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Start Over Search Limiters Peer Reviewed Topic cross-reactivity Publisher springer berlin heidelberg
21 results

7. A broadly cross-reactive monoclonal antibody against hepatitis E virus capsid antigen

Author

Kubickova, Barbara, Schenk, Jörg A., Ramm, Franziska, Markuškienė, Kornelija, Reetz, Jochen, Dremsek, Paul, Tamosiunas, Paulius Lukas, Cepulyte, Laima, Trinh, Hoai Anh, Scholz, Johannes, Memczak, Henry, Hovestädt, Marc, Ryll, René, Petraityte-Burneikiene, Rasa, Corman, Victor M., Andersson, Anika, Becher, Dietmar, Groschup, Martin H., Kubick, Stefan, Sellrie, Frank, Johne, Reimar, and Ulrich, Rainer G.

Published
2021
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20. Stochastic descriptors to study the fate and potential of naive T cell clonotypes in the periphery

Author

Carmen Molina-París, Martín López-García, Jesús R. Artalejo, and Antonio Gómez-Corral

Subjects
0301 basic medicine, Naive T cell, T-Lymphocytes, Population, Recent Thymic Emigrant, Receptors, Antigen, T-Cell, Biology, T-cell homeostasis, Models, Biological, Article, Procesos estocásticos, 03 medical and health sciences, 0302 clinical medicine, Modelling and Simulation, Homeostasis, education, Stochastic descriptor, education.field_of_study, Competition, Applied Mathematics, TCR diversity, T-cell receptor, Cross-reactivity, Extinction, Agricultural and Biological Sciences (miscellaneous), 60J28 Applications of continuous-time Markov processes on discrete state spaces, Clone Cells, 030104 developmental biology, T cell homeostasis, Stochastic univariate birth and death process, Evolutionary biology, Modeling and Simulation, Immunology, 92B05 General biology and biomathematics, 030215 immunology
Abstract

The population of naive T cells in the periphery is best described by determining both its T cell receptor diversity, or number of clonotypes, and the sizes of its clonal subsets. In this paper, we make use of a previously introduced mathematical model of naive T cell homeostasis, to study the fate and potential of naive T cell clonotypes in the periphery. This is achieved by the introduction of several new stochastic descriptors for a given naive T cell clonotype, such as its maximum clonal size, the time to reach this maximum, the number of proliferation events required to reach this maximum, the rate of contraction of the clonotype during its way to extinction, as well as the time to a given number of proliferation events. Our results show that two fates can be identified for the dynamics of the clonotype: extinction in the short-term if the clonotype experiences too hostile a peripheral environment, or establishment in the periphery in the long-term. In this second case the probability mass function for the maximum clonal size is bimodal, with one mode near one and the other mode far away from it. Our model also indicates that the fate of a recent thymic emigrant (RTE) during its journey in the periphery has a clear stochastic component, where the probability of extinction cannot be neglected, even in a friendly but competitive environment. On the other hand, a greater deterministic behaviour can be expected in the potential size of the clonotype seeded by the RTE in the long-term, once it escapes extinction.

Published
2016

21. Development of IgY-Based Sandwich Enzyme-Linked Immunosorbent Assay for the Detection of Vibrio parahaemolyticus

Author

Yuqing Dong, Li Wang, Qingping Zhong, and Binbin He

Subjects
Detection limit, Chromatography, biology, Chemistry, Vibrio parahaemolyticus, Ion chromatography, medicine.disease_cause, biology.organism_classification, Cross-reactivity, Microtiter plate, Sephadex, medicine, biology.protein, Immunoglobulin Y, Ammonium sulfate precipitation
Abstract

Vibrio parahaemolyticus is one of the most important food-borne pathogen which can cause serious infection in human. One kind of sandwich ELISA method was developed in this paper to detect V. parahaemolyticus rapidly and economically, using anti- V. parahaemolyticus IgY as capture antibody and rabbit anti- V. parahaemolyticus IgG as detection antibody. The specific IgY was harvested from egg yolk of immunized hens, then was purified by ammonium sulfate precipitation, Sephadex G-25 gel chromatography and DEAE-Sehparose FF ion exchange chromatography. The established assay had a limit of detection of 2.5×104 cfu/ml. The cross reactivity testing showed a high specificity for V. parahaemolyticus detection and other observed bacteria were found to be non-reactive. The IgY coated microtiter plate had high stability. Results suggest that IgY-based sandwich ELISA in this study provides a simple and convenient preparation for the development of immunoassay-kit and rapid detection.

Published
2012