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In the food matrix are involved very elemental products like water to other more complex like, for example, processed and non processed, vegetables and dairy products. All these matrix pass very restrictive controls and a intensive monitoring during the processing time to evaluate their safety and quality. The early detection of the contamination is critical to preserve the consumer's health and to avoid economic losses for the industry. On the other hand, microorganisms are part of a wide range and significant managing process, like fermentations used since ancient times and applied to many different matrix. One of this group of microorganism are Lactic Acid Bacteria (LAB) that play a different role, fermentative or contaminant, depending on the matrix where are founded. Electronic Noses (ENs) has shown to be a very effective and fast tool for monitoring microbiological spoilage and food quality control. The ability of this instrument can also be used for the selection of the most appropriate species or strains for a determinate purpose. The aim of this study was essay the ability of a novel EN for the detection of bacterial presence in water and other foodstuff in cooperation with classical microbiological and chemical techniques like Gas Chromatography Mass Spectrometry with SPME (GC-MS-SPME). The achieved results notably advocate the use of EN in industry laboratories like a very important tool in quality control.