38 results on '"Lan, T."'
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2. Fear and Impact of COVID-19 Among Post-Infected Adults: Types and Associations with Quality of Life and Post-Traumatic Stress Symptoms
- Author
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Tinh X. Do, Ha-Linh Quach, Thi Ngoc Anh Hoang, Thao T. P. Nguyen, Lan T. H. Le, Tan T. Nguyen, Binh N. Do, Khue M. Pham, Vinh H. Vu, Linh V. Pham, Lien T. H. Nguyen, Hoang C. Nguyen, Tuan V. Tran, Trung H. Nguyen, Anh T. Nguyen, Hoan V. Nguyen, Phuoc B. Nguyen, Hoai T. T. Nguyen, Thu T. M. Pham, Thuy T. Le, Cuong Q. Tran, Kien T. Nguyen, Han T. Vo, and Tuyen Van Duong
- Subjects
COVID-19 survivors ,Health-related quality of life ,Fear ,Post-traumatic stress symptoms ,Latent profile analysis ,Public aspects of medicine ,RA1-1270 - Abstract
Abstract Survivors of COVID-19 are susceptible to diminished health-related quality of life (HRQoL) and adverse psychological health, which may be exacerbated by their experiences of fear and the impact of the pandemic itself. This study aims to identify distinct fear and impact patterns related to the COVID-19 pandemic among survivors through latent profile analysis (LPA) and examine the associations of fear and impact patterns with post-traumatic stress symptoms (PTSS) and HRQoL. A total of 5,890 Vietnamese COVID-19 survivors completed the COVID-19 Impact Battery– Disability Scale (CIB-D), the Fear of COVID-19 Scale (FCoV-19 S), the Impact of Event Scale-Revised for PTSS, and the 36-Item Short Form Survey (SF-36) for HRQoL. Four distinct groups of fear and impact were identified: “Fearful and highly impacted” (26.8%), “moderately impacted yet not fearful” (22.9%), “less impacted and less fearful” (18.6%), and “mildly impacted and neutral” (31.7%). Survivors who were “less impacted and less fearful” exhibited significantly higher HRQoL scores (regression coefficient, B: 10.9; 95% confidence interval (CI): 10.0 − 11.7), both in terms of physical (B: 12.0; 95%CI: 11.1 − 12.9) and mental health (B: 19.4; 95%CI: 9.6 − 11.1), and lower PTSS levels (B: -24.5; 95%CI: -25.8 − -23.3) compared to those who were “highly impacted and fearful”. It is imperative to acknowledge the intricate association between fear, impact, and mental health to comprehensively address the diverse needs of this distinct population post-COVID-19. These findings provide insights for designing interventions and support mechanisms for COVID-19 survivors.
- Published
- 2024
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3. Measurement of the charge asymmetry in top quark pair production in pp collisions at root s=7 TeV using the ATLAS detector
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G. AAD, E. ABAT, B. ABBOTT, J. ABDALLAH, A. A. ABDELALIM, A. ABDESSELAM, O. ABDINOV, B. ABI, M. ABOLINS, H. ABRAMOWICZ, H. ABREU, E. ACERBI, B. S. ACHARYA, M. ACKERS, D. L. ADAMS, T. N. ADDY, J. ADELMAN, M. ADERHOLZ, C. ADORISIO, P. ADRAGNA, T. ADYE, S. AEFSKY, J. A. AGUILAR SAAVEDRA, M. AHARROUCHE, S. P. AHLEN, F. AHLES, A. AHMAD, H. AHMED, M. AHSAN, G. AIELLI, T. AKDOGAN, P. F. AKESSON, T. P. A. AKESSON, G. AKIMOTO, A. V. AKIMOV, A. AKTAS, M. S. ALAM, M. A. ALAM, J. ALBERT, S. ALBRAND, M. ALEKSA, I. N. ALEKSANDROV, M. ALEPPO, F. ALESSANDRIA, C. ALEXA, G. ALEXANDER, G. ALEXANDRE, T. ALEXOPOULOS, M. ALHROOB, M. ALIEV, G. ALIMONTI, J. ALISON, M. ALIYEV, P. P. ALLPORT, S. E. ALLWOOD SPIERS, J. ALMOND, A. ALOISIO, R. ALON, A. ALONSO, J. ALONSO, M. G. ALVIGGI, K. AMAKO, P. AMARAL, G. AMBROSINI, G. AMBROSIO, C. AMELUNG, V. V. AMMOSOV, A. AMORIM, G. AMOROS, N. AMRAM, C. ANASTOPOULOS, T. ANDEEN, C. F. ANDERS, K. J. ANDERSON, A. ANDREAZZA, V. ANDREI, M. L. ANDRIEUX, X. S. ANDUAGA, A. ANGERAMI, F. ANGHINOLFI, N. ANJOS, A. ANNOVI, A. ANTONAKI, M. ANTONELLI, S. ANTONELLI, J. ANTOS, B. ANTUNOVIC, F. ANULLI, S. AOUN, G. ARABIDZE, I. ARACENA, Y. ARAI, A. T. H. ARCE, J. P. ARCHAMBAULT, S. ARFAOUI, J. F. ARGUIN, T. ARGYROPOULOS, E. ARIK, M. ARIK, A. J. ARMBRUSTER, K. E. ARMS, S. R. ARMSTRONG, O. ARNAEZ, C. ARNAULT, A. ARTAMONOV, D. ARUTINOV, M. ASAI, S. ASAI, R. ASFANDIYAROV, S. ASK, B. ASMAN, D. ASNER, L. ASQUITH, K. ASSAMAGAN, A. ASTBURY, A. ASTVATSATOUROV, B. ATHAR, G. ATOIAN, B. AUBERT, B. AUERBACH, E. AUGE, K. AUGSTEN, M. AUROUSSEAU, N. AUSTIN, G. AVOLIO, R. AVRAMIDOU, D. AXEN, C. AY, G. AZUELOS, Y. AZUMA, M. A. BAAK, G. BACCAGLIONI, C. BACCI, A. M. BACH, H. BACHACOU, K. BACHAS, G. BACHY, M. BACKES, E. BADESCU, P. BAGNAIA, Y. BAI, D. C. BAILEY, T. BAIN, J. T. BAINES, O. K. BAKER, M. D. BAKER, S. BAKER, F. BALTASAR DOS SANTOS PEDROSA, E. BANAS, P. BANERJEE, S. BANERJEE, D. BANFI, A. BANGERT, V. BANSAL, S. P. BARANOV, S. BARANOV, A. BARASHKOU, T. BARBER, E. L. BARBERIO, D. BARBERIS, M. BARBERO, D. Y. BARDIN, T. BARILLARI, M. BARISONZI, T. BARKLOW, N. BARLOW, B. M. BARNETT, R. M. BARNETT, A. BARONCELLI, M. BARONE, A. J. BARR, F. BARREIRO, J. BARREIRO GUIMARAES DA COSTA, P. BARRILLON, V. BARTHELD, H. BARTKO, R. BARTOLDUS, D. BARTSCH, R. L. BATES, S. BATHE, L. BATKOVA, J. R. BATLEY, A. BATTAGLIA, M. BATTISTIN, G. BATTISTONI, F. BAUER, H. S. BAWA, M. BAZALOVA, B. BEARE, T. BEAU, P. H. BEAUCHEMIN, R. BECCHERLE, N. BECERICI, P. BECHTLE, G. A. BECK, H. P. BECK, M. BECKINGHAM, K. H. BECKS, A. J. BEDDALL, A. BEDDALL, V. A. BEDNYAKOV, C. BEE, M. BEGEL, S. BEHAR HARPAZ, P. K. BEHERA, M. BEIMFORDE, G. A. N. BELANGER, C. BELANGER CHAMPAGNE, B. BELHORMA, P. J. BELL, W. H. BELL, G. BELLA, L. BELLAGAMBA, F. BELLINA, G. BELLOMO, M. BELLOMO, A. BELLONI, K. BELOTSKIY, O. BELTRAMELLO, A. BELYMAM, S. BEN AMI, O. BENARY, D. BENCHEKROUN, C. BENCHOUK, M. BENDEL, B. H. BENEDICT, N. BENEKOS, Y. BENHAMMOU, G. P. BENINCASA, D. P. BENJAMIN, M. BENOIT, J. R. BENSINGER, K. BENSLAMA, S. BENTVELSEN, M. BERETTA, D. BERGE, E. BERGEAAS KUUTMANN, N. BERGER, F. BERGHAUS, E. BERGLUND, J. BERINGER, K. BERNARDET, P. BERNAT, R. BERNHARD, C. BERNIUS, T. BERRY, F. BERTINELLI, S. BERTOLUCCI, M. I. BESANA, N. BESSON, S. BETHKE, R. M. BIANCHI, M. BIANCO, O. BIEBEL, M. BIERI, J. BIESIADA, M. BIGLIETTI, H. BILOKON, M. BINDER, S. BINET, A. BINGUL, C. BINI, C. BISCARAT, R. BISCHOF, U. BITENC, K. M. BLACK, R. E. BLAIR, O. BLANCH, J. B. BLANCHARD, G. BLANCHOT, C. BLOCKER, J. BLOCKI, A. BLONDEL, W. BLUM, U. BLUMENSCHEIN, C. BOARETTO, G. J. BOBBINK, A. BOCCI, D. BOCIAN, R. BOCK, M. BOEHLER, M. BOEHM, J. BOEK, N. BOELAERT, S. BOSER, J. A. BOGAERTS, A. BOGOUCH, C. BOHM, J. BOHM, V. BOISVERT, T. BOLD, V. BOLDEA, A. BOLDYREV, V. G. BONDARENKO, M. BONDIOLI, R. BONINO, M. BOONEKAMP, G. BOORMAN, M. BOOSTEN, C. N. BOOTH, P. S. L. BOOTH, P. BOOTH, J. R. A. BOOTH, S. BORDONI, C. BORER, K. BORER, A. BORISOV, G. BORISSOV, I. BORJANOVIC, S. BORRONI, K. BOS, D. BOSCHERINI, M. BOSMAN, H. BOTERENBROOD, D. BOTTERILL, J. BOUCHAMI, J. BOUDREAU, E. V. BOUHOVA THACKER, C. BOULAHOUACHE, C. BOURDARIOS, A. BOVEIA, J. BOYD, B. H. BOYER, I. R. BOYKO, N. I. BOZHKO, I. BOZOVIC JELISAVCIC, S. BRACCINI, J. BRACINIK, A. BRAEM, E. BRAMBILLA, P. BRANCHINI, G. W. BRANDENBURG, A. BRANDT, G. BRANDT, O. BRANDT, U. BRATZLER, B. BRAU, J. E. BRAU, H. M. BRAUN, S. BRAVO, B. BRELIER, J. BREMER, R. BRENNER, S. BRESSLER, D. BRETON, N. D. BRETT, P. G. BRIGHT THOMAS, D. BRITTON, F. M. BROCHU, I. BROCK, R. BROCK, T. J. BRODBECK, E. BRODET, F. BROGGI, C. BROMBERG, G. BROOIJMANS, W. K. BROOKS, G. BROWN, E. BRUBAKER, P. A. BRUCKMAN DE RENSTROM, D. BRUNCKO, R. BRUNELIERE, S. BRUNET, A. BRUNI, G. BRUNI, M. BRUSCHI, T. BUANES, F. BUCCI, J. BUCHANAN, N. J. BUCHANAN, P. BUCHHOLZ, A. G. BUCKLEY, I. A. BUDAGOV, B. BUDICK, V. BUSCHER, L. BUGGE, D. BUIRA CLARK, E. J. BUIS, F. BUJOR, O. BULEKOV, M. BUNSE, T. BURAN, H. BURCKHART, S. BURDIN, T. BURGESS, S. BURKE, E. BUSATO, P. BUSSEY, C. P. BUSZELLO, F. BUTIN, B. BUTLER, J. M. BUTLER, C. M. BUTTAR, J. M. BUTTERWORTH, T. BYATT, J. CABALLERO, S. CABRERA URBAN, M. CACCIA, O. CAKIR, P. CALAFIURA, G. CALDERINI, P. CALFAYAN, R. CALKINS, L. P. CALOBA, R. CALOI, D. CALVET, A. CAMARD, P. CAMARRI, M. CAMBIAGHI, D. CAMERON, J. CAMMIN, S. CAMPANA, M. CAMPANELLI, V. CANALE, F. CANELLI, A. CANEPA, J. CANTERO, L. CAPASSO, M. D. M. CAPEANS GARRIDO, I. CAPRINI, M. CAPRINI, M. CAPRIO, M. CAPUA, R. CAPUTO, C. CARAMARCU, R. CARDARELLI, L. CARDIEL SAS, T. CARLI, G. CARLINO, L. CARMINATI, B. CARON, S. CARON, C. CARPENTIERI, G. D. CARRILLO MONTOYA, S. CARRON MONTERO, A. A. CARTER, J. R. CARTER, J. CARVALHO, D. CASADEI, M. P. CASADO, M. CASCELLA, C. CASO, A. M. CASTANEDA HERNADEZ, E. CASTANEDA MIRANDA, V. CASTILLO