1. Isoforms of protein kinase C and their distribution in human adrenal cortex and tumors.
- Author
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Mikosha AS, Tron'ko ND, Staren'kii DV, and Rybakov SI
- Subjects
- Adrenal Cortex Neoplasms enzymology, Blotting, Western, Cytosol enzymology, Densitometry, Humans, Hyperplasia enzymology, Isoenzymes biosynthesis, Microsomes enzymology, Protein Isoforms, Protein Kinase C-alpha, Protein Transport, Tissue Distribution, Adrenal Cortex metabolism, Adrenal Cortex Neoplasms chemistry, Protein Kinase C biosynthesis, Protein Kinase C chemistry
- Abstract
The cytosol and microsomal fractions of human adrenal cortex contain 3 isoforms of protein kinase C: alpha, zeta, and epsilon. The latter fraction is present in trace amounts. No isoforms beta1, beta2, gamma and delta were found in these cell fractions. The distribution of alpha-isoform between the cytosol and microsomal fraction is determined by tissue origin: in normal tissue its content differs by no more than 10%, while in most tumors this isoform is translocated into the microsomal fraction. The distribution of zeta-isoform did not depend on tissue origin.
- Published
- 2001
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