1. Digital image processing: a useful tool in the analysis of lung injuries caused by chronic inhalation of agricultural herbicides
- Author
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Ana Karina Marques Salge, Ana Paula Alves Favareto, Francisco Assis da Silva, Renata Calciolari Rossi, Geovana Letícia Fernandes, Gabriela Vidotto Cavallieri, Claudio Pedrassoli Junior, Josiano Guilherme Puhle, Gisele Alborghetti Nai, Fabíola de Azevedo Mello, Débora Tavares de Resende e Silva, and Danillo Roberto Pereira
- Subjects
Male ,Adult male ,Health, Toxicology and Mutagenesis ,010501 environmental sciences ,01 natural sciences ,Digital image processing ,medicine ,Image Processing, Computer-Assisted ,Environmental Chemistry ,Animals ,Computational analysis ,Rats, Wistar ,Lung ,0105 earth and related environmental sciences ,Inhalation exposure ,Inflammation ,Ultrasonic nebulizers ,Inhalation ,business.industry ,Alveolar lesions ,Herbicides ,2,4-Dichlorophenoxyacetic acid ,Agriculture ,General Medicine ,Lung Injury ,Pollution ,Rats ,medicine.anatomical_structure ,Correlation analysis ,Herbicide ,Nuclear medicine ,business ,Research Article - Abstract
The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) is widely used in agriculture to control various weeds. The objective of this study was to use the digital image processing method to identify alveolar lesions in the lungs of rats submitted to chronic 2,4-dichlorophenoxyacetic acid (2,4-D) inhalation exposure. We used forty adult male Wistar rats. The rats were divided into four groups: control group (CG), low concentration group (LCG), medium concentration group (MCG), and high concentration group (HCG). In a 6-month exposure period, we used two boxes connected to ultrasonic nebulizers for herbicide spraying. After this period, the rats were euthanized for the collection and study of lung tissue. For each image, counts of injuries and blisters were performed automatically using a methodology based on digital image processing techniques. For analysis of the results, an electronic database (Excel®) was created. We used the Pearson method for correlation analysis; values of p
- Published
- 2021