GIMENEZ, N. F. CASTRO, F. CASTROVILLARI, G. CATALDI, F. CATANEO, A. CATINACCIO, J. R. CATMORE, A. CATTAI, G. CATTANI, S. CAUGHRON, D. CAUZ, A. CAVALLARI, P. CAVALLERI, D. CAVALLI, M. CAVALLI SFORZA, V. CAVASINNI, A. CAZZATO, F. CERADINI, C. CERNA, A. S. CERQUEIRA, A. CERRI, L. CERRITO, F. CERUTTI, M. CERVETTO, S. A. CETIN, F. CEVENINI, A. CHAFAQ, D. CHAKRABORTY, K. CHAN, J. D. CHAPMAN, J. W. CHAPMAN, E. CHAREYRE, D. G. CHARLTON, S. CHARRON, S. CHATTERJII, V. CHAVDA, S. CHEATHAM, S. CHEKANOV, S. V. CHEKULAEV, G. A. CHELKOV, H. CHEN, L. CHEN, S. CHEN, T. CHEN, X. CHEN, S. CHENG, A. CHEPLAKOV, V. F. CHEPURNOV, R. CHERKAOUI EL MOURSLI, V. TCHERNIATINE, D. CHESNEANU, E. CHEU, S. L. CHEUNG, L. CHEVALIER, F. CHEVALLIER, V. CHIARELLA, G. CHIEFARI, L. CHIKOVANI, J. T. CHILDERS, A. CHILINGAROV, G. CHIODINI, V. CHIZHOV, G. CHOUDALAKIS, S. CHOURIDOU, T. CHRISTIANSEN, I. A. CHRISTIDI, A. CHRISTOV, D. CHROMEK BURCKHART, M. L. CHU, J. CHUDOBA, G. CIAPETTI, E. CICALINI, A. K. CIFTCI, R. CIFTCI, D. CINCA, V. CINDRO, M. D. CIOBOTARU, C. CIOCCA, A. CIOCIO, M. CIRILLI, M. CITTERIO, A. CLARK, P. J. CLARK, W. CLELAND, J. C. CLEMENS, B. CLEMENT, C. CLEMENT, D. CLEMENTS, R. W. CLIFFT, Y. COADOU, M. COBAL, A. COCCARO, J. COCHRAN, R. COCO, P. COE, S. COELLI, J. COGGESHALL, E. COGNERAS, C. D. COJOCARU, J. COLAS, B. COLE, A. P. COLIJN, C. COLLARD, N. J. COLLINS, C. COLLINS TOOTH, J. COLLOT, G. COLON, R. COLUCCIA, G. COMUNE, P. CONDE MUINO, E. CONIAVITIS, M. CONSONNI, S. CONSTANTINESCU, C. CONTA, F. CONVENTI, J. COOK, M. COOKE, B. D. COOPER, A. M. COOPER SARKAR, N. J. COOPER SMITH, K. COPIC, T. CORNELISSEN, M. CORRADI, S. CORREARD, F. CORRIVEAU, A. CORSO RADU, A. CORTES GONZALEZ, G. CORTIANA, G. COSTA, M. J. COSTA, D. COSTANZO, T. COSTIN, D. COTE, R. COURA TORRES, L. COURNEYEA, C. COUYOUMTZELIS, G. COWAN, C. COWDEN, B. E. COX, K. CRANMER, J. CRANSHAW, M. CRISTINZIANI, G. CROSETTI, R. CRUPI, S. CREPE RENAUDIN, C. CUENCA ALMENAR, T. CUHADAR DONSZELMANN, S. CUNEO, A. CUNHA, M. CURATOLO, C. J. CURTIS, P. CWETANSKI, Z. CZYCZULA, S. D'AURIA, M. D'ONOFRIO, A. D'ORAZIO, A. DA ROCHA GESUALDI MELLO, P. V. M. DA SILVA, C. DA VIA, W. DABROWSKI, A. DAHLHOFF, T. DAI, C. DALLAPICCOLA, S. J. DALLISON, J. DALMAU, C. H. DALY, M. DAM, M. DAMERI, H. O. DANIELSSON, R. DANKERS, D. DANNHEIM, V. DAO, G. DARBO, G. L. DARLEA, C. DAUM, J. P. DAUVERGNE, W. DAVEY, T. DAVIDEK, D. W. DAVIDSON, N. DAVIDSON, R. DAVIDSON, M. DAVIES, A. R. DAVISON, I. DAWSON, J. W. DAWSON, R. K. DAYA, K. DE, R. DE ASMUNDIS, P. E. DE CASTRO FARIA SALGADO, S. DE CECCO, J. DE GRAAT, N. DE GROOT, P. DE JONG, E. DE LA CRUZ BURELO, C. DE LA TAILLE, B. DE LOTTO, L. DE MORA, M. DE OLIVEIRA BRANCO, D. DE PEDIS, P. DE SAINTIGNON, A. DE SALVO, U. DE SANCTIS, A. DE SANTO, J. B. DE VIVIE DE REGIE, G. DE ZORZI, S. DEAN, H. DEBERG, G. DEDES, D. V. DEDOVICH, P. O. DEFAY, J. DEGENHARDT, M. DEHCHAR, M. DEILE, C. DEL PAPA, J. DEL PESO, T. DEL PRETE, A. DELL'ACQUA, L. DELL'ASTA, M. DELLA PIETRA, D. DELLA VOLPE, M. DELMASTRO, P. DELPIERRE, N. DELRUELLE, P. A. DELSART, C. DELUCA, S. DEMERS, M. DEMICHEV, B. DEMIRKOZ, J. DENG, W. DENG, S. P. DENISOV, C. DENNIS, J. E. DERKAOUI, F. DERUE, P. DERVAN, K. DESCH, P. O. DEVIVEIROS, A. DEWHURST, B. DEWILDE, S. DHALIWAL, R. DHULLIPUDI, A. DI CIACCIO, L. DI CIACCIO, A. DI DOMENICO, A. DI GIROLAMO, B. DI GIROLAMO, S. DI LUISE, A. DI MATTIA, R. DI NARDO, A. DI SIMONE, M. A. DIAZ, M. M. DIAZ GOMEZ, F. DIBLEN, E. B. DIEHL, H. DIETL, J. DIETRICH, T. A. DIETZSCH, S. DIGLIO, K. DINDAR YAGCI, D. J. DINGFELDER, C. DIONISI, R. DIPANJAN, P. DITA, S. DITA, F. DITTUS, F. DJAMA, R. DJILKIBAEV, T. DJOBAVA, M. A. B. DO VALE, A. DO VALLE WEMANS, T. K. O. DOAN, M. DOBBS, R. DOBINSON, D. DOBOS, E. DOBSON, M. DOBSON, J. DODD, O. B. DOGAN, C. DOGLIONI, T. DOHERTY, Y. DOI, J. DOLEJSI, I. DOLENC, Z. DOLEZAL, B. A. DOLGOSHEIN, T. DOHMAE, E. DOMINGO, M. DONEGA, J. DONINI, J. DOPKE, A. DORIA, A. DOS ANJOS, M. DOSIL, A. DOTTI, M. T. DOVA, J. D. DOWELL, A. DOXIADIS, A. T. DOYLE, J. DRAGIC, D. DRAKOULAKOS, Z. DRASAL, J. DREES, N. DRESSNANDT, H. DREVERMANN, C. DRIOUICHI, M. DRIS, J. G. DROHAN, J. DUBBERT, T. DUBBS, E. DUCHOVNI, G. DUCKECK, A. DUDAREV, F. DUDZIAK, M. DUHRSSEN, H. DUR, I. P. DUERDOTH, L. DUFLOT, M. A. DUFOUR, M. DUNFORD, H. DURAN YILDIZ, A. DUSHKIN, R. DUXFIELD, M. DWUZNIK, F. DYDAK, D. DZAHINI, M. DUREN, W. L. EBENSTEIN, J. EBKE, S. ECKERT, S. ECKWEILER, K. EDMONDS, C. A. EDWARDS, I. EFTHYMIOPOULOS, K. EGOROV, W. EHRENFELD, T. EHRICH, T. EIFERT, G. EIGEN, K. EINSWEILER, E. EISENHANDLER, T. EKELOF, M. EL KACIMI, M. ELLERT, S. ELLES, F. ELLINGHAUS, K. ELLIS, N. ELLIS, J. ELMSHEUSER, M. ELSING, R. ELY, D. EMELIYANOV, R. ENGELMANN, A. ENGL, B. EPP, A. EPPIG, J. ERDMANN, A. EREDITATO, V. EREMIN, D. ERIKSSON, I. ERMOLINE, J. ERNST, M. ERNST, J. ERNWEIN, D. ERREDE, S. ERREDE, E. ERTEL, M. ESCALIER, C. ESCOBAR, X. ESPINAL CURULL, B. ESPOSITO, F. ETIENNE, A. I. ETIENVRE, E. ETZION, H. EVANS, V. N. EVDOKIMOV, C. FABRE, K. FACIUS, R. M. FAKHRUTDINOV, S. FALCIANO, A. C. FALOU, Y. FANG, M. FANTI, A. FARBIN, A. FARILLA, J. FARLEY, T. FAROOQUE, S. M. FARRINGTON, P. FARTHOUAT, P. FASSNACHT, D. FASSOULIOTIS, B. FATHOLAHZADEH, L. FAYARD, F. FAYETTE, R. FEBBRARO, P. FEDERIC, O. L. FEDIN, I. FEDORKO, W. 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Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de l'Accélérateur Linéaire (LAL), Université Paris-Sud - Paris 11 (UP11)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Annecy de Physique des Particules (LAPP), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS), Centre de Physique des Particules de Marseille (CPPM), Aix Marseille Université (AMU)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche 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SMIRNOVA, B.C. SMITH, D. SMITH, K.M. SMITH, M. SMIZANSKA, K. SMOLEK, A.A. SNESAREV, S.W. SNOW, J. SNOW, J. SNUVERINK, S. SNYDER, M. SOARES, R. SOBIE, J. SODOMKA, A. SOFFER, C.A. SOLANS, M. SOLAR, J. SOLC, E. SOLFAROLI CAMILLOCCI, A.A. SOLODKOV, O.V. SOLOVYANOV, R. SOLUK, J. SONDERICKER, V. SOPKO, B. SOPKO, M. SORBI, M. SOSEBEE, A. SOUKHAREV, S. SPAGNOLO, F. SPANO, P. SPECKMAYER, E. SPENCER, R. SPIGHI, G. SPIGO, F. SPILA, E. SPIRITI, R. SPIWOKS, L. SPOGLI, M. SPOUSTA, T. SPREITZER, B. SPURLOCK, R.D.ST. DENIS, T. STAHL, J. STAHLMAN, R. STAMEN, S.N. STANCU, E. STANECKA, R.W. STANEK, C. STANESCU, S. STAPNES, E.A. STARCHENKO, J. STARK, P. STAROBA, P. STAROVOITOV, J. STASTNY, A. STAUDE, P. STAVINA, G. STAVROPOULOS, G. STEELE, E. STEFANIDIS, P. STEINBACH, P. STEINBERG, I. STEKL, B. STELZER, H.J. STELZER, O. STELZER-CHILTON, H. STENZEL, K. STEVENSON, G. STEWART, T.D. STEWART, W. STILLER, T. STOCKMANNS, M.C. STOCKTON, M. STODULSKI, K. STOERIG, G. STOICEA, S. STONJEK, P. STRACHOTA, A.R. STRADLING, A. STRAESSNER, J. STRANDBERG, S. STRANDBERG, A. STRANDLIE, M. STRAUSS, D. STRIEGEL, P. STRIZENEC, R. STROHMER, D.M. STROM, J.A. STRONG, R. STROYNOWSKI, J. STRUBE, B. STUGU, I. STUMER, D.A. SOH, D. SU, S. SUBRAMANIA, Y. SUGAYA, T. SUGIMOTO, C. SUHR, M. SUK, V.V. SULIN, S. SULTANSOY, T. SUMIDA, X.H. SUN, J.E. SUNDERMANN, K. SURULIZ, S. SUSHKOV, G. SUSINNO, M.R. SUTTON, T. SUZUKI, Y. SUZUKI, YU.M. SVIRIDOV, I. SYKORA, T. SYKORA, R.R. SZCZYGIEL, B. SZELESS, T. SZYMOCHA, J. SANCHEZ, D. TA, S. TABOADA GAMEIRO, K. TACKMANN, A. TAFFARD, R. TAFIROUT, A. TAGA, Y. TAKAHASHI, H. TAKAI, R. TAKASHIMA, H. TAKEDA, T. TAKESHITA, M. TALBY, A. TALYSHEV, M.C. TAMSETT, J. TANAKA, R. TANAKA, S. TANAKA, Y. TANAKA, G.P. TAPPERN, S. TAPPROGGE, D. TARDIF, S. TAREM, F. TARRADE, G.F. TARTARELLI, P. TAS, M. TASEVSKY, E. TASSI, M. TATARKHANOV, Y. TAYALATI, C. TAYLOR, F.E. TAYLOR, G. TAYLOR, G.N. TAYLOR, R.P. TAYLOR, W. TAYLOR, P. TEIXEIRA-DIAS, H. TEN KATE, P.K. TENG, Y.D. TENNENBAUM-KATAN, R. TER-ANTONYAN, S. TERADA, K. TERASHI, J. TERRON, M. TERWORT, M. TESTA, R.J. TEUSCHER, C.M. TEVLIN, J. THADOME, M. THIOYE, S. THOMA, A. THOMAS, J.P. THOMAS, E.N. THOMPSON, P.D. THOMPSON, R.J. THOMPSON, A.S. THOMPSON, E. THOMSON, R.P. THUN, T. TIC, V.O. TIKHOMIROV, Y.A. TIKHONOV, S. TIMM, C.J.W.P. TIMMERMANS, P. TIPTON, F.J. TIQUE AIRES VIEGAS, S. TISSERANT, J. TOBIAS, B. TOCZEK, T. TODOROV, S. TODOROVA-NOVA, B. TOGGERSON, J. TOJO, S. TOKAR, K. TOKUSHUKU, K. TOLLEFSON, L. TOMASEK, M. TOMASEK, M. TOMOTO, D. TOMPKINS, L. TOMPKINS, K. TOMS, A. TONAZZO, G. TONG, A. TONOYAN, C. TOPFEL, N.D. TOPILIN, E. TORRENCE, E. TORRO PASTOR, J. TOTH, F. TOUCHARD, D.R. TOVEY, T. TREFZGER, J. TREIS, L. TREMBLET, A. TRICOLI, I.M. TRIGGER, G. TRILLING, S. TRINCAZ-DUVOID, T.N. TRINH, M.F. TRIPIANA, N. TRIPLETT, W. TRISCHUK, A. TRIVEDI, Z. TRKA, B. TROCME, C. TRONCON, A. TRZUPEK, C. TSAROUCHAS, J.C.