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The purpose of this study was to develop kinds of multi-walled carbon nanotubes (MWCNT)-based materials, which could replace current autografts for neuron regeneration. The alternative MWCNT films was developed by encapsulated chitosan. To evaluate the characteristics of MWCNT films after modification, the measurements of electrical conductivity, contact angle and the degree of degradation were carried out. Two kinds of chitosan film preparation were designed, one was the dish film and the other was microscope slide film. The microscope slide film was exterior, however, the dish film was wrinkling. Comparing the thickness by micrometer caliper, the thickness of the dish film was 0.1950 to 0.0425 mm, and the microscope slide film was 0.0584 to 0.0109 mm. This result indicated the microscope slide film was more suitable for the following experiment because of less error value. The contact angle of the chitosan film was measured between 60~90 deg, it indicated the film was more hydrophobic. In order to achieve better biocompatibility, adding MWCNT significantly increased hydrophilicity of chitosan film.

One of the reason caused the production of scar is the excessive sediment or the overtime precipitation during the wounds healing. So far amount all the medical supplies, hydrocolloid dressings which used to repair the burn wounds and the normal trauma are bass on promotion of wound healing. Nevertheless there are no medical supplies combine hydrocolloid dressings with anti-pigmentation drugs; therefor our research has focused on preparing gelatin nanoparticle. First, prepared different size of particles by controlling the parameters. Second, loaded various concentrations of ascorbic acid into particles, analyzed the drug entrapment efficiency and the rate of drug release. Last, co-culture nanoparticle with fibroblast, research the effect of nanoparticle on the fibroblast, in order to prepare a successful anti pigmentation gelatin nanoparticle. Under distinct condition, particle size 150nm ~ 300nm of gelatin nanoparticle could be prepared, furthermore the effective concentration of ascorbic acid 0.3%~10% is successfully completed and the drug-loaded nanoparticles are able to extend the life-span of the fibroblast.