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A Gram-positive, aerobic, rod-shaped bacterium, designated as strain 1605-214T, was isolated from the blood sample of a patient with cholangitis. Based on its 16S rRNA gene sequence, the strain 1605-214T belonged to the genus Cohnella and exhibited 97.9% sequence identity with Cohnella luojiensis DSM 24270T (GQ214052). DNA–DNA hybridization, digital DNA–DNA hybridization, and average nucleotide identity values between the two species were 23% ± 1.9, 21.1%, and 77.2%, respectively. The cellular fatty acids of strain 1605-214T were mainly comprised of anteiso-C15:0 (36.1%), iso-C16:0 (16.5%), and C16:0 (15.1%). The predominant quinone was menaquinone-7; predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, and aminophospholipid-1. The cell wall peptidoglycan of strain 1605-214T contained meso-diaminopimelic acid. DNA G + C content of strain 1605-214T was 50.6 mol%. 5187 genes out of a total of 5413 (94.6%) were assigned putative functions using eggNOG v5.0. Based on genotypic characteristics and genomic sequence analysis results, strain 1605-214T was confirmed to represent a novel species of genus Cohnella, for which the name Cohnella cholangitidis sp. nov., was proposed. [ABSTRACT FROM AUTHOR]

A new hydroxy fatty acid, named bombyxmoric acid methyl ester ( 1), along with three known ones, 12(S)-methyl 12-hydroxyoctadeca-9Z,13E,15Z-trienoate ( 2), 13(S)-methyl 13-hydroxyoctadeca-9Z, 11E-dienoate ( 3), and 13(S)-hydroxyoctadeca-9Z,11E-dienoic acid ( 4), were isolated from Bombyx mori droppings. The structure of compound 1 was established as methyl 7-hydroxyoctadeca-8E,10Z,15E-trienote on the basis of spectroscopic methods including HR-EI/MS, IR, 1D, and 2D NMR experiments. [ABSTRACT FROM AUTHOR]