-L. TSENG, M. TSIAKIRIS, P.V. TSIARESHKA, D. TSIONOU, G. TSIPOLITIS, V. TSISKARIDZE, E.G. TSKHADADZE, I.I. TSUKERMAN, V. TSULAIA, J.-W. TSUNG, S. TSUNO, D. TSYBYCHEV, J.M. TUGGLE, M. TURALA, D. TURECEK, I. TURK CAKIR, E. TURLAY, P.M. TUTS, M.S. TWOMEY, M. TYLMAD, M. TYNDEL, D. TYPALDOS, H. TYRVAINEN, E. TZAMARIOUDAKI, G. TZANAKOS, K. UCHIDA, I. UEDA, M. UGLAND, M. UHLENBROCK, M. UHRMACHER, F. UKEGAWA, G. UNAL, D.G. UNDERWOOD, A. UNDRUS, G. UNEL, Y. UNNO, D. URBANIEC, E. URKOVSKY, P. URQUIJO, P. URREJOLA, G. USAI, M. USLENGHI, L. VACAVANT, V. VACEK, B. VACHON, S. VAHSEN, C. VALDERANIS, J. VALENTA, P. VALENTE, S. VALENTINETTI, S. VALKAR, E. VALLADOLID GALLEGO, S. VALLECORSA, J.A. VALLS FERRER, R. VAN BERG, H. VAN DER GRAAF, E. VAN DER KRAAIJ, E. VAN DER POEL, D. VAN DER STER, B. VAN EIJK, N. VAN ELDIK, P. VAN GEMMEREN, Z. VAN KESTEREN, I. VAN VULPEN, W. VANDELLI, G. VANDONI, A. VANIACHINE, P. VANKOV, F. VANNUCCI, F. VARELA RODRIGUEZ, R. VARI, E.W. VARNES, D. VAROUCHAS, A. VARTAPETIAN, K.E. VARVELL, L. VASILYEVA, V.I. VASSILAKOPOULOS, F. VAZEILLE, G. VEGNI, J.J. VEILLET, C. VELLIDIS, F. VELOSO, R. VENESS, S. VENEZIANO, A. VENTURA, D. VENTURA, S. VENTURA, M. VENTURI, N. VENTURI, V. VERCESI, M. VERDUCCI, W. VERKERKE, J.C. VERMEULEN, L. VERTOGARDOV, M.C. VETTERLI, I. VICHOU, T. VICKEY, G.H.A. VIEHHAUSER, M. VILLA, E.G. VILLANI, M. VILLAPLANA PEREZ, E. VILUCCHI, P. VINCENT, M.G. VINCTER, E. VINEK, V.B. VINOGRADOV, M. VIRCHAUX, S. VIRET, J. VIRZI, O. VITELLS, I. VIVARELLI, F. VIVES VAQUE, S. VLACHOS, M. VLASAK, N. VLASOV, A. VOGEL, H. VOGT, P. VOKAC, C.F. VOLLMER, M. VOLPI, G. VOLPINI, H. VON DER SCHMITT, J. VON LOEBEN, H. VON RADZIEWSKI, E. VON TOERNE, V. VOROBEL, A.P. VOROBIEV, V. VORWERK, M. VOS, K.C. VOSS, R. VOSS, T.T. VOSS, J.H. VOSSEBELD, A.S. VOVENKO, N. VRANJES, M. VRANJES MILOSAVLJEVIC, V. VRBA, M. VREESWIJK, T. VU ANH, B. VUARIDEL, D. VUDRAGOVIC, R. VUILLERMET, I. VUKOTIC, A. WAANANEN, P. WAGNER, H. WAHLEN, J. WALBERSLOH, J. WALDER, R. WALKER, W. WALKOWIAK, R. WALL, S. WALSH, C. WANG, H. WANG, J. WANG, J.C. WANG, M.W. WANG, S.M. WANG, F. WAPPLER, A. WARBURTON, C.P. WARD, M. WARSINSKY, R. WASTIE, P.M. WATKINS, A.T. WATSON, M.F. WATSON, G. WATTS, S. WATTS, A.T. WAUGH, B.M. WAUGH, M. WEBEL, G. WEBER, J. WEBER, M.D. WEBER, M. WEBER, M.S. WEBER, P. WEBER, A.R. WEIDBERG, J. WEINGARTEN, C. WEISER, H. WELLENSTEIN, H.P. WELLISCH, P.S. WELLS, M. WEN, T. WENAUS, S. WENDLER, T. WENGLER, S. WENIG, N. WERMES, M. WERNER, P. WERNER, M. WERTH, U. WERTHENBACH, M. WESSELS, K. WHALEN, S.J. WHEELER-ELLIS, S.P. WHITAKER, A. WHITE, M.J. WHITE, S. WHITE, S.R. WHITEHEAD, D. WHITESON, D. WHITTINGTON, F. WICEK, D. WICKE, F.J. WICKENS, W. WIEDENMANN, M. WIELERS, P. WIENEMANN, M. WIESMANN, C. WIGLESWORTH, L.A.M. WIIK, A. WILDAUER, M.A. WILDT, I. WILHELM, H.G. WILKENS, E. WILLIAMS, H.H. WILLIAMS, W. WILLIS, S. WILLOCQ, J.A. WILSON, M.G. WILSON, A. WILSON, I. WINGERTER-SEEZ, F. WINKLMEIER, M. WITTGEN, E. WOEHRLING, M.W. WOLTER, H. WOLTERS, B.K. WOSIEK, J. WOTSCHACK, M.J. WOUDSTRA, K. WRAIGHT, C. WRIGHT, D. WRIGHT, B. WRONA, S.L. WU, X. WU, J. WUESTENFELD, E. WULF, R. WUNSTORF, B.M. WYNNE, L. XAPLANTERIS, S. XELLA, S. XIE, Y. XIE, D. XU, G. XU, N. XU, M. YAMADA, A. YAMAMOTO, K. YAMAMOTO, S. YAMAMOTO, T. YAMAMURA, J. YAMAOKA, T. YAMAZAKI, Y. YAMAZAKI, Z. YAN, H. YANG, S. YANG, U.K. YANG, Y. YANG, Z. YANG, W.-M. YAO, Y. YAO, K. YARRADODDI, Y. YASU, J. YE, S. YE, M. YILMAZ, R. YOOSOOFMIYA, K. YORITA, H. YOSHIDA, R. YOSHIDA, C. YOUNG, S.P. YOUSSEF, D. YU, J. YU, J. YUAN, L. YUAN, A. YURKEWICZ, V.G. ZAETS, R. ZAIDAN, A.M. ZAITSEV, Z. ZAJACOVA, YO.K. ZALITE, V. ZAMBRANO, L. ZANELLO, P. ZARZHITSKY, A. ZAYTSEV, M. ZDRAZIL, C. ZEITNITZ, M. ZELLER, P.F. ZEMA, A. ZEMLA, C. ZENDLER, A.V. ZENIN, O. ZENIN, T. ZENIS, Z. ZENONOS, S. ZENZ, D. ZERWAS, G. ZEVI DELLA PORTA, Z. ZHAN, H. ZHANG, J. ZHANG, Q. ZHANG, X. ZHANG, L. ZHAO, T. ZHAO, Z. ZHAO, A. ZHEMCHUGOV, S. ZHENG, J. ZHONG, B. ZHOU, N. ZHOU, Y. ZHOU, C.G. ZHU, H. ZHU, Y. ZHU, X. ZHUANG, V. ZHURAVLOV, B. ZILKA, R. ZIMMERMANN, S. ZIMMERMANN, M. ZIOLKOWSKI, R. ZITOUN, L. ZIVKOVIC, V.V. ZMOUCHKO, G. ZOBERNIG, A. ZOCCOLI, Y. ZOLNIEROWSKI, A. ZSENEI, M. ZUR NEDDEN, and V. ZUTSHI
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Top quark ,Physics and Astronomy (miscellaneous) ,01 natural sciences ,High Energy Physics - Experiment ,High Energy Physics - Experiment (hep-ex) ,[PHYS.HEXP]Physics [physics]/High Energy Physics - Experiment [hep-ex] ,Invariant mass ,Detectors de radiació ,Physics ,ddc:539 ,Luminosity (scattering theory) ,Large Hadron Collider ,Settore FIS/01 - Fisica Sperimentale ,ATLAS ,medicine.anatomical_structure ,Physical Sciences ,ComputingMethodologies_DOCUMENTANDTEXTPROCESSING ,Física nuclear ,LHC ,Particle Physics - Experiment ,Particle physics ,Ciências Naturais::Ciências Físicas ,Ciências Físicas [Ciências Naturais] ,FOS: Physical sciences ,Astrophysics::Cosmology and Extragalactic Astrophysics ,ddc:500.2 ,charge asymmetry ,top quark pair production ,pp collisions ,ATLAS detector ,Computer Science::Digital Libraries ,Partícules (Física nuclear) ,Nuclear physics ,Atlas (anatomy) ,0103 physical sciences ,medicine ,Fysik ,ddc:530 ,High Energy Physics ,010306 general physics ,Engineering (miscellaneous) ,Ciencias Exactas ,top quark ,Astrophysics::Galaxy Astrophysics ,Science & Technology ,Muon ,010308 nuclear & particles physics ,Parton Distributions ,Física ,Pair production ,HADRON-HADRON COLLISIONS ,Col·lisions (Física nuclear) ,Experimental High Energy Physics ,proton-proton collisions ,High Energy Physics::Experiment ,Lepton - Abstract
A measurement of the top-antitop production charge asymmetry A[subscript C] is presented using data corresponding to an integrated luminosity of 1.04 fb[superscript −1] of pp collisions at s√=7 TeV collected by the ATLAS detector at the LHC. Events are selected with a single lepton (electron or muon), missing transverse momentum and at least four jets of which at least one jet is identified as coming from a b-quark. A kinematic fit is used to reconstruct the t[bar over t] event topology. After background subtraction, a Bayesian unfolding procedure is performed to correct for acceptance and detector effects. The measured value of A[subscript C] is A[subscript C]=−0.019±0.028 (stat.)±0.024 (syst.), consistent with the prediction from the MC@NLO Monte Carlo generator of A[subscript C]=0.006±0.002. Measurements of A[subscript C] in two ranges of invariant mass of the top-antitop pair are also shown., United States. Dept. of Energy, National Science Foundation (U.S.), Brookhaven National Laboratory
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- 2012
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4. Endoscopic surgery without decompressive craniectomy for large putaminal intracerebral hemorrhage: how I do it.
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Ye Y, Lan T, Xiao Y, and Yang C
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- Humans, Male, Middle Aged, Female, Aged, Neuroendoscopy methods, Treatment Outcome, Endoscopy methods, Cerebral Hemorrhage surgery, Cerebral Hemorrhage diagnostic imaging, Adult, Putaminal Hemorrhage surgery, Putaminal Hemorrhage diagnostic imaging, Decompressive Craniectomy methods
- Abstract
Background: Endoscopic hematoma evacuation is an efficient and secure minimally invasive procedure for intracerebral hemorrhages, characterized by a greater evacuation rate and reduced complications., Method: Pure endoscopic surgery without decompressive craniectomy was used to remove the clot in individuals with large putaminal intracerebral hemorrhage. The intracranial pressure was monitored after surgery., Conclusion: Endoscopic hematoma evacuation without decompressive craniectomy is safe and effective for patients with large putaminal intracerebral hemorrhage., Competing Interests: Declarations. Ethical approval: The investigation involving human subjects was examined and authorized by Liuzhou People's Hospital. The patients gave their written informed permission to be involved in this investigation. Competing interests: The authors declare no competing interests., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
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- 2024
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5. Boosting the catalytic activity of nanostructured ZnFe 2 O 4 spinels incorporating with Cu 2+ for photo-Fenton degradation under visible light.
