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The effects of different concentrations (100, 150, 200, 250 mg/L) and different particle sizes (0-75 μm, 75-120 μm, 120-150 μm, 150-500 μm) on the soluble protein content, superoxide dismutase (SOD) and catalase (CAT) activity, malondialdehyde (MDA) content, chlorophyll a (Chla) content, and photosynthetic parameters of Microcystis flos-aquae were studied, and the mechanism of the effect of suspended particulate matter on the physiology and biochemistry of Microcystis flos-aquae was discussed. The results showed that the soluble protein content of Microcystis flos-aquae did not change noticeably after being stressed by suspended particles of different concentrations/diameters. The SOD activity of Microcystis flos-aquae first increased and then decreased with increasing suspended particulate matter concentrations. The SOD activity of Microcystis flos-aquae reached 28.03 U/mL when the concentration of suspended particulate matter was 100 mg/L. The CAT activity of Microcystis flos-aquae increased with increasing concentrations of suspended particles and reached a maximum value of 12.45 U/mg prot in the 250 mg/L concentration group, showing a certain dose effect. Small particles had a more significant effect on SOD, CAT, and MDA in Microcystis flos-aquae than large particles. The larger the concentration was and the smaller the particle size was, the stronger the attenuation of light and the lower the content of Chla. Both the maximum quantum yield of PSII (Fv/Fm) and the potential photosynthetic activity of PSII (Fv/F 0 ) of Microcystis flos-aquae increased at first and then decreased under different concentrations/sizes of suspended particles. The relative electron transfer rate gradually returned to a normal level over time. There was no significant difference in the initial slope (α) value between the treatment group and the control group, and the maximum photo synthetic rate (ETRmax) and the semilight saturation (I k ) decreased., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Natural nanoparticles (NNP) are ubiquitous in natural water and can interact with other contaminants, causing ecotoxic effects on aquatic nontarget organisms. However, the impact of NNPs on the ecotoxicity of antibiotics remains largely unknown. This work investigated the acute toxicity, chronic effect, and oxidative response and damage in Daphnia magna co-exposed to phenicol antibiotics (chloramphenicol, thiamphenicol) and different concentrations of NNPs (10 mg/L: environmentally relevant concentration; 100 mg/L: a high concentration that caused no apparent immobilization in D. magna). The results showed that the acute toxicity of chloramphenicol was increased by 10 mg/L NNPs but decreased by 100 mg/L NNPs; both concentrations of NNPs increased and decreased acute toxicities of thiamphenicol and chloramphenicol + thiamphenicol treatments, respectively. After long-term exposure, phenicol antibiotics (1 μg/L) and NNP (10 mg/L) mixtures in environmentally relevant concentrations significantly affected the reproduction of D. magna but did not influence their growth. The catalase activity, reduced glutathione level, and malonaldehyde content in D. magna also varied with the NNPs concentrations. Notably, the lowest concentration of thiamphenicol and chloramphenicol + thiamphenicol combined with NNPs significantly increased the malondialdehyde content in D. magna compared with the control, indicating membrane lipid peroxidation occurred in daphnids. This study suggests that the toxic effects of contaminants and NNPs on aquatic organisms should be considered thoroughly to avoid underestimating the hazard of these pollutants in the actual aquatic environment., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Biosynthetic of silver nanoparticles (AgNPs) by using fungi has attracted much attention due to its high catalytic efficiency and environmentally friendly characteristic. However, a few studies have focused on the ecological toxicity effects of biogenic AgNPs on algae. Here, we first investigated the catalytic reduction of 4-nitrophenol (4-NP) by WZ07-AgNPs biosynthesized by Letendraea sp. WZ07. WZ07-AgNPs had significant catalytic activity with 97.08% degradation of 4-NP in 3.5 min. Then, the toxic effects of WZ07-AgNPs and commercial-AgNPs were compared by Chlorella pyrenoidosa growth, chlorophyll content, protein content, physiological, and biochemical indexes. The results demonstrated that the algal cell biomass of C. pyrenoidosa was differentially inhibited after exposure to different concentrations of AgNPs, which showed concentration dependence and time dependence. The 96h-EC 50 values of WZ07-AgNPs and commercial-AgNPs on C. pyrenoidosa were 15.99 mg/mL and 12.69 mg/mL, respectively. With the increase concentration of AgNPs, the chlorophyll content was gradually decreased, the protein content was first increased and then decreased, the activities of superoxide dismutase (SOD) and catalase (CAT) were decreased, and the level of malondialdehyde (MDA) was increased significantly of C. pyrenoidosa. In general, AgNPs affect the growth of algae to some extent. However, compared with commercial-AgNPs, WZ07-AgNPs is less toxic to C. pyrenoidosa, which could be used as a potential and an eco-friendly catalyst. This study provides a basis for the safe application of biosynthetic AgNPs., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

The seasonal and spatial variation in the phytoplankton community structure and the environmental variables were investigated in December (the dry season) 2016 and July (the rainy season) 2017 in the Jinjiang River Estuary, China. We identified a total of 138 species of phytoplankton, which were mainly Chlorophyta, Bacillariophyta, and Cryptophyta in the dry season; however, in the rainy season, only Bacillariophyta were found. In the Jinjiang River Estuary, the species evenness and the biodiversity index were higher in the rainy season and that the species diversity was higher in the dry season. Redundancy analysis (RDA) revealed that the dominant species were statistically related to many of the environmental variables, including the water temperature (WT), pH, salinity (Sal), dissolved oxygen (DO), total phosphorus (TP), and total nitrogen (TN). Among the variables, the Sal, DO, TP, and TN had a significant influence on the dominant species distribution, and the WT and pH also affected the dominant species distribution to some extent., (© 2021. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

