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Background: Chemerin is a chemoattractant protein with adipokine and antimicrobial properties encoded by the retinoic acid receptor responder 2 (RARRES2) gene. Chemerin bioactivity largely depends on carboxyl-terminal proteolytic processing that generates chemerin isoforms with different chemotactic, regulatory, and antimicrobial potentials. While these mechanisms are relatively well known, the role of alternative splicing in generating isoform diversity remains obscure. Methods and results: Using rapid amplification of cDNA ends (RACE) PCR, we determined RARRES2 transcript variants present in mouse and human tissues and identified novel transcript variant 4 of mouse Rarres2 encoding mChem153K. Moreover, analyses of real-time quantitative PCR (RT-qPCR) and publicly-available next-generation RNA sequencing (RNA-seq) datasets showed that different alternatively spliced variants of mouse Rarres2 are present in mouse tissues and their expression patterns were unaffected by inflammatory and infectious stimuli except brown adipose tissue. However, only one transcript variant of human RARRES2 was present in liver and adipose tissue. Conclusion: Our findings indicate a limited role for alternative splicing in generating chemerin isoform diversity under all tested conditions. [ABSTRACT FROM AUTHOR]

In this paper, we report on mechanical properties observed during bending experiments conducted on quinary Ni–Mn–Ga–Co–Cu melt-spun ribbons. Depending on the ribbon's side to which force is applied, different mechanical response is noted. Substantially larger mechanical instabilities are observed when force is applied to the "free side" than to the "wheel side" of ribbons. When force is applied to the latter, much lower force fluctuations are recorded and the amplitude of the force–displacement response remains within the experimental resolution limit. It is also shown that the character of the force–displacement curve changes upon cycling; mainly by decreasing the maximum force and mechanical hysteresis. These results are important for materials design and optimization of the magnetic field-induced bending effect recently shown in Ni–Mn–Ga-based melt-spun ribbons. [ABSTRACT FROM AUTHOR]

The paper presents the photodegradation process of one-, two- and three-component dye mixtures by ZnO-SnO2 nanoparticles. After 60 min of running the processes, the dye removal efficiencies of 76.44, 72.69, 62.43, 77.00 and 92.46% for MB, RB, TB, MO and YQ degradation, respectively, were obtained. For binary and ternary systems, dye removal efficiencies for all cases exceeded 70%. When the binary and ternary dye mixtures were tested, the photodegradation efficiencies of ZnO-SnO2 were similar to those of the single mixtures, indicating that this material could be used in industrial applications in the future. The focus of the study was to investigate the effect of sorption on photodegradation efficiency and the presence of both cationic and anionic dyes on their degradation efficiency under UV light. The significance of the effect of sorption on the degradation efficiency allowing the interaction of the catalyst with the dyes removed was confirmed. The main factor influencing sorption and consequently photocatalysis was the nature of the dye. It was confirmed that the positively charged ZnO-SnO2 surface effectively sorbs the dyes and causes their degradation. [ABSTRACT FROM AUTHOR]

The effect of Cu addition on crystal structure, compressive properties and shape-memory effect of Ni50Mn25Ga25−xCux alloys was studied. With increasing Cu content, the type of crystal structure evolves following a sequence: L21 → 10M → 2M → 2M+γ. Addition of Cu significantly improves room temperature ductility. In polycrystalline Ni50Mn25Ga17Cu8 alloy, a full recoverable strain equal to 7 pct was achieved. High martensitic transformation temperature and large shape-memory effect makes this material potential candidate in high-temperature shape-memory applications. [ABSTRACT FROM AUTHOR]

The economic literature has for a long time been looking for explanations of a very strong international correlation of business cycles. This paper shows empirically that common fluctuations can to some degree be the effect of confidence shocks being transmitted internationally. We focus on a large (euro area) and a small, nearby economy (Poland). Our results show that euro area confidence fluctuations account for approximately 40–70% of business cycle fluctuations both in the euro area and in Poland. More importantly, their transmission happens not only via traditional channels (e.g., by confidence affecting euro area GDP and then Polish GDP via trade), but to a large extent occurs directly (e.g., by news spreading via media). [ABSTRACT FROM AUTHOR]

Next-generation sequencing (NGS) technologies together with an improved access to compute performance led to a cost-effective genome sequencing over the past several years. This allowed researchers to fully unleash the potential of genomic and metagenomic analyses to better elucidate two-way interactions between host cells and microbiome, both in steady-state and in pathological conditions. Experimental research involving metagenomics shows that skin resident microbes can influence the cutaneous pathophysiology. Here, we review metagenome approaches to study microbiota at this barrier site. We also describe the consequences of changes in the skin microbiota burden and composition, mostly revealed by these technologies, in the development of common inflammatory skin diseases. [ABSTRACT FROM AUTHOR]