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Nguyen LTT, Nguyen TT, Nguyen LTH, Mai TX, Bui ND, Chu NM, Nguyen HQ, Nguyen NTT, and Tran TV
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- Catalysis, Water Pollutants, Chemical chemistry, Ferric Compounds chemistry, Methylene Blue chemistry, Wastewater chemistry, Iron chemistry, Aluminum Oxide, Magnesium Oxide, Copper chemistry, Hydrogen Peroxide chemistry, Light, Nanostructures chemistry
- Abstract
Methylene blue (MB) is hazardous in natural water because this dye causes serious diseases that endangers public health and ecosystems. Photocatalytic degradation is a prominent technique for achieving the effective elimination of dye pollutants from wastewater and contribute vitally to ecology and environmental safety. Herein, Cu
2+ -substituted ZnFe2 O4 nanomaterials (Cux Zn1-x Fe2 O4 ; x = 0, 0.1, 0.2, 0.3, 0.4, 0.6) were synthesized, characterized, and applied for the photocatalytic degradation of MB dye beneath visible light with the assistance of hydrogen peroxide (H2 O2 ). The feature of the photo-catalysts was determined by XRD, EDX, FTIR, DRS, BET, SEM, and TEM techniques. Incorporation of Cu2+ ions changed the crystalline phase, particle size, morphology, and surface area. The photocatalysis condition was optimized with the following major factors, the amout of doping Cu2+ ions, H2 O2 concentration, adsorbent dosage, and MB concentration. As a result, the photocatalytic MB degradation efficiency by Cu0.6 Zn0.4 Fe2 O4 catalyst was 99.83% within 90 min under LED light (λ ≥ 420 nm), which was around 4 folds higher than that of pure ZnFe2 O4 . The photo-Fenton kinetics were in accordance with the pseudo-first-order kinetic model (R2 = 0.981), giving the highes rate constant of 0.034 min-1 . It can be, therefore, concluded that Cu2+ substitution considerably boosted the photocatalytic activity of Cux Zn1-x Fe2 O4 ZnFe2 O4 , suggesting a bright prospect of Cu0.6 Zn0.4 Fe2 O4 as a photo-catalyst in the dyes wastewater treatment., Competing Interests: Declarations. Ethics approval: Not applicable. Consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. Disclosure: The authors declare that all authors have been personally and actively involved in substantive work leading to the manuscript and will hold themselves jointly and individually responsible for its content., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)- Published
- 2024
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6. The Implementation of a MOOC-Based Flipped Classroom Teaching Method in the Context of Oncology Radiotherapy Residency Training.
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Yu DY, Zhang L, Li YL, Lan T, Gu LN, and Yang SS
- Abstract
The objective of this study is to examine the efficacy of the flipped classroom blended teaching method in the context of massive open online courses (MOOCs) for implementing standardized training and teaching of residents in oncology radiotherapy. A total of 48 junior residents who received standardized training at the Oncology Radiology Department of Harbin Medical University Cancer Hospital between September 2021 and August 2023 were randomly divided into two groups-i.e., the research group (24 cases) and the control group (24 cases)-using the random number table method. The control group received conventional didactic training, whereas the research group participated in a blended learning approach based on the MOOC model. The assessment results, along with the evaluations of teaching effectiveness, self-learning ability, and teaching satisfaction questionnaires, were observed and compared for the two groups of students. Compared with the control group, the research group presented significantly higher scores on theoretical foundations, skill operation, and case analysis (P < 0.05). The research group also showed greater outcomes than the control group in terms of improved theoretical knowledge, problem-solving skills, self-learning ability, teamwork, and communication (P < 0.05). The students in the research group presented significantly higher scores on measures of self-motivation beliefs, task analysis, self-monitoring and adjustment, and self-evaluation than those in the control group (P < 0.05). The research group also demonstrated significantly higher levels of satisfaction than the control group in terms of improvements in learning interest and initiative, clinical thinking ability, problem-solving ability, team cooperation ability, and the level of radiotherapy target delineation (P < 0.05). The implementation of MOOC-based flipped classroom blended teaching was shown to have positive effects on the standardized training and teaching of residents in the field of oncology radiotherapy. This approach can undoubtedly enhance students' academic performance, problem-solving abilities, and self-learning aptitudes while effectively stimulating their learning interests and initiative. Therefore, MOOC-based flipped classroom blended teaching is a valuable candidate for clinical application and promotion., (© 2024. The Author(s) under exclusive licence to American Association for Cancer Education.)
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- 2024
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7. Psychiatry Vice Chairs of Education: Expected and Actual Responsibilities, Satisfaction, and Work Barriers.
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Czelusta KT and Appelbaum NP
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- Humans, Leadership, Surveys and Questionnaires, Job Satisfaction, Faculty, Medical, Faculty, Psychiatry education
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Objectives: The current study investigated the gap between actual and expected work responsibilities of psychiatry vice chairs of education (VCEs), their work satisfaction, and perceived work barriers., Methods: Psychiatry VCEs were identified through professional memberships and school websites within the USA. In 2021, identified VCEs were emailed an anonymous survey that was based on past research on roles and responsibilities of VCEs. Through qualitative and quantitative questions, the survey assessed demographic characteristics, actual and expected work responsibilities, work satisfaction, and work barriers during time spent in the VCE role., Results: Of 57 identified psychiatry VCEs, 32 (56.1%) completed the survey. Most respondents (59.4%) said their department/institution did not have a written VCE job description. There were gaps between actual and expected VCE responsibilities, especially with the performance review of education leaders (Δ - 11; actual n = 20 vs. expected n = 31). The greatest satisfaction came from mentoring trainees/faculty (respectively 4.88 ± 0.33 and 4.85 ± 0.37 on a 5-point scale) and overseeing/supporting educational programs (4.66 ± 0.48). Comments on work barriers included limited protected time (n = 11), education budget constraints (n = 6), and having multiple leadership roles (n = 5). The actual full-time equivalent in the VCE role was significantly lower than expected (p < .001)., Conclusion: Considering the role of psychiatry VCEs remains relatively new and is evolving, adequate allocation of resources and improved job descriptions may help close the gap between expected and actual responsibilities. The current findings can assist in developing such job descriptions to attract and define the work of a VCE based on specific responsibilities and associated work satisfaction., (© 2024. The Author(s), under exclusive licence to Academic Psychiatry, LLC.)
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- 2024
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8. Integration of transcriptome and metabolome analyses reveals the role of OsSPL10 in rice defense against brown planthopper.
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Lu L, Sun Z, Wang R, Du Y, Zhang Z, Lan T, Song Y, and Zeng R
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- Animals, Transcriptome, Gene Expression Regulation, Metabolome, Gene Expression Regulation, Plant, Oryza genetics, Oryza metabolism, Hemiptera physiology
- Abstract
Key Message: OsSPL10 is a negative regulator of rice defense against BPH, knockout of OsSPL10 enhances BPH resistance through upregulation of defense-related genes and accumulation of secondary metabolites. Rice (Oryza sativa L.), one of the most important staple foods worldwide, is frequently attacked by various herbivores, including brown planthopper (BPH, Nilaparvata lugens). BPH is a typical monophagous, phloem-sucking herbivore that has been a substantial threat to rice production and global food security. Understanding the regulatory mechanism of defense responses to BPH is essential for improving BPH resistance in rice. In this study, a SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE 10 (OsSPL10) transcription factor was found to play a negative role in the defenses of rice against BPH. To gain insights into the molecular and biochemical mechanisms of OsSPL10, we performed combined analyses of transcriptome and metabolome, and revealed that knockout of OsSPL10 gene improved rice resistance against BPH by enhancing the direct and indirect defenses. Genes involved in plant hormone signal transduction, MAPK signaling pathway, phenylpropanoid biosynthesis, and plant-pathogen interaction pathway were significantly upregulated in spl10 mutant. Moreover, spl10 mutant exhibited increased accumulation of defense-related secondary metabolites in the phenylpropanoid and terpenoid pathways. Our findings reveal a novel role for OsSPL10 gene in regulating the rice defense responses, which can be used as a potential target for genetic improvement of BPH resistance in rice., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
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- 2023
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9. Biological and chemical nitrification inhibitors exhibited different effects on soil gross N nitrification rate and N 2 O production: a 15 N microcosm study.
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Lan T, Chen X, Liu S, Zhou M, and Gao X
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- Nitrification, Ammonia analysis, Dimethylphenylpiperazinium Iodide pharmacology, Soil Microbiology, Archaea, Phosphates pharmacology, Oxidation-Reduction, Soil chemistry, Betaproteobacteria
- Abstract
Nitrification inhibitors (NIs) are considered as an effective strategy for reducing nitrification rate and related environmental nitrogen (N) loss. However, whether plant-derived biological NIs had an advantage over chemical NIs in simultaneously inhibiting nitrification rate and N
2 O production remains unclear. Here, we conducted an aerobic15 N microcosmic incubation experiment to compare the effects of a biological NI (methyl 3-(4-hydroxyphenyl) propionate, MHPP) with three chemical NIs, 2-chloro-6-(trichloromethyl) pyridine (nitrapyrin), dicyandiamide (DCD), and 3,4-dimethylpyrazole phosphate (DMPP) on (i) gross N mineralization and nitrification rate and (ii) the relative importance of nitrification and denitrification in N2 O emission in a calcareous soil. The results showed that DMPP significantly inhibited m_gross rate (P < 0.05), whereas DCD, nitrapyrin, and MHPP only numerically inhibited it. Gross N nitrification (n_gross ) rates were inhibited by 9.48% in the DCD treatment to 51.5% in the nitrapyrin treatment. Chemical NIs primarily affected the amoA gene abundance of ammonia-oxidizing bacteria (AOB), whereas biological NIs affected the amoA gene abundance of ammonia-oxidizing archaea (AOA) and AOB. AOB's community composition was more susceptible to NIs than AOA, and NIs mainly targeted Nitrosospira clusters of AOB. Chemical NIs of DCD, DMPP, and nitrapyrin proportionally reduced N2 O production from nitrification and denitrification. However, the biological NI MHPP stimulated short-term N2 O emission and increased the proportion of N2 O from denitrification. Our findings showed that the influence of NIs on gross N mineralization rate (m_gross ) was dependent on the NI type. MHPP exhibited a moderate n_gross inhibitory capacity compared with the three chemical NIs. The mechanisms of chemical and biological NIs inhibiting n_gross can be partly attributed to changes in the abundance and community of ammonia oxidizers. A more comprehensive evaluation is needed to determine whether biological NIs have advantages over chemical NIs in inhibiting greenhouse gas emissions., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)- Published
- 2023
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10. Essential oil extracted from Quzhou Aurantii Fructus prevents acute liver failure through inhibiting lipopolysaccharide-mediated inflammatory response.
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Lan T, Wang W, Huang DL, Zeng XX, Wang XX, Wang J, Tong YH, Mao ZJ, and Wang SW
- Abstract
Quzhou Aurantii Fructus (QAF) has a long history as a folk medicine and food for the treatment of liver diseases. While our earlier study provided evidence of hepatoprotective properties contained within the flavonoids and limonins constituents in QAF, the potential preventative effects afforded by essential oil components present within QAF remains enigmatic. In this study, we prepared Quzhou Aurantii Fructus essential oil (QAFEO) and confirmed its anti-inflammatory effects on liver inflammation through experimentation on lipopolysaccharide and D-galactosamine (LPS/D-GalN) induced acute liver failure (ALF) mouse models. Using RNA-sequence (RNA-seq) analysis, we found that QAFEO prevented ALF by systematically blunting the pathways involved in response to LPS and toll-like receptor signaling pathways. QAFEO effectively suppressed the phosphorylation of tank-binding kinase 1 (TBK1), TGF-beta activated kinase 1 (TAK1), interferon regulatory factor 3 (IRF3), and the activation of mitogen activated kinase-like protein (MAPK) and nuclear factor-kappa B (NF-κB) pathways in vivo and in vitro. Importantly, QAFEO substantially reduced myeloid differentiation primary response gene 88 (MyD88)- toll-like receptor 4 (TLR4) interaction levels. Moreover, 8 compounds from QAFEO could directly bind to REAL, TAK1, MyD88, TBK1, and IRF3. Taken together, the results of our study support the notion that QAFEO exerts a hepatoprotective effect through inhibiting LPS-mediated inflammatory response., (© 2023. Kunming Institute of Botany, CAS.)
- Published
- 2023
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11. Fabrication of sodium alginate-doped carbon dot composite hydrogel and its application for La (III) adsorption and enhanced the removal of phosphorus.