The effects of chitosan, gallic acid, and algicide chitosan-gallate on the activities of antioxidant enzymes, malonaldehyde (MDA) content, and photosynthetic activity of Microcystis flos-aquae were investigated to explore the physiological and biochemical mechanisms of algicides. Results demonstrated that chitosan did not significantly affect catalase (CAT) and superoxide dismutase (SOD) activities, MDA content, and photosynthetic activity in this alga. At 30 mg L(-1), gallic acid, CAT, and SOD activities and MDA of M. flos-aquae cells showed maximums of 2.872 × 10(-10) mg·cell(-1) min(-1), 0.787 × 10(-8) U·cell(-1), and 0.626 × 10(-8) nmol·cell(-1), respectively. Photosynthetic organs in algal cells were severely damaged under the stress of high gallic acid concentrations, inducing blockage of photosynthetic electron transport and resulting in the inability to restore normal photosynthetic activity. CAT and SOD activities and MDA content with lower algicide concentration were significantly higher than the control group (p < 0.05) and, in higher algicide groups, significantly lower than the control (p < 0.05). Algicide releasing gallic acid in groups treated with 60, 90, and 130 mg/L algicide was strong enough to cause severe damage to photosynthetic organs in these algal cells. The algicide suppression time was longer than that of directly added gallic acid.

The effects of levofloxacin (LEV) on Microcystis flos-aquae and its mechanism were investigated by determining the responses of some parameters of M. flos-aquae to LEV stress, including growth inhibition ratio, chlorophyll a content, superoxide dismutase (SOD) and catalase (CAT) activities, malondialdehyde (MDA) content, F v/F 0 and F v/F m, etc. The results indicated that LEV at 0.001-0.1 μg L(-1) could stimulate the growth of M. flos-aquae and increase the chlorophyll a content but did not induce a significant increase in the activity of antioxidant enzymes (SOD and CAT) and the content of MDA. When the LEV concentration exceeds 10 μg L(-1), the growth of M. flos-aquae could be significantly inhibited (the highest inhibition ratio can be up to 88.38 % at 100 μg L(-1)) and chlorophyll a content, SOD and CAT activities, and MDA content also significantly decreased in a concentration-dependent manner, indicating that high concentrations of LEV caused a severe oxidative stress on algal cells, resulting in a large number of reactive oxygen species produced in algal cells and thereby inhibiting the growth of algae. At the same time, the F v/F m and F v/F 0 values of M. flos-aquae decreased significantly with both exposure time and increasing test concentration of LEV, showing that the process of photosynthesis was inhibited.

Background, Aim, and Scope: Fujian reservoirs in southeast China are important water resources for economic and social sustainable development, although few have been studied previously. In recent years, growing population and increasing demands for water shifted the focus of many reservoirs from flood control and irrigation water to drinking water. However, most of them showed a rapid increase in the level of eutrophication, which is one of the most serious and challenging environmental problems. In this study, we investigated the algae community characteristics, trophic state, and eutrophication control strategies for typical subtropical reservoirs in southeast Fujian., Materials and Methods: Surface water samples were collected using polyvinyl chloride (PVC) plastic bottles from 11 Fujian reservoirs in summer 2010. Planktonic algae were investigated by optical microscopy. Water properties were determined according to the national standard methods., Results and Discussion: Shallow reservoirs generally have higher values of trophic state index (TSI) and appear to be more susceptible to anthropogenic disturbance than deeper reservoirs. A total of 129 taxa belonging to eight phyla (i.e., Bacillariophyta, Chlorophyta, Chrysophyta, Cryptophyta, Cyanophyta, Euglenophyta, Pyrrophyta, Xanthophyta) were observed and the most diverse groups were Chlorophyta (52 taxa), Cyanophyta (20 taxa), Euglenophyta (17 taxa), Chrysophyta (14 taxa). The dominant groups were Chlorophyta (40.58%), Cyanophyta (22.91%), Bacillariophyta (21.61%), Chrysophyta (6.91%). The species richness, abundance, diversity, and evenness of algae varied significantly between reservoirs. TSI results indicated that all 11 reservoirs were eutrophic, three of them were hypereutrophic, six were middle eutrophic, and two were light eutrophic. There was a strong positive correlation between algal diversity and TSI at P < 0.05. Our canonical correspondence analysis (CCA) results illustrated that temperature, transparency, conductivity, DO, TC, NH(4)-N, NO(x)-N, TP, and chlorophyll a were significant environmental variables affecting the distribution of algae communities. The transparency and chlorophyll a were the strongest environmental factors in explaining the community data. Furthermore, the degradation of water quality associated with excess levels of nitrogen and phosphorus in Fujian reservoirs may be impacted by interactions among agriculture and urban factors. A watershed-based management strategy, especially phosphorus control, should be developed for drinking water source protection and sustainable reservoirs in the future., Conclusion and Recommendations: All investigated reservoirs were eutrophicated based on the comprehensive TSI values; thus, our results provided an early warning of water degradation in Fujian reservoirs. Furthermore, the trophic state plays an important role in shaping community structure and in determining species diversity of algae. Therefore, long-term and regular monitoring of Euglenophyta, Cyanophyta, TN, TP and chlorophyll a in reservoirs is urgently needed to further understand the future trend of eutrophication and to develop a watershed-based strategy to manage the Cyanophyta bloom hazards.