifolia) were analysed using 5 ecogeographic parameters and 78 morphological characters coded as multistate. The results reflect the great diversity of vetches and subspecies were not clearly recognizable from their plant morphology and ecogeography. Cluster analysis does not have provided clear separation among accessions. 11 morphological characters were excluded as they are homologous between all accessions. Important variation in standard colour, leaflets pairs per leaf, tendril length, hilum length and legume suture curvature was found in most accessions of the collection. Even for the characters that contribute the most to the separation of the accessions, the range of variation does show overlap between the subspecies. The study has drawn accessions from a wider geographical range and a distribution map of each taxa was produced. The information obtained can be used as a guide for future collecting missions. Variation in morphological characters appeared to be related to the site from which the accession was collected with 44.89 %. [ABSTRACT FROM AUTHOR]