1. A near-infrared probe for non-invasively monitoring cerebrospinal fluid flow by 18F-positron emitting tomography and fluorescence.
- Author
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Guo, Hua, Kommidi, Harikrishna, Lekaye, Carl C., Koutcher, Jason, Judenhofer, Martin S., Cherry, Simon R., Wu, Amy P., Akin, Oguz, Souweidane, Mark M., Aras, Omer, Zhu, Zhaohui, and Ting, Richard
- Subjects
FLUID flow ,CEREBROSPINAL fluid ,FLUORESCENCE ,POSITRON emission tomography ,CEREBROSPINAL fluid examination ,CENTRAL nervous system ,TOMOGRAPHY - Abstract
Purpose: Knowing the precise flow of cerebrospinal fluid (CSF) is important in the management of multiple neurological diseases. Technology for non-invasively quantifying CSF flow would allow for precise localization of injury and assist in evaluating the viability of certain devices placed in the central nervous system (CNS). Methods: We describe a near-infrared fluorescent dye for accurately monitoring CSF flow by positron emission tomography (PET) and fluorescence. IR-783, a commercially available near-infrared dye, was chemically modified and radiolabeled with fluorine-18 to give [
18 F]-IR783-AMBF3 . [18 F]-IR783-AMBF3 was intrathecally injected into the rat models with normal and aberrant CSF flow and evaluated by the fluorescence and PET/MRI or PET/CT imaging modes. Results: IR783-AMBF3 was clearly distributed in CSF-containing volumes by PET and fluorescence. We compared IR783-AMBF3 (fluorescent at 778/793 nm, ex/em) to a shorter-wavelength, fluorescein equivalent (fluorescent at 495/511 nm, ex/em). IR783-AMBF3 was superior for its ability to image through blood (hemorrhage) and for imaging CSF-flow, through-skin, in subdural-run lumboperitoneal shunts. IR783-AMBF3 was safe under the tested dosage both in vitro and in vivo. Conclusion: The superior imaging properties of IR783-AMBF3 could lead to enhanced accuracy in the treatment of patients and would assist surgeons in non-invasively diagnosing diseases of the CNS. [ABSTRACT FROM AUTHOR]- Published
- 2020
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