7 results on '"Volker F. Wendisch"'
Search Results
2. De novo tryptophanase-based indole production by metabolically engineered Corynebacterium glutamicum
- Author
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Melanie Mindt, Lenny Ferrer, Dirk Bosch, Katarina Cankar, and Volker F. Wendisch
- Subjects
Corynebacterium glutamicum ,Indole ,Tryptophanase ,BIOS Applied Metabolic Systems ,Microbial fermentation ,Wageningen Plant Research ,General Medicine ,Applied Microbiology and Biotechnology ,Biotechnology - Abstract
Abstract Indole has an increasing interest in the flavor and fragrance industry. It is used in dairy products, tea drinks, and fine fragrances due to its distinct floral odor typical of jasmine blossoms. The current production of indole based on isolation from coal tar is non-sustainable and its isolation from plants is often unprofitable due to low yields. To offer an alternative to the conventional production, biosynthesis of indole has been studied recently. A glucose-based indole production was achieved by employing the Corynebacterium glutamicum tryptophan synthase α-subunit (TrpA) or indole-3-glycerol phosphate lyase (IGL) from wheat Triticum aestivum in a genetically-engineered C. glutamicum strain. In addition, a highly efficient bioconversion process using C. glutamicum heterologously expressing tryptophanase gene (tnaA) from Providencia rettgeri as a biocatalyst was developed. In this work, de novo indole production from glucose was enabled by expressing the P. rettgeri tnaA in a tryptophan-producing C. glutamicum strain. By metabolic engineering of a C. glutamicum shikimate accumulating base strain, tryptophan production of 2.14 ± 0.02 g L-1 was achieved. Introduction of the tryptophanase form P. rettgeri enabled indole production, but to low titers, which could be improved by sequestering indole into the water-immiscible solvent tributyrin during fermentation and a titer of 1.38 ± 0.04 g L-1 was achieved. The process was accelerated by decoupling growth from production increasing the volumetric productivity about 4-fold to 0.08 g L-1 h-1. Key points • Efficient de novo indole production via tryptophanases from glucose • Increased indole titers by product sequestration and improved precursor supply • Decoupling growth from production accelerated indole production
- Published
- 2023
3. Recent advances in the metabolic pathways and microbial production of coenzyme Q
- Author
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Fabien Pierrel, Arthur Burgardt, Jin-Ho Lee, Ludovic Pelosi, and Volker F. Wendisch
- Subjects
Saccharomyces cerevisiae Proteins ,Physiology ,Ubiquinone ,Humans ,food and beverages ,General Medicine ,Saccharomyces cerevisiae ,Applied Microbiology and Biotechnology ,Antioxidants ,Metabolic Networks and Pathways ,Biotechnology - Abstract
Coenzyme Q (CoQ) serves as an electron carrier in aerobic respiration and has become an interesting target for biotechnological production due to its antioxidative effect and benefits in supplementation to patients with various diseases. Here, we review discovery of the pathway with a particular focus on its superstructuration and regulation, and we summarize the metabolic engineering strategies for overproduction of CoQ by microorganisms. Studies in model microorganisms elucidated the details of CoQ biosynthesis and revealed the existence of multiprotein complexes composed of several enzymes that catalyze consecutive reactions in the CoQ pathways of Saccharomyces cerevisiae and Escherichia coli. Recent findings indicate that the identity and the total number of proteins involved in CoQ biosynthesis vary between species, which raises interesting questions about the evolution of the pathway and could provide opportunities for easier engineering of CoQ production. For the biotechnological production, so far only microorganisms have been used that naturally synthesize CoQ10 or a related CoQ species. CoQ biosynthesis requires the aromatic precursor 4-hydroxybenzoic acid and the prenyl side chain that defines the CoQ species. Up to now, metabolic engineering strategies concentrated on the overproduction of the prenyl side chain as well as fine-tuning the expression of ubi genes from the ubiquinone modification pathway, resulting in high CoQ yields. With expanding knowledge about CoQ biosynthesis and exploration of new strategies for strain engineering, microbial CoQ production is expected to improve.