Chemerin is a chemoattractant protein with adipokine properties encoded by the retinoic acid receptor responder 2 (RARRES2) gene. It has gained more attention in the past few years due to its multilevel impact on metabolism and immune responses. However, mechanisms controlling the constitutive and regulated expression of RARRES2 in a variety of cell types remain obscure. To our knowledge, this report is the first to show that DNA methylation plays an important role in the cell-specific expression of RARRES2 in adipocytes, hepatocytes, and B lymphocytes. Using luciferase reporter assays, we determined the proximal fragment of the RARRES2 gene promoter, located from − 252 to + 258 bp, to be a key regulator of transcription. Moreover, we showed that chemerin expression is regulated in murine adipocytes by acute-phase cytokines, interleukin 1β and oncostatin M. In contrast with adipocytes, these cytokines exerted a weak, if any, response in mouse hepatocytes, suggesting that the effects of IL-1β and OSM on chemerin expression is specific to fat tissue. Together, our findings highlight previously uncharacterized mediators and mechanisms that control chemerin expression. [ABSTRACT FROM AUTHOR]

Nowadays, the math learning is an important step in developing professional carriers in technical and economic sciences. Increasing the number of e-learning tools used in universities courses can reduce the potential barrier of access to mathematical knowledge, but most of them are not accessible for impaired students. Moreover, classical printed math books include little explicit instructional information about structural information interpretations. Taking into account these barriers the article presents the developed method used for creating interactive steps of decomposed math’s exercise solution and alternative description of math formulas accessible for the blind. The elements of proposed methodology: generation of state machine, design and presentation of transition conditions, generating the presentation layer and a typical usage by a blind user are presented. A set of rules for describing mathematical formulas were proposed after consultation with mathematicians and teachers of blind people. The application was developed as web application. The graphical interface of presented application was designed using PHP and JavaScript technologies. The collection of prepared exercises include about 240 prepared exercises from different areas of mathematics and 60 selected exercises including alternative description layer. About 1000 students and about 40 impaired students, from 6 faculties of the university use this platform during math courses for both self and class learning. The defined rules were used to read aloud mathematical formulas to the visually impaired people with a different level of mathematical knowledge. The results confirmed good understanding of mathematical formulas by using prepared alternative description. [ABSTRACT FROM AUTHOR]

Euro-area accession caused boom-bust cycles in several catching-up economies. Declining interest rates and easier financing conditions fuelled spending and borrowing from abroad. Over time inflation deteriorated external competitiveness, turning the boom into a bust. We ask whether such a scenario can be avoided using macroeconomic tools that are available in the period of joining a monetary union: central parity revaluation, fiscal tightening or increased taxation. We find that exchange rate revaluation is the most attractive option. It simultaneously trims the expansion of output and domestic demand, reduces the cost pressure and ranks first in terms of welfare. [ABSTRACT FROM AUTHOR]

Development of FPGA-based, network-enabled embedded systems in Register Transfer Level hardware description languages is tedious. Despite the automation of this process with numerous EDA tools available, no well-established design patterns exist. Moreover, the entire production cycle requires appropriate theoretical background and hardware design intuition from the developer which discourages the software community. To improve productivity and minimize time to market when assembling such systems, we propose a new hardware/software co-design approach to building reconfigurable hardware web services. The proposed integrated development platform features a programmable FPGA board where computations of different nature and purpose are logically distributed among a sequential soft-core processor program, a massively parallel accelerator and an independent communication module that handles remote clients' requests. Our second contribution is a set of tools that make the development of the aforementioned services essentially a software design undertaking with the extensive use of high-level programming languages. The platform has been tuned to act as a flexible runtime environment for image processing services, thus providing functionality of an intelligent camera. Two example services built from scratch according to the new methodology are discussed. Reduced development time and significant performance gain observed prove validity of the proposed approach and unveil a large potential of the assembled prototype. [ABSTRACT FROM AUTHOR]

Inflammatory processes and psychological states may mutually enhance each other as well as contribute to haemorheological alterations. The objective of the recent study was to determine blood rheological profile in patients with AD at different clinical stages. Blood rheology, as estimated by blood viscosity as well as deformability (elongation index—EI) and aggregation of erythrocytes (aggregation half time (AT1/2)—expressing the kinetic aspects and syllectogram amplitude (AMP)—representing total aggregation extent) were measured in 25 female AD patients, who showed clinical features of mild to severe AD and in 14 healthy subjects. There were no significant differences in blood rheological properties between patients with mild AD and the controls. A significant decrease in erythrocytes AT1/2 and AMP as well as EI were observed in severe AD patients as compared to other groups. Whole blood and plasma viscosity were similar in all groups. Both erythrocytes deformability and aggregation may be affected by pathophysiological processes associated with AD. Only AD patients with severe skin changes showed increased aggregability and decreased deformability of erythrocytes, suggesting that the phenomenon might be related to the disease activity. [ABSTRACT FROM AUTHOR]

