1. SAD, a stearoyl-acyl carrier protein desaturase highly expressed in high-oil maize inbred lines.
- Author
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Liu, Zh. J., Yang, X. H., and Fu, Y.
- Subjects
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CORN , *GRAIN , *FATTY acids , *ENZYMES , *STEARIC acid , *OLEIC acid , *MESSENGER RNA , *PROTEINS - Abstract
As special maize with more than 6% oil concentration in the grain, high-oil maize has received increased interest recently. To date, little is known about the expressions of genes involved in fatty acid metabolism of high-oil maize. Stearoyl-acyl carrier protein desaturase (SAD) is a key enzyme that converts stearic acid to oleic acid. In this study, two-dimensional electrophoresis, gas chromatography, and real-time PCR were used to determine the expressions of SAD at three seed development stages in high-oil and normal maize inbred lines. SAD was significantly more abundantly expressed in high-oil maize than in normal maize, not only at the protein and mRNA levels, but also at the product level. These results suggested that a high expression of SAD may play an important role in increasing oil concentration in high-oil maize. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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