1. Plant growth-promoting bacterium Pseudomonas fluorescens FR1 secrets a novel type of extracellular polyhydroxybutyrate polymerase involved in abiotic stress response in plants
- Author
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Gabriela Soto, Ana Diez Tissera, Nicolás Daniel Ayub, and Margarita Stritzler
- Subjects
0301 basic medicine ,Chlorophyll ,030106 microbiology ,Biotecnología Agropecuaria ,Bioengineering ,Pseudomonas fluorescens ,POLYHYDROXYBUTYRATE (PHB) ,Applied Microbiology and Biotechnology ,Polyhydroxybutyrate ,03 medical and health sciences ,Bacterial Proteins ,Stress, Physiological ,ABIOTIC STRESS ,Extracellular ,Endophytes ,PHB POLYMERASE (PHBC) ,Secretion ,PLANTS ,Polymerase ,Triticum ,chemistry.chemical_classification ,biology ,Abiotic stress ,EXTRACELLULAR ,fungi ,food and beverages ,General Medicine ,biology.organism_classification ,030104 developmental biology ,Enzyme ,Biochemistry ,chemistry ,CIENCIAS AGRÍCOLAS ,Mutation ,biology.protein ,Biotecnología Agrícola y Biotecnología Alimentaria ,Bacteria ,Acyltransferases ,Genome, Bacterial ,Biotechnology - Abstract
Objectives: Identification of novel microbial factors contributing to plant protection against abiotic stress. Results: The genome of plant growth-promoting bacterium Pseudomonas fluorescens FR1 contains a short mobile element encoding a novel type of extracellular polyhydroxybutyrate (PHB) polymerase (PhbC) associated with a type I secretion system. Genetic analysis using a phbC mutant strain and plants showed that this novel extracellular enzyme is related to the PHB production in planta and suggests that PHB could be a beneficial microbial compound synthesized during plant adaptation to cold stress. Conclusion: Extracellular PhbC can be used as a new tool for improve crop production under abiotic stress. Fil: Stritzler, Margarita. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Diez Tissera, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina Fil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
- Published
- 2018