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3 results on '"Stepanov GA"'

1. Human fetal neural stem cells in rat brain: effects of preculturing and transplantation.

Author

Revishchin AV, Aleksandrova MA, Podgornyi OV, Marei MV, Poltavtseva RA, Korochkin LI, Stepanov GA, and Sukhikh GT

Subjects
Animals, Cell Movement, Embryo, Mammalian cytology, Embryo, Mammalian innervation, Fetus, Humans, Intermediate Filament Proteins analysis, Nerve Tissue Proteins analysis, Nestin, Neurons cytology, Neurons physiology, Rats, Stem Cells chemistry, Transplantation, Heterologous, Brain cytology, Cell Culture Techniques, Neurons transplantation, Stem Cell Transplantation, Stem Cells physiology
Abstract

The fate of human fetal stem/progenitor cells transplanted into rat brain depends on conditions of preculturing (long or short) and state and site of transplantation. Human nestin-positive stem cells cultured according to the short protocol did not migrate into hypoxic and normal brain after transplantation, but actively migrated in damaged spinal cord. After transplantation of long-cultured cells into the brain mainly committed neuroblasts and solitary nestin-positive cells migrated from the site of transplantation into the brain.

Published
2005
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2. Immunohistochemical study of fetal stem/progenitor cells from human brain transplanted into traumatized spinal cord of adult rats.

Author

Podgornyi OV, Marey MV, Karpenko DO, Aleksandrova MA, Poltavtseva RA, Revishchin AV, Stepanov GA, and Sukhikh GT

Subjects
Animals, Cell Differentiation physiology, Cell Movement physiology, Cells, Cultured, Humans, Immunohistochemistry, Neurons cytology, Neurons physiology, Rats, Rats, Wistar, Spinal Cord cytology, Stem Cells cytology, Spinal Cord pathology, Stem Cell Transplantation, Stem Cells metabolism, Transplantation, Heterologous
Abstract

Neural stem/progenitor cells from human fetal brain were grown in a tissue culture and transplanted into traumatized spinal cord of adult rats. The behavior and differentiation of transplanted cells were studied morphologically by means of histological and immunohistochemical methods and confocal microscopy. Human neural stem/progenitor cells were viable for not less than 3 months. They migrated and differentiated into neurons and glia in the traumatized spinal cord of adult rats.

Published
2004
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3. Xenotransplantation of stem/progenitor cells from human fetal brain to adult rats with spinal trauma.

Author

Stepanov GA, Karpenko DO, Aleksandrova MA, Podgornyi OV, Poltavtseva RA, Pevishchin AV, Marey MV, and Sukhikh GT

Subjects
Animals, Bisbenzimidazole metabolism, Cell Differentiation physiology, Cell Movement, Cell Survival, Female, Fluorescent Dyes metabolism, Humans, Intermediate Filament Proteins metabolism, Nerve Tissue Proteins metabolism, Nestin, Neuroglia cytology, Neuroglia metabolism, Neurons cytology, Neurons metabolism, Rats, Rats, Wistar, Spinal Cord cytology, Spinal Cord pathology, Spinal Cord Injuries pathology, Fetal Tissue Transplantation, Spinal Cord Injuries therapy, Stem Cell Transplantation, Stem Cells physiology, Transplantation, Heterologous
Abstract

In vitro grown neural stem cells from human fetal brain were transplanted to adult rats with spinal trauma. The spinal cord was examined morphologically using histological and immunohistochemical methods on days 5, 15, 30, and 110. Human neural stem/progenitor cells were viable, migrated, and differentiated into neurons and glia in the traumatized spinal cord in adult rats.

Published
2003
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