- Author
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Zhang X, Luo S, Duan J, Lan T, and Wei Y
- Subjects
- Hydrogels chemistry, Carbon, Phosphorus, Wastewater, Adsorption, Alginates chemistry, Kinetics, Hydrogen-Ion Concentration, Lanthanoid Series Elements, Water Pollutants, Chemical analysis
- Abstract
Adsorption is an effective method for the removal of hazardous substances from wastewater. In this work, a low-cost and environmental-friendly composite hydrogel material of sodium alginate doped with nitrogen doped carbon dots (SA@NCDs) was fabricated by impregnation for lanthanide and enhanced phosphorus adsorption in wastewater. The effects of NCDs doping amount, dosage, pH, initial solution concentration, adsorption time and temperature on the process of La (III) adsorption by SA@NCDs were investigated. The adsorption isotherms fitted to Langmuir isotherm model (R
2 = 0.9970-0.9989) and the adsorption kinetics followed pseudo-second-order kinetic model (R2 = 0.9992). The maximum adsorption capacity of the adsorbent for La (III) was 217.39 mg/g according to the Langmuir model at 298.15 K. After five cycles, the removal efficiency of La (III) adsorbed by SA@NCDs was still 85.1%. Moreover, the loaded La (III) enhanced the adsorption of phosphorus. The La (III)-SA@NCDs-5 hydrogel adsorbent greatly improved the adsorption capacity for phosphorus compared with the La (III)-free adsorbent, and the adsorption amount can reach 9.64 mg-P/g. The SA@NCDs complex hydrogels for rare earth adsorption were prepared by introducing NCDs rich in amino group into SA hydrogels. The introduction of NCDs increases the adsorption sites of hydrogels, and also overcomes the problem that NCDs itself is difficult to recover in wastewater treatment applications. The lanthanide adsorbed material has a stable structure and can be used to remove phosphorus to deal with waste using the waste. It indicates the SA@NCDs hydrogel composite adsorbent have good potential for wastewater treatment., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)- Published
- 2023
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12. Nitrogen removal characteristics of heterotrophic nitrification-aerobic denitrification bacterium Acinetobacter ZQ-A1 and community characteristics analysis of its application in pig farm wastewater.
- Author
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Cao Z, Huang F, Zhang R, Zhao X, Wang Y, Wu Y, Liao X, Feng Y, Ma J, and Lan T
- Subjects
- Animals, Swine, Wastewater, Denitrification, Sewage microbiology, Nitrogen metabolism, Farms, Aerobiosis, Bacteria metabolism, Heterotrophic Processes, Nitrites metabolism, Nitrification, Acinetobacter metabolism
- Abstract
A heterotrophic nitrifying aerobic denitrifying (HN-AD) strain ZQ-A1 with excellent denitrification performance, identified as Acinetobacter, was isolated from simultaneous nitrification and denitrification (SND) craft. ZQ-A1 was capable of removing NH
4 + , NO2 - , and NO3 - ; the 21-hour removal rates were 84.84%, 87.13%, and 92.63%. ZQ-A1 has the ability to treat mixed nitrogen sources. In addition, ZQ-A1 can be well applied to actual sewage. According to the analysis of microbial community characteristics, the relative abundance of Acinetobacter in the experimental group increased from 0.06% to 2.38%, which is an important reason for the removal rate of NH4 + exceeding 99% within 30 days. The results of KEGG function prediction showed that with the addition of ZQ-A1, the relative abundance of pathways related to bacterial metabolism, such as tricarboxylic acid cycle metabolism, was higher. The research expanded the thinking of HN-AD bacteria in actual production and laid a foundation for its application in sewage treatment., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)- Published
- 2023
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13. YEATS2 regulates the activation of TAK1/NF-κB pathway and is critical for pancreatic ductal adenocarcinoma cell survival.
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Sheng H, Zheng F, Lan T, Chen HF, Xu CY, Wang SW, Weng YY, Xu LF, and Zhang F
- Subjects
- Humans, Cell Line, Tumor, Cell Survival, NF-kappa B metabolism, Carcinoma, Pancreatic Ductal drug therapy, Pancreatic Neoplasms pathology
- Abstract
The prognosis of pancreatic ductal adenocarcinoma (PDAC) is poor despite diagnostic progress and new chemotherapeutic regimens. Constitutive activation of NF-κB is frequently observed in PDAC. In this study, we found that YEATS2, a scaffolding protein of ATAC complex, was highly expressed in human PDAC. Depletion of YEATS2 reduced the growth, survival, and tumorigenesis of PDAC cells. The binding of YEATS2 is crucial for maintaining TAK1 activation and NF-κB transcriptional activity. Of importance, our results reveal that YEATS2 promotes NF-κB transcriptional activity through modulating TAK1 abundance and directly interacting with NF-κB as a co-transcriptional factor., (© 2021. The Author(s), under exclusive licence to Springer Nature B.V.)
- Published
- 2023
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14. Enantioselective hydrolysis and photolysis of mandipropamid in different aquatic environments - evaluation of influencing factors.
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Li J, Han J, Lan T, Mu S, Hu D, and Zhang K
- Subjects
- Amides, Carboxylic Acids, Chromatography, High Pressure Liquid, Hydrolysis, Photolysis, Stereoisomerism, Tandem Mass Spectrometry, Water chemistry, Ecosystem, Water Pollutants, Chemical analysis
- Abstract
The hydrolysis and photolysis of the chiral fungicide mandipropamid were investigated, and the potential enantioselectivity of mandipropamid in solutions was further assessed. The aqueous solutions were filtered and directly injected into the liquid chromatography with tandem mass spectrometry. In the hydrolysis experiments, mandipropamid enantiomers hydrolyzed slowly in aquatic solutions with half-lives > 200 days; nevertheless, rise of the pH and incubation temperature could increase the hydrolysis rates more than 1.1 times (half-lives decreased from 495.1 to 216.6 days). Compared with the hydrolysis results, photolysis was found to be the main degradation pathway for mandipropamid in different solutions (half-lives < 14 h, except in pH = 5.05 buffer solution). Organic solvents were able to accelerate the photolysis of mandipropamid, but acidic solutions and the addition of flavonoids or inorganic salts significantly inhibited the photolysis of mandipropamid. During the hydrolysis and photolysis processes, the configuration of mandipropamid enantiomers was stable and five possible transformation products were identified by high resolution mass spectrometry. Due to the enantiomeric fraction values > 0.5, the hydrolysis and photolysis of mandipropamid were enantioselective, and S-( +)-mandipropamid preferentially disspated in certain aqueous solutions. The systematic evaluation of the hydrolysis and photolysis of mandipropamid enantiomers may provide more accurate data for better assessment of environmental and ecological risks in aquatic ecosystems., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
- Published
- 2022
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15. Crystalline silica induces macrophage necrosis and causes subsequent acute pulmonary neutrophilic inflammation.
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Nie W, Lan T, Yuan X, Luo M, Shen G, Yu J, and Wei X
- Subjects
- Cathepsin B metabolism, DNA, Mitochondrial metabolism, Humans, Inflammation metabolism, Macrophages metabolism, Necrosis chemically induced, Necrosis metabolism, Pneumonia chemically induced, Silicon Dioxide toxicity
- Abstract
Crystalline silica (CS), an airborne particulate, is a major global occupational health hazard. While it is known as an important pathogenic factor in many severe lung diseases, the underlying mechanisms of its toxicity are still unclear. In the present study, we found that intra-tracheal instillation of CS caused rapid emergence of necrotic alveolar macrophages. Cell necrosis was a consequence of the release of cathepsin B in CS-treated macrophages, which caused dysfunction of the mitochondrial membrane. Damage to mitochondria disrupted Na
+ /K+ ATPase activity in macrophages, leading to intracellular sodium overload and the subsequent cell necrosis. Further studies indicate that CS-induced macrophage necrosis and the subsequent release of mitochondrial DNA could trigger the recruitment of neutrophils in the lung, which was regulated by the TLR9 signaling pathway. In conclusion, our results suggest a novel mechanism whereby CS leads to rapid macrophage necrosis through cathepsin B release, following the leakage of mitochondrial DNA as a key event in the induction of pulmonary neutrophilic inflammation. This study has important implications for the early prevention and treatment of diseases induced by CS., (© 2021. The Author(s), under exclusive licence to Springer Nature B.V.)- Published
- 2022
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16. Establishment of Detection Methods for Five Cannabinoids in Hemp Cosmetics Based on HPLC.
- Author
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Sun DM, Song L, Wang HY, Yu CC, Chu Q, Lan T, and Zhang WB
- Subjects
- Chromatography, High Pressure Liquid, Cannabinoids analysis, Cannabis, Cosmetics
- Abstract
A simultaneously HPLC detection method for cannabidiolic acid (CBDA), cannabidiol (CBD), cannabinol (CBN), Δ9-tetrahydrocannabinol (THC), tetrahydro-cannabinolic acid (THCA) in 3 kinds of cosmetics matrix containing hemp leaf extract was developed. The extraction and HPLC conditions were optimized, and a methodological verification was also carried out. The results showed that this method had a good linear relationship in the range of 0.25 - 50 μg/mL with LOD values for 5 cannabinoids all between 0.10 - 0.25 μg/g. The recovery rates of 5 cannabinoids in 3 different cosmetics matrixes were between 90.1 - 108.5%, and the RSD values were all below 4.4%. These results indicated that this method had the advantages of simple operation, high sensitivity, and good accuracy. Through the testing of 6 kinds of hemp cosmetics, it was found that such cosmetics had uneven quality. The establishment of this method can lay a methodological foundation for establishing relevant testing method standards.
- Published
- 2021
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17. Regulating CH 4 , N 2 O, and NO emissions from an alkaline paddy field under rice-wheat rotation with controlled release N fertilizer.
- Author
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Lan T, Zhang H, Han Y, Deng O, Tang X, Luo L, Zeng J, Chen G, Wang C, and Gao X
- Subjects
- Agriculture, Delayed-Action Preparations, Global Warming, Methane, Nitrous Oxide analysis, Rotation, Soil, Triticum, Fertilizers analysis, Oryza
- Abstract
Controlled release fertilizer (CRF) has been shown to increase crop yield and N use efficiency (NUE) compared with traditional chemical fertilizer (TF). However, few studies examined the effects of CRF on CH
4 , N2 O, and NO emissions simultaneously in alkaline paddy fields under rice-wheat rotation. In the present study, we conducted a 2-year field experiment to compare the effects of different CRF application strategies on these gas emissions with those of TF and explored the effects of CRF on global warming potential (GWP), crop yields, and greenhouse gas emission intensity (GHGI). Results showed that CRF can reduce 0.98-14.3%, 13.3-21.1%, and 8.22-16.3% of CH4 , N2 O, and NO emissions, respectively, in the studied alkaline paddy field. CRF reduce CH4 emission probably by regulating soil NH4 + concentration. CRF reduce N2 O and NO emissions probably by regulating inorganic N content in the studied alkaline paddy soil. CRF had the same effect on annual crop yield as TF, especially when CRF was applied twice in each season and had the same N application rate as TF. Annual crop yields and the agronomic efficiency of N (AEN ) increased by 8.24% and 21.6%, respectively. On the average of the two rice-wheat rotation cycles, GHGI significantly decreased by up to 14.1% after the application of CRF as relative to that after the application of TF (P < 0.05). These results suggest that CRF is an environment-friendly N fertilization strategy for mitigating GWP and ensuring high crop yield in an alkaline paddy field under rice-wheat rotation.- Published
- 2021
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18. The near-infrared fluorescent dye IR-780 was coupled with cabazitaxel for castration-resistant prostate cancer imaging and therapy.
- Author
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Zheng Y, Hou G, Zhang G, Lan T, Yuan J, Zhang L, Yan F, Wang F, Meng P, Dun X, Li X, Chen G, Zhu Z, Wei D, He W, and Yuan J
- Subjects
- Animals, Antineoplastic Agents chemistry, Apoptosis drug effects, Cell Cycle drug effects, Cell Line, Cell Movement drug effects, Cell Survival drug effects, Fluorescent Dyes chemistry, Humans, Indoles chemistry, Male, Mice, Nude, Taxoids chemistry, Antineoplastic Agents administration & dosage, Fluorescent Dyes administration & dosage, Indoles administration & dosage, Prostatic Neoplasms, Castration-Resistant diagnostic imaging, Prostatic Neoplasms, Castration-Resistant drug therapy, Taxoids administration & dosage
- Abstract
A new drug, Caba-780, was synthesized by chemical coupling of the heptamethyl phthalocyanine near-infrared fluorescent (NIRF) dye IR-780 and the paclitaxel-based chemotherapeutic drug cabazitaxel. Then, the potential value of Caba-780 in the diagnosis and treatment of castration-resistant prostate cancer (CRPC) was evaluated. The CRPC cell lines DU145 and PC-3, as well as the normal human prostate stromal cell line WPMY-1, were used to evaluate the uptake of Caba-780 and its antitumor effect in vitro. The distribution, antitumor effect, and safety of Caba-780 were also evaluated in tumor-bearing mouse xenograft models. Our results showed that Caba-780 was efficiently absorbed by DU145 and PC-3 cells and that the cytotoxicity of Caba-780 was significantly stronger than that of IR-780 and cabazitaxel. In addition, Caba-780 inhibited the migration and invasion of DU145 and PC-3 cells and promoted apoptosis by prolonging the G2 phase of the cell cycle. Further analysis indicated that Caba-780 could be used to effectively image tumor xenografts. At the same time, this drug inhibited the growth of tumors in vivo. Therefore, the new synthetic drug Caba-780 has potential applications in the diagnosis and treatment of CRPC.