Abstract  The increasing amounts of evidence prove that sex hormones modulate different cells function, including mast cells and lymphocytes. Dehydroepiandrosterone (DHEA) and its sulphate ester (DHEA-S) are both the secretory products of adrenal glands and the most abundant hormones in the systemic circulation of humans, converted then into androgens and estrogens in the periphery. DHEA may affect production of Th1 and Th2- associated cytokines, suggesting their significance in diseases where imbalanced lymphocyte activity plays the essential role, also in atopic diseases. Reduced circulating concentration of these androgens has been demonstrated in patients with immune-inflammatory diseases, including atopic eczema/dermatitis syndrome and asthma. It is unknown however, whether such changes contribute in any way to etiopathogenesis of the disorders, or are merely their consequences. Chronic urticaria, not included in the atopic triad, is a mast cell-dependent disease characterised by activation of autoimmune and inflammatory processes responding to different factors. Alterations in the immune-neuro-endocrine milieu may contribute to the development of the disease. It has been demonstrated that DHEA-S concentration is significantly lower in the peripheral circulation of patients suffering from chronic urticaria, as compared with healthy subjects. On one hand, this reflects some changes in hormonal homeostasis in the course of urticarial processes, yet on the other, suggests some possible role for this hormone in pathophysiology of the disease. In this brief review we present the available data on DHEA-S involvement in events associated with atopic allergy as well as chronic urticaria. [ABSTRACT FROM AUTHOR]

Abstract  Platelet activating factor (PAF) is a potent phospholipid mediator involved in anaphylaxis and chronic inflammatory disorders, including bronchial asthma. PAF is able to act both, directly as a chemotactic factor and indirectly through the release of other inflammatory agents. Apart from its known potent ability to activate platelets, PAF influences other immune and inflammatory cells function involved in asthma, which may be of importance in the pathogenesis of the disease. In addition, PAF administration can mimic some of abnormalities observed in asthma, including bronchoconstriction, bronchial hyper responsiveness, and gas exchange impairment, which may be mediated by leukotrienes acting as secondary mediators of some PAF effects. Therefore, there has been an extensive interest in the role of PAF in human asthma and major efforts have been continued to discover drugs acting thorough inhibition of PAF effects in the disease. Surprisingly, PAF receptor antagonists have not clearly proven their clinical benefits. It may appear that the combined blockage of PAF effects and other mediators involved in asthma is a way to improve clinical efficacy and also an interesting approach to control inflammation in the disease. This review will focus on two main issues: the role of PAF and PAF antagonists in asthma. [ABSTRACT FROM AUTHOR]

Oxidative stress is an important event in lesional skin of patients with chronic idiopathic urticaria (CIU). In the present study, we assessed blood oxidant/antioxidant status of patients suffering from CIU with positive response to autologous serum skin test (ASST) and with negative ASST, to improve our understanding of biological processes and the part of oxidative stress in this disease. Activities of manganese superoxide dismutase (MnSOD), copper–zinc superoxide dismutase (Cu/ZnSOD), glutathione peroxidase (GSH-PX), and catalase (CAT) as indices of enzymatic antioxidant capacity, as well as malondialdehyde (MDA) level as a maker of lipid peroxidation were measured in plasma and erythrocytes from 14 CIU female patients showing positive ASST, 31 CIU female patients with negative ASST and in 19 sex- and age- matched healthy subjects. The antioxidant enzyme activity in plasma and in erythrocytes did not differ significantly among the three groups. Also, the plasma and erythrocytes MDA levels were similar in the three groups. Based on our results, it seems that systemic activity of the enzymatic antioxidants (CuZn/SOD, MnSOD, GSH-Px, and CAT) as well as level of lipid peroxidation determined by MDA may not be increased in the course of immune-inflammatory processes associated with CIU. We also suggest that the systemic oxidant/antioxidant status of CIU patients, showing positive response to ASST, may not be different from that of CIU patients with negative ASST. [ABSTRACT FROM AUTHOR]

The evidence gathered has pointed to the fibrinolytic system, which apart from its major role in hemostasis may also be involved in inflammatory and immune processes. To understand better the role of fibrinolysis in urticaria, we measured plasma levels of the urokinase system associated molecules such as urokinase-type plasminogen activator (uPA), its soluble receptor (suPAR; CD87) and an inhibitor, plasminogen activator inhibitor type 1 (PAI-1) activity in chronic urticaria (CU) patients. Plasma was obtained from symptomatic sixteen CU patients (12 females and 4 males) showing positive response to autologous serum skin test (ASST), 28 CU patients with negative ASST (20 females and 8 males) as well as from healthy subjects matched by sex and age. The plasma level of uPA and suPAR antigens, PAI-1 activity did not differ significantly among the three subjects groups. The data obtained suggest that CU patients showing positive response to ASST have plasma profile of the urokinase system-associated proteins, which is not markedly different as compared with CU patients with negative ASST as well as healthy subjects. Our findings have also confirmed the earlier studies, suggesting that systemic fibrinolysis may not be involved in chronic urticaria. [ABSTRACT FROM AUTHOR]