1. In vitro cytotoxic assessment of e-waste-related chemical pollution in impacted soil matrix.
- Author
-
Eze CT, Eze OO, Ugochukwu TE, Amaeze NH, Ogbuene EB, and Otitoloju AA
- Subjects
- Benin, China, Environmental Monitoring, Nigeria, Recycling, Soil, Electronic Waste analysis, Soil Pollutants analysis, Soil Pollutants toxicity
- Abstract
The environmental quality and toxicity of soil from some selected informal e-waste sites in West Africa was assessed on PLHC-1 liver cells. In addition, toxicity mechanisms such as apoptosis, necrosis and necroptosis were analysed in order to determine the effect of the actual chemical mixture present in the e-waste soil matrix. The investigation revealed that although e-waste soil extracts (polar and non-polar) and elutriates were significantly cytotoxic at the tested concentration (16 mg soil EQ/ml), PLHC-1 cell viability was not reduced below 50%. The non-polar extracts were more toxic compared to polar extracts and elutriates. The cytotoxic potency of soil from the informal e-waste-recycling sites ranged in this order: Alaba > Godome-Kouhounou > Agbogblosie. The study revealed that all e-waste soil extracts and elutriates induced significant (P < 0.01) PLHC-1 cell death by apoptosis and necrosis; however, cell death by apoptosis was higher compared to that by necrosis. The results indicated that except for non-polar extracts (N4, B4 and G4) from open burning areas that induced significant (P < 0.01) PLHC-1 cell death by necroptosis, other extracts and elutriates could not cause cell death by necroptosis. The study has demonstrated that soils from the Alaba e-waste site in Lagos could be more toxic than soils from Godome-Kouhounou (Cotonou) and Agbogblosie (Accra) e-waste sites and further highlighted open burning as an informal e-waste-handling method with greater negative impact on soil quality in the e-waste sites. The study emphasizes the urgent need for regulatory agencies to introduce regular residue-monitoring programmes in order to forestall the adverse effects of soil pollution episodes in the e-waste sites., (© 2022. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
- Published
- 2022
- Full Text
- View/download PDF