- Published
- 2020
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19. Development of a model for identification of individuals with high risk of osteoporosis.
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Ho-Pham LT, Doan MC, Van LH, and Nguyen TV
- Subjects
- Absorptiometry, Photon, Aged, Bone Density, Female, Femur Neck, Humans, Lumbar Vertebrae diagnostic imaging, Male, Middle Aged, Models, Theoretical, Risk Assessment, Osteoporosis diagnostic imaging, Osteoporosis epidemiology
- Abstract
Many developing countries, including Vietnam, lack DXA resources for the diagnosis of osteoporosis, which poses difficulties in the treatment and prevention of osteoporosis at the individual level. We have developed and validated a prediction model for individualized assessment of osteoporosis based on age and body weight for men and women., Purpose: To estimate the prevalence of osteoporosis and to develop and validate a prediction model for estimating the absolute risk of osteoporosis in the Vietnamese population., Methods: The study involved 1477 women and 669 men aged 50 years and older, who were recruited from the general population in Ho Chi Minh City (Vietnam). Bone mineral density (BMD) at the femoral neck, total hip, and lumbar spine was measured by DXA (Hologic Horizon). The diagnosis of osteoporosis was based on BMD T-score (T-score ≤ - 2.5) at the femoral neck or lumbar spine which was derived from a published reference range for the Vietnamese population. The logistic regression model was used to develop the prediction model for men and women separately. The bootstrap method was used to evaluate the model performance using 3 indices: the area under the receiver's operating characteristic curve (AUC), Brier score, and R-squared values., Results: The prevalence of osteoporosis at any site was 28.3% in women and 15.5% in men. The best predictors of osteoporosis risk were age and body weight. Using these indices, a cut-off of 0.195 for women yielded an AUC of 0.825, Brier score = 0.112, and it explained 33.8% of total variance in risk of osteoporosis between individuals. Similarly, in men, the internal validation with a cut-off of 0.09 yielded good accuracy, with AUC = 0.858, Brier score = 0.040, and R-squared = 30.3%., Conclusion: We have developed and validated a prediction model for individualized assessment of osteoporosis. In settings without DXA, this model can serve as a useful screening tool to identify high-risk individuals for DXA scan.
- Published
- 2020
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20. Metabolomic abnormalities of purine and lipids implicated olfactory bulb dysfunction of CUMS depressive rats.
- Author
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He Y, Wang Y, Wu Z, Lan T, Tian Y, Chen X, Li Y, Dang R, Bai M, Cheng K, and Xie P
- Subjects
- Animals, Disease Models, Animal, Male, Metabolomics, Rats, Rats, Sprague-Dawley, Depression metabolism, Depressive Disorder, Major metabolism, Lipid Metabolism physiology, Olfactory Bulb metabolism, Purines metabolism, Stress, Psychological metabolism
- Abstract
Major depressive disorder (MDD) is a serious mood disorder and leads to a high suicide rate as well as financial burden. The volume and function (the sensitivity and neurogenesis) of the olfactory bulb (OB) were reported to be altered among the MDD patients and rodent models of depression. In addition, the olfactory epithelium was newly reported to decrease its volume and function under chronic unpredictable mild stress (CUMS) treatment. However, the underlying molecular mechanism still remains unclear. Herein, we conducted the non-targeted metabolomics method based on gas chromatography-mass spectrometry (GC-MS) coupled with multivariate statistical analysis to characterize the differential metabolites in OB of CUMS rats. Our results showed that 19 metabolites were categorized into two perturbed pathways: purine metabolism and lipid metabolism, which were regarded as the vital pathways concerned with dysfunction of OB. These findings indicated that the turbulence of metabolic pathways may be partly responsible for the dysfunction of OB in MDD.
- Published
- 2020
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21. Efficient adsorption of Cd 2+ from aqueous solution using metakaolin geopolymers.
- Author
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Lan T, Li P, Rehman FU, Li X, Yang W, and Guo S
- Subjects
- Adsorption, Kinetics, Silicon Dioxide, Spectrometry, X-Ray Emission, Spectroscopy, Fourier Transform Infrared, Temperature, Wastewater chemistry, Water analysis, X-Ray Diffraction, Cadmium analysis, Water Pollutants, Chemical analysis
- Abstract
In this study, geopolymers were prepared using metakaolin (MK) raw material under different alkali activator moduli (SiO
2 /Na2 O = 0.8, 1.2, 1.6, 2.0 M ratio) in order to analyze their capacity and mechanism for adsorbing cadmium (Cd2+ ) from solution. Instrumental analyses including X-ray diffraction (XRD), scanning electron microscopy (SEM) with energy dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy(XPS), Fourier transform infrared (FTIR), and Brunauer-Emmett-Teller (BET) were performed to examine the mineralogical features of the MK and geopolymers before and after Cd2+ adsorption. The effect of initial pH, temperature, contact time, and initial concentration on Cd adsorption performance was studied to obtain the equilibrium isotherm. Kinetic data of the geopolymers fitted the pseudo-second-order kinetic model well. Moreover, the adsorption equilibrium data of Cd2+ adsorbed by the geopolymers fitted the Langmuir model better than the Freundlich model. The result shows that chemisorption dominates Cd2+ adsorption by geopolymers and that the adsorption capacity differs when prepared using different alkali-activated modulus agents. The geopolymer prepared using an alkali activator modulus of 0.8 M (molar ratio) exhibited the best Cd2+ adsorption performance with a maximum adsorption capacity of 70.3 mg g-1 . The removal rate of Cd2+ by geopolymer still remained above 85% after five round of recycling.- Published
- 2019
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22. Emission of nitrous oxide from plain multi-ditch system and its impact factors.
- Author
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Deng O, Li X, Xiao Y, Zhang S, Deng L, Lan T, Luo L, Gao X, Zhou W, Zhang J, and Ling J
- Subjects
- China, Environmental Monitoring, Fertilizers analysis, Nitrogen analysis, Seasons, Air Pollutants analysis, Drainage, Sanitary, Fresh Water chemistry, Groundwater chemistry, Nitrous Oxide analysis, Water Pollutants, Chemical analysis
- Abstract
Multi-level ditch area is a major component of the hydrographic net of plain area, China. Given the high concentration of nitrogen (N) in the surface water and vigorous biogeochemical interactions, ditch is likely to be the hot spots of N
2 O emission. However, N2 O emission flux and emission factor (EF5r ) of multi-level ditches have not been determined. To address this knowledge gap, a 1-year field work in three ditches with different levels in Chengdu Plain was conducted. It is found that the annual flux of N2 O emission and EF5r was higher in the lateral (0.0020 and 83.94 μg m-2 h-1 ) and field ditches (0.0019 and 110.75 μg m-2 h-1 ) than in the branch ditch (0.0016 and 46.38 μg m-2 h-1 , P < 0.05). It is found that parameters of groundwater level, discharge, precipitation, and NH4 + were the primary factors, and these parameters can model the N2 O flux well. Furthermore, the content of NH4 + in the surface water of ditches presented better correlation with the emission of N2 O than the content of NO3 - . Therefore, controlling NH4 + emission and lessening fertilizer usage in summer may be key solutions for indirect reduction of N2 O in Chengdu Plain.- Published
- 2019
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23. The distribution pattern of endopolyploidy in maize.
- Author
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Li S, Liu L, Li T, Lan T, Wang Y, Zhang Z, Liu J, Xu S, Zhang X, Zhu J, Xue J, and Guo D
- Subjects
- Flow Cytometry, Plant Development genetics, Zea mays growth & development, Polyploidy, Zea mays genetics
- Abstract
Key Message: We discovered that endopolyploidization is common in various organs and tissues of maize at different development stages. Endopolyploidy is not specific in maize germplasm populations. Endopolyploidy is caused by DNA endoreplication, a special type of mitosis with normal DNA synthesis and a lack of cell division; it is a common phenomenon and plays an important role in plant development. To systematically study the distribution pattern of endopolyploidy in maize, flow cytometry was used to determine the ploidy by measuring the cycle (C) value in various organs at different developmental stages, in embryos and endosperm during grain development, in roots under stress conditions, and in the roots of 119 inbred lines from two heterotic groups, Shaan A and Shaan B. Endopolyploidy was observed in most organs at various developmental stages except in expanded leaves and filaments. The endosperm showed the highest C value among all organs. During tissue development, the ploidy increased in all organs except the leaves. In addition, the endopolyploidization of the roots was significantly affected by drought stress. Multiple comparisons of the C values of seven subgroups revealed that the distribution of endopolyploidization was not correlated with the population structure. A correlation analysis at the seedling stage showed a positive relationship between the C value and both the length of the whole plant and the length of main root. A genome-wide association study (GWAS) identified a total of 9 significant SNPs associated with endopolyploidy (C value) in maize, and 8 candidate genes that participate in cell cycle regulation and DNA replication were uncovered in 119 maize inbred lines.
- Published
- 2019
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24. Enhanced of antibacterial activity of antibiotic-functionalized silver nanocomposites with good biocompatibility.
- Author
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Guo Q, Lan T, Chen Y, Xu Y, Peng J, Tao L, and Shen X
- Subjects
- Animals, Biocompatible Materials chemistry, Carbohydrates chemistry, Cell Survival, Escherichia coli drug effects, Graphite, Human Umbilical Vein Endothelial Cells, Humans, Mice, Microbial Sensitivity Tests, NIH 3T3 Cells, Polyglycolic Acid chemistry, Staphylococcus aureus drug effects, Tobramycin chemistry, Anti-Bacterial Agents chemistry, Drug Resistance, Bacterial, Metal Nanoparticles chemistry, Nanocomposites chemistry, Polyglycolic Acid analogs & derivatives, Silver chemistry
- Abstract
Antimicrobial resistance to traditional antibiotics leads to a serious concern for medical care owing to ineffective antibiotic therapies. This study focused on the preparation of silver nanocomposites (AgNPs@Tob&PAGA) by modifying AgNPs with tobramycin (Tob) and carbohydrate polymer of poly(2-(acrylamido) glucopyranose) (PAGA). The enhanced antibacterial activities of nanocomposites against common pathogens of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were explored. The introduction of PAGA onto silver nanocomposites improved both citocompatibility and antibacterial activity. Compared with nude Tob, AgNPs@Tob&PAGA showed more fascinating antimicrobial effect against E. coli and S. aureus with about 20-fold increase in the antibacterial activity, simultaneously no detectable resistance was observed. Consequently, the silver nanocomposite as an antimicrobial agent presents promising prospects in the treatment of bacterial infections caused by antimicrobial resistant bacteria.
- Published
- 2019
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25. The relationship between extreme temperature and emergency incidences: a time series analysis in Shenzhen, China.
- Author
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Guo Y, Ma Y, Ji J, Liu N, Zhou G, Fang D, Huang G, Lan T, Peng C, and Yu S
- Subjects
- Acute Disease epidemiology, Adult, Aged, Air Pollutants analysis, Air Pollution statistics & numerical data, Cardiovascular Diseases epidemiology, China epidemiology, Cities, Cold Temperature, Extreme Heat adverse effects, Female, Humans, Incidence, Male, Middle Aged, Nonlinear Dynamics, Risk, Seasons, Air Pollution adverse effects, Emergency Service, Hospital statistics & numerical data
- Abstract
Extreme temperature has been reported to be associated with an increase in acute disease incidence in several cities. However, few similar studies were carried out in Shenzhen, which is a subtropical city located in the southern China. This study explored the relationship between the emergency incidences and extreme temperatures, and investigated the role of air pollutants played in the temperature-related effects on human health in Shenzhen. We conducted a distributed lag nonlinear model study on the effect of extreme temperatures on emergency incidences in Shenzhen city during 2013-2017. Here, only the total emergency incidences, emergency incidences for respiratory diseases, and cardiovascular diseases were taken into consideration. Air pollution, subgroups, and seasons were adjusted to investigate the impacts of extreme temperatures on emergency incidences. Relative risk (RR) and 95% confidence intervals were calculated with the R software. From lag 0 to 21 days, the RR of temperature-total emergency department visits, temperature-cardiovascular, and temperature-respiratory diseases was 1.09 (95% CI: 0.98-1.20), 1.22 (95% CI: 0.96-1.56), and 1.06 (95% CI: 0.70-1.60) at extremely low temperature (first percent of temperature, 10 °C), respectively. During the same lag days, the RR was 1.02 (95 % CI: 0.92-1.14), 0.64 (95% CI: 0.49-0.86), and 0.92 (95% CI: 0.56-1.53) between extremely high temperature and total emergency department visits, cardiovascular, and respiratory diseases, respectively. The cumulative effects gradually went up with time for all types of emergency incidences in warm seasons (5 days moving average of temperature < 22 °C). However, the cumulative effects of total emergency incidences and Cvd emergency incidences were increased within the first lag 5 days, and then decreased until lag 21 in hot seasons (5 days moving average of temperature ≥ 22 °C). The cumulative effects of Res emergency incidences showed a declined trend from lag 0 to lag 21. The elderly (≥ 65, P1: RR = 1.49, 95% CI (1.30, 1.71); P99: RR = 0.86, 95% CI (0.71, 1.04)) and men (P1: RR = 1.27, 95% CI (1.14, 1.42)) seemed to be more vulnerable to extreme temperature than the younger (≤ 64, P1: RR = 1.19, 95% CI (1.08, 1.32); P99: RR = 1.00, 95% CI (0.89, 1.12)) and women (P1: RR = 1.17, 95%CI (1.06, 1.30)). The effects of extremely low temperature on all types of emergency incidences were stronger than those of extremely high temperature in the whole year. In addition, impacts of cold weather lasted about several days while those of hot weather were acute and rapid. An increased frequency of emergency incidences is predicted by rising temperatures variations. These results have clinical and public health implications for the management of emergency incidences.
