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Fe-N-C catalysts were prepared through metal-assisted polymerization method. Effects of carbon treatment, Fe loading, nitrogen source, and calcination temperature on the catalytic performance of the Fe-N-C for HO electroreduction were measured by voltammetry and chronoamperometry. The Fe-N-C catalyst shows optimal performance when prepared with pretreated active carbon, 0.2 wt.% Fe, paranitroaniline (4-NA) and one-time calcination. The Fe-N-C catalyst displayed good performance and stability for electroreduction of HO in alkaline solution. An Al-HO semi-fuel cell was set up with Fe-N-C catalyst as cathode and Al as anode. The cell exhibits an open-circuit voltage of 1.3 V and its power density reached 51.4 mW cm at 65 mA cm. [ABSTRACT FROM AUTHOR]