1. CSFV proliferation is associated with GBF1 and Rab2.
- Author
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Liang W, Zheng M, Bao C, and Zhang Y
- Subjects
- Animals, Brefeldin A pharmacology, Cell Proliferation drug effects, Cell Proliferation genetics, Classical Swine Fever Virus drug effects, Classical Swine Fever Virus pathogenicity, Golgi Apparatus drug effects, Golgi Apparatus genetics, Humans, Pyridines pharmacology, Quinolines pharmacology, RNA Interference, Swine genetics, Swine virology, Classical Swine Fever Virus genetics, Guanine Nucleotide Exchange Factors genetics, RNA genetics, rab2 GTP-Binding Protein genetics
- Abstract
The Golgi apparatus and its resident proteins are utilized and regulated by viruses to facilitate their proliferation. In this study, we investigated Classical swine fever virus (CSFV) proliferation when the function of the Golgi was disturbed. Golgi function was disturbed using chemical inhibitors, namely, brefeldin A (BFA) and golgicide A (GCA), and RNA interfering targets, such as the Golgi-specific BFA-resistance guanine nucleotide exchange factor 1 (GBF1) and Rab2 GTPases. CSFV proliferation was significantly inhibited during RNA replication and viral particle generation after BFA and GCA treatment. CSFV multiplication dynamics were retarded in cells transfected with GBF1 and Rab2 shRNA. Furthermore, CSFV proliferation was promoted by GBF1 and Rab2 overexpression using a lentiviral system. Hence, Golgi function is important for CSFV multiplication, and GBF1 and Rab2 participate in CSFV proliferation. Further studies must investigate Golgi-resident proteins to elucidate the mechanism underlying CSFV replication.
- Published
- 2017
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