- Published
- 2018
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26. Engineering CRISPR/Cpf1 with tRNA promotes genome editing capability in mammalian systems.
- Author
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Wu H, Liu Q, Shi H, Xie J, Zhang Q, Ouyang Z, Li N, Yang Y, Liu Z, Zhao Y, Lai C, Ruan D, Peng J, Ge W, Chen F, Fan N, Jin Q, Liang Y, Lan T, Yang X, Wang X, Lei Z, Doevendans PA, Sluijter JPG, Wang K, Li X, and Lai L
- Subjects
- Animals, Animals, Genetically Modified, Animals, Newborn, Bacterial Proteins metabolism, Cells, Cultured, Embryo, Mammalian, Endonucleases metabolism, Female, Fetus, Genome genetics, HEK293 Cells, HeLa Cells, Humans, Male, Mammals embryology, Mammals genetics, Mutagenesis, Nuclear Transfer Techniques, Pregnancy, RNA, Guide, CRISPR-Cas Systems genetics, Rabbits, Swine, Swine, Miniature, Bacterial Proteins genetics, CRISPR-Cas Systems genetics, Cloning, Molecular methods, Cloning, Organism methods, Endonucleases genetics, Gene Editing methods, RNA, Transfer genetics
- Abstract
CRISPR/Cpf1 features a number of properties that are distinct from CRISPR/Cas9 and provides an excellent alternative to Cas9 for genome editing. To date, genome engineering by CRISPR/Cpf1 has been reported only in human cells and mouse embryos of mammalian systems and its efficiency is ultimately lower than that of Cas9 proteins from Streptococcus pyogenes. The application of CRISPR/Cpf1 for targeted mutagenesis in other animal models has not been successfully verified. In this study, we designed and optimized a guide RNA (gRNA) transcription system by inserting a transfer RNA precursor (pre-tRNA) sequence downstream of the gRNA for Cpf1, protecting gRNA from immediate digestion by 3'-to-5' exonucleases. Using this new gRNA
tRNA system, genome editing, including indels, large fragment deletion and precise point mutation, was induced in mammalian systems, showing significantly higher efficiency than the original Cpf1-gRNA system. With this system, gene-modified rabbits and pigs were generated by embryo injection or somatic cell nuclear transfer (SCNT) with an efficiency comparable to that of the Cas9 gRNA system. These results demonstrated that this refined gRNAtRNA system can boost the targeting capability of CRISPR/Cpf1 toolkits.- Published
- 2018
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27. Effects of nitrification inhibitors on gross N nitrification rate, ammonia oxidizers, and N 2 O production under different temperatures in two pasture soils.
- Author
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Lan T, Suter H, Liu R, Yuan S, and Chen D
- Subjects
- Animals, Archaea drug effects, Archaea growth & development, Australia, Betaproteobacteria drug effects, Betaproteobacteria growth & development, Cattle, Guanidines pharmacology, Oxidation-Reduction, Pyrazoles pharmacology, Sheep, Soil Microbiology standards, Temperature, Triazoles pharmacology, Ammonia chemistry, Fertilizers analysis, Nitrification drug effects, Nitrous Oxide analysis, Soil chemistry
- Abstract
Australian pasture soil for cattle and sheep industries constitutes the principal land use with considerable N fertilizer consumption, which is one of the causes of local environmental problems. Nitrification plays a key role in regulating soil inorganic N concentration and its environmental diffusion. The effects of different nitrification inhibitors (NIs) on gross N nitrification (n
gross ) rate and N2 O production under different temperatures in pasture soils remain unclear. A laboratory incubation experiment was conducted to determine the effect of NIs (dicyandiamide [DCD], 3,4-dimethylpyrazole phosphate [DMPP], and 3-methylpyrazol and 1H-1,2,4-triazol [3MP + TZ]) on N2 O emissions, ngross and net N nitrification (nnet ) rates, and the abundance of ammonia oxidizers, namely, ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB), in two Australian pasture soils incubated at temperatures of 15, 25, and 35 °C. All NIs reduced both ngross and nnet rates and N2 O production rate from the two pasture soils but to different extents. The inhibitory rates of NIs on ngross and nnet reached 6.80-63.8 and 5.91-62.3%, respectively, whereas that on N2 O production rate totaled 4.5-41.4% in the tested soils. NIs reduced nitrification and N2 O production by inhibiting the growth of AOB rather than AOA. The inhibitory effects of NIs were temperature-dependent, that is, decreasing with increasing temperature from 15 to 35 °C. In general, DMPP performed better than DCD and 3MP + TZ at 15 and 35 °C, whereas DCD performed more effectively than the other two NIs at 25 °C. Our results suggest that the utilization of NIs will depend on the conditions present, especially soil temperature. Additionally, AOB is the target of inhibition when mitigating nitrification and N2 O emission by applying NIs in pasture soils.- Published
- 2018
- Full Text
- View/download PDF
28. The joint toxicity effect of five antibiotics and dibutyl phthalate to luminescent bacteria (Vibrio fischeri).
- Author
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Wei S, Wang F, Chen Y, Lan T, and Zhang S
- Subjects
- Toxicity Tests, Acute, Aliivibrio fischeri drug effects, Anti-Bacterial Agents toxicity, Dibutyl Phthalate toxicity, Water Pollutants, Chemical toxicity
- Abstract
Antibiotics and phthalate esters are two kinds of emerging pollutants and are ubiquitous in the aquatic ecosystem. To date, few studies analyzed the combined toxicity of the mixtures of antibiotics and phthalate esters, and their joint toxicity effect mode remains unknown. Here, we investigated the single and joint toxicity of dibutyl phthalate (DBP) and five antibiotics, namely, oxytetracycline hydrochloride (OTC), chlortetracycline hydrochloride (CTC), sulfamethazine (SMZ), sulfamerazine (SMR), and sulfadiazine (SD), to luminescent bacteria of Vibrio fischeri. The median effect concentration (EC
50 ) values of the test chemicals were ranked as CTC (6.67 mg/L) > OTC (25.12 mg/L) > SD (67.61 mg/L) > SMR (141.51 mg/L) > DBP (148.38 mg/L) > SMZ (245.07 mg/L). The joint toxicities of the binary mixtures of antibiotics and DBP were evaluated by the concentration addition (CA) and independent action (IA) models. The joint toxicity effects of CTC-DBP, OTC-DBP, SMZ-DBP, SMR-DBP, and SD-DBP all appeared to be synergism. Our study revealed that sulfonamides combined with DBP could be as toxic as or even more toxic than tetracycline. Thus, the joint toxicity effect should be considered when assessing the ecological risks of binary or multicomponent pollutants.- Published
- 2018
- Full Text
- View/download PDF
29. Clinical characteristics of hand, foot and mouth disease in Daklak Province, Vietnam and associated factors of severe cases.
- Author
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Van Pham H, Hoang TNA, Duong HT, Phan LT, Phan UTN, Ho NX, and Hoang CQ
- Abstract
The Hand, Foot and Mouth Disease (HFMD) outbreaks occurred throughout Daklak province, Vietnam in 2011. This study reviewed all 744 medical records of HFMD patients admitted to Daklak Hospital in 2011 to describe the clinical characteristics of HFMD patients and determined factors associated with severe illness. Among 744 patients, 63 (8.5%) cases were severe. Most (695, 93.4%) of the cases were 3 years old or younger, and 464 (62.4%) were boys. The number of cases peaked between August and November. Most (726, 97.6%) recovered, 17 severe cases (2.3%) were transferred to higher level hospitals, and one death. Symptoms at admission included fever (93.5% had a fever ≥ 38.5 °C), blisters (99.1%), myoclonus (58.5%), and leukocytosis (> 11,300/mm
3 : 38.8%). Viral cultures were performed for 61 of 63 severe cases, of which 26.2% were positive for Enteroviruses. Multivariable analysis found that oral ulcers (Odds Ratio (OR) 3.74; 95% Confidence Interval (CI) 2.13-6.58), myoclonus (OR 44.75; 95% CI 6.04-331.66) and high white blood cell count (OR 1.08; 95% CI 1.01-1.16 per 1000/mm3 increase) were significantly associated with severe illness. HFMD mainly occurs in children younger than 3 years old and rainy season. Oral ulcers, myoclonus, and leukocytosis should be closely monitored to promptly detect severe cases of HFMD.- Published
- 2017
- Full Text
- View/download PDF
30. Seed weight differences between wild and domesticated soybeans are associated with specific changes in gene expression.
- Author
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Yu C, Qu Z, Zhang Y, Zhang X, Lan T, Adelson DL, Wang D, and Zhu Y
- Subjects
- Gene Expression Profiling methods, Gene Ontology, Gene Regulatory Networks, Sequence Analysis, RNA, Glycine max classification, Species Specificity, Gene Expression Regulation, Plant, Seeds genetics, Glycine max genetics, Transcriptome genetics
- Abstract
Key Message: Our study systematically explored potential genes and molecular pathways as candidates for differences in seed weight resulting from soybean domestication. In addition, potential contributions of lncRNAs to seed weight were also investigated. Soybeans have a long history of domestication in China, and there are several significant phenotypic differences between cultivated and wild soybeans, for example, seeds of cultivars are generally larger and heavier than those from wild accessions. We analyzed seed transcriptomes from thirteen soybean samples, including six landraces and seven wild accessions using strand-specific RNA sequencing. Differentially expressed genes related to seed weight were identified, and some of their homologs were associated with seed development in Arabidopsis. We also identified 1251 long intergenic noncoding RNAs (lincRNAs), 243 intronic RNAs and 81 antisense lncRNAs de novo from these soybean transcriptomes. We then profiled the expression patterns of lncRNAs in cultivated and wild soybean seeds, and found that transcript levels of a number of lncRNAs were sample-specific. Moreover, gene transcript and lincRNA co-expression network analysis showed that some soybean lincRNAs might have functional roles as they were hubs of co-expression modules. In conclusion, this study systematically explored potential genes and molecular pathways as candidates for differences in seed weight resulting from soybean domestication, and will provide a useful future resource for molecular breeding of soybeans.
- Published
- 2017
- Full Text
- View/download PDF
31. The functional status of DNA repair pathways determines the sensitization effect to cisplatin in non-small cell lung cancer cells.
- Author
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Chen P, Li J, Chen YC, Qian H, Chen YJ, Su JY, Wu M, and Lan T
- Subjects
- Antineoplastic Agents therapeutic use, Blotting, Western, Cell Line, Tumor, Cisplatin therapeutic use, Comet Assay, Flow Cytometry, Fluorescent Antibody Technique, Gene Knockdown Techniques, Humans, Lung Neoplasms drug therapy, Real-Time Polymerase Chain Reaction, Signal Transduction physiology, Carcinoma, Non-Small-Cell Lung genetics, DNA Repair genetics, Drug Resistance, Neoplasm genetics, Lung Neoplasms genetics
- Abstract
Purpose: Cisplatin can cause a variety of DNA crosslink lesions including intra-strand and inter-strand crosslinks (ICLs), which are associated with the sensitivity of cancer cells to cisplatin. Here, we aimed to assess the contribution of the Fanconi anemia (FA), homologous recombination (HR) and nucleotide excision repair (NER) pathways to cisplatin resistance in non-small cell lung cancer (NSCLC)-derived cells., Methods: The expression of FA, HR and NER pathway-associated genes was assessed by RT-qPCR and Western blotting. siRNAs were used to knock down the expression of these genes. CCK-8 and flow cytometry assays were used to assess the viability and apoptotic rate of NSCLC-derived cells, respectively. Immunofluorescence and alkaline comet assays were used to assess the repair of ICLs., Results: We found that acquired cisplatin-resistant NSCLC-derived A549/DR cells exhibited markedly enhanced FA and HR repair pathway capacities compared to its parental A549 cells and another independent NSCLC-derived cell line, Calu-1, which possesses a moderate innate resistance to cisplatin. siRNA-mediated silencing of the FA-associated genes FANCL and RAD18 and the HR-associated genes BRCA1 and BRCA2 significantly potentiated the sensitivity of A549/DR cells to cisplatin compared to A549 and Calu-1 cells, suggesting that the acquired cisplatin resistance in A549/DR cells may be attributed to enhanced FA and HR pathway capacities responsible for ICL repair. Although we found that expression knockdown of the NER-associated genes XPA and ERCC1 sensitized the three NSCLC-derived cell lines to cisplatin, the sensitization effect was more significant in Calu-1 cells than in A549 and A549/DR cells, implying that the innate cisplatin resistance in Calu-1 cells may result from an increased NER activity., Conclusions: Our results indicate that the functional status of DNA repair pathways determine the sensitivity of NSCLC cells to cisplatin. Direct targeting of the pathway that is involved in cisplatin resistance may be an effective strategy to surmount cisplatin resistance in NSCLC.