- Published
- 2022
4. Detailed transcriptome analysis of the plant growth promoting Paenibacillus riograndensis SBR5 by using RNA-seq technology
- Author
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Luciana Fernandes de Brito, Marta Irla, Jörn Kalinowski, and Volker F. Wendisch
- Subjects
Promoter motifs ,Ribosome biding sites ,lcsh:QH426-470 ,Sequence Analysis, RNA ,lcsh:Biotechnology ,Gene Expression Profiling ,Plant Development ,RNA sequencing ,Thiamine pyrophosphate riboswitch Paenibacillus sonchi ,Plants ,Operon structures ,lcsh:Genetics ,RNA, Bacterial ,lcsh:TP248.13-248.65 ,Paenibacillus riograndensis ,Nucleotide Motifs ,5' Untranslated Regions ,Promoter Regions, Genetic ,Paenibacillus ,Transcriptional start sites ,Research Article - Abstract
Background The plant growth promoting rhizobacterium Paenibacillus riograndensis SBR5 is a promising candidate to serve as crop inoculant. Despite its potential in providing environmental and economic benefits, the species P. riograndensis is poorly characterized. Here, we performed for the first time a detailed transcriptome analysis of P. riograndensis SBR5 using RNA-seq technology. Results RNA was isolated from P. riograndensis SBR5 cultivated under 15 different growth conditions and combined together in order to analyze an RNA pool representing a large set of expressed genes. The resultant total RNA was used to generate 2 different libraries, one enriched in 5′-ends of the primary transcripts and the other representing the whole transcriptome. Both libraries were sequenced and analyzed to identify the conserved sequences of ribosome biding sites and translation start motifs, and to elucidate operon structures present in the transcriptome of P. riograndensis. Sequence analysis of the library enriched in 5′-ends of the primary transcripts was used to identify 1082 transcription start sites (TSS) belonging to novel transcripts and allowed us to determine a promoter consensus sequence and regulatory sequences in 5′ untranslated regions including riboswitches. A putative thiamine pyrophosphate dependent riboswitch upstream of the thiamine biosynthesis gene thiC was characterized by translational fusion to a fluorescent reporter gene and shown to function in P. riograndensis SBR5. Conclusions Our RNA-seq analysis provides insight into the P. riograndensis SBR5 transcriptome at the systems level and will be a valuable basis for differential RNA-seq analysis of this bacterium. © The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/)
- Published
- 2017
5. Journal Club: Calvin-Zyklus-Enzym reguliert Methanol-Stoffwechsel
- Author
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Volker F. Wendisch
- Published
- 2017
6. Roles of export genes cgmA and lysE for the production of L-arginine and L-citrulline by Corynebacterium glutamicum
- Author
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Hironori Taniguchi, João M. P. Jorge, Volker F. Wendisch, Dorit Lubitz, and Fernando Pérez-García
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0301 basic medicine ,Arginine ,Gene Expression ,chemical and pharmacologic phenomena ,Biology ,medicine.disease_cause ,Applied Microbiology and Biotechnology ,Corynebacterium glutamicum ,Microbiology ,Metabolic engineering ,03 medical and health sciences ,chemistry.chemical_compound ,Bacterial Proteins ,medicine ,Overproduction ,Escherichia coli ,Cadaverine ,Permease ,General Medicine ,030104 developmental biology ,Biochemistry ,chemistry ,Putrescine ,bacteria ,Amino Acid Transport Systems, Basic ,Citrulline ,Gene Deletion ,Biotechnology - Abstract
l-arginine is a semi-essential amino acid with application in cosmetic, pharmaceutical, and food industries. Metabolic engineering strategies have been applied for overproduction of l-arginine by Corynebacterium glutamicum. LysE was the only known l-arginine exporter of this bacterium. However, an l-arginine-producing strain carrying a deletion of lysE still accumulated about 10 mM l-arginine in the growth medium. Overexpression of the putative putrescine and cadaverine export permease gene cgmA was shown to compensate for the lack of lysE with regard to l-arginine export. Moreover, plasmid-borne overexpression of cgmA rescued the toxic effect caused by feeding of the dipeptide Arg-Ala to lysE-deficient C. glutamicum and argO-deficient Escherichia coli strains. Deletion of the repressor gene cgmR improved l-arginine titers by 5 %. Production of l-lysine and l-citrulline was not affected by cgmA overexpression. Taken together, CgmA may function as an export system not only for the diamine putrescine and cadaverine but also for l-arginine. The major export system for l-lysine and l-arginine LysE may also play a role in l-citrulline export since production of l-citrulline was reduced when lysE was deleted and improved by 45 % when lysE was overproduced.
- Published
- 2016
7. Amino Acid Biosynthesis – Pathways, Regulation and Metabolic Engineering
- Author
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Volker F. Wendisch and Volker F. Wendisch
- Subjects
- Amino acids, Protein engineering
- Abstract
Amino Acids Biosynthesis presents the current knowledge of fundamental as well as applied microbiology of amino acids. Topics discussed are the amino acid biosynthetic pathways, their genetic and biochemical regulation, transport of amino acids and genomics of producing microorganisms. The characterization of the control mechanisms of amino acid biosynthesis has revealed insights into principles of genetic and biochemical regulation, such as transcriptional regulators and a new class of regulatory elements, the riboswitch. The volume further deals with the metabolic engineering of microorganisms for the biotechnological production of amino acids for use as pharmaceuticals and, particularly, as food and feed additives. Comprehensive reviews are given of recent achievements to enable or improve production of amino acids and dipeptides by fermentation and enzyme catalysis. Here, the particular focus is on metabolic engineering, the rational improvement of metabolic functions using recombinant DNA technology.
- Published
- 2007
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