1. Isolation and Characterization of Bacillus cereus Bacteriophages from Foods and Soil.
- Author
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Oh H, Seo DJ, Jeon SB, Park H, Jeong S, Chun HS, Oh M, and Choi C
- Subjects
- Bacillus Phages classification, Bacillus Phages genetics, Bacillus Phages physiology, Host Specificity, Siphoviridae classification, Siphoviridae genetics, Siphoviridae physiology, Viral Proteins genetics, Viral Proteins metabolism, Bacillus Phages isolation & purification, Bacillus cereus virology, Food virology, Siphoviridae isolation & purification, Soil Microbiology
- Abstract
The aim of this study was to isolate and characterize Bacillus cereus bacteriophages of various origins. Twenty-seven bacteriophages against B. cereus were isolated from various Korean traditional fermented foods and soils. Plaque size, transmission electron microscopy, virulence profile, and in vitro lytic activity of bacteriophage isolates were examined. Transmission electron microscopy confirmed B. cereus bacteriophages belonging to the family Siphoviridae. Among B. cereus bacteriophages with broad host range, 18 isolates (66.7%) did not harbor any B. cereus virulence factors. Among them, bacteriophage strain CAU150036, CAU150038, CAU150058, CAU150064, CAU150065, and CAU150066 effectively inhibited B. cereus in vitro within 1 h. Therefore, they are considered potential candidates for controlling the contamination of B. cereus in food or other applications.
- Published
- 2017
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