- Published
- 2016
- Full Text
- View/download PDF
32. A Technical Assistance Model for Guiding Service and Systems Change.
- Author
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Le LT, Anthony BJ, Bronheim SM, Holland CM, and Perry DF
- Subjects
- Health Policy, Health Resources, Humans, Behavioral Medicine organization & administration, Decision Making, Models, Organizational
- Abstract
Technical assistance (TA) has been a ubiquitous part of the implementation of policies, programs, and services across public and private enterprise for decades. There have been few attempts to identify critical components of TA and evaluate its effectiveness. Qualitative analysis of interviews with experienced TA providers suggested a continuum of practice anchored at each end by approaches termed content-driven and relationship-based. Content-driven approaches focus on information transfer and referral whereas relationship-based approaches center on the facilitation of behavior and systems change. TA is almost always a mix of these approaches. Fitting the right approach to each situation is the key to success. The structure of TA is conceptualized as a three-phase set of activities (decision-making, implementation, and evaluation) supported by an effective partnership and informed by the overarching context. The strategies for effective TA are consistent with major theories of behavior change but need to be further evaluated and refined.
- Published
- 2016
- Full Text
- View/download PDF
33. A Rhodamine-based Dual Chemosensor for the Simultaneous Detection of Fe 3+ and Cu 2 .
- Author
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Lan T, Wang FH, Xi XJ, Cheng CW, Lei W, Xia MZ, and Wang FY
- Abstract
A Rhodamine-based dual chemosensor L1 for simultaneously detecting Fe
3+ and Cu2+ was designed and synthesized. The spectroscopic properties of L1 were analyzed, and its recognition mechanism was speculated. We found that the addition of Fe3+ induced a great fluorescence enhancement, while Cu2+ induced a strong UV-Vis absorption enhancement. The results revealed that L1 was highly selective for recognizing Fe3+ and Cu2+ in UV-Vis spectroscopy in CH3 OH-H2 O (1/1, v/v, pH 7.2) with the interference of other metal ions. A good linear relationship between the fluorescence intensities of L1 and the concentration of Fe3+ , as well as the UV-Vis absorption intensities of L1 and the concentration of Cu2+ was observed, respectively. The detection limit was 9.2 × 10-8 M (5.5 μg/L) for Fe3+ and 3.8 × 10-8 M (2.4 μg/L) for Cu2+ , respectively. The detection capacity for targeted metal ions of Fe3+ and Cu2+ were studied, which are less than 5 min. Job's plot method for L1 with Fe3+ and ESI-MS for L1 with Cu2+ indicated a 1:1 stoichiometry in the complex. The results may provide an effective strategy for the design of new dual chemosensors for the rapid detection of targeted metal ions.- Published
- 2016
- Full Text
- View/download PDF
34. Reference ranges for vertebral heights and prevalence of asymptomatic (undiagnosed) vertebral fracture in Vietnamese men and women.
- Author
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Ho-Pham LT, Mai LD, Pham HN, Nguyen ND, and Nguyen TV
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Asymptomatic Diseases epidemiology, Bone Density, Female, Fractures, Compression diagnostic imaging, Fractures, Compression epidemiology, Humans, Male, Middle Aged, Prevalence, Reference Values, Risk Factors, Spine anatomy & histology, Vietnam epidemiology, Young Adult, Arthrography standards, Osteoporosis diagnostic imaging, Osteoporosis epidemiology, Spinal Fractures diagnostic imaging, Spinal Fractures epidemiology, Spine diagnostic imaging
- Abstract
Unlabelled: Based on quantitative measurements of vertebral heights, the prevalence of undiagnosed vertebral fracture in Vietnamese men and women aged 50 years and older was 23 and 26 %, respectively., Background: The present study sought to develop reference ranges for vertebral heights and to determine the prevalence of asymptomatic vertebral fracture in Vietnamese men and women., Methods: The study included 312 men and 657 women aged over 18 who were randomly selected from the community. The ImageJ software program was used to measure anterior height (H(a)), middle height (H(m)), and posterior height (H(p)) for each vertebra (T4 to T12 and L1 to L5). Four vertebral ratios were determined: H(a)/H(p), H(m)/H(p), H(p)/H(p + 1), and H(p)/H(p − 1). Reference ranges for the ratios were then developed by the method of Winsorized mean. Vertebral fracture was diagnosed as a ratio lower than three standard deviations from the normal mean., Results: For any given vertebra, H(a), H(m), and H(p) in men were higher than in women. In both sexes, H(a) and H(m) increased in a stepwise fashion from T4 to L3 and then gradually reduced in L4–L5. Vertebral heights for T4–T9 tended to decrease, while vertebral height for T10–L5 tended to increase with advancing age. Among those aged over 50 years, the prevalence of vertebral fracture in men was 23.3 % (95 % confidence interval (CI) 16.8–31.3 %) which was lower than that in women (26.5 %; 95 % CI 22.4–31.1 %). The prevalence increased with advancing age, such that from the age of over 70, 41 % of men and 42 % women had at least one vertebral fracture., Conclusion: One fourth of Vietnamese men and women aged 50 years and older have a symptomatic vertebral fracture. This prevalence is equivalent to that in Caucasian populations.
- Published
- 2012
- Full Text
- View/download PDF
35. Template-free Synthesis of One-dimensional Cobalt Nanostructures by Hydrazine Reduction Route.
- Author
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Zhang L, Lan T, Wang J, Wei L, Yang Z, and Zhang Y
- Abstract
One-dimensional cobalt nanostructures with large aspect ratio up to 450 have been prepared via a template-free hydrazine reduction route under external magnetic field assistance. The morphology and properties of cobalt nanostructures were characterized by scanning electron microscopy, X-ray diffractometer, and vibrating sample magnetometer. The roles of the reaction conditions such as temperature, concentration, and pH value on morphology and magnetic properties of fabricated Co nanostructures were investigated. This work presents a simple, low-cost, environment-friendly, and large-scale production approach to fabricate one-dimensional magnetic Co materials. The resulting materials may have potential applications in nanodevice, catalytic agent, and magnetic recording.
- Published
- 2011
- Full Text
- View/download PDF
36. Knockdown of NtMed8, a Med8-like gene, causes abnormal development of vegetative and floral organs in tobacco (Nicotiana tabacum L.).
- Author
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Wang F, Wei H, Tong Z, Zhang X, Yang Z, Lan T, Duan Y, and Wu W
- Subjects
- Amino Acid Sequence, Cell Size, Chloroplasts metabolism, Chloroplasts ultrastructure, Fertility, Flowers anatomy & histology, Flowers cytology, Flowers ultrastructure, Gene Expression Regulation, Plant, Microscopy, Fluorescence, Molecular Sequence Data, Phylogeny, Plant Leaves cytology, Plant Leaves ultrastructure, Plant Proteins chemistry, Plant Proteins genetics, Protein Transport, RNA Interference, Sequence Alignment, Subcellular Fractions metabolism, Time Factors, Nicotiana cytology, Nicotiana ultrastructure, Flowers growth & development, Gene Knockdown Techniques, Genes, Plant genetics, Organogenesis genetics, Plant Proteins metabolism, Nicotiana genetics, Nicotiana growth & development
- Abstract
Med8, a subunit of mediator complex, has proved to possess crucial functions in many organisms from yeast to human. In plant, the med8 mutant of Arabidopsis thaliana displayed delayed anthesis and increased number of leaves during the vegetative period. However, the roles of Med8 in other flowering plants are still unknown. To investigate the function of Med8 ortholog in tobacco (Nicotiana tabacum L.; named as NtMed8), we created transgenic tobacco plants with repressed NtMed8 expression mediated by RNA interference (RNAi). Compared with the wild type, the NtMed8-RNAi plants exhibited: more leaves with smaller but thicker blades; larger cells and vascular bundles with lower stomata density in leaves; swelled chloroplasts with thicker and lumen-enlarged thylakoids; weaker root system with fewer lateral roots; larger flowers and floral organs; flowering earlier under long day, but later under short day conditions; and male sterile with larger but less germinable pollens. In addition, quantitative RT-PCR indicated that NtMed8 is expressed in both vegetative and floral tissues. Subcellular localization analysis by transient expression of fusion protein in Nicotiana benthamiana leaves showed that NtMed8 was located in both plasma membrane and nucleus. These results suggest that NtMed8 plays important roles in both vegetative and reproductive development, and the function of Med8 appears to be, at least partially, conserved in flowering plants.
- Published
- 2011
- Full Text
- View/download PDF
37. Promoting effect of layered titanium phosphate on the electrochemical and photovoltaic performance of dye-sensitized solar cells.
- Author
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Cheng P, Chen R, Wang J, Yu J, Lan T, Wang W, Yang H, Wu H, and Deng C
- Abstract
We reported a composite electrolyte prepared by incorporating layered α-titanium phosphate (α-TiP) into an iodide-based electrolyte using 1-ethyl-3-methylimidazolium tetrafluoroborate(EmimBF4) ionic liquid as solvent. The obtained composite electrolyte exhibited excellent electrochemical and photovoltaic properties compared to pure ionic liquid electrolyte. Both the diffusion coefficient of triiodide (I3-) in the electrolyte and the charge-transfer reaction at the electrode/electrolyte interface were improved markedly. The mechanism for the enhanced electrochemical properties of the composite electrolyte was discussed. The highest conversion efficiency of dye-sensitized solar cell (DSSC) was obtained for the composite electrolyte containing 1wt% α-TiP, with an improvement of 58% in the conversion efficiency than the blank one, which offered a broad prospect for the fabrication of stable DSSCs with a high conversion efficiency.
- Published
- 2010
- Full Text
- View/download PDF
38. Berberine reduces fibronectin and collagen accumulation in rat glomerular mesangial cells cultured under high glucose condition.
- Author
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Liu W, Tang F, Deng Y, Li X, Lan T, Zhang X, Huang H, and Liu P
- Subjects
- Animals, Blotting, Western, Cells, Cultured, Culture Media, Cyclic AMP Response Element-Binding Protein metabolism, Glomerular Mesangium cytology, Glomerular Mesangium metabolism, Phosphorylation, Rats, p38 Mitogen-Activated Protein Kinases metabolism, Berberine pharmacology, Collagen metabolism, Fibronectins metabolism, Glomerular Mesangium drug effects, Glucose administration & dosage
- Abstract
Diabetic nephropathy, one of the microvascular complications of diabetes mellitus, is a leading cause of end-stage renal disease. Berberine is one of the main constituents of Coptidis Rhizoma and Cortex Phellodendri. In this study, we investigated the effects of berberine on fibronectin and collagen production, and explored the role of p38MAPK signaling pathway in rat glomerular mesangial cells cultured under high glucose condition. Six groups were divided according to the different experimental conditions: (1) Normal glucose group (NG); (2) Mannitol group (Mannitol); (3) High glucose group (HG); (4) SB203580 treatment group (HG + SB203580); (5) Berberine low dosage group (HG + BBR 30 microM); (6) Berberine high dosage group (HG + BBR 90 microM). Cell proliferation and collagen synthesis were measured by MTT and (3)H-proline incorporation assay, respectively. The phospho-p38MAPK, phospho-cAMP response element binding protein (CREB) and fibronectin were detected by western blot analysis. Fibronectin protein expression and collagen synthesis were significantly increased in HG-treated group compared with normal glucose group (P < 0.05). In SB203580 treatment group and two groups of berberine, protein expression of fibronectin and collagen synthesis were obviously decreased compared with HG-treated group (P < 0.05). Berberine significantly decreased protein expression of fibronectin compared with SB203580 treatment group (P < 0.05). Berberine at high dosage significantly decreased collagen synthesis compared with SB203580 treatment group (P < 0.05). Both SB203580 and berberine significantly decreased phospho-p38MAPK and phospho-CREB level compared with HG-treated group (P < 0.05). These results indicated that berberine might inhibit fibronectin and collagen synthesis partly via p38MAPK signal pathway in rat glomerular mesangial cells exposed to high glucose.
- Published
- 2009
- Full Text
- View/